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The map label for this gene is gloA [H]

Identifier: 89256121

GI number: 89256121

Start: 721716

End: 722099

Strand: Direct

Name: gloA [H]

Synonym: FTL_0732

Alternate gene names: 89256121

Gene position: 721716-722099 (Clockwise)

Preceding gene: 89256119

Following gene: 89256122

Centisome position: 38.07

GC content: 34.11

Gene sequence:

>384_bases
ATGCGTTTTGCTCATGTAATGTTACGGGTAAAAGACTTAGATAAGTCTATCGATTTTTATACAAATGTACTAGGGATGAC
AGTGCAGAAAAAAATTGATAATACAGAGTATAAGTATACTTTAGCATTTTTAGGTTATGGAGATATCTCTAGCCATACAG
TTCTAGAGTTAACATATAATTGGGGCGAGCATGAGTATGATCATAGTAATGCTTTTGGACATTTATGTATGCAAGTAGAG
GATGTCTACAAAGCTTGTGATGATGTCAAAGCAAAAGGTGGAGTTGTAACTCGTGAGGCAGGTCCTGTAAAAGGTGGCAC
TCAAATTATTGCATTTATAAAAGATCCAGATGGTTATCAAATAGAACTTATTGAAAAAGCTTAA

Upstream 100 bases:

>100_bases
TTTGTTAACCCTGGATAGTTGCGAACAGGGGATTAAAAATCTGCTATAATATAACAAAATAGTTTATCAATAACAAATAA
ATTTTTTATAAGGAGACTAG

Downstream 100 bases:

>100_bases
TGAATAAAGATAGATTAAATATATTAGTTATAAGAGGCTATTAAAGTAAGGGTGATAGTGTTGAGCATATCGATGTTGAT
GGTATAAAAGTTAAGGTAAC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MRFAHVMLRVKDLDKSIDFYTNVLGMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYNWGEHEYDHSNAFGHLCMQVE
DVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQIELIEKA

Sequences:

>Translated_127_residues
MRFAHVMLRVKDLDKSIDFYTNVLGMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYNWGEHEYDHSNAFGHLCMQVE
DVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQIELIEKA
>Mature_127_residues
MRFAHVMLRVKDLDKSIDFYTNVLGMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYNWGEHEYDHSNAFGHLCMQVE
DVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQIELIEKA

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=144, Percent_Identity=39.5833333333333, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787940, Length=126, Percent_Identity=57.1428571428571, Blast_Score=164, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=36.6906474820144, Blast_Score=94, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=38.3561643835616, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14370; Mature: 14370

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFAHVMLRVKDLDKSIDFYTNVLGMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYN
CCHHHEEEEEHHCCCHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGEHEYDHSNAFGHLCMQVEDVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCE
IELIEKA
EEEEECC
>Mature Secondary Structure
MRFAHVMLRVKDLDKSIDFYTNVLGMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYN
CCHHHEEEEEHHCCCHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGEHEYDHSNAFGHLCMQVEDVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCE
IELIEKA
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]