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The map label for this gene is ybiK [C]

Identifier: 89256711

GI number: 89256711

Start: 1348020

End: 1348883

Strand: Reverse

Name: ybiK [C]

Synonym: FTL_1418

Alternate gene names: 89256711

Gene position: 1348883-1348020 (Counterclockwise)

Preceding gene: 89256712

Following gene: 89256710

Centisome position: 71.14

GC content: 36.57

Gene sequence:

>864_bases
ATGCAAAAAATTATAATCCATGGTGGTTGTGGTGCTAGAGAAGACAAAAATACTTCTTTTGGAGATTATCACCAGCATCT
TTTACCAATTGTTGAAAAAGCATATAACTACTTAAAAGAAGTAGATGATGCTAATGAGGCAGCAATTTTTGCTGCTAAAC
TTCTAGAGGATGATGAGATATTTAATGCTGGTACTGGTTCGCGCGTGCAGCAAGATGGACAAATCAGGATGTCTGCATCT
ATCATCGATAGTCAAAAACAAAAGTTTGCTGGTGTTATAAATATTCAAAATATCAAAAATCCAATAGAAGTTGCAAATAG
ATTAATGCAACAGCATCATAGCATCCTTGCAGGTGATCAAGCTACGACATTCGCTCATGATGTGATGGGTTTACCAATTT
ATAACCCGATGACAGAAAAAAGATATCAAGAATGCCTACAGCTTAAAAAAGGCTATACAGGAACGATAGGTGTAGTAGCT
TTAGACTCAAAAGGAAAAATTTGTGCAGTAACCTCAACAGGTGGTGCTGGATTTGAATATCCAGGAAGAGTAGGAGATAG
TCCGACAGTTGCGGGTAATTTTGCTAATGAGTGTATGGGAATATCGTGTACAGGGATAGGTGAGCACATAATTAATCAAG
CAGTTGCGGCAAAGATTGCTACTCGAGTTAAAGATGGAATGTCTTTGTTGGCTGCCGTAGATAAATCTATAGCAGAAAGT
GATAGTTTGGGCGATTATGTTGGACTTATAGCTATTGATAAACATGGAAATATTTGCTCTGGATCAACTTCTATAGCGCA
AACATTATATGCCTATGCTGATGGTGAACAGATAAAAACATTTTATCAAGAAAAAATGTTATAA

Upstream 100 bases:

>100_bases
TTTATATTTTTGACTTAACCTAATTACTTACTCATATGAGTTTAATAGTAATTTAGCTATAATCTATTTGATGTAACTAA
GTTTATAAACAGGAGATATT

Downstream 100 bases:

>100_bases
AATACTTAATAAAAGTATTGACTTGAATATGTCTGTATATGTATAATACCTGTCATTGGCCAGATAGCTCAGTCGGTAGA
GCAGAGGACTGAAAATCCTC

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MQKIIIHGGCGAREDKNTSFGDYHQHLLPIVEKAYNYLKEVDDANEAAIFAAKLLEDDEIFNAGTGSRVQQDGQIRMSAS
IIDSQKQKFAGVINIQNIKNPIEVANRLMQQHHSILAGDQATTFAHDVMGLPIYNPMTEKRYQECLQLKKGYTGTIGVVA
LDSKGKICAVTSTGGAGFEYPGRVGDSPTVAGNFANECMGISCTGIGEHIINQAVAAKIATRVKDGMSLLAAVDKSIAES
DSLGDYVGLIAIDKHGNICSGSTSIAQTLYAYADGEQIKTFYQEKML

Sequences:

>Translated_287_residues
MQKIIIHGGCGAREDKNTSFGDYHQHLLPIVEKAYNYLKEVDDANEAAIFAAKLLEDDEIFNAGTGSRVQQDGQIRMSAS
IIDSQKQKFAGVINIQNIKNPIEVANRLMQQHHSILAGDQATTFAHDVMGLPIYNPMTEKRYQECLQLKKGYTGTIGVVA
LDSKGKICAVTSTGGAGFEYPGRVGDSPTVAGNFANECMGISCTGIGEHIINQAVAAKIATRVKDGMSLLAAVDKSIAES
DSLGDYVGLIAIDKHGNICSGSTSIAQTLYAYADGEQIKTFYQEKML
>Mature_287_residues
MQKIIIHGGCGAREDKNTSFGDYHQHLLPIVEKAYNYLKEVDDANEAAIFAAKLLEDDEIFNAGTGSRVQQDGQIRMSAS
IIDSQKQKFAGVINIQNIKNPIEVANRLMQQHHSILAGDQATTFAHDVMGLPIYNPMTEKRYQECLQLKKGYTGTIGVVA
LDSKGKICAVTSTGGAGFEYPGRVGDSPTVAGNFANECMGISCTGIGEHIINQAVAAKIATRVKDGMSLLAAVDKSIAES
DSLGDYVGLIAIDKHGNICSGSTSIAQTLYAYADGEQIKTFYQEKML

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Probably performs the final step in the degradation of the reserve polymer cyanophycin (depolymerizes the building block L-beta-Asp-Arg). Also has L- asparagin

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=290, Percent_Identity=32.7586206896552, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI145275200, Length=290, Percent_Identity=32.7586206896552, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI98991769, Length=263, Percent_Identity=26.9961977186312, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1787050, Length=310, Percent_Identity=34.1935483870968, Blast_Score=130, Evalue=9e-32,
Organism=Drosophila melanogaster, GI18921183, Length=298, Percent_Identity=29.8657718120805, Blast_Score=100, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 30900; Mature: 30900

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIIIHGGCGAREDKNTSFGDYHQHLLPIVEKAYNYLKEVDDANEAAIFAAKLLEDDEI
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
FNAGTGSRVQQDGQIRMSASIIDSQKQKFAGVINIQNIKNPIEVANRLMQQHHSILAGDQ
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCEEEHHHCCCHHHHHHHHHHHHHHHHCCCC
ATTFAHDVMGLPIYNPMTEKRYQECLQLKKGYTGTIGVVALDSKGKICAVTSTGGAGFEY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCC
PGRVGDSPTVAGNFANECMGISCTGIGEHIINQAVAAKIATRVKDGMSLLAAVDKSIAES
CCCCCCCCCCCCCCHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSLGDYVGLIAIDKHGNICSGSTSIAQTLYAYADGEQIKTFYQEKML
CCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQKIIIHGGCGAREDKNTSFGDYHQHLLPIVEKAYNYLKEVDDANEAAIFAAKLLEDDEI
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
FNAGTGSRVQQDGQIRMSASIIDSQKQKFAGVINIQNIKNPIEVANRLMQQHHSILAGDQ
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCEEEHHHCCCHHHHHHHHHHHHHHHHCCCC
ATTFAHDVMGLPIYNPMTEKRYQECLQLKKGYTGTIGVVALDSKGKICAVTSTGGAGFEY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCC
PGRVGDSPTVAGNFANECMGISCTGIGEHIINQAVAAKIATRVKDGMSLLAAVDKSIAES
CCCCCCCCCCCCCCHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSLGDYVGLIAIDKHGNICSGSTSIAQTLYAYADGEQIKTFYQEKML
CCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]