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The map label for this gene is kefA [C]

Identifier: 89055018

GI number: 89055018

Start: 2525584

End: 2527284

Strand: Direct

Name: kefA [C]

Synonym: Jann_2527

Alternate gene names: 89055018

Gene position: 2525584-2527284 (Clockwise)

Preceding gene: 89055017

Following gene: 89055019

Centisome position: 58.49

GC content: 62.14

Gene sequence:

>1701_bases
ATGACTGCATTAAAGTTTTTCCGCGCCAGCATTGGCGCCATCTTCGTTTCTGTCTTTGTTTGCTTCGCTCTGCTTGCCGT
GACGTCCGCGCCGACCTGGGCGCAGGACGATCCGTTCTACGACATTGATGGCTTGAACCCCGGCCTTGGTGCCGTACCGG
ACTCCATCGACAGATCCACGCCGCGGTCCGCCATGGAGAGTTTTCTGCGCGCAGCTGACGAGGAGGATTGGGCGGCGGCC
GCCCATCTACTGGATCTCGGCGCGTTATCTTCAGCAGATCAGGCCCGCGACGGCGCAACGCTTGCGCAGAAGTTGCATAT
GATTGTCGAACGGCGTGCGCTGCTGGATTGGTCGCTTCTGGTGGATCGGCCCGATGGCCTTGATGCGCGCGCATCCGATC
AGGACACGATGGGAGGACAGCCGCGCCGGTCCCTTCTGCTGCGGGAACTGGCGCTTGATCCTGTTCCCGCGACCCTGCGC
CTGAACCGCGTTCGTCCCGGCGGCGACGGAACGGAGCTTGTCTGGGTCTTCCCCCGCGCCACGGTGGATGAGATTGAGCC
TCTCTTCGACCGCTACGGTCCCTCCCCATTTGAGCAGATGTTGCCCTCCGCGCTGCGGGCAGAGGCCGTGGGTGGGCTGA
TGATCTGGGAGTTGATCGGCTTGCCCCTGCTTATCACTCTGGCCTGTCTTGCCGGTTGGCTTGCCGCATTCGGCTTTCGG
CTGGGGCGGCAATGGCTCCACCACGCGTCGCATAAATTGCTCGACGCGGCGCGCCTGCCCGCAATTATCGCAGCGGTGAC
GGCTGTGCTCTCCTGGGGCACGCAGGAGGTTTTTGTGTTCTCCTCCGTGTTGTCTGTCTTCATTTCCCCGCTTGTAGCGG
TCGGGTACACGACAGCGGCGCTGATGCTGGTGATGAACGGTCTCGGGGCCATTCTGGAGCGTCTTCTGGGTCCCGAAGCC
GCCGATCTGACCCACCGCGAAGTGGCGGAAGCGCGCGAAGCGGCCACACGCGTGGCGGCCCTGCGCCATATCCTGACGGT
GCTGGTCTTTGTCATCGGGGCAGGCATCGTGCTGTCGAGTGCCAGTATCTTCCGCAGCTTTGGCCTGTCGCTTCTTGCCT
CCGCCGGTGCCTTGACGCTGATCATTGGCTTCGCCGCGCGTCAGGTTCTTGGAAACATCATGGCAAGTCTGCAGATCGCC
CTGAACCAATCGGCCCGGGTCGGCGACCGCATCATGTGGCAGGACCGGCTGTGTTATGTCGAGAAGATCAACTTCACCTA
CATTCTGTTGCGCAATTGGGATGACACGCGCGTCGTCGTGCCAGTGTCCGAATTCGTGACCGAGACGTTCGACAACTGGA
CCCTGGAAGATCCGGGCATCAAGCGGATCCTCAAGCTGAAGCTGTCGCCAGAGGCAGATGTCGCCGCCTTGCGGGAGGCC
TTCAACGACATCATGGATGACATGAAAGCGGGCGAGTTGGGCAGCGAGTTGGGTGATCTAGAAGAGGCGACCGTGAATGT
CGCGGGTCAGGATGCGTTGGGCGTCGATGTCTGGTTTGCTGTGCCATGCCTTGATCCCTCAACCCAATGGGAGGTGTCCT
GCGAAGCCCGCGAGCGGTTGATCAAGGCCGCGTCCCAGATTGCCGAGCAGACGGGCCGGGCGGTGTTCCCTGAAGGCGCG
GCATTGGATGCCGCGGCATGA

Upstream 100 bases:

>100_bases
GATGATCCGGAAGAGGCCGTGGCCTGGGCCCGCCGCGCGTTGGAGCATCTTTAGCCGCTTCAATGATGGAACCAAACCCG
CGCGGGGGCGTTCTGGGCCT

Downstream 100 bases:

>100_bases
TGCGCCCCAACCCTGTGTGGTCAGGCAGCCAGTATGTGCAGCGCGCACGAACTCCATTGACCGGTGTCGGTTTGTAGTGG
CCATTTGACTAATCCTGCGG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Mechanosensitive Ion Channel Family Protein; Small-Conductance Mechanosensitive Channel; Mechanosensitive Ion Channel Protein MscS; Mechanosensitive Ion Channel MscS; Mscs Mechanosensitive Ion Channel; Mechanosensitive Ion Channel; Mechanosensitive Ion Channel Protein; Transporter MscS Family; Small-Conductance Mechanosensitive Channel-Like Protein; Small-Conductance Mechanosensitive Ion Channel

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MTALKFFRASIGAIFVSVFVCFALLAVTSAPTWAQDDPFYDIDGLNPGLGAVPDSIDRSTPRSAMESFLRAADEEDWAAA
AHLLDLGALSSADQARDGATLAQKLHMIVERRALLDWSLLVDRPDGLDARASDQDTMGGQPRRSLLLRELALDPVPATLR
LNRVRPGGDGTELVWVFPRATVDEIEPLFDRYGPSPFEQMLPSALRAEAVGGLMIWELIGLPLLITLACLAGWLAAFGFR
LGRQWLHHASHKLLDAARLPAIIAAVTAVLSWGTQEVFVFSSVLSVFISPLVAVGYTTAALMLVMNGLGAILERLLGPEA
ADLTHREVAEAREAATRVAALRHILTVLVFVIGAGIVLSSASIFRSFGLSLLASAGALTLIIGFAARQVLGNIMASLQIA
LNQSARVGDRIMWQDRLCYVEKINFTYILLRNWDDTRVVVPVSEFVTETFDNWTLEDPGIKRILKLKLSPEADVAALREA
FNDIMDDMKAGELGSELGDLEEATVNVAGQDALGVDVWFAVPCLDPSTQWEVSCEARERLIKAASQIAEQTGRAVFPEGA
ALDAAA

Sequences:

>Translated_566_residues
MTALKFFRASIGAIFVSVFVCFALLAVTSAPTWAQDDPFYDIDGLNPGLGAVPDSIDRSTPRSAMESFLRAADEEDWAAA
AHLLDLGALSSADQARDGATLAQKLHMIVERRALLDWSLLVDRPDGLDARASDQDTMGGQPRRSLLLRELALDPVPATLR
LNRVRPGGDGTELVWVFPRATVDEIEPLFDRYGPSPFEQMLPSALRAEAVGGLMIWELIGLPLLITLACLAGWLAAFGFR
LGRQWLHHASHKLLDAARLPAIIAAVTAVLSWGTQEVFVFSSVLSVFISPLVAVGYTTAALMLVMNGLGAILERLLGPEA
ADLTHREVAEAREAATRVAALRHILTVLVFVIGAGIVLSSASIFRSFGLSLLASAGALTLIIGFAARQVLGNIMASLQIA
LNQSARVGDRIMWQDRLCYVEKINFTYILLRNWDDTRVVVPVSEFVTETFDNWTLEDPGIKRILKLKLSPEADVAALREA
FNDIMDDMKAGELGSELGDLEEATVNVAGQDALGVDVWFAVPCLDPSTQWEVSCEARERLIKAASQIAEQTGRAVFPEGA
ALDAAA
>Mature_565_residues
TALKFFRASIGAIFVSVFVCFALLAVTSAPTWAQDDPFYDIDGLNPGLGAVPDSIDRSTPRSAMESFLRAADEEDWAAAA
HLLDLGALSSADQARDGATLAQKLHMIVERRALLDWSLLVDRPDGLDARASDQDTMGGQPRRSLLLRELALDPVPATLRL
NRVRPGGDGTELVWVFPRATVDEIEPLFDRYGPSPFEQMLPSALRAEAVGGLMIWELIGLPLLITLACLAGWLAAFGFRL
GRQWLHHASHKLLDAARLPAIIAAVTAVLSWGTQEVFVFSSVLSVFISPLVAVGYTTAALMLVMNGLGAILERLLGPEAA
DLTHREVAEAREAATRVAALRHILTVLVFVIGAGIVLSSASIFRSFGLSLLASAGALTLIIGFAARQVLGNIMASLQIAL
NQSARVGDRIMWQDRLCYVEKINFTYILLRNWDDTRVVVPVSEFVTETFDNWTLEDPGIKRILKLKLSPEADVAALREAF
NDIMDDMKAGELGSELGDLEEATVNVAGQDALGVDVWFAVPCLDPSTQWEVSCEARERLIKAASQIAEQTGRAVFPEGAA
LDAAA

Specific function: Unknown

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Integral Membrane Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786670, Length=148, Percent_Identity=27.7027027027027, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 61239; Mature: 61108

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALKFFRASIGAIFVSVFVCFALLAVTSAPTWAQDDPFYDIDGLNPGLGAVPDSIDRST
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
PRSAMESFLRAADEEDWAAAAHLLDLGALSSADQARDGATLAQKLHMIVERRALLDWSLL
CHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHE
VDRPDGLDARASDQDTMGGQPRRSLLLRELALDPVPATLRLNRVRPGGDGTELVWVFPRA
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEECCC
TVDEIEPLFDRYGPSPFEQMLPSALRAEAVGGLMIWELIGLPLLITLACLAGWLAAFGFR
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRQWLHHASHKLLDAARLPAIIAAVTAVLSWGTQEVFVFSSVLSVFISPLVAVGYTTAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMLVMNGLGAILERLLGPEAADLTHREVAEAREAATRVAALRHILTVLVFVIGAGIVLSS
HHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIFRSFGLSLLASAGALTLIIGFAARQVLGNIMASLQIALNQSARVGDRIMWQDRLCYV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHE
EKINFTYILLRNWDDTRVVVPVSEFVTETFDNWTLEDPGIKRILKLKLSPEADVAALREA
EECCEEEEEEECCCCCEEEEEHHHHHHHHHCCCCCCCCHHHHHHHEECCCCHHHHHHHHH
FNDIMDDMKAGELGSELGDLEEATVNVAGQDALGVDVWFAVPCLDPSTQWEVSCEARERL
HHHHHHHHHHHHHHHHHHCHHHHHHEECCCCCCCEEEEEEEECCCCCCCCEEEHHHHHHH
IKAASQIAEQTGRAVFPEGAALDAAA
HHHHHHHHHHCCCEECCCCCCCCCCC
>Mature Secondary Structure 
TALKFFRASIGAIFVSVFVCFALLAVTSAPTWAQDDPFYDIDGLNPGLGAVPDSIDRST
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
PRSAMESFLRAADEEDWAAAAHLLDLGALSSADQARDGATLAQKLHMIVERRALLDWSLL
CHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHE
VDRPDGLDARASDQDTMGGQPRRSLLLRELALDPVPATLRLNRVRPGGDGTELVWVFPRA
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEECCC
TVDEIEPLFDRYGPSPFEQMLPSALRAEAVGGLMIWELIGLPLLITLACLAGWLAAFGFR
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRQWLHHASHKLLDAARLPAIIAAVTAVLSWGTQEVFVFSSVLSVFISPLVAVGYTTAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMLVMNGLGAILERLLGPEAADLTHREVAEAREAATRVAALRHILTVLVFVIGAGIVLSS
HHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIFRSFGLSLLASAGALTLIIGFAARQVLGNIMASLQIALNQSARVGDRIMWQDRLCYV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHE
EKINFTYILLRNWDDTRVVVPVSEFVTETFDNWTLEDPGIKRILKLKLSPEADVAALREA
EECCEEEEEEECCCCCEEEEEHHHHHHHHHCCCCCCCCHHHHHHHEECCCCHHHHHHHHH
FNDIMDDMKAGELGSELGDLEEATVNVAGQDALGVDVWFAVPCLDPSTQWEVSCEARERL
HHHHHHHHHHHHHHHHHHCHHHHHHEECCCCCCCEEEEEEEECCCCCCCCEEEHHHHHHH
IKAASQIAEQTGRAVFPEGAALDAAA
HHHHHHHHHHCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA