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The map label for this gene is cysN [H]

Identifier: 89256372

GI number: 89256372

Start: 992306

End: 993259

Strand: Direct

Name: cysN [H]

Synonym: FTL_1034

Alternate gene names: 89256372

Gene position: 992306-993259 (Clockwise)

Preceding gene: 89256371

Following gene: 89256373

Centisome position: 52.34

GC content: 33.23

Gene sequence:

>954_bases
ATGTCACATAAGTCTGATTTAATTGCAACAAATATCCAAAAGTACTTACAGCAACATCAACAAAAAGAATTATTAAGATT
TATCACATGTGGAAGTGTAGATGATGGTAAGAGTACATTAATAGGTAGGCTTTTACATGATAGTAAGTTAATTTTTGAAG
ATCAATTAGCTTCAATCGTAGCAGATAGTAAGAAAGTCGGTACTCAGGGTGATAGGTTAGATTTGGCACTGTTAGTTGAT
GGTTTACAATCTGAAAGAGAACAGGGTATTACTATTGATGTTGCTTATAGGTATTTCTCAACTGATAAACGAAAATTTAT
TATTGCAGATACTCCTGGTCATGAGCAGTATACTAGAAATATGGCTACAGGAGCTTCAAATTGTGATCTAGCTATTATAC
TTATAGATGCTAGAAAAGGACTACAAACACAAACGCGTAGACACAGTTTTATCTGTTCATTACTTGGTATAAAGCATGTC
ATAGTTGCTGTTAATAAGATGGATACTGTAGGCTACTCTCAACAAATTTATAAATCTATACAAGATGAATATTTGAAATT
AGCGGGAAGTTTACAAATTCCAGATATTAGATTTGTACCTATATCAGCTCTTGATGGTGATAATGTAGTTTCAAAAAGCC
CAAAAATGCCTTGGTTTAGAGGTTCTCCACTAATGCATTATTTAGAAACTATAAAAATAGATTATGCTTATACTGATGAG
TTTCGTTTTCCTGTACAGTTAGTTTGTAGACCAAATTCAGAGTTTAGAGGATTTCAGGGAACTGTAGTATCAGGTTCTGC
TAAAATAGGAGATACTATTAGGGTAATGCCTAGTGGTAAAATTACTACAATAAAGTCAATACTAAGCTTTGATGGAGAGT
TAAACGAAGCGGAGGCTGGTCAGTCTATAACTATAACTACCTACGATGAAATAGATATTAGTAGGGGGATATGA

Upstream 100 bases:

>100_bases
ATGATCTTACTTTATTATATTGTTATATTTAAAAGTCAAAACGTAGTAGATAATCTTTATTTAAGTTACGTTTATATTTT
ATTGACTAGGGATAATTTTA

Downstream 100 bases:

>100_bases
TTGTTCATAAAGATGCGATATCACATGTATCATCAATGCTTAGAGCAAATATTGTATGGATGTCTGAACAACCTTTAATA
GAGTATAAAGATTATTATAT

Product: sulfate adenylate transferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSHKSDLIATNIQKYLQQHQQKELLRFITCGSVDDGKSTLIGRLLHDSKLIFEDQLASIVADSKKVGTQGDRLDLALLVD
GLQSEREQGITIDVAYRYFSTDKRKFIIADTPGHEQYTRNMATGASNCDLAIILIDARKGLQTQTRRHSFICSLLGIKHV
IVAVNKMDTVGYSQQIYKSIQDEYLKLAGSLQIPDIRFVPISALDGDNVVSKSPKMPWFRGSPLMHYLETIKIDYAYTDE
FRFPVQLVCRPNSEFRGFQGTVVSGSAKIGDTIRVMPSGKITTIKSILSFDGELNEAEAGQSITITTYDEIDISRGI

Sequences:

>Translated_317_residues
MSHKSDLIATNIQKYLQQHQQKELLRFITCGSVDDGKSTLIGRLLHDSKLIFEDQLASIVADSKKVGTQGDRLDLALLVD
GLQSEREQGITIDVAYRYFSTDKRKFIIADTPGHEQYTRNMATGASNCDLAIILIDARKGLQTQTRRHSFICSLLGIKHV
IVAVNKMDTVGYSQQIYKSIQDEYLKLAGSLQIPDIRFVPISALDGDNVVSKSPKMPWFRGSPLMHYLETIKIDYAYTDE
FRFPVQLVCRPNSEFRGFQGTVVSGSAKIGDTIRVMPSGKITTIKSILSFDGELNEAEAGQSITITTYDEIDISRGI
>Mature_316_residues
SHKSDLIATNIQKYLQQHQQKELLRFITCGSVDDGKSTLIGRLLHDSKLIFEDQLASIVADSKKVGTQGDRLDLALLVDG
LQSEREQGITIDVAYRYFSTDKRKFIIADTPGHEQYTRNMATGASNCDLAIILIDARKGLQTQTRRHSFICSLLGIKHVI
VAVNKMDTVGYSQQIYKSIQDEYLKLAGSLQIPDIRFVPISALDGDNVVSKSPKMPWFRGSPLMHYLETIKIDYAYTDEF
RFPVQLVCRPNSEFRGFQGTVVSGSAKIGDTIRVMPSGKITTIKSILSFDGELNEAEAGQSITITTYDEIDISRGI

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=323, Percent_Identity=31.5789473684211, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI5729864, Length=323, Percent_Identity=31.5789473684211, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI194018520, Length=303, Percent_Identity=33.003300330033, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI194097354, Length=303, Percent_Identity=33.003300330033, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI194018522, Length=303, Percent_Identity=33.003300330033, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI46094014, Length=303, Percent_Identity=32.6732673267327, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4503475, Length=324, Percent_Identity=31.7901234567901, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI4503471, Length=325, Percent_Identity=32, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI34147630, Length=301, Percent_Identity=27.5747508305648, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1789108, Length=309, Percent_Identity=62.7831715210356, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI2367247, Length=296, Percent_Identity=24.3243243243243, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790412, Length=140, Percent_Identity=32.1428571428571, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789737, Length=140, Percent_Identity=32.1428571428571, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566629, Length=326, Percent_Identity=30.6748466257669, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=282, Percent_Identity=30.4964539007092, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=282, Percent_Identity=30.1418439716312, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17552884, Length=324, Percent_Identity=30.5555555555556, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17569207, Length=324, Percent_Identity=30.5555555555556, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=317, Percent_Identity=28.391167192429, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=31.5436241610738, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=227, Percent_Identity=26.8722466960352, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=151, Percent_Identity=31.7880794701987, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322937, Length=313, Percent_Identity=30.3514376996805, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6325337, Length=307, Percent_Identity=29.6416938110749, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319594, Length=307, Percent_Identity=29.6416938110749, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320377, Length=314, Percent_Identity=31.8471337579618, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324761, Length=290, Percent_Identity=28.6206896551724, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=207, Percent_Identity=29.4685990338164, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17137380, Length=318, Percent_Identity=30.188679245283, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45553807, Length=309, Percent_Identity=32.3624595469256, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553816, Length=309, Percent_Identity=32.3624595469256, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24651721, Length=309, Percent_Identity=32.3624595469256, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17864154, Length=309, Percent_Identity=32.3624595469256, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24652838, Length=309, Percent_Identity=32.0388349514563, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137572, Length=309, Percent_Identity=32.0388349514563, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45550900, Length=304, Percent_Identity=30.9210526315789, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI281363316, Length=326, Percent_Identity=26.6871165644172, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17864358, Length=326, Percent_Identity=26.6871165644172, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921738, Length=159, Percent_Identity=31.4465408805031, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922690, Length=301, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35390; Mature: 35259

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKSDLIATNIQKYLQQHQQKELLRFITCGSVDDGKSTLIGRLLHDSKLIFEDQLASIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
ADSKKVGTQGDRLDLALLVDGLQSEREQGITIDVAYRYFSTDKRKFIIADTPGHEQYTRN
HCCHHCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHH
MATGASNCDLAIILIDARKGLQTQTRRHSFICSLLGIKHVIVAVNKMDTVGYSQQIYKSI
HHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEHEEECCCCCCHHHHHHHHH
QDEYLKLAGSLQIPDIRFVPISALDGDNVVSKSPKMPWFRGSPLMHYLETIKIDYAYTDE
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEEEECC
FRFPVQLVCRPNSEFRGFQGTVVSGSAKIGDTIRVMPSGKITTIKSILSFDGELNEAEAG
CCCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHCCC
QSITITTYDEIDISRGI
CEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SHKSDLIATNIQKYLQQHQQKELLRFITCGSVDDGKSTLIGRLLHDSKLIFEDQLASIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
ADSKKVGTQGDRLDLALLVDGLQSEREQGITIDVAYRYFSTDKRKFIIADTPGHEQYTRN
HCCHHCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHH
MATGASNCDLAIILIDARKGLQTQTRRHSFICSLLGIKHVIVAVNKMDTVGYSQQIYKSI
HHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEHEEECCCCCCHHHHHHHHH
QDEYLKLAGSLQIPDIRFVPISALDGDNVVSKSPKMPWFRGSPLMHYLETIKIDYAYTDE
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEEEECC
FRFPVQLVCRPNSEFRGFQGTVVSGSAKIGDTIRVMPSGKITTIKSILSFDGELNEAEAG
CCCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHCCC
QSITITTYDEIDISRGI
CEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA