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The map label for this gene is rpoD [H]

Identifier: 89256388

GI number: 89256388

Start: 1005525

End: 1007258

Strand: Direct

Name: rpoD [H]

Synonym: FTL_1050

Alternate gene names: 89256388

Gene position: 1005525-1007258 (Clockwise)

Preceding gene: 89256387

Following gene: 89256389

Centisome position: 53.03

GC content: 32.18

Gene sequence:

>1734_bases
ATGACAAAAGAAGATTTGCTCTCTGATTTAAAAGATTTGATGATTGATGGTAGGGAAAGAGGATATTTAACTAGAGCCGA
TATTTTAGATGCTCTACCAGGAGATGTTTCAGAAGATCCTAAAATATATGAAGAAATTGAGGCTATACTTATTGACGCTG
GTATTGATGTCTATGATAGAACTCCTCAGATAGAAGAAGATGATGAAAGAAAGGTTAGTGAAGCTAATCTTGATGATTTA
AAAGGTAAAACTTCAGATCCTATTCGTATGTATATGCGCGAAATGGGTATTGTTGATCTTTTAGATAAAAAAGGCGAGAC
GGATATCGCTATTAGGATAGAAGAAGGTACTACAGAAGTTTTTAGTACGATTTTAAGCTATCCGATAGTAATTAAAACTT
ATATTGAGCGTTTCCGTGAGCTAGAAGAAAAAGCTATAGATTATATGAATTCCCAAGATATTGAGAATGAGCCTGTAGCT
AGATATATTCGTTTTAATGAAGTTATGGCTGGTTTTAGTGATGAACAGGTAACTGAAGAAAAGGTTGCTGAAGATGATCA
TGAGGAAAAAATTGATACTCAAAGGGCTTATGAATTCTTTACAAATTTAGAGAAAATGTTTGAAGAATATCAAGATAAGC
CTAACAAAAAACTTTATACTAAAATAGTTAAGGAATTTGATAATTTAAGATTGTCAACATCACATTTACAAAAGTTGGTT
GATTATATTAGATTACCTTATGCTCGAGTTAAAGAATTCGAAAGAAAAATTTTAAGACTCTGTGTTGAAAGATCAAAAAC
ACCACGCCAAGAGTTTATTAAAGTTTATAAAGTTGGTTCTCTAGAATGGTTGGAGCCATTAACTAAAAAGTATAAATTTA
CTGAGAATACTATTAGAGAAATAAAAAATTTAACTAAGCAAATTAATCAATTCCAAATTTTAATGATGATGGATATTGAA
GAGCTTAAACAAGTCAATCTTGAGATTTCAAGGAGTGAAGCTAAAATTACTCAAGCTAAAAAAGAGATGATAGAAGCAAA
CTTAAGACTAGTTGTTTCTGAAGCTAAAAAGTATACCAATAGAGGCTTACATTTCTTAGATATTATCCAAGAAGGAAATA
TTGGCTTAATGAAAGCTGTAGATAAGTTTGATTATCGTAAAGGCTTTAAGTTCTCGACATATGCTACTTGGTGGATACGT
CAAGCAATTACTCGTTCGATTGCAGACCAAGCTAGAACTATTCGTGTACCTGTACATATGATTGAGACAATCAATAAAGT
AAATAGAATTAAACGTCAGATTCTACAAGAAAAAGGGCGTGAAGCTACAGAAGAGGAGATTATCGAGCATACTCCTAATA
TGAGTAAAGAGAAGCTGAAAAAGATTCTTAATATTTCACATACACCAATTTCAATGGAGAGCCCAATTGGTGATGATGAA
GATTCTACAGTGGGCGATTTTATTGAGGATAAAAATAACTATTCTCCGATAGAGGCAGCTAATTTAGAAAATTTACGCGA
AGCAATAAAGGAGCTTATTGAAACTGGCTTAACTGAGAGAGAGGCTAAAGTTCTAATGATGCGTTTTGGTATTGGTATGA
ATACTGATCATACTTTAGAAGAAGTAGGTAAGCAGTTTAATGTTACTAGAGAACGTATTAGACAAATTGAGGCTAAGGCT
CTTAGAAAGCTTAAACATCCTTCTAGATCGGCATTTTTGAAAACATTTTTGTAA

Upstream 100 bases:

>100_bases
GCCCTTTAGGTCGGATGTCCAGGAAATGGAGCGTAAGTATTTAGTAGCAAAATTAGGCAACAGTAATATAATATAAAATA
AAATTCTATTGGAGTACTAG

Downstream 100 bases:

>100_bases
TTCAATATCTTAAATATTTTTTAAACTTTTCTATTTTAAATATTCTTCTTAGCTTATCTATAGCTATATATGTTGTTATA
ATGTTATAGTGAAGTAAGAG

Product: RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MTKEDLLSDLKDLMIDGRERGYLTRADILDALPGDVSEDPKIYEEIEAILIDAGIDVYDRTPQIEEDDERKVSEANLDDL
KGKTSDPIRMYMREMGIVDLLDKKGETDIAIRIEEGTTEVFSTILSYPIVIKTYIERFRELEEKAIDYMNSQDIENEPVA
RYIRFNEVMAGFSDEQVTEEKVAEDDHEEKIDTQRAYEFFTNLEKMFEEYQDKPNKKLYTKIVKEFDNLRLSTSHLQKLV
DYIRLPYARVKEFERKILRLCVERSKTPRQEFIKVYKVGSLEWLEPLTKKYKFTENTIREIKNLTKQINQFQILMMMDIE
ELKQVNLEISRSEAKITQAKKEMIEANLRLVVSEAKKYTNRGLHFLDIIQEGNIGLMKAVDKFDYRKGFKFSTYATWWIR
QAITRSIADQARTIRVPVHMIETINKVNRIKRQILQEKGREATEEEIIEHTPNMSKEKLKKILNISHTPISMESPIGDDE
DSTVGDFIEDKNNYSPIEAANLENLREAIKELIETGLTEREAKVLMMRFGIGMNTDHTLEEVGKQFNVTRERIRQIEAKA
LRKLKHPSRSAFLKTFL

Sequences:

>Translated_577_residues
MTKEDLLSDLKDLMIDGRERGYLTRADILDALPGDVSEDPKIYEEIEAILIDAGIDVYDRTPQIEEDDERKVSEANLDDL
KGKTSDPIRMYMREMGIVDLLDKKGETDIAIRIEEGTTEVFSTILSYPIVIKTYIERFRELEEKAIDYMNSQDIENEPVA
RYIRFNEVMAGFSDEQVTEEKVAEDDHEEKIDTQRAYEFFTNLEKMFEEYQDKPNKKLYTKIVKEFDNLRLSTSHLQKLV
DYIRLPYARVKEFERKILRLCVERSKTPRQEFIKVYKVGSLEWLEPLTKKYKFTENTIREIKNLTKQINQFQILMMMDIE
ELKQVNLEISRSEAKITQAKKEMIEANLRLVVSEAKKYTNRGLHFLDIIQEGNIGLMKAVDKFDYRKGFKFSTYATWWIR
QAITRSIADQARTIRVPVHMIETINKVNRIKRQILQEKGREATEEEIIEHTPNMSKEKLKKILNISHTPISMESPIGDDE
DSTVGDFIEDKNNYSPIEAANLENLREAIKELIETGLTEREAKVLMMRFGIGMNTDHTLEEVGKQFNVTRERIRQIEAKA
LRKLKHPSRSAFLKTFL
>Mature_576_residues
TKEDLLSDLKDLMIDGRERGYLTRADILDALPGDVSEDPKIYEEIEAILIDAGIDVYDRTPQIEEDDERKVSEANLDDLK
GKTSDPIRMYMREMGIVDLLDKKGETDIAIRIEEGTTEVFSTILSYPIVIKTYIERFRELEEKAIDYMNSQDIENEPVAR
YIRFNEVMAGFSDEQVTEEKVAEDDHEEKIDTQRAYEFFTNLEKMFEEYQDKPNKKLYTKIVKEFDNLRLSTSHLQKLVD
YIRLPYARVKEFERKILRLCVERSKTPRQEFIKVYKVGSLEWLEPLTKKYKFTENTIREIKNLTKQINQFQILMMMDIEE
LKQVNLEISRSEAKITQAKKEMIEANLRLVVSEAKKYTNRGLHFLDIIQEGNIGLMKAVDKFDYRKGFKFSTYATWWIRQ
AITRSIADQARTIRVPVHMIETINKVNRIKRQILQEKGREATEEEIIEHTPNMSKEKLKKILNISHTPISMESPIGDDED
STVGDFIEDKNNYSPIEAANLENLREAIKELIETGLTEREAKVLMMRFGIGMNTDHTLEEVGKQFNVTRERIRQIEAKAL
RKLKHPSRSAFLKTFL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=631, Percent_Identity=44.3740095087163, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1789098, Length=228, Percent_Identity=40.7894736842105, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1789871, Length=244, Percent_Identity=28.6885245901639, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788231, Length=200, Percent_Identity=28.5, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 67647; Mature: 67516

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00136 SUBTILASE_ASP ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEDLLSDLKDLMIDGRERGYLTRADILDALPGDVSEDPKIYEEIEAILIDAGIDVYDR
CCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
TPQIEEDDERKVSEANLDDLKGKTSDPIRMYMREMGIVDLLDKKGETDIAIRIEEGTTEV
CCCCCCCHHHHHHHCCHHHCCCCCCCHHHHHHHHHCHHHHHHCCCCCCEEEEEECCCHHH
FSTILSYPIVIKTYIERFRELEEKAIDYMNSQDIENEPVARYIRFNEVMAGFSDEQVTEE
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
KVAEDDHEEKIDTQRAYEFFTNLEKMFEEYQDKPNKKLYTKIVKEFDNLRLSTSHLQKLV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
DYIRLPYARVKEFERKILRLCVERSKTPRQEFIKVYKVGSLEWLEPLTKKYKFTENTIRE
HHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHH
IKNLTKQINQFQILMMMDIEELKQVNLEISRSEAKITQAKKEMIEANLRLVVSEAKKYTN
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLHFLDIIQEGNIGLMKAVDKFDYRKGFKFSTYATWWIRQAITRSIADQARTIRVPVHM
CCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHH
IETINKVNRIKRQILQEKGREATEEEIIEHTPNMSKEKLKKILNISHTPISMESPIGDDE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
DSTVGDFIEDKNNYSPIEAANLENLREAIKELIETGLTEREAKVLMMRFGIGMNTDHTLE
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHH
EVGKQFNVTRERIRQIEAKALRKLKHPSRSAFLKTFL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
TKEDLLSDLKDLMIDGRERGYLTRADILDALPGDVSEDPKIYEEIEAILIDAGIDVYDR
CHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
TPQIEEDDERKVSEANLDDLKGKTSDPIRMYMREMGIVDLLDKKGETDIAIRIEEGTTEV
CCCCCCCHHHHHHHCCHHHCCCCCCCHHHHHHHHHCHHHHHHCCCCCCEEEEEECCCHHH
FSTILSYPIVIKTYIERFRELEEKAIDYMNSQDIENEPVARYIRFNEVMAGFSDEQVTEE
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
KVAEDDHEEKIDTQRAYEFFTNLEKMFEEYQDKPNKKLYTKIVKEFDNLRLSTSHLQKLV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
DYIRLPYARVKEFERKILRLCVERSKTPRQEFIKVYKVGSLEWLEPLTKKYKFTENTIRE
HHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHH
IKNLTKQINQFQILMMMDIEELKQVNLEISRSEAKITQAKKEMIEANLRLVVSEAKKYTN
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLHFLDIIQEGNIGLMKAVDKFDYRKGFKFSTYATWWIRQAITRSIADQARTIRVPVHM
CCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHH
IETINKVNRIKRQILQEKGREATEEEIIEHTPNMSKEKLKKILNISHTPISMESPIGDDE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
DSTVGDFIEDKNNYSPIEAANLENLREAIKELIETGLTEREAKVLMMRFGIGMNTDHTLE
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHH
EVGKQFNVTRERIRQIEAKALRKLKHPSRSAFLKTFL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]