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The map label for this gene is proP [H]

Identifier: 89256135

GI number: 89256135

Start: 738935

End: 740212

Strand: Direct

Name: proP [H]

Synonym: FTL_0748

Alternate gene names: 89256135

Gene position: 738935-740212 (Clockwise)

Preceding gene: 89256134

Following gene: 89256136

Centisome position: 38.97

GC content: 33.18

Gene sequence:

>1278_bases
ATGTATGATAGAGATAGTAGAAATGTTATATTACTTGCCGGGATTGGTGCAATCCTTGAGTTTTATGACTTTGTTTTATA
TATGATTTTTTCTAAAGAAATTTCAGCAACATTTTTTGCACAAATCACTAACCCAACAATTAAAGCCTTTTTAACAGTTT
TGATATTTTCAATAGCATATCTGGTAAGACCATTTGCAGGAACTATACTTGGGATTATTGGTGACTTGATAGGACGTAGG
CGTCTTTTGTTATTTACGATATTACTTATGGGTGGATGTTCTTTATGTATGGGATTGATGCCTGGTTATGCTCAATGGGG
CTTATTTGCTAGTTTTGCTTTTGTTGTTTTACGTATAATGCAAGGTATAGCTTTAGGCGGTGAACTACCAGGTGCGTATG
TGATCGTTTATGAATCTGTCAAAGGTAAAATAGGTTTTGCTACAGCGATTTTATTTACATTTGTAACTGTAGGTTTTTTA
TTCTCTGATTTTGTTGGTTTTACTCTTGAATATATTTTTGGTGATTTTGCTTGGCGAGCAGGATTTATCATTGGCGGACT
TTTGGGTTTTGTTGGCTATTATGTGCGTCGTAACCTTAATGAAACTCGAGAATTTGAGAATATTGACAAGCAAAAAAGAC
ATTCCTTTAGTTCTTTGATATCAACTTATGGAGCAAATTTATTTGCAGGAATATGTATGGTTATTATAGTAGCATTTGGC
GGGGTAATGCTTACTCTTTATATCCATAAGTTTGTTGAAGATATTTTAGTCAGTTATAATTCTGGACAAATCTCTTTAAT
TCTAATGCCAAGTGTTTTAACACTTACTTGTTTTACTTTTGTATTTGGTTTTTTATCAGATAAAGTTGGCATTGCAAAAA
TGTTTACACTAGGAGCTGGATTTATTGTAGTTTGTGCTTTGCCAGTATTCTACTTGATGAGTAGCATTGGTAGTGTCTCT
GCTATAGTTATTACTTCAGTGATAATAATGCTTTGCTATGCTTTAGTTGCTGCTACATTTATATTTTTACTTTGTGATCT
TTTTCCTACTGATGTTAGATTATCGGGTGTAGGACTGAGCTATAATCTTGCTTTTGCTATAGTTGGTGGAGTTGCTCCTT
TGCTTAGTACAACGATTATCACAATGACAGCTTATAATTTCTTAGGTCCAGCTATTGTTGGTATAGTTTGTGGAGTGCTT
GGTCTATTAGGAATGTTTATCTATCTTAGAAAAGGTGGCTATCATAAAACTAACAAAGACATGATAGTTAAACTATAA

Upstream 100 bases:

>100_bases
TAGCTACTAAAAATTATTAATACTTGTATAAAGGTTTCTTTTAAATGAAACTAAAAACTTGTATGATTTGCTAAACTAGT
TATTAGAGCGAGTATTGCAA

Downstream 100 bases:

>100_bases
CTAACAAAGGAATTCTGATAAATAGCCAATAATTTTACTAGGTTCTGTGCACAAAAAACTTAAATTATATTGAAAATAGC
TAAACTGCTGTTATTTAAGA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MYDRDSRNVILLAGIGAILEFYDFVLYMIFSKEISATFFAQITNPTIKAFLTVLIFSIAYLVRPFAGTILGIIGDLIGRR
RLLLFTILLMGGCSLCMGLMPGYAQWGLFASFAFVVLRIMQGIALGGELPGAYVIVYESVKGKIGFATAILFTFVTVGFL
FSDFVGFTLEYIFGDFAWRAGFIIGGLLGFVGYYVRRNLNETREFENIDKQKRHSFSSLISTYGANLFAGICMVIIVAFG
GVMLTLYIHKFVEDILVSYNSGQISLILMPSVLTLTCFTFVFGFLSDKVGIAKMFTLGAGFIVVCALPVFYLMSSIGSVS
AIVITSVIIMLCYALVAATFIFLLCDLFPTDVRLSGVGLSYNLAFAIVGGVAPLLSTTIITMTAYNFLGPAIVGIVCGVL
GLLGMFIYLRKGGYHKTNKDMIVKL

Sequences:

>Translated_425_residues
MYDRDSRNVILLAGIGAILEFYDFVLYMIFSKEISATFFAQITNPTIKAFLTVLIFSIAYLVRPFAGTILGIIGDLIGRR
RLLLFTILLMGGCSLCMGLMPGYAQWGLFASFAFVVLRIMQGIALGGELPGAYVIVYESVKGKIGFATAILFTFVTVGFL
FSDFVGFTLEYIFGDFAWRAGFIIGGLLGFVGYYVRRNLNETREFENIDKQKRHSFSSLISTYGANLFAGICMVIIVAFG
GVMLTLYIHKFVEDILVSYNSGQISLILMPSVLTLTCFTFVFGFLSDKVGIAKMFTLGAGFIVVCALPVFYLMSSIGSVS
AIVITSVIIMLCYALVAATFIFLLCDLFPTDVRLSGVGLSYNLAFAIVGGVAPLLSTTIITMTAYNFLGPAIVGIVCGVL
GLLGMFIYLRKGGYHKTNKDMIVKL
>Mature_425_residues
MYDRDSRNVILLAGIGAILEFYDFVLYMIFSKEISATFFAQITNPTIKAFLTVLIFSIAYLVRPFAGTILGIIGDLIGRR
RLLLFTILLMGGCSLCMGLMPGYAQWGLFASFAFVVLRIMQGIALGGELPGAYVIVYESVKGKIGFATAILFTFVTVGFL
FSDFVGFTLEYIFGDFAWRAGFIIGGLLGFVGYYVRRNLNETREFENIDKQKRHSFSSLISTYGANLFAGICMVIIVAFG
GVMLTLYIHKFVEDILVSYNSGQISLILMPSVLTLTCFTFVFGFLSDKVGIAKMFTLGAGFIVVCALPVFYLMSSIGSVS
AIVITSVIIMLCYALVAATFIFLLCDLFPTDVRLSGVGLSYNLAFAIVGGVAPLLSTTIITMTAYNFLGPAIVGIVCGVL
GLLGMFIYLRKGGYHKTNKDMIVKL

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=29.81220657277, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788292, Length=409, Percent_Identity=27.1393643031785, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788942, Length=397, Percent_Identity=26.7002518891688, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=27.5689223057644, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=25.3886010362694, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082231, Length=434, Percent_Identity=23.7327188940092, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46411; Mature: 46411

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEC
>Mature Secondary Structure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CEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]