Click here to switch to the map view.

The map label for this gene is nudF [H]

Identifier: 89054483

GI number: 89054483

Start: 1991211

End: 1992338

Strand: Direct

Name: nudF [H]

Synonym: Jann_1992

Alternate gene names: 89054483

Gene position: 1991211-1992338 (Clockwise)

Preceding gene: 89054482

Following gene: 89054484

Centisome position: 46.11

GC content: 65.07

Gene sequence:

>1128_bases
ATGGCCCCAATCTTTCTGTTTGGCACCCTGTGTCACCAGCCCCTGTTGGATCTTGTGGCAGGTATGTCCGTCCGGATGGA
GCCTGCGCATTTGCCGGGCTACCGCGCGGCTTGGGTCAAGGACGCGTCCTGGCCCATGCTGGAGGTGTGTGCGGATCAAC
ATGCGCAGGGTGCTCTCATCGACCCGGACCCGGACGCTTTGGCGCGGCTCGACTTTTATGAGGCCTGTTTCGGCTACGCC
CGCGCGGGCGTGACTGTGATCCGCGATGGGGCGGAGGTTGCGGCGGAGGCGTGGTTCCCGTCGACGCAGGCCGGGGTTCC
GGGCGCGGATTGGTCGCTACCTGACTGGGCGCGCCGGTATGGCGCGGCGCAGCTGCACGCCGCCACGGAAGTCATGCGCC
AGATGGGGCAACAAGCGCCGGAGGAGGTCGGCCGCAGATTTCCCATCATCCGCGCGCGGGCGGAATCGGTCGTGCGGGCC
GGCCAGTGGCAGCGACCGGGCCATGTGGGGGCGGGGATGACGCGCGCAGATGTCGTGGATCACGGCACGCACCACATTCA
CGACGGATTTTTCAACACACTTGATGTGCGGGTCAGCCACAGGCGGTTTGATGGCGGTTTGCAGGGCCCCCTTGACCGAT
CCGCGTACCGGGTGGTCGATGCCGTCACCGTGCTGCCCTATGATCCGGTACGGGACCGGATCCTGTTGATTGAGCAGTTT
CGGGTGGGTCCCTATGCCAATGGCGATGCCGCGCCGTGGCTGCTTGAGGCTATCGCAGGCATTCTGGATGCCGGGGAAAC
GGCGGAGGCGACGGCCCGACGTGAAGCGCAGGAGGAGGCGAACCTGACCTTGGACGAGCTGCACTTCGTTGCGCGTTACT
ATCCGACACCGGGCGGTGTGGCGCAGGTCCTCTTCTCTTATCTGGCGACGGCGGATTTGCCTGATGAGGTCGCGGGCCAG
GGCGGCCACGTGAGCGAGGGGGAGGATATTCTCAGCCACCTTGTGACCTATGATCTCGCCTGCCAGATGCTGCACGACGG
CGATATGGCGACCGCGCCTTTGATCCTGTCGATGCAATATCTGATGCTGCATCGTGATCGGTTGCGCGCCGCTGCCGGGT
TGGGTTGA

Upstream 100 bases:

>100_bases
TTCGCTTCAGGCGATGACCGTGCCGATCTGGACCGTCCTGATCGTGGGCGGTTTTGTGGGCGGAATACTGACAGAGCTTG
CCGCGAAAGTCTGGCGGTAG

Downstream 100 bases:

>100_bases
GTTCACCCAAGGCGCGGCCTATCCATGCGGTGAGACCTGCGCCGGTTATCCACGCGAGACCATCCACGAAGGGCCAATGT
CATGACGATCCACTCCGACC

Product: nucleoside diphosphate pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAPIFLFGTLCHQPLLDLVAGMSVRMEPAHLPGYRAAWVKDASWPMLEVCADQHAQGALIDPDPDALARLDFYEACFGYA
RAGVTVIRDGAEVAAEAWFPSTQAGVPGADWSLPDWARRYGAAQLHAATEVMRQMGQQAPEEVGRRFPIIRARAESVVRA
GQWQRPGHVGAGMTRADVVDHGTHHIHDGFFNTLDVRVSHRRFDGGLQGPLDRSAYRVVDAVTVLPYDPVRDRILLIEQF
RVGPYANGDAAPWLLEAIAGILDAGETAEATARREAQEEANLTLDELHFVARYYPTPGGVAQVLFSYLATADLPDEVAGQ
GGHVSEGEDILSHLVTYDLACQMLHDGDMATAPLILSMQYLMLHRDRLRAAAGLG

Sequences:

>Translated_375_residues
MAPIFLFGTLCHQPLLDLVAGMSVRMEPAHLPGYRAAWVKDASWPMLEVCADQHAQGALIDPDPDALARLDFYEACFGYA
RAGVTVIRDGAEVAAEAWFPSTQAGVPGADWSLPDWARRYGAAQLHAATEVMRQMGQQAPEEVGRRFPIIRARAESVVRA
GQWQRPGHVGAGMTRADVVDHGTHHIHDGFFNTLDVRVSHRRFDGGLQGPLDRSAYRVVDAVTVLPYDPVRDRILLIEQF
RVGPYANGDAAPWLLEAIAGILDAGETAEATARREAQEEANLTLDELHFVARYYPTPGGVAQVLFSYLATADLPDEVAGQ
GGHVSEGEDILSHLVTYDLACQMLHDGDMATAPLILSMQYLMLHRDRLRAAAGLG
>Mature_374_residues
APIFLFGTLCHQPLLDLVAGMSVRMEPAHLPGYRAAWVKDASWPMLEVCADQHAQGALIDPDPDALARLDFYEACFGYAR
AGVTVIRDGAEVAAEAWFPSTQAGVPGADWSLPDWARRYGAAQLHAATEVMRQMGQQAPEEVGRRFPIIRARAESVVRAG
QWQRPGHVGAGMTRADVVDHGTHHIHDGFFNTLDVRVSHRRFDGGLQGPLDRSAYRVVDAVTVLPYDPVRDRILLIEQFR
VGPYANGDAAPWLLEAIAGILDAGETAEATARREAQEEANLTLDELHFVARYYPTPGGVAQVLFSYLATADLPDEVAGQG
GHVSEGEDILSHLVTYDLACQMLHDGDMATAPLILSMQYLMLHRDRLRAAAGLG

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=205, Percent_Identity=32.6829268292683, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788810, Length=176, Percent_Identity=29.5454545454545, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 40937; Mature: 40806

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPIFLFGTLCHQPLLDLVAGMSVRMEPAHLPGYRAAWVKDASWPMLEVCADQHAQGALI
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCC
DPDPDALARLDFYEACFGYARAGVTVIRDGAEVAAEAWFPSTQAGVPGADWSLPDWARRY
CCCHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GAAQLHAATEVMRQMGQQAPEEVGRRFPIIRARAESVVRAGQWQRPGHVGAGMTRADVVD
CCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
HGTHHIHDGFFNTLDVRVSHRRFDGGLQGPLDRSAYRVVDAVTVLPYDPVRDRILLIEQF
CCCHHHHCCHHHHHHEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHEEHHHHH
RVGPYANGDAAPWLLEAIAGILDAGETAEATARREAQEEANLTLDELHFVARYYPTPGGV
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHH
AQVLFSYLATADLPDEVAGQGGHVSEGEDILSHLVTYDLACQMLHDGDMATAPLILSMQY
HHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LMLHRDRLRAAAGLG
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
APIFLFGTLCHQPLLDLVAGMSVRMEPAHLPGYRAAWVKDASWPMLEVCADQHAQGALI
CCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCC
DPDPDALARLDFYEACFGYARAGVTVIRDGAEVAAEAWFPSTQAGVPGADWSLPDWARRY
CCCHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GAAQLHAATEVMRQMGQQAPEEVGRRFPIIRARAESVVRAGQWQRPGHVGAGMTRADVVD
CCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
HGTHHIHDGFFNTLDVRVSHRRFDGGLQGPLDRSAYRVVDAVTVLPYDPVRDRILLIEQF
CCCHHHHCCHHHHHHEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHEEHHHHH
RVGPYANGDAAPWLLEAIAGILDAGETAEATARREAQEEANLTLDELHFVARYYPTPGGV
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHH
AQVLFSYLATADLPDEVAGQGGHVSEGEDILSHLVTYDLACQMLHDGDMATAPLILSMQY
HHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LMLHRDRLRAAAGLG
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]