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The map label for this gene is glyS [H]

Identifier: 89256651

GI number: 89256651

Start: 1283273

End: 1285357

Strand: Reverse

Name: glyS [H]

Synonym: FTL_1350

Alternate gene names: 89256651

Gene position: 1285357-1283273 (Counterclockwise)

Preceding gene: 89256655

Following gene: 89256648

Centisome position: 67.79

GC content: 33.14

Gene sequence:

>2085_bases
ATGAGTAAAATTACAAAAGATTTTTTATTTGAATTGGGTACTGAAGAGTTACCACCAAAAGCACTAAGAAATTTAGCCCA
GTCTTTATTAGTCAGTGTTGAAAGTCAGCTAAAAGAGGCAGAAGTTAGTTTTGGTGATACTAAATGGTTCGCTTCACCAA
GAAGGTTATCTTTTATAATCAAAGGTCTAGCAGAGTCTCAACAAGATGTAGTTATTGAAAAGCAAGGACCGTTGGTAAGT
ATTGCTTATAAGGAAGGTGAACCTACTCAAGTAGGTTTAGGTTTTGCAAAATCTTGTGGTGTTGAGCTTGATGAATTAGA
AAGGGTAGCTACACCAAAAGGTGATAAGCTTTTTTATAAAACAGTACAATCTGGTCAAGCAACAGTTAATTTATTGCAAG
AAATTATTACCAAAGCGCTCAAACAATTACCAATTTCAAAGATGATGCGTTGGGGCTCTTCAAGTGTTGAGTTTGTAAGA
CCTGTACATTGGGTATTAGCATTATATGGTAACGATGTTGTCGATATAGAAATTTTAGGACATAAAGCTGCTAATATTAC
ATATGGACACAGATTCCATCACCCTCAAGCTATAGTAATTGATAATATTAGTGATTATATCAAGCTACTTGCTGATGCTA
TGGTGATTGTAGATTGGCAACAGCGTAAACAAATGTTAGTTGAACAAGCTGAAAATATTGCTAAAGAAAATGATTATCAA
GTAGTTCTAGACGATGATTTAGTCGAAGAGGTTTGTGCTATAGTTGAGTACCCAAACGCAATGTTATGCAGTTTTAACAA
GGATTTCCTAAGAGTTCCTCAAGAAGCGTTAATATCTGCTATGGAAGAACATCAAAAGTGTTTTGCTCTATTGGATAATC
AGGGTGATTTGGTTGCTAATTTTATTACAATTTCAAATATCCAAAGTAAAAAGCCAGAACTAGTAACATCAGGTAATCAA
AAGGTAATGAATGCTAGGCTGGCAGATGCGGCTTTTTTCTATGATACTGATTTGAAGACTTCTTTAGAACAGTTGTTACC
TAAGCTTGAGCATGTAACTTTCCAAAGTAAACTTGGTAATATGTATCAGAAAGCTCAAAGAATAGCTAATATAGCACAAC
AGTTAGCAGAACTAGGTAATTTTGATTCTCAACAAGCACATAGGGCGGGTCTTTTAGCTAAAGCTGACTTAATTTCAAAT
ATGGTGTTTGAGTTTACAGATTTACAAGGAATTATTGGTAAATATTACGCAAAAGCTCATGGTGAAACAGATACAGTAGC
CGAGGCAATAGAGCAACAATATTGGCCAAAGTATTCTGGAGCGGAGTTACCAAGAACTAATGTTGCTGCATGTGTAGCAT
TAGCTGAGAAATTAGATACTTTAGTAGGTATATTTGCAATTGGTCAGAAACCAACAGGAAATAAAGATCCATTTGCATTA
AGAAGATCAGCTATAGGTATCTTGCGCATACTAAGAGATACAAGTGTTGATATATCTTTAGAAAAGATTATTGATATAAC
TTTAGAAAGTTATAAAAAAATTAATAATTTAGAGTTTAATACTGATGTAAAAACAGAAGTAATATCTTTCTGTCTAGATA
GACTAAAAAACCTTTATAAAGAAGAGGGTATTGCTGTAGATATTTTTGAAGCTATTAATAATACTAATTATGATTCGATC
AAAGATTTTGCTGCGAGAGTAGAAGCTGTAACTAAGTTCTACAATTCAGATAAAGCTCAGAGTCTAATAGCTTCAAATAA
GCGTGTAGCTAATATTATTAGTAAGAATGCTACAGATAAAGATTACTATTATAATATTGAACTTGCTAAAGCAGCTGCTA
ATGAATATGAGTTAGCACTTGCTTATAGTATCGAAGAGGTTGCTGCTGATCTTCATAAGTACCTTAATAATCGAGAATAT
AGTTATGCTCTAGAGCTGTTGACTTGTCTTGATAAAGTGATTAGTGAGTTTTTTGAGAATGTAATGGTAATTGATGAAGA
TATTAAGATAAGAGAAAATAGACTTGCTTTGCTTGTAAACCTGCATAAGATGTTCATTGGAATTGCTGATATCTCTAAAT
TATAG

Upstream 100 bases:

>100_bases
GGATCTCAATATAATTGATATTTGTCGTGAGATGCTGAAATTAATTTAGCATGACATTTTAGTGTTAATGAAAATAAACA
TTTATCTATAAGGTTTGTAA

Downstream 100 bases:

>100_bases
GTATATCGAAAATTACTAGATTTTATTCTAGATAGCTAGGCTATATTATGATTGTTATTTAAAAAAAATAGCCAAAGCTT
ATATATAGTTAATCCGAAAG

Product: glycyl-tRNA synthetase beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MSKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFIIKGLAESQQDVVIEKQGPLVS
IAYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYKTVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVR
PVHWVLALYGNDVVDIEILGHKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQRKQMLVEQAENIAKENDYQ
VVLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVANFITISNIQSKKPELVTSGNQ
KVMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGNMYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISN
MVFEFTDLQGIIGKYYAKAHGETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFAL
RRSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYKEEGIAVDIFEAINNTNYDSI
KDFAARVEAVTKFYNSDKAQSLIASNKRVANIISKNATDKDYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREY
SYALELLTCLDKVISEFFENVMVIDEDIKIRENRLALLVNLHKMFIGIADISKL

Sequences:

>Translated_694_residues
MSKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFIIKGLAESQQDVVIEKQGPLVS
IAYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYKTVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVR
PVHWVLALYGNDVVDIEILGHKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQRKQMLVEQAENIAKENDYQ
VVLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVANFITISNIQSKKPELVTSGNQ
KVMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGNMYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISN
MVFEFTDLQGIIGKYYAKAHGETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFAL
RRSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYKEEGIAVDIFEAINNTNYDSI
KDFAARVEAVTKFYNSDKAQSLIASNKRVANIISKNATDKDYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREY
SYALELLTCLDKVISEFFENVMVIDEDIKIRENRLALLVNLHKMFIGIADISKL
>Mature_693_residues
SKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFIIKGLAESQQDVVIEKQGPLVSI
AYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYKTVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVRP
VHWVLALYGNDVVDIEILGHKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQRKQMLVEQAENIAKENDYQV
VLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVANFITISNIQSKKPELVTSGNQK
VMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGNMYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISNM
VFEFTDLQGIIGKYYAKAHGETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFALR
RSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYKEEGIAVDIFEAINNTNYDSIK
DFAARVEAVTKFYNSDKAQSLIASNKRVANIISKNATDKDYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREYS
YALELLTCLDKVISEFFENVMVIDEDIKIRENRLALLVNLHKMFIGIADISKL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=691, Percent_Identity=41.6787264833575, Blast_Score=537, Evalue=1e-154,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 78023; Mature: 77892

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFII
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHH
KGLAESQQDVVIEKQGPLVSIAYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYK
HHHHCCCCCEEEECCCCEEEEEECCCCCCEECCCHHHHCCCCHHHHHHHHCCCCCHHHHH
TVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVRPVHWVLALYGNDVVDIEILG
HHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
HKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQRKQMLVEQAENIAKENDYQ
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCCE
VVLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVAN
EEECHHHHHHHHHHHHCCCHHEECCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEH
FITISNIQSKKPELVTSGNQKVMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGN
HEEECCCCCCCCCCEECCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
MYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISNMVFEFTDLQGIIGKYYAKAH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFAL
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RRSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYK
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGIAVDIFEAINNTNYDSIKDFAARVEAVTKFYNSDKAQSLIASNKRVANIISKNATDK
HCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCCC
DYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREYSYALELLTCLDKVISEFFEN
CEEEEEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
VMVIDEDIKIRENRLALLVNLHKMFIGIADISKL
CEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFII
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHH
KGLAESQQDVVIEKQGPLVSIAYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYK
HHHHCCCCCEEEECCCCEEEEEECCCCCCEECCCHHHHCCCCHHHHHHHHCCCCCHHHHH
TVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVRPVHWVLALYGNDVVDIEILG
HHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
HKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQRKQMLVEQAENIAKENDYQ
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCCE
VVLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVAN
EEECHHHHHHHHHHHHCCCHHEECCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEH
FITISNIQSKKPELVTSGNQKVMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGN
HEEECCCCCCCCCCEECCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
MYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISNMVFEFTDLQGIIGKYYAKAH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFAL
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RRSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYK
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGIAVDIFEAINNTNYDSIKDFAARVEAVTKFYNSDKAQSLIASNKRVANIISKNATDK
HCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCCC
DYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREYSYALELLTCLDKVISEFFEN
CEEEEEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
VMVIDEDIKIRENRLALLVNLHKMFIGIADISKL
CEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA