Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 89054449

GI number: 89054449

Start: 1951311

End: 1952672

Strand: Direct

Name: cysS

Synonym: Jann_1958

Alternate gene names: 89054449

Gene position: 1951311-1952672 (Clockwise)

Preceding gene: 89054446

Following gene: 89054450

Centisome position: 45.19

GC content: 61.23

Gene sequence:

>1362_bases
ATGGTCGAAATCCACCTGAAGAATTCCAAGTCCCGCAAGGTCGAGCTGTTGCAGCCGATCGAGCCCGGTCGCGTGTCGCT
CTATCTCTGCGGGCCGACGGTCTATGACCGCGCGCATCTGGGCAATGCCCGCTCGGCGGTCGTGTTCGATCAGCTGTTTC
GGCTGCTGCGCCATGTCTACGGGGCGGAAAACGTGACCTTCGTGCGCAACTTCACGGATGTCGATGACAAGATCAACGCA
CGCGCTTTGGCCGAGGGGCGGGAGATCGCCGATCTGACCGCCGAGACGTCGCAATGGTATCTCGATGACACCCGCGCGCT
CGGCGTGCTGGACCCCAACCACATGCCCCGCGCAACCGAATATATCGCCGAGATGGTGGCCTTTATCGAGGATCTGATCG
CCAAGGGCTTCGCCTATGCTCGCGACGGCCACGCGCTGTTTCGGGTGCGCAGTTTTGACGATTATGGCAAGCTGTCGGGC
CGGTCGGTCGATGACATGATCGCAGGCGCACGGGTGGAGGTCGCGCCTTACAAAGAGGACCCGATGGATTTCGTCCTCTG
GAAGCCGTCGTCGGACGATATCCCGGGTTGGGACAGCCCGTGGGGCAGGGGCCGCCCCGGCTGGCACATCGAATGCTCCG
CCATGGCCCACGCGCTGTTGGGCGAGCAGTTTGATATCCACGGCGGCGGCGCGGACCTGATGTTCCCGCATCACGAAAAC
GAGGTCGCCCAATCCTGCGCCGCAGGCCACGGCTTCGCGAATATCTGGATGCACAACGAGATGTTGCAGGTCGAGGGCAA
GAAGATGTCCAAGTCGTTGGGCAACTTCTTCACCGTGCGCGATTTGCTCGATCAGGGCGTGCCGGGCGAAGTGATCCGGT
TTGTGATGCTATCCACCCACTATGGCAAGCCGATGGACTGGACCGAGAAGAAGCGGGCGGAGGCGGAGGCGACGCTGCGC
AAGTGGTATGCCGCTTTGCGTCAGAGCGTCGCTGAACAAGAGGCGACACCGCACCTTGTAGACTCTTTGGCCGACGACCT
TAATACGGCCTTGGCCATCACAACCCTGCATGGAATGTATGAAACCGGTAATTTTGGACCGCTTGCGGGTGGTTTGCGTT
TGCTTGGCCTTTGGAGCGGAACCGTGCCGGATTGGTCGGAGATAGCCGAGGTTGACCTGACCTATTTCGCTGGCCGGTTT
TCTTCGGCGCGCGCCGAAGCGATGGTGACCAAAGATTTCACCGACGTCGACGCCCTCAAATCCGCCCTTACCGCCGCAGG
CGTAGAAGTCCGCATGTCCAAGGACGGCGTTGAGCTTGTTCCGGGCCCAACCTTCGACCCCGCCAAGCTGGAGGATCTGT
GA

Upstream 100 bases:

>100_bases
CGCTAAGTCTTGCGGGCCGGGCACGTGTGGTTTTCTTGAACTCCTCCATCTGTTAGCTCGGTCGCGCGCCCTCCTACACG
CCTGCTTGAGAGCCAGAAAA

Downstream 100 bases:

>100_bases
TGCGCGCCATATCACAGGCCAGCCCCCGGTCGCGGGTGGCGGTACAGCCCCCGCCGTTTTGCCGGAGACAAACCTACGGT
TTGACGGGGGCGGTCCGGGG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MVEIHLKNSKSRKVELLQPIEPGRVSLYLCGPTVYDRAHLGNARSAVVFDQLFRLLRHVYGAENVTFVRNFTDVDDKINA
RALAEGREIADLTAETSQWYLDDTRALGVLDPNHMPRATEYIAEMVAFIEDLIAKGFAYARDGHALFRVRSFDDYGKLSG
RSVDDMIAGARVEVAPYKEDPMDFVLWKPSSDDIPGWDSPWGRGRPGWHIECSAMAHALLGEQFDIHGGGADLMFPHHEN
EVAQSCAAGHGFANIWMHNEMLQVEGKKMSKSLGNFFTVRDLLDQGVPGEVIRFVMLSTHYGKPMDWTEKKRAEAEATLR
KWYAALRQSVAEQEATPHLVDSLADDLNTALAITTLHGMYETGNFGPLAGGLRLLGLWSGTVPDWSEIAEVDLTYFAGRF
SSARAEAMVTKDFTDVDALKSALTAAGVEVRMSKDGVELVPGPTFDPAKLEDL

Sequences:

>Translated_453_residues
MVEIHLKNSKSRKVELLQPIEPGRVSLYLCGPTVYDRAHLGNARSAVVFDQLFRLLRHVYGAENVTFVRNFTDVDDKINA
RALAEGREIADLTAETSQWYLDDTRALGVLDPNHMPRATEYIAEMVAFIEDLIAKGFAYARDGHALFRVRSFDDYGKLSG
RSVDDMIAGARVEVAPYKEDPMDFVLWKPSSDDIPGWDSPWGRGRPGWHIECSAMAHALLGEQFDIHGGGADLMFPHHEN
EVAQSCAAGHGFANIWMHNEMLQVEGKKMSKSLGNFFTVRDLLDQGVPGEVIRFVMLSTHYGKPMDWTEKKRAEAEATLR
KWYAALRQSVAEQEATPHLVDSLADDLNTALAITTLHGMYETGNFGPLAGGLRLLGLWSGTVPDWSEIAEVDLTYFAGRF
SSARAEAMVTKDFTDVDALKSALTAAGVEVRMSKDGVELVPGPTFDPAKLEDL
>Mature_453_residues
MVEIHLKNSKSRKVELLQPIEPGRVSLYLCGPTVYDRAHLGNARSAVVFDQLFRLLRHVYGAENVTFVRNFTDVDDKINA
RALAEGREIADLTAETSQWYLDDTRALGVLDPNHMPRATEYIAEMVAFIEDLIAKGFAYARDGHALFRVRSFDDYGKLSG
RSVDDMIAGARVEVAPYKEDPMDFVLWKPSSDDIPGWDSPWGRGRPGWHIECSAMAHALLGEQFDIHGGGADLMFPHHEN
EVAQSCAAGHGFANIWMHNEMLQVEGKKMSKSLGNFFTVRDLLDQGVPGEVIRFVMLSTHYGKPMDWTEKKRAEAEATLR
KWYAALRQSVAEQEATPHLVDSLADDLNTALAITTLHGMYETGNFGPLAGGLRLLGLWSGTVPDWSEIAEVDLTYFAGRF
SSARAEAMVTKDFTDVDALKSALTAAGVEVRMSKDGVELVPGPTFDPAKLEDL

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=314, Percent_Identity=42.3566878980892, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI62240992, Length=221, Percent_Identity=39.8190045248869, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI10835051, Length=221, Percent_Identity=39.8190045248869, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI303304987, Length=221, Percent_Identity=39.8190045248869, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI21269877, Length=221, Percent_Identity=39.8190045248869, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1786737, Length=415, Percent_Identity=42.8915662650602, Blast_Score=312, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17509797, Length=242, Percent_Identity=35.9504132231405, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17509795, Length=242, Percent_Identity=35.9504132231405, Blast_Score=159, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324082, Length=312, Percent_Identity=34.6153846153846, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI19922218, Length=379, Percent_Identity=36.1477572559367, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI19922364, Length=228, Percent_Identity=39.4736842105263, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_JANSC (Q28QY7)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509900.1
- ProteinModelPortal:   Q28QY7
- SMR:   Q28QY7
- STRING:   Q28QY7
- GeneID:   3934409
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1958
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   INARAAC
- BioCyc:   JSP290400:JANN_1958-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 50235; Mature: 50235

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEIHLKNSKSRKVELLQPIEPGRVSLYLCGPTVYDRAHLGNARSAVVFDQLFRLLRHVY
CEEEEECCCCCCCCCEECCCCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
GAENVTFVRNFTDVDDKINARALAEGREIADLTAETSQWYLDDTRALGVLDPNHMPRATE
CCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHCCCCEEECCCCEEECCCCCCCCHHHH
YIAEMVAFIEDLIAKGFAYARDGHALFRVRSFDDYGKLSGRSVDDMIAGARVEVAPYKED
HHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCCCCCHHHHHCCCEEEECCCCCC
PMDFVLWKPSSDDIPGWDSPWGRGRPGWHIECSAMAHALLGEQFDIHGGGADLMFPHHEN
CCCEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCEEECCCCCCEECCCCHH
EVAQSCAAGHGFANIWMHNEMLQVEGKKMSKSLGNFFTVRDLLDQGVPGEVIRFVMLSTH
HHHHHHHCCCCEEEEEEECCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
YGKPMDWTEKKRAEAEATLRKWYAALRQSVAEQEATPHLVDSLADDLNTALAITTLHGMY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ETGNFGPLAGGLRLLGLWSGTVPDWSEIAEVDLTYFAGRFSSARAEAMVTKDFTDVDALK
CCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHEEEECCCCHHHHHH
SALTAAGVEVRMSKDGVELVPGPTFDPAKLEDL
HHHHHCCEEEEECCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MVEIHLKNSKSRKVELLQPIEPGRVSLYLCGPTVYDRAHLGNARSAVVFDQLFRLLRHVY
CEEEEECCCCCCCCCEECCCCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
GAENVTFVRNFTDVDDKINARALAEGREIADLTAETSQWYLDDTRALGVLDPNHMPRATE
CCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHCCCCEEECCCCEEECCCCCCCCHHHH
YIAEMVAFIEDLIAKGFAYARDGHALFRVRSFDDYGKLSGRSVDDMIAGARVEVAPYKED
HHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCCCCCHHHHHCCCEEEECCCCCC
PMDFVLWKPSSDDIPGWDSPWGRGRPGWHIECSAMAHALLGEQFDIHGGGADLMFPHHEN
CCCEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCEEECCCCCCEECCCCHH
EVAQSCAAGHGFANIWMHNEMLQVEGKKMSKSLGNFFTVRDLLDQGVPGEVIRFVMLSTH
HHHHHHHCCCCEEEEEEECCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
YGKPMDWTEKKRAEAEATLRKWYAALRQSVAEQEATPHLVDSLADDLNTALAITTLHGMY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ETGNFGPLAGGLRLLGLWSGTVPDWSEIAEVDLTYFAGRFSSARAEAMVTKDFTDVDALK
CCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHEEEECCCCHHHHHH
SALTAAGVEVRMSKDGVELVPGPTFDPAKLEDL
HHHHHCCEEEEECCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA