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The map label for this gene is fabI [H]

Identifier: 89256735

GI number: 89256735

Start: 1369371

End: 1370153

Strand: Reverse

Name: fabI [H]

Synonym: FTL_1442

Alternate gene names: 89256735

Gene position: 1370153-1369371 (Counterclockwise)

Preceding gene: 89256740

Following gene: 89256733

Centisome position: 72.27

GC content: 36.91

Gene sequence:

>783_bases
ATGGGTTTTCTAGCAGGAAAAAAAATATTAATCACTGGACTTTTAAGTAATAAGTCAATTGCATATGGTATTGCTAAAGC
TATGCATAGAGAGGGAGCCGAGCTTGCTTTTACTTATGTTGGACAGTTCAAAGATAGAGTGGAAAAATTATGTGCAGAAT
TTAATCCAGCTGCAGTTTTGCCTTGCGATGTGACTTCTGATAAAGAGATTAAGGATTTATTTGTAGAGCTAGGTAAAGTT
TGGGATGGTCTAGATGCCATAGTTCATTCTATAGCTTTTGCACCGCGTGATCAGTTAGAAGGTAACTTTATTGACTGTGT
AACTCGCGAGGGTTTTAGTATCGCTCATGATATTAGTGCCTATTCTTTTGCAGCATTAGCTAAAGAAGGTCGTAGTATGA
TGAAAAATCGTAATGCTTCTATGGTAGCACTTACTTATATTGGAGCAGAAAAAGCTATGCCAAGTTACAATACTATGGGT
GTTGCTAAAGCATCTCTAGAAGCTACAGTTAGATATACAGCGTTAGCTTTAGGTGAGGATGGTATCAAGGTAAATGCTGT
ATCAGCTGGTCCTATCAAAACTCTGGCAGCTTCTGGTATATCAAACTTCAAGAAGATGCTTGATTATAATGCTATGGTTT
CTCCACTTAAGAAAAATGTTGATATTATGGAAGTTGGTAATACTGTAGCGTTTTTATGTTCAGATATGGCAACTGGTATC
ACTGGAGAAGTTGTCCATGTTGATGCTGGATATCATTGTGTGTCTATAGGTAATGTTCTTTAA

Upstream 100 bases:

>100_bases
GTTAGCTTTGTAATTTAAAAATAAAAGAGCGTTGTATAACTATTTATGAAATTATTAGTAACTAAGATTTTAACAATAAA
TCCACAAAGGAGATGTAAAT

Downstream 100 bases:

>100_bases
TTTTCTAAATTAATCTGTTTCCAATCAATTAGATTTATTTGTATCTATATAATAAAAACTGATATATTTTATAAAAAATA
TTAGGTTCTGTGCACAAAAA

Product: enoyl-[acyl-carrier-protein] reductase (NADH)

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVLPCDVTSDKEIKDLFVELGKV
WDGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISAYSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMG
VAKASLEATVRYTALALGEDGIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGI
TGEVVHVDAGYHCVSIGNVL

Sequences:

>Translated_260_residues
MGFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVLPCDVTSDKEIKDLFVELGKV
WDGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISAYSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMG
VAKASLEATVRYTALALGEDGIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGI
TGEVVHVDAGYHCVSIGNVL
>Mature_259_residues
GFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVLPCDVTSDKEIKDLFVELGKVW
DGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISAYSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMGV
AKASLEATVRYTALALGEDGIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGIT
GEVVHVDAGYHCVSIGNVL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=59.9206349206349, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=28.515625, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082100, Length=272, Percent_Identity=25, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27775; Mature: 27644

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVL
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCEEE
PCDVTSDKEIKDLFVELGKVWDGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCHHHHHHH
YSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMGVAKASLEATVRYTALALGED
HHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCCCHHHHHHHHHHHEEEEEEECCC
GIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCC
TGEVVHVDAGYHCVSIGNVL
CCEEEEEECCCEEEECCCCC
>Mature Secondary Structure 
GFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVL
CCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCEEE
PCDVTSDKEIKDLFVELGKVWDGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCHHHHHHH
YSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMGVAKASLEATVRYTALALGED
HHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCCCHHHHHHHHHHHEEEEEEECCC
GIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCC
TGEVVHVDAGYHCVSIGNVL
CCEEEEEECCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10464225; 10984043 [H]