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The map label for this gene is ligA [H]

Identifier: 89256066

GI number: 89256066

Start: 662852

End: 664888

Strand: Direct

Name: ligA [H]

Synonym: FTL_0676

Alternate gene names: 89256066

Gene position: 662852-664888 (Clockwise)

Preceding gene: 89256060

Following gene: 89256067

Centisome position: 34.96

GC content: 35.2

Gene sequence:

>2037_bases
ATGACTCCAAACGAATTTTTTTCTATAAAATATCATATTTTAGCTAAAGCAGAATTAAAAGCTTACATAGACAAATTAGC
TGATTATCTTAGTCAACAGAGCTATTTGTATCATACCTTAGATAAGCCAATTATTTCTGACTCTGATTATGATAAGCTGT
TTAGATTACTTCAAGATTTAGTTAATGACAATCCCCAATTTAAGCCGATAAATTCAGTATTAGATCGTGTTGGAGGTGAG
GTTTTAGCAGGGTTTGAAACTATCAAGCATAAAAAGAAAATGACATCTTTGGCAAATGTTTTTAGTCTAGAGGAGTTGCG
TGATTTTTATGACAAGATAGAGTATGATATTGAGCTTGAATGTGAACCAAAAATGGACGGTCTAGCAATCAGCATTTTTT
ATAAAAATGGTAAGTTTGATTATGCTGTTACACGTGGTGATGGTATTCAGGGTGAAAAAGTTTCAGAAAATGTTAAAACT
ATCCGCAATGTCCCACTTAAACTAAATACTTCAAACCCTCCAGAAGAACTAGAAGTTCGTGGTGAGATTATCTTAGATAA
GCAAAGTTTTCTATCTCTTAATGAGTATATGCAAACTCATGAGAATAAAACTTTTGCAAATCCGCGAAATGCAGCAGCTG
GAAGTATTCGTATGCTTGACTCAAAAGTTGTAGCAAAGCGACCACTTAAGCTTTATAGCTATGGTATTGGCTATTTTTCT
AAAGACTTTGTATATCCTGAAACCCAGTTTGAACTAATGCAGCTACTCCAAAGTTTTGGCTTTACAATTAGTGATAATAT
GTTTTTGGCAAAAAATTTCTCAGAGGTTGAGGAGTATCACCATAAGATGAGTCATCAGCGTGCTGATTTAGCTTATGATA
TTGATGGTTTAGTTTTTAAGGTTAATAATATTAAACTACAAGATACAATTGGCTACACTGCTAGAGGACCTAAGTGGGCT
ATAGCATATAAGTTCCCAGCTGAAGAGGTGGAGTCAGAAGTGCTAAATGTAGAATTCCAAGTTGGCAGAACAGGAGCAAT
TACGCCAGTAGCAAGACTTAAGCCAGTCGCAGTTGGCGGCGTAATAGTTTCAAATGCAACCTTGCACAATATCAATGAAA
TTAAACGCAAAGATATCCGAGTTGGTGATAGAGTAATCGTACGTAGAGCAGGAGATGTAATTCCAGAAGTGGTCAAAAGC
TTACCACAATATCGTAAGTCTGATGCACAGATAGTTGAAATGCCAACAAATTGTCCAGTTTGTGATTCAAAGATTGAGAA
TGTTAATGATCAGGCTATATATCGTTGTACAGGAGGATGGCATTGTCAGGCACAAACTACTGAGCGCTTGAAGCACTTCG
TCTCACGTAAAGCAATGGATATTGATAAGCTTGGAGCTAAACTAATTGAACAACTTGTTGCTGCAAATTTAATTAAGTAT
CCTGCTGATATTTACAAACTCAATTTTGAGCAATTGACAGGCTTAGAAAGAATGGCTGCTAAATCATCACAAAATGTCTT
AGACTCTATCACAAAAAGTAAAGAGCCAAGTTTAGCAAGGTTTATTTTTGCAATAGGGATTAAAGATATCGGTGAAGTAT
CATCAGACGCTTTAGCGAATCATTTTGGTAGTCTAGAGAGCTTCCGTGATGCTAAGTTTGAGGAGCTAATAGAGATTAAT
TATATTGGTGAGATTATAGCTAATAATATTGTTTCATTTTGGCATGATTCTCTAAATATTAAGATTGTAGAAGAGTTTTT
AGCTATTGGGATTAAGATACAAAATCCAGTAAAAGTTGAACACGCCTATAACGAGAGCTTCACAGGTAAAACTGTTGTTA
TTACTGGGTCTTTTGAGAACTATGGTCGTACAGAACTTACACAGCTTCTTAAGTCAATCGGGGCAAAAGTTACCTCAAGT
GTTTCTAAAAAAACTGACATGGTTATTTGTGGCGATAATGCTGGTAGCAAACTTACAAAAGCCCAAGAATTAGGTGTAGA
AGTTATTCTTGAAGATAATCTGAAAGATTTATTATGA

Upstream 100 bases:

>100_bases
AAATAGGAAGCTATAACTCTAGAGACCTTTCTCTTATGCAAAAGCTACTAAATTAGTATAAAATAGTTACTACAAAATAA
TTATTTAAAAATATTCTTTA

Downstream 100 bases:

>100_bases
ATATCAATAACTTAAAAAAGGGCGATGTAGTTTTCATAGTAGATAAGGCTGAGAAGAATATTTCTAGTTTATCTACCGGT
TTTGGTGGTTATAGTTACTA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MTPNEFFSIKYHILAKAELKAYIDKLADYLSQQSYLYHTLDKPIISDSDYDKLFRLLQDLVNDNPQFKPINSVLDRVGGE
VLAGFETIKHKKKMTSLANVFSLEELRDFYDKIEYDIELECEPKMDGLAISIFYKNGKFDYAVTRGDGIQGEKVSENVKT
IRNVPLKLNTSNPPEELEVRGEIILDKQSFLSLNEYMQTHENKTFANPRNAAAGSIRMLDSKVVAKRPLKLYSYGIGYFS
KDFVYPETQFELMQLLQSFGFTISDNMFLAKNFSEVEEYHHKMSHQRADLAYDIDGLVFKVNNIKLQDTIGYTARGPKWA
IAYKFPAEEVESEVLNVEFQVGRTGAITPVARLKPVAVGGVIVSNATLHNINEIKRKDIRVGDRVIVRRAGDVIPEVVKS
LPQYRKSDAQIVEMPTNCPVCDSKIENVNDQAIYRCTGGWHCQAQTTERLKHFVSRKAMDIDKLGAKLIEQLVAANLIKY
PADIYKLNFEQLTGLERMAAKSSQNVLDSITKSKEPSLARFIFAIGIKDIGEVSSDALANHFGSLESFRDAKFEELIEIN
YIGEIIANNIVSFWHDSLNIKIVEEFLAIGIKIQNPVKVEHAYNESFTGKTVVITGSFENYGRTELTQLLKSIGAKVTSS
VSKKTDMVICGDNAGSKLTKAQELGVEVILEDNLKDLL

Sequences:

>Translated_678_residues
MTPNEFFSIKYHILAKAELKAYIDKLADYLSQQSYLYHTLDKPIISDSDYDKLFRLLQDLVNDNPQFKPINSVLDRVGGE
VLAGFETIKHKKKMTSLANVFSLEELRDFYDKIEYDIELECEPKMDGLAISIFYKNGKFDYAVTRGDGIQGEKVSENVKT
IRNVPLKLNTSNPPEELEVRGEIILDKQSFLSLNEYMQTHENKTFANPRNAAAGSIRMLDSKVVAKRPLKLYSYGIGYFS
KDFVYPETQFELMQLLQSFGFTISDNMFLAKNFSEVEEYHHKMSHQRADLAYDIDGLVFKVNNIKLQDTIGYTARGPKWA
IAYKFPAEEVESEVLNVEFQVGRTGAITPVARLKPVAVGGVIVSNATLHNINEIKRKDIRVGDRVIVRRAGDVIPEVVKS
LPQYRKSDAQIVEMPTNCPVCDSKIENVNDQAIYRCTGGWHCQAQTTERLKHFVSRKAMDIDKLGAKLIEQLVAANLIKY
PADIYKLNFEQLTGLERMAAKSSQNVLDSITKSKEPSLARFIFAIGIKDIGEVSSDALANHFGSLESFRDAKFEELIEIN
YIGEIIANNIVSFWHDSLNIKIVEEFLAIGIKIQNPVKVEHAYNESFTGKTVVITGSFENYGRTELTQLLKSIGAKVTSS
VSKKTDMVICGDNAGSKLTKAQELGVEVILEDNLKDLL
>Mature_677_residues
TPNEFFSIKYHILAKAELKAYIDKLADYLSQQSYLYHTLDKPIISDSDYDKLFRLLQDLVNDNPQFKPINSVLDRVGGEV
LAGFETIKHKKKMTSLANVFSLEELRDFYDKIEYDIELECEPKMDGLAISIFYKNGKFDYAVTRGDGIQGEKVSENVKTI
RNVPLKLNTSNPPEELEVRGEIILDKQSFLSLNEYMQTHENKTFANPRNAAAGSIRMLDSKVVAKRPLKLYSYGIGYFSK
DFVYPETQFELMQLLQSFGFTISDNMFLAKNFSEVEEYHHKMSHQRADLAYDIDGLVFKVNNIKLQDTIGYTARGPKWAI
AYKFPAEEVESEVLNVEFQVGRTGAITPVARLKPVAVGGVIVSNATLHNINEIKRKDIRVGDRVIVRRAGDVIPEVVKSL
PQYRKSDAQIVEMPTNCPVCDSKIENVNDQAIYRCTGGWHCQAQTTERLKHFVSRKAMDIDKLGAKLIEQLVAANLIKYP
ADIYKLNFEQLTGLERMAAKSSQNVLDSITKSKEPSLARFIFAIGIKDIGEVSSDALANHFGSLESFRDAKFEELIEINY
IGEIIANNIVSFWHDSLNIKIVEEFLAIGIKIQNPVKVEHAYNESFTGKTVVITGSFENYGRTELTQLLKSIGAKVTSSV
SKKTDMVICGDNAGSKLTKAQELGVEVILEDNLKDLL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=663, Percent_Identity=46.3046757164404, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI87082305, Length=413, Percent_Identity=24.2130750605327, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 76467; Mature: 76336

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50172 BRCT ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNEFFSIKYHILAKAELKAYIDKLADYLSQQSYLYHTLDKPIISDSDYDKLFRLLQDL
CCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
VNDNPQFKPINSVLDRVGGEVLAGFETIKHKKKMTSLANVFSLEELRDFYDKIEYDIELE
HCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CEPKMDGLAISIFYKNGKFDYAVTRGDGIQGEKVSENVKTIRNVPLKLNTSNPPEELEVR
ECCCCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCHHHEEC
GEIILDKQSFLSLNEYMQTHENKTFANPRNAAAGSIRMLDSKVVAKRPLKLYSYGIGYFS
CEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHCCCCCC
KDFVYPETQFELMQLLQSFGFTISDNMFLAKNFSEVEEYHHKMSHQRADLAYDIDGLVFK
CCCCCCCHHHHHHHHHHHCCCEEECCEEEECCHHHHHHHHHHHHHCCCCCEEECCCEEEE
VNNIKLQDTIGYTARGPKWAIAYKFPAEEVESEVLNVEFQVGRTGAITPVARLKPVAVGG
EECEEEEECCCCCCCCCEEEEEEECCHHHHHHHHEEEEEEECCCCCCCHHHHCCCEEECC
VIVSNATLHNINEIKRKDIRVGDRVIVRRAGDVIPEVVKSLPQYRKSDAQIVEMPTNCPV
EEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
CDSKIENVNDQAIYRCTGGWHCQAQTTERLKHFVSRKAMDIDKLGAKLIEQLVAANLIKY
CCHHHCCCCCCEEEEECCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PADIYKLNFEQLTGLERMAAKSSQNVLDSITKSKEPSLARFIFAIGIKDIGEVSSDALAN
CHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
HFGSLESFRDAKFEELIEINYIGEIIANNIVSFWHDSLNIKIVEEFLAIGIKIQNPVKVE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCEEEE
HAYNESFTGKTVVITGSFENYGRTELTQLLKSIGAKVTSSVSKKTDMVICGDNAGSKLTK
ECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
AQELGVEVILEDNLKDLL
HHHCCEEEEEECCHHHCC
>Mature Secondary Structure 
TPNEFFSIKYHILAKAELKAYIDKLADYLSQQSYLYHTLDKPIISDSDYDKLFRLLQDL
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
VNDNPQFKPINSVLDRVGGEVLAGFETIKHKKKMTSLANVFSLEELRDFYDKIEYDIELE
HCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CEPKMDGLAISIFYKNGKFDYAVTRGDGIQGEKVSENVKTIRNVPLKLNTSNPPEELEVR
ECCCCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCHHHEEC
GEIILDKQSFLSLNEYMQTHENKTFANPRNAAAGSIRMLDSKVVAKRPLKLYSYGIGYFS
CEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHCCCCCC
KDFVYPETQFELMQLLQSFGFTISDNMFLAKNFSEVEEYHHKMSHQRADLAYDIDGLVFK
CCCCCCCHHHHHHHHHHHCCCEEECCEEEECCHHHHHHHHHHHHHCCCCCEEECCCEEEE
VNNIKLQDTIGYTARGPKWAIAYKFPAEEVESEVLNVEFQVGRTGAITPVARLKPVAVGG
EECEEEEECCCCCCCCCEEEEEEECCHHHHHHHHEEEEEEECCCCCCCHHHHCCCEEECC
VIVSNATLHNINEIKRKDIRVGDRVIVRRAGDVIPEVVKSLPQYRKSDAQIVEMPTNCPV
EEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
CDSKIENVNDQAIYRCTGGWHCQAQTTERLKHFVSRKAMDIDKLGAKLIEQLVAANLIKY
CCHHHCCCCCCEEEEECCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PADIYKLNFEQLTGLERMAAKSSQNVLDSITKSKEPSLARFIFAIGIKDIGEVSSDALAN
CHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
HFGSLESFRDAKFEELIEINYIGEIIANNIVSFWHDSLNIKIVEEFLAIGIKIQNPVKVE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCEEEE
HAYNESFTGKTVVITGSFENYGRTELTQLLKSIGAKVTSSVSKKTDMVICGDNAGSKLTK
ECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
AQELGVEVILEDNLKDLL
HHHCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA