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The map label for this gene is rlmE [H]

Identifier: 89255843

GI number: 89255843

Start: 398792

End: 399412

Strand: Reverse

Name: rlmE [H]

Synonym: FTL_0433

Alternate gene names: 89255843

Gene position: 399412-398792 (Counterclockwise)

Preceding gene: 89255845

Following gene: 89255836

Centisome position: 21.07

GC content: 33.01

Gene sequence:

>621_bases
ATGTCAAAAGGTTCTAGTACTAAAAAATGGTTACATGAGCATACTTCGGATTACTATGTTATCCAAGCAAATAAGCTTGG
CTATCGTAGTAGAGCAAGCTTTAAAATTCTTGAAATACAAGATAAATATCAATTATTTAAACCAAATATGTTTGTTGTCG
ATCTTGGTGCATCTCCAGGTGGTTGGTCTGAGCAAGTTATAAAATATATAGGTAAAAATGGTAAGCTTATTGCTCTAGAT
CTACTAGAAATGGCTCCAATAGCTGGAGTAGAGTTTATTCAAGGCGACTTCTCTAGTGATGAAACTTACCAAAAACTCAA
TACTCTTGTTAATAATCAAAAGATTGATTGTGTTATCTCTGATATGGCGCCAAATCTAAGTGGTAACAAAACTTCAGATC
AAGCAAAATCGATATATTTGCTTGAATTAGCTTTGGATTTTGCTAATACTAATCTCAACAAAAACGGCAGTTTCGTTGCA
AAAGTCTTTCAAGGTCAAGGCAGTGATGAATATCTCAAATTAGTAAGAGAGTCTTTTAATAAAGTGATTCAATTTAAACC
AAAATCTTCAAGAGCAAAGTCACGAGAGTTTTATGTTATCGCAACTGAATTTAAGGGTTGA

Upstream 100 bases:

>100_bases
TATTTTATAAGCTTTTATAATATACAATTCTAATAACTCCAAATCTTTATTTATTATAAAAATATCCATACAATATACAA
AAAAATAATTAGCTATATAA

Downstream 100 bases:

>100_bases
CTTATCCTTCAAACACATAATTTATAAATAGAATTTTTAAGATTAGTCTATTTTATAGTTTATTTAAAAAAGTCTCCGCT
AAAATTTTCTTCTAACTTTT

Product: ribosomal large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGASPGGWSEQVIKYIGKNGKLIALD
LLEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVISDMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVA
KVFQGQGSDEYLKLVRESFNKVIQFKPKSSRAKSREFYVIATEFKG

Sequences:

>Translated_206_residues
MSKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGASPGGWSEQVIKYIGKNGKLIALD
LLEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVISDMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVA
KVFQGQGSDEYLKLVRESFNKVIQFKPKSSRAKSREFYVIATEFKG
>Mature_205_residues
SKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGASPGGWSEQVIKYIGKNGKLIALDL
LEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVISDMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVAK
VFQGQGSDEYLKLVRESFNKVIQFKPKSSRAKSREFYVIATEFKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=206, Percent_Identity=31.0679611650485, Blast_Score=131, Evalue=4e-31,
Organism=Homo sapiens, GI29029591, Length=187, Percent_Identity=34.7593582887701, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI29029589, Length=187, Percent_Identity=34.7593582887701, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI7110661, Length=187, Percent_Identity=34.7593582887701, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=29.7872340425532, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1789569, Length=200, Percent_Identity=53.5, Blast_Score=222, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17553474, Length=202, Percent_Identity=29.7029702970297, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=33.5078534031414, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71987550, Length=185, Percent_Identity=31.3513513513514, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17553860, Length=188, Percent_Identity=32.9787234042553, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=188, Percent_Identity=32.9787234042553, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=195, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=34.0314136125654, Blast_Score=108, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321302, Length=280, Percent_Identity=27.1428571428571, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21356387, Length=213, Percent_Identity=31.924882629108, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=32.8282828282828, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=32.6530612244898, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18859957, Length=179, Percent_Identity=31.8435754189944, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23176; Mature: 23044

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGASPG
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCHHEECCCEEEEECCCCCC
GWSEQVIKYIGKNGKLIALDLLEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVIS
CHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEEE
DMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVAKVFQGQGSDEYLKLVRESFN
CCCCCCCCCCCCCHHHEEEEEEEEEHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KVIQFKPKSSRAKSREFYVIATEFKG
HHEEECCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGASPG
CCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCHHEECCCEEEEECCCCCC
GWSEQVIKYIGKNGKLIALDLLEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVIS
CHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEEE
DMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVAKVFQGQGSDEYLKLVRESFN
CCCCCCCCCCCCCHHHEEEEEEEEEHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KVIQFKPKSSRAKSREFYVIATEFKG
HHEEECCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA