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The map label for this gene is trmE [H]

Identifier: 89256498

GI number: 89256498

Start: 1128805

End: 1130157

Strand: Reverse

Name: trmE [H]

Synonym: FTL_1177

Alternate gene names: 89256498

Gene position: 1130157-1128805 (Counterclockwise)

Preceding gene: 89256499

Following gene: 89256496

Centisome position: 59.61

GC content: 31.86

Gene sequence:

>1353_bases
ATGTACACAAAAGATACAATAGTTGCAATAGCAACTCCTCAAGGTAATGGCGGTATAGGCATAATCCGAATATCTGGGAT
AGATGCTCTAGAAATAGCTGAGAAACTAACTAAAAAACAGCTAAAACCTCGTTATGCAACATTCTGTAATGTCTATAATG
ATAATGAAATAATAGATCATGGTATCGTAATATTCTTTAAGGCGCCTTTGTCATATACAGGTGAAGACGTTGTAGAAATT
CAAGCTCATGGTAACCCGTTTATATTGAATTTGATTATCAAAGCAGCACTTAACTGTGGTGCCAGAATGGCAAAGGCAGG
TGAATTTACTGAGAGAGCATTTCTGAATAATAAACTTGATTTAGCTCAAGCAGAAGCTGTTGCTGATATTATTAATGCAT
CATCAGAAATAGCAGCTAAGTCGGCAGCTAAATCACTTCAAGGAGATTTCTCTAAAGAAATAAATAATCTTTTAGAAAAA
CTTATTTATCTAAGAATGTATGTAGAAGCGTCTATTGACTTTCCAGAAGAAGAAATAAACTTTTTAGAAGATCAAAAAAT
TCATTCTAGTTTAGAAGAAATATATAAGGTTATCTTAGCGATAAAAAATAGCTGTAAACAAGGTGTTATTCTTGCTGAGG
GAATTACATTAATATTAGTAGGCAAACCAAATGCTGGTAAGTCAAGCCTTTTGAATGCTCTTGCAGGTAAAGAATCAGCT
ATTGTAACATCAATAGCAGGTACAACTAGAGATATTGTCAAAGAACATATTCAAATAAATGGCGTTCCTATGCATATCAT
TGACACTGCAGGGCTACGTAATAGTGATGATATCATCGAAAGTGAAGGAATCAAAAGAGCTATTAAGAAAATTCAAGAAG
CTGATCAAGTACTATTTGTAACTGATGACTATACGAATAGCCAAGTTAAATTTAGTGATATAAAAGAGATAATTCCAGAA
TTTTATGATCAAATTCCTAAAGATATCGATATTACATATGTACATAATAAGATAGATCTCCTTAAAGAAGTTCCACACAA
TCATGCTAACCACATATACATATCAGCCGAAAATAACATTGGTATTGATAAACTAAAAGAACATATTCTCAATAAAGTTG
GTTATACAAATCAAAATGAAAGTATTTATACTGCACGTGAAAGACATGTTACAGCGATAAATAATGCTTTTGAGCATATT
AAACTTGCTAAAGAACAATTAGAACTTGGTAATGGTGAGCTTCTAGCTGAAGAGTTGTTAATTGTTCAAGAATATCTTAA
TTCAATTACAGGTGAATTTAGTTCTGATGACTTATTAGGTGAAATATTCTCAAGCTTTTGTATCGGCAAATAA

Upstream 100 bases:

>100_bases
ATCCAGAATTTTTTAGACAATATAAGAGTAAAAAAGGTAGAGTTATTGATCAAGAAAATAATGATTTTGATAGTTCAAAT
CAAAAGTTAAGATAGATAAT

Downstream 100 bases:

>100_bases
ATTGTCTAGCTATTTATTAGCAACTGCTATTTTATCTTCTAGTTGCTGAACCCTTGATGATTCTTGTTGTTCGTTATTTC
TAGCTTTTGACATGTTATTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MYTKDTIVAIATPQGNGGIGIIRISGIDALEIAEKLTKKQLKPRYATFCNVYNDNEIIDHGIVIFFKAPLSYTGEDVVEI
QAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLDLAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEK
LIYLRMYVEASIDFPEEEINFLEDQKIHSSLEEIYKVILAIKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFVTDDYTNSQVKFSDIKEIIPE
FYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNIGIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHI
KLAKEQLELGNGELLAEELLIVQEYLNSITGEFSSDDLLGEIFSSFCIGK

Sequences:

>Translated_450_residues
MYTKDTIVAIATPQGNGGIGIIRISGIDALEIAEKLTKKQLKPRYATFCNVYNDNEIIDHGIVIFFKAPLSYTGEDVVEI
QAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLDLAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEK
LIYLRMYVEASIDFPEEEINFLEDQKIHSSLEEIYKVILAIKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFVTDDYTNSQVKFSDIKEIIPE
FYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNIGIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHI
KLAKEQLELGNGELLAEELLIVQEYLNSITGEFSSDDLLGEIFSSFCIGK
>Mature_450_residues
MYTKDTIVAIATPQGNGGIGIIRISGIDALEIAEKLTKKQLKPRYATFCNVYNDNEIIDHGIVIFFKAPLSYTGEDVVEI
QAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLDLAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEK
LIYLRMYVEASIDFPEEEINFLEDQKIHSSLEEIYKVILAIKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFVTDDYTNSQVKFSDIKEIIPE
FYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNIGIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHI
KLAKEQLELGNGELLAEELLIVQEYLNSITGEFSSDDLLGEIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=469, Percent_Identity=31.5565031982942, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=29.6593186372746, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI193082993, Length=466, Percent_Identity=30.68669527897, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI2367268, Length=459, Percent_Identity=52.723311546841, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI87082120, Length=84, Percent_Identity=39.2857142857143, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=29.4372294372294, Blast_Score=175, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323665, Length=504, Percent_Identity=29.7619047619048, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI45550813, Length=475, Percent_Identity=30.3157894736842, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49841; Mature: 49841

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTKDTIVAIATPQGNGGIGIIRISGIDALEIAEKLTKKQLKPRYATFCNVYNDNEIIDH
CCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHEECCCCEEECC
GIVIFFKAPLSYTGEDVVEIQAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLD
CEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHCCCCH
LAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEKLIYLRMYVEASIDFPEEEIN
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FLEDQKIHSSLEEIYKVILAIKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCHHHHHHHHHCCCCC
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFV
EEEHHCCCHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
TDDYTNSQVKFSDIKEIIPEFYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNI
ECCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCC
GIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHIKLAKEQLELGNGELLAEELL
CHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IVQEYLNSITGEFSSDDLLGEIFSSFCIGK
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MYTKDTIVAIATPQGNGGIGIIRISGIDALEIAEKLTKKQLKPRYATFCNVYNDNEIIDH
CCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHEECCCCEEECC
GIVIFFKAPLSYTGEDVVEIQAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLD
CEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHCCCCH
LAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEKLIYLRMYVEASIDFPEEEIN
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FLEDQKIHSSLEEIYKVILAIKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCHHHHHHHHHCCCCC
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFV
EEEHHCCCHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
TDDYTNSQVKFSDIKEIIPEFYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNI
ECCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCC
GIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHIKLAKEQLELGNGELLAEELL
CHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IVQEYLNSITGEFSSDDLLGEIFSSFCIGK
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA