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The map label for this gene is miaB [H]

Identifier: 89256244

GI number: 89256244

Start: 864174

End: 865502

Strand: Reverse

Name: miaB [H]

Synonym: FTL_0886

Alternate gene names: 89256244

Gene position: 865502-864174 (Counterclockwise)

Preceding gene: 89256257

Following gene: 89256243

Centisome position: 45.65

GC content: 35.36

Gene sequence:

>1329_bases
ATGAAAGAACAAAAAAAGGTTTTCATAAAAACTTTAGGTTGCCAAATGAATGAGTATGACTCTGCTAGAATGCATGAGGT
TCTAAATGAGCATTTTGATACTGTAAAGACAGATGATTATAAAGATGCAGATATCATCCTTATTAATACTTGCTCTATCA
GAGAAAAAGCTCAAGAAAAAGTATTCCACGAGCTTGGAAGATGGAAAGGCATAAAAAAAACTAATGAAGATCTAATAATC
GGTGTTGGTGGTTGTGTTGCTTCACAAGAAGGTGAAAATATTATCAAAAGAGCTCCTTTTGTTGACCTAGTATTTGGTCC
GCAAACTATTCATAGACTTCCAGAGATGATCAAGCAAAAGCAAAAATCTCAACAATCTCAAGTTGATATATCATTTCCAG
AGGTTGAAAAATTTGATTATCTACCAGAGCCAAAAGCTGAAGGCGCTAAAGCATATGTATCTATTATGGAAGGTTGTGAT
AAGTACTGCTCATACTGTGTAGTACCATATACGCGTGGTCCCGAAGTAAATAGACCATTTGAGGATGTCTTAGCAGAATG
TGCTATATTAGCAGAACAAGGTGTCAAAGAAATTACCCTACTTGGACAAAATGTCAATCATTACTTAGGTCCAATGGAAA
ATGGTCAAACAGCAGATCTTGCTCTACTAATCCACTTTATCGCTGAGATAGATGGGATTGAGAGAATTAGGTTCACTACT
TCACATCCGGTTGAGTTTTCACAAAACCTTATCGATGCTTATGCAACGGTCCCAGAGCTTGCTAACCATTTACATTTACC
AGTACAACATGGTTCAGATAGAATTCTGATAAATATGAAAAGAAACCATACTATTTTAGAGTTTAAACAAAAAATTAGAA
AATTAAGAGCGATTCGCCCTGATATTACAATTTCATCTGATTTTATCGTCGGTTTTCCTGGTGAGACAGAAGAAGATTTC
CAAAAACTACTAGATTTAGTAAAAGAGATTAACTTTGACCAGTCATTTAGTTTCATCTATAGCAAGCGTCCTGGTACTCC
AGCGGCTGATTTACCAGATGATACGCCTATGGAAGTTAAAAAAGATCGACTTAAACGTCTACAGGATTTACTAAATAGTA
ACGCTCAAATAATCTCTAGACAGATGGTTGGCACTAACCAAAGAATTCTAGTTGATGGAACATCTAAAAAAGATGATAAC
ATCCTCTCTGGAAGAACAGAAAACAACAGAGTTGTTAACTTTAAGGGTGATAAATCTTTGATTGGTCAGTTCGCTATGGT
TAAAATAACTGAAAGCCTTCCAAACTCTCTTAGAGGAGAACTTATTTAA

Upstream 100 bases:

>100_bases
TAATGAAATCGTGCCAAGATTAGCACAGGTCGGTTAAGTGTATTTTTACATTAATTAATAGCGCTTTAGCTATACTACAA
AAATAAAGGGTTTCGATTAA

Downstream 100 bases:

>100_bases
CCTATGAATAAAACTCAATTTATTCTTGAACCTTATAACTACGATGCAATGATGCTACTTTGTGGCAACCTTGATGAAAA
TATTCGTGCTGTCGAAAATT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKEQKKVFIKTLGCQMNEYDSARMHEVLNEHFDTVKTDDYKDADIILINTCSIREKAQEKVFHELGRWKGIKKTNEDLII
GVGGCVASQEGENIIKRAPFVDLVFGPQTIHRLPEMIKQKQKSQQSQVDISFPEVEKFDYLPEPKAEGAKAYVSIMEGCD
KYCSYCVVPYTRGPEVNRPFEDVLAECAILAEQGVKEITLLGQNVNHYLGPMENGQTADLALLIHFIAEIDGIERIRFTT
SHPVEFSQNLIDAYATVPELANHLHLPVQHGSDRILINMKRNHTILEFKQKIRKLRAIRPDITISSDFIVGFPGETEEDF
QKLLDLVKEINFDQSFSFIYSKRPGTPAADLPDDTPMEVKKDRLKRLQDLLNSNAQIISRQMVGTNQRILVDGTSKKDDN
ILSGRTENNRVVNFKGDKSLIGQFAMVKITESLPNSLRGELI

Sequences:

>Translated_442_residues
MKEQKKVFIKTLGCQMNEYDSARMHEVLNEHFDTVKTDDYKDADIILINTCSIREKAQEKVFHELGRWKGIKKTNEDLII
GVGGCVASQEGENIIKRAPFVDLVFGPQTIHRLPEMIKQKQKSQQSQVDISFPEVEKFDYLPEPKAEGAKAYVSIMEGCD
KYCSYCVVPYTRGPEVNRPFEDVLAECAILAEQGVKEITLLGQNVNHYLGPMENGQTADLALLIHFIAEIDGIERIRFTT
SHPVEFSQNLIDAYATVPELANHLHLPVQHGSDRILINMKRNHTILEFKQKIRKLRAIRPDITISSDFIVGFPGETEEDF
QKLLDLVKEINFDQSFSFIYSKRPGTPAADLPDDTPMEVKKDRLKRLQDLLNSNAQIISRQMVGTNQRILVDGTSKKDDN
ILSGRTENNRVVNFKGDKSLIGQFAMVKITESLPNSLRGELI
>Mature_442_residues
MKEQKKVFIKTLGCQMNEYDSARMHEVLNEHFDTVKTDDYKDADIILINTCSIREKAQEKVFHELGRWKGIKKTNEDLII
GVGGCVASQEGENIIKRAPFVDLVFGPQTIHRLPEMIKQKQKSQQSQVDISFPEVEKFDYLPEPKAEGAKAYVSIMEGCD
KYCSYCVVPYTRGPEVNRPFEDVLAECAILAEQGVKEITLLGQNVNHYLGPMENGQTADLALLIHFIAEIDGIERIRFTT
SHPVEFSQNLIDAYATVPELANHLHLPVQHGSDRILINMKRNHTILEFKQKIRKLRAIRPDITISSDFIVGFPGETEEDF
QKLLDLVKEINFDQSFSFIYSKRPGTPAADLPDDTPMEVKKDRLKRLQDLLNSNAQIISRQMVGTNQRILVDGTSKKDDN
ILSGRTENNRVVNFKGDKSLIGQFAMVKITESLPNSLRGELI

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=34.6473029045643, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=34.6473029045643, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI93277076, Length=447, Percent_Identity=27.0693512304251, Blast_Score=152, Evalue=7e-37,
Organism=Escherichia coli, GI1786882, Length=438, Percent_Identity=63.013698630137, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1787057, Length=401, Percent_Identity=31.1720698254364, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=33.0490405117271, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=321, Percent_Identity=27.4143302180685, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=32.2105263157895, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI19922432, Length=460, Percent_Identity=28.2608695652174, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50165; Mature: 50165

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQKKVFIKTLGCQMNEYDSARMHEVLNEHFDTVKTDDYKDADIILINTCSIREKAQEK
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHH
VFHELGRWKGIKKTNEDLIIGVGGCVASQEGENIIKRAPFVDLVFGPQTIHRLPEMIKQK
HHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHH
QKSQQSQVDISFPEVEKFDYLPEPKAEGAKAYVSIMEGCDKYCSYCVVPYTRGPEVNRPF
HHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCH
EDVLAECAILAEQGVKEITLLGQNVNHYLGPMENGQTADLALLIHFIAEIDGIERIRFTT
HHHHHHHHHHHHCCCCEEEHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECC
SHPVEFSQNLIDAYATVPELANHLHLPVQHGSDRILINMKRNHTILEFKQKIRKLRAIRP
CCCHHHHHHHHHHHHHHHHHHHHEECEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCC
DITISSDFIVGFPGETEEDFQKLLDLVKEINFDQSFSFIYSKRPGTPAADLPDDTPMEVK
CCEECCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCHHHH
KDRLKRLQDLLNSNAQIISRQMVGTNQRILVDGTSKKDDNILSGRTENNRVVNFKGDKSL
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHH
IGQFAMVKITESLPNSLRGELI
HHHHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure
MKEQKKVFIKTLGCQMNEYDSARMHEVLNEHFDTVKTDDYKDADIILINTCSIREKAQEK
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHH
VFHELGRWKGIKKTNEDLIIGVGGCVASQEGENIIKRAPFVDLVFGPQTIHRLPEMIKQK
HHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHH
QKSQQSQVDISFPEVEKFDYLPEPKAEGAKAYVSIMEGCDKYCSYCVVPYTRGPEVNRPF
HHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCH
EDVLAECAILAEQGVKEITLLGQNVNHYLGPMENGQTADLALLIHFIAEIDGIERIRFTT
HHHHHHHHHHHHCCCCEEEHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECC
SHPVEFSQNLIDAYATVPELANHLHLPVQHGSDRILINMKRNHTILEFKQKIRKLRAIRP
CCCHHHHHHHHHHHHHHHHHHHHEECEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCC
DITISSDFIVGFPGETEEDFQKLLDLVKEINFDQSFSFIYSKRPGTPAADLPDDTPMEVK
CCEECCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCHHHH
KDRLKRLQDLLNSNAQIISRQMVGTNQRILVDGTSKKDDNILSGRTENNRVVNFKGDKSL
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHH
IGQFAMVKITESLPNSLRGELI
HHHHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA