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The map label for this gene is tgt [H]

Identifier: 89256203

GI number: 89256203

Start: 824555

End: 825658

Strand: Direct

Name: tgt [H]

Synonym: FTL_0843

Alternate gene names: 89256203

Gene position: 824555-825658 (Clockwise)

Preceding gene: 89256201

Following gene: 89256205

Centisome position: 43.49

GC content: 37.14

Gene sequence:

>1104_bases
ATGACTGTAATGAAGTTTGATCTAATCAAAAAAGAGGGTAAAGCCCGCAGAGGTAAGATTACTTTTCCTCGTGGTGATAT
TCAAACACCTGCATTTATGCCAGTAGGTACTTATGGTGCTGTCAAATCACTATCTCCAGTTGAGTTAAAAGAGATGGGGG
CAGAGATTATTTTGGGTAATACATTTCATTTATGGTTACGTCCTGGCACAGAGATTATCAAAAAACATGGTTCACTCCAT
GGCTTTAACGGCTGGGATAAGCCTATTTTGACAGATTCTGGAGGTTTTCAGGTTTTTAGTTTGGGCAAGATGCGTAAGCT
AACAGAAGAGGGTGTGACATTTAAATCACCTGTTAATAGTTCTAAGGTATTTTTATCACCTGAAATATCAATGCAAGTGC
AAAGAGATTTAGGCTCAGATATCGTGATGTGTTTTGATGAATGTACGCCATACCCAGCTACGGAAAAAGAAGCTAAAGAA
TCTATGGAGCTATCTATGCGCTGGGCAAAAAGATCAAAAGAAGCTCATGGTGATAATCCATCGGCATTATTTGGGATTAT
TCAAGGTGGTATGTATGAGCACTTACGTGATGAGTCGTTGGCAAAGTTAAAAGAGATTGATTTTGATGGTTTTGCTATTG
GTGGCTTATCTGTTGGCGAGCCTAAAGAAGATATGATTAGAATACTTGATCATACCGCACATCAAATGCCAGAAGATAAA
CCAAGATATCTGATGGGAGTTGGCACTCCAAAAGATCTAGTTGAGGCGGTGTATCGTGGAGTAGATATGTTTGATTGTGT
TATGCCATCTCGAAATGCTCGCAATGGTCATATCTTCACTTCAGAAGGGGTGATTAAAATCAGAAACTCTAAGTATAAAG
ATGATACTTCACCATTAGATCCAAACTGTGATTGCTATACTTGTAAAAATTTTACTAAGAGCTATTTGCATCATCTTGAT
AAAACCAAAGAGATTCTAGGCTCTAGGTTAAATACTATTCATAATTTAACTTTCTACCAAAATCTTATGAAATCTATCCG
TAAGGCACTAGACGAAGGTAGATTTTCAGAGTTTAGAAAAGAGTTTTTAGCAAGCTATAAATAA

Upstream 100 bases:

>100_bases
CAGTTTTTTGATCCTGTGATTAAGCCATAGGGCGACGGAGAGGATTTAAAGTGTGTTGGGTTATCTATACTAATTATCTA
TTTTTATAAGGTTAATTATT

Downstream 100 bases:

>100_bases
GTCTAACTACTTGAAATAAGAAGTCAGTAATCCTTTAATTTTATCAAAATTTTCAGTTTCTGAGTCTTTTGCTAAAAGGT
AATATGATTTTCCTGTTGAG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MTVMKFDLIKKEGKARRGKITFPRGDIQTPAFMPVGTYGAVKSLSPVELKEMGAEIILGNTFHLWLRPGTEIIKKHGSLH
GFNGWDKPILTDSGGFQVFSLGKMRKLTEEGVTFKSPVNSSKVFLSPEISMQVQRDLGSDIVMCFDECTPYPATEKEAKE
SMELSMRWAKRSKEAHGDNPSALFGIIQGGMYEHLRDESLAKLKEIDFDGFAIGGLSVGEPKEDMIRILDHTAHQMPEDK
PRYLMGVGTPKDLVEAVYRGVDMFDCVMPSRNARNGHIFTSEGVIKIRNSKYKDDTSPLDPNCDCYTCKNFTKSYLHHLD
KTKEILGSRLNTIHNLTFYQNLMKSIRKALDEGRFSEFRKEFLASYK

Sequences:

>Translated_367_residues
MTVMKFDLIKKEGKARRGKITFPRGDIQTPAFMPVGTYGAVKSLSPVELKEMGAEIILGNTFHLWLRPGTEIIKKHGSLH
GFNGWDKPILTDSGGFQVFSLGKMRKLTEEGVTFKSPVNSSKVFLSPEISMQVQRDLGSDIVMCFDECTPYPATEKEAKE
SMELSMRWAKRSKEAHGDNPSALFGIIQGGMYEHLRDESLAKLKEIDFDGFAIGGLSVGEPKEDMIRILDHTAHQMPEDK
PRYLMGVGTPKDLVEAVYRGVDMFDCVMPSRNARNGHIFTSEGVIKIRNSKYKDDTSPLDPNCDCYTCKNFTKSYLHHLD
KTKEILGSRLNTIHNLTFYQNLMKSIRKALDEGRFSEFRKEFLASYK
>Mature_366_residues
TVMKFDLIKKEGKARRGKITFPRGDIQTPAFMPVGTYGAVKSLSPVELKEMGAEIILGNTFHLWLRPGTEIIKKHGSLHG
FNGWDKPILTDSGGFQVFSLGKMRKLTEEGVTFKSPVNSSKVFLSPEISMQVQRDLGSDIVMCFDECTPYPATEKEAKES
MELSMRWAKRSKEAHGDNPSALFGIIQGGMYEHLRDESLAKLKEIDFDGFAIGGLSVGEPKEDMIRILDHTAHQMPEDKP
RYLMGVGTPKDLVEAVYRGVDMFDCVMPSRNARNGHIFTSEGVIKIRNSKYKDDTSPLDPNCDCYTCKNFTKSYLHHLDK
TKEILGSRLNTIHNLTFYQNLMKSIRKALDEGRFSEFRKEFLASYK

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=41.4835164835165, Blast_Score=310, Evalue=9e-85,
Organism=Homo sapiens, GI13375872, Length=282, Percent_Identity=26.5957446808511, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786607, Length=361, Percent_Identity=64.819944598338, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17544680, Length=364, Percent_Identity=41.2087912087912, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=138, Percent_Identity=36.231884057971, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19920510, Length=354, Percent_Identity=45.4802259887006, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI21355311, Length=246, Percent_Identity=29.2682926829268, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41484; Mature: 41353

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVMKFDLIKKEGKARRGKITFPRGDIQTPAFMPVGTYGAVKSLSPVELKEMGAEIILGN
CCEEEHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCHHHCCCCCHHHHHCCCEEEECC
TFHLWLRPGTEIIKKHGSLHGFNGWDKPILTDSGGFQVFSLGKMRKLTEEGVTFKSPVNS
EEEEEECCCHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHCCCEEECCCCC
SKVFLSPEISMQVQRDLGSDIVMCFDECTPYPATEKEAKESMELSMRWAKRSKEAHGDNP
CEEEECCCHHHHHHHHCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
SALFGIIQGGMYEHLRDESLAKLKEIDFDGFAIGGLSVGEPKEDMIRILDHTAHQMPEDK
HHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
PRYLMGVGTPKDLVEAVYRGVDMFDCVMPSRNARNGHIFTSEGVIKIRNSKYKDDTSPLD
CCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCC
PNCDCYTCKNFTKSYLHHLDKTKEILGSRLNTIHNLTFYQNLMKSIRKALDEGRFSEFRK
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
EFLASYK
HHHHCCC
>Mature Secondary Structure 
TVMKFDLIKKEGKARRGKITFPRGDIQTPAFMPVGTYGAVKSLSPVELKEMGAEIILGN
CEEEHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCHHHCCCCCHHHHHCCCEEEECC
TFHLWLRPGTEIIKKHGSLHGFNGWDKPILTDSGGFQVFSLGKMRKLTEEGVTFKSPVNS
EEEEEECCCHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHCCCEEECCCCC
SKVFLSPEISMQVQRDLGSDIVMCFDECTPYPATEKEAKESMELSMRWAKRSKEAHGDNP
CEEEECCCHHHHHHHHCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
SALFGIIQGGMYEHLRDESLAKLKEIDFDGFAIGGLSVGEPKEDMIRILDHTAHQMPEDK
HHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
PRYLMGVGTPKDLVEAVYRGVDMFDCVMPSRNARNGHIFTSEGVIKIRNSKYKDDTSPLD
CCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCC
PNCDCYTCKNFTKSYLHHLDKTKEILGSRLNTIHNLTFYQNLMKSIRKALDEGRFSEFRK
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
EFLASYK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA