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The map label for this gene is exoA [H]

Identifier: 89256562

GI number: 89256562

Start: 1189794

End: 1190582

Strand: Direct

Name: exoA [H]

Synonym: FTL_1244

Alternate gene names: 89256562

Gene position: 1189794-1190582 (Clockwise)

Preceding gene: 89256559

Following gene: 89256563

Centisome position: 62.75

GC content: 33.84

Gene sequence:

>789_bases
ATGATAAAAGTAATGACTTTTAATGCCAATGGTATACGTGCAGCTGCTCGTAAAGGATTTTGGGAGTGGTTTAAAACTCA
AGATGTAGATTTTTTGTGTATGCAAGAAACAAAAGCCCAATTTCATCAATTAGAGAAAGACATAGAACACTTCCCTACTG
GTTACTATTATGAATTTAAAGATGCTGTAAAAAAAGGTTATAGCGGTACAGCTATCTACGCCAAGAAAAAACCTCTAAAA
GTAATCAAAGAGCTTGGATTAGATTGGGCAGATGATGAAGGTCGTTATATTCAATTTGATTATGAAAATTTTAGTATTGC
TAGTTTATATTTGCCAAGTGGCTCTAGTGGAGATGTTCGCCAAGAGTATAAGATGCAGTTTCTTAAAAAATACAAAGAAA
TATTAAAAGAACAAGTTGAATCTGGTAGAGACTTTATAGTTTGTGGTGATTTTAATATTGTGCATAAAGAGATTGATATC
AAAAACTGGAAATCTAATTATGGCAAGACATCTGGGGTATTACCAGAAGAGCAAGCTTGGTTAGATCATATTTTTGATGA
TCTAGGTTGGGTTGATACTTTCCGAGTAATCAATCATGAACCGTTACAATACACTTGGTGGTCAAATCGTGGTCAGGCTA
GAGCAAACAATGTTGGCTGGAGAATTGATTATCATATCTCAACATCTGCTCTAAAAGATAAGGTTGTGCCAGAGTCTGAT
TATGTCTATAAAGAGAATTGGTTCTCAGATCATGCACCATTAACTATTAATTATGATTATGAGGTTTAA

Upstream 100 bases:

>100_bases
AAAGTTTTATTAATTTGATAAGTAATCGTTAGTTATTACACAGCTGGTGTTAAAATGCTAAAATGATTTTAATAATTAAG
TTATCAAAATAATAAACCCT

Downstream 100 bases:

>100_bases
TCTACCAAGGAGCTCTTAAAATGTCTAATAGAATTGTTTTAATTACAGGTGCCACAAAAGGAATTGGATTAGCTACAAGC
TTATATTTAAATAATCAAGG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MIKVMTFNANGIRAAARKGFWEWFKTQDVDFLCMQETKAQFHQLEKDIEHFPTGYYYEFKDAVKKGYSGTAIYAKKKPLK
VIKELGLDWADDEGRYIQFDYENFSIASLYLPSGSSGDVRQEYKMQFLKKYKEILKEQVESGRDFIVCGDFNIVHKEIDI
KNWKSNYGKTSGVLPEEQAWLDHIFDDLGWVDTFRVINHEPLQYTWWSNRGQARANNVGWRIDYHISTSALKDKVVPESD
YVYKENWFSDHAPLTINYDYEV

Sequences:

>Translated_262_residues
MIKVMTFNANGIRAAARKGFWEWFKTQDVDFLCMQETKAQFHQLEKDIEHFPTGYYYEFKDAVKKGYSGTAIYAKKKPLK
VIKELGLDWADDEGRYIQFDYENFSIASLYLPSGSSGDVRQEYKMQFLKKYKEILKEQVESGRDFIVCGDFNIVHKEIDI
KNWKSNYGKTSGVLPEEQAWLDHIFDDLGWVDTFRVINHEPLQYTWWSNRGQARANNVGWRIDYHISTSALKDKVVPESD
YVYKENWFSDHAPLTINYDYEV
>Mature_262_residues
MIKVMTFNANGIRAAARKGFWEWFKTQDVDFLCMQETKAQFHQLEKDIEHFPTGYYYEFKDAVKKGYSGTAIYAKKKPLK
VIKELGLDWADDEGRYIQFDYENFSIASLYLPSGSSGDVRQEYKMQFLKKYKEILKEQVESGRDFIVCGDFNIVHKEIDI
KNWKSNYGKTSGVLPEEQAWLDHIFDDLGWVDTFRVINHEPLQYTWWSNRGQARANNVGWRIDYHISTSALKDKVVPESD
YVYKENWFSDHAPLTINYDYEV

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=31.7829457364341, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=31.7829457364341, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=31.7829457364341, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=27.9850746268657, Blast_Score=84, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71989536, Length=260, Percent_Identity=30.3846153846154, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI221330655, Length=256, Percent_Identity=32.8125, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17136678, Length=256, Percent_Identity=32.8125, Blast_Score=134, Evalue=5e-32,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30908; Mature: 30908

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVMTFNANGIRAAARKGFWEWFKTQDVDFLCMQETKAQFHQLEKDIEHFPTGYYYEFK
CEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEHH
DAVKKGYSGTAIYAKKKPLKVIKELGLDWADDEGRYIQFDYENFSIASLYLPSGSSGDVR
HHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHH
QEYKMQFLKKYKEILKEQVESGRDFIVCGDFNIVHKEIDIKNWKSNYGKTSGVLPEEQAW
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCHHHCCCCCCCCCCCCHHHH
LDHIFDDLGWVDTFRVINHEPLQYTWWSNRGQARANNVGWRIDYHISTSALKDKVVPESD
HHHHHHHHCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEEEEHHHHHHCCCCCCC
YVYKENWFSDHAPLTINYDYEV
CEEECCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MIKVMTFNANGIRAAARKGFWEWFKTQDVDFLCMQETKAQFHQLEKDIEHFPTGYYYEFK
CEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEHH
DAVKKGYSGTAIYAKKKPLKVIKELGLDWADDEGRYIQFDYENFSIASLYLPSGSSGDVR
HHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHH
QEYKMQFLKKYKEILKEQVESGRDFIVCGDFNIVHKEIDIKNWKSNYGKTSGVLPEEQAW
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCHHHCCCCCCCCCCCCHHHH
LDHIFDDLGWVDTFRVINHEPLQYTWWSNRGQARANNVGWRIDYHISTSALKDKVVPESD
HHHHHHHHCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEEEEHHHHHHCCCCCCC
YVYKENWFSDHAPLTINYDYEV
CEEECCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]