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The map label for this gene is livK [C]

Identifier: 89054656

GI number: 89054656

Start: 2171220

End: 2172419

Strand: Reverse

Name: livK [C]

Synonym: Jann_2165

Alternate gene names: 89054656

Gene position: 2172419-2171220 (Counterclockwise)

Preceding gene: 89054662

Following gene: 89054655

Centisome position: 50.31

GC content: 65.42

Gene sequence:

>1200_bases
ATGATGAAAAAGTTTTTGCTGGCATCCGCCGCTTCCATTGCGCTGACAGGCGCTGCCGCCGCCGATGGCCACCAGGGCGA
TGTGACGCTTGGCGTGATCCTCGGCTTCACCGGTCCGATTGAATCGATCACACCCGCCATGGGCGACGGCGCAGAGCTGG
CGATGGCCGAAGTCACCGACAGCGGTATGTTGTTTGGCGGCGCGGCCGTTACGTCTGTTCGGGCTGACTCGACCTGCGTT
GACAGTTCCGCCGCACAAGCGGCCGCCGAGCGTCTGATCACGTCCGATGGCGTGCACGGCATTGTCGGCGCCGATTGCTC
CGGCGTCACCGGCTCCATTCTGGCCAACGTCGCCGTGCCCAACGGCATGGTGATGATCTCGCCGTCCGCCACCTCCCCCG
GCCTGTCGACGGCAGAGGATAACGGGTTGTTCTTCCGCACCGCGCCGTCCGATGCGCGCCAGGGCGTCGTGATGGCCGAA
ATCATCATGGAACGCGGCATCGACACCGTCGCGCTGACCTACACCAACAACGACTATGGCCAGGGCCTGGCCGATGCCTT
CTCTGCGGCGTTTGAAGAAGCGGGCGGCACCGTCACGCTGTCGGCGGCCCATGAGGATGGCCGCGCGGATTACTCCGCCG
AAGTCGGCGCACTTGCCTCCGCCGGTGGTGACGCGCTTGTCGTTGCGGGCTACGTGGACCAGGGCGGCTCCGGCGTGATC
CAGGCTGCATTAGATACCGGCGCGTTTGACACCTTCGTGTTCCCCGACGGCATGGTCTCTCAGGCGCTGGTCGACAATTT
CGGCTCCGACATCGACGGGTCGTTCGGCCAGAACCCCGGCACCGACAGCGAAGGCGCGGGCCTCTATCAGGCCATGGCCG
AGGAAGCGGGCTTTGACGGCACCTCGCCGTTCTCCGCGGAATCCTATGACGCCGCCGCCCTGCTCATGCTCGCCATGGCC
GCTGCGGAAAGCCGCAACGCCGCCGACTATATGGGTGAGATCATGAACGTCGCCAACGCGCCCGGCACCGAGATCCTGCC
CGGCCAGCTTGGCATGGCGCTGGAAATGATCGCCAACGGGGAAGAGGTCGATTATGTCGGCGCCTCCGCCGTGGAGCTGA
TCGGGGGTGGCGAATCTGCCGGCAATTATCGTGAGATCGAGATCTCCGGCGGGGAGCTGACGGTCGCCGGTTACCGCTGA

Upstream 100 bases:

>100_bases
AAACGAAACGCCCTTTCCCTTTGCAGAACACACGGCTAATCTTGCGCGCATCAAGGACGATCAAATGTGAAATTTGGCAA
TGTCCACACCAGGGAGATAC

Downstream 100 bases:

>100_bases
TTGACCTCCCATTGAGAGAGGCGCAGCCCGGTTTCATCGCCGGGCTGCGTCTTTTTACACAGGCGCGCGCGCCGGGATTG
TCGGCCCAGAGGGACGCCCA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MMKKFLLASAASIALTGAAAADGHQGDVTLGVILGFTGPIESITPAMGDGAELAMAEVTDSGMLFGGAAVTSVRADSTCV
DSSAAQAAAERLITSDGVHGIVGADCSGVTGSILANVAVPNGMVMISPSATSPGLSTAEDNGLFFRTAPSDARQGVVMAE
IIMERGIDTVALTYTNNDYGQGLADAFSAAFEEAGGTVTLSAAHEDGRADYSAEVGALASAGGDALVVAGYVDQGGSGVI
QAALDTGAFDTFVFPDGMVSQALVDNFGSDIDGSFGQNPGTDSEGAGLYQAMAEEAGFDGTSPFSAESYDAAALLMLAMA
AAESRNAADYMGEIMNVANAPGTEILPGQLGMALEMIANGEEVDYVGASAVELIGGGESAGNYREIEISGGELTVAGYR

Sequences:

>Translated_399_residues
MMKKFLLASAASIALTGAAAADGHQGDVTLGVILGFTGPIESITPAMGDGAELAMAEVTDSGMLFGGAAVTSVRADSTCV
DSSAAQAAAERLITSDGVHGIVGADCSGVTGSILANVAVPNGMVMISPSATSPGLSTAEDNGLFFRTAPSDARQGVVMAE
IIMERGIDTVALTYTNNDYGQGLADAFSAAFEEAGGTVTLSAAHEDGRADYSAEVGALASAGGDALVVAGYVDQGGSGVI
QAALDTGAFDTFVFPDGMVSQALVDNFGSDIDGSFGQNPGTDSEGAGLYQAMAEEAGFDGTSPFSAESYDAAALLMLAMA
AAESRNAADYMGEIMNVANAPGTEILPGQLGMALEMIANGEEVDYVGASAVELIGGGESAGNYREIEISGGELTVAGYR
>Mature_399_residues
MMKKFLLASAASIALTGAAAADGHQGDVTLGVILGFTGPIESITPAMGDGAELAMAEVTDSGMLFGGAAVTSVRADSTCV
DSSAAQAAAERLITSDGVHGIVGADCSGVTGSILANVAVPNGMVMISPSATSPGLSTAEDNGLFFRTAPSDARQGVVMAE
IIMERGIDTVALTYTNNDYGQGLADAFSAAFEEAGGTVTLSAAHEDGRADYSAEVGALASAGGDALVVAGYVDQGGSGVI
QAALDTGAFDTFVFPDGMVSQALVDNFGSDIDGSFGQNPGTDSEGAGLYQAMAEEAGFDGTSPFSAESYDAAALLMLAMA
AAESRNAADYMGEIMNVANAPGTEILPGQLGMALEMIANGEEVDYVGASAVELIGGGESAGNYREIEISGGELTVAGYR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=277, Percent_Identity=27.4368231046931, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789867, Length=320, Percent_Identity=26.25, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39810; Mature: 39810

Theoretical pI: Translated: 3.56; Mature: 3.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKFLLASAASIALTGAAAADGHQGDVTLGVILGFTGPIESITPAMGDGAELAMAEVTD
CCHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCCCCCCEEEEEEECC
SGMLFGGAAVTSVRADSTCVDSSAAQAAAERLITSDGVHGIVGADCSGVTGSILANVAVP
CCEEECCHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHEEEECC
NGMVMISPSATSPGLSTAEDNGLFFRTAPSDARQGVVMAEIIMERGIDTVALTYTNNDYG
CCEEEEECCCCCCCCCCCCCCCEEEEECCCHHHCCHHHHHHHHHCCCCEEEEEEECCCCC
QGLADAFSAAFEEAGGTVTLSAAHEDGRADYSAEVGALASAGGDALVVAGYVDQGGSGVI
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCEE
QAALDTGAFDTFVFPDGMVSQALVDNFGSDIDGSFGQNPGTDSEGAGLYQAMAEEAGFDG
EEEECCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
TSPFSAESYDAAALLMLAMAAAESRNAADYMGEIMNVANAPGTEILPGQLGMALEMIANG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
EEVDYVGASAVELIGGGESAGNYREIEISGGELTVAGYR
CCEEECCCCCEEEECCCCCCCCEEEEEEECCEEEEECCC
>Mature Secondary Structure
MMKKFLLASAASIALTGAAAADGHQGDVTLGVILGFTGPIESITPAMGDGAELAMAEVTD
CCHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCCCCCCEEEEEEECC
SGMLFGGAAVTSVRADSTCVDSSAAQAAAERLITSDGVHGIVGADCSGVTGSILANVAVP
CCEEECCHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHEEEECC
NGMVMISPSATSPGLSTAEDNGLFFRTAPSDARQGVVMAEIIMERGIDTVALTYTNNDYG
CCEEEEECCCCCCCCCCCCCCCEEEEECCCHHHCCHHHHHHHHHCCCCEEEEEEECCCCC
QGLADAFSAAFEEAGGTVTLSAAHEDGRADYSAEVGALASAGGDALVVAGYVDQGGSGVI
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCEE
QAALDTGAFDTFVFPDGMVSQALVDNFGSDIDGSFGQNPGTDSEGAGLYQAMAEEAGFDG
EEEECCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
TSPFSAESYDAAALLMLAMAAAESRNAADYMGEIMNVANAPGTEILPGQLGMALEMIANG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
EEVDYVGASAVELIGGGESAGNYREIEISGGELTVAGYR
CCEEECCCCCEEEECCCCCCCCEEEEEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA