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The map label for this gene is nusB [H]

Identifier: 89256034

GI number: 89256034

Start: 626753

End: 627178

Strand: Direct

Name: nusB [H]

Synonym: FTL_0643

Alternate gene names: 89256034

Gene position: 626753-627178 (Clockwise)

Preceding gene: 89256029

Following gene: 89256036

Centisome position: 33.06

GC content: 33.1

Gene sequence:

>426_bases
ATGAAAACTACCGCTAGAGCTCGAAATAATGCTCGTTTATATGCCGTACAAGCTTTGTACCAAAAGAAAATCGCTGATAA
TACATTCTCTGAGCTTAAGATTCAGTACTATGCTGATAATGCTGATAGGCATTATACTGATTGGGATCTTTTCTATAGAC
TTATAGATGCAGTTAAGACAAATCAAGACACTATAGACAAATATATCAAAGAAAACTCAAGTAATGGTGTTGAATCAATC
AATTATGTTGACTATGTAGTATTGCAAGTAGCTATAGCAGAACTGATAGAGTGTCTTGAGAATCCATATCAAGTGATTAT
AAAAGAGTATGTTGAGATATGTTACAGCATGGGTACTGAAGAAGGTTATAAGTTTATTAATGCAGTATTGCAAAATCTAG
CTAAGTCGATTAGAAGTGAAGAGTAA

Upstream 100 bases:

>100_bases
TTTAGTAGTATTAAAGTTCATAAGAACTATATATATTATTGTTAATAAGGTATAATCACAAGCTATAAAATTCGCTAATC
AATTACATCTACATAAAATT

Downstream 100 bases:

>100_bases
ATTTATCTTGAGATGCAAGAAAATTAAACTTGCTACGATGTTGTAATTGTTTATCTTTATCCAAAGTTGCACTTAAAGTA
GCTTGTTTGAATTCTTGATG

Product: N utilisation substance protein B

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MKTTARARNNARLYAVQALYQKKIADNTFSELKIQYYADNADRHYTDWDLFYRLIDAVKTNQDTIDKYIKENSSNGVESI
NYVDYVVLQVAIAELIECLENPYQVIIKEYVEICYSMGTEEGYKFINAVLQNLAKSIRSEE

Sequences:

>Translated_141_residues
MKTTARARNNARLYAVQALYQKKIADNTFSELKIQYYADNADRHYTDWDLFYRLIDAVKTNQDTIDKYIKENSSNGVESI
NYVDYVVLQVAIAELIECLENPYQVIIKEYVEICYSMGTEEGYKFINAVLQNLAKSIRSEE
>Mature_141_residues
MKTTARARNNARLYAVQALYQKKIADNTFSELKIQYYADNADRHYTDWDLFYRLIDAVKTNQDTIDKYIKENSSNGVESI
NYVDYVVLQVAIAELIECLENPYQVIIKEYVEICYSMGTEEGYKFINAVLQNLAKSIRSEE

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=125, Percent_Identity=34.4, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 16433; Mature: 16433

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTARARNNARLYAVQALYQKKIADNTFSELKIQYYADNADRHYTDWDLFYRLIDAVKT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHEEHEEECCCCCCCCHHHHHHHHHHHHHC
NQDTIDKYIKENSSNGVESINYVDYVVLQVAIAELIECLENPYQVIIKEYVEICYSMGTE
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EGYKFINAVLQNLAKSIRSEE
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTTARARNNARLYAVQALYQKKIADNTFSELKIQYYADNADRHYTDWDLFYRLIDAVKT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHEEHEEECCCCCCCCHHHHHHHHHHHHHC
NQDTIDKYIKENSSNGVESINYVDYVVLQVAIAELIECLENPYQVIIKEYVEICYSMGTE
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EGYKFINAVLQNLAKSIRSEE
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA