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The map label for this gene is gcvA [H]

Identifier: 89256204

GI number: 89256204

Start: 825879

End: 826484

Strand: Reverse

Name: gcvA [H]

Synonym: FTL_0844

Alternate gene names: 89256204

Gene position: 826484-825879 (Counterclockwise)

Preceding gene: 89256217

Following gene: 89256202

Centisome position: 43.59

GC content: 28.88

Gene sequence:

>606_bases
ATGAAAACCAAAAGCTACACAAAAGCAGCTGAGCAACTATTTATGACTCATTCTGCAGTGAGCCAATCGATAAGAAAACT
AGAAGCCTATCTTGATAAAAAACTTTTTATAACTGACAAAAGAAAGCTTATTATCACTGATTTAGCTAGAGAGTATTATG
CAAAAATAGAGCCTTTAATAGCACAAATTCATTATTCAACTGAGGCTTTTAAAAAACAAAAATCCAAGAAGTTATCGATA
AACTGCATGACTACACTATGTGCAAACTGGTTAATTCCAAAACTTGATGAATTGATAGAATTTCTAGCAGACACAGAAAT
ACAACTTATAACACTAGGAAGAAAAGCAAACTTTGATTATGATGATGTAGATATTAGTATAGAGTATGGCATCGATAGCG
ACTTTAATAATAAAAACAAATACAAACTTGCTGAAGGTGAATTAATATTAGTTTGCAATAACAGACATACAGGTAAAAGC
TGTTTAGAGATAATATCACAACAAAAACTAATTTATGTAGATGATAAGATTCGTATTGATGATTATCTTCTTTGGCAACA
ACATAACAATATTTTAGCTAAAGTTAATAAAAAAAATTGTTTTTAA

Upstream 100 bases:

>100_bases
ACGTATTATTCTTACAACATAAGTAAGTTTTACTTACACATTAATAATAAAAAGAAACGATCTACCTCCTCTAAATTCCT
TACCAGTATTTATAGCTGTG

Downstream 100 bases:

>100_bases
AAATTCGCTACAAGCTATTAAGGCTTGTTTTTCAGGTATTGGATTCTTTGTTACTGACAAGCTTCTAATACAAGAATATA
TTAAAAATGGTTTCCTATAT

Product: LysR transcriptional regulator family protein

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKRKLIITDLAREYYAKIEPLIAQIHYSTEAFKKQKSKKLSI
NCMTTLCANWLIPKLDELIEFLADTEIQLITLGRKANFDYDDVDISIEYGIDSDFNNKNKYKLAEGELILVCNNRHTGKS
CLEIISQQKLIYVDDKIRIDDYLLWQQHNNILAKVNKKNCF

Sequences:

>Translated_201_residues
MKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKRKLIITDLAREYYAKIEPLIAQIHYSTEAFKKQKSKKLSI
NCMTTLCANWLIPKLDELIEFLADTEIQLITLGRKANFDYDDVDISIEYGIDSDFNNKNKYKLAEGELILVCNNRHTGKS
CLEIISQQKLIYVDDKIRIDDYLLWQQHNNILAKVNKKNCF
>Mature_201_residues
MKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKRKLIITDLAREYYAKIEPLIAQIHYSTEAFKKQKSKKLSI
NCMTTLCANWLIPKLDELIEFLADTEIQLITLGRKANFDYDDVDISIEYGIDSDFNNKNKYKLAEGELILVCNNRHTGKS
CLEIISQQKLIYVDDKIRIDDYLLWQQHNNILAKVNKKNCF

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=150, Percent_Identity=29.3333333333333, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1786448, Length=154, Percent_Identity=26.6233766233766, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 23454; Mature: 23454

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKRKLIITDLAREYYAKIEPLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEHHHHHHHHHHHHHHHHH
AQIHYSTEAFKKQKSKKLSINCMTTLCANWLIPKLDELIEFLADTEIQLITLGRKANFDY
HHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCC
DDVDISIEYGIDSDFNNKNKYKLAEGELILVCNNRHTGKSCLEIISQQKLIYVDDKIRID
CCEEEEEEECCCCCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEECCCEEEC
DYLLWQQHNNILAKVNKKNCF
CEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKRKLIITDLAREYYAKIEPLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEHHHHHHHHHHHHHHHHH
AQIHYSTEAFKKQKSKKLSINCMTTLCANWLIPKLDELIEFLADTEIQLITLGRKANFDY
HHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCC
DDVDISIEYGIDSDFNNKNKYKLAEGELILVCNNRHTGKSCLEIISQQKLIYVDDKIRID
CCEEEEEEECCCCCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEECCCEEEC
DYLLWQQHNNILAKVNKKNCF
CEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]