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The map label for this gene is dctD [H]

Identifier: 89054221

GI number: 89054221

Start: 1721642

End: 1722985

Strand: Reverse

Name: dctD [H]

Synonym: Jann_1730

Alternate gene names: 89054221

Gene position: 1722985-1721642 (Counterclockwise)

Preceding gene: 89054222

Following gene: 89054220

Centisome position: 39.9

GC content: 66.44

Gene sequence:

>1344_bases
ATGACCCAAACGCCCGTCCTTTTCGTCGATGATGAGCCTGATCTGCGGATCGCGGGCGAGCAGACCCTGTCACTGGCCGA
TCTGCCGGTCATTGCCTGCGCCCGGGCTGAGGCCGCCCTGGATCACATTTCGCCCGCCTTTCCCGGGGTGCTGGTCACAG
ATATCCGGATGCCCGGCATGGACGGAACCGCCCTGATGGCCGAAGCGCTCAGGCGCGACCCGGATCTGCCAGTGATATTG
GTGACGGGGCACGGTGATGTGGACCTGGCGGTGCACAGCATGCGGCTGGGGGCTTATGACTTCATCGAAAAACCCTACGC
GCCGGACCGATTGGTGCAAGTGATCCGCCGCGCGCTGGACAAGCGCCGCCTGACGATGGAGGTGCGCGCCCTGAAGGCGC
AGGCCGCAGATGGCAGCGCGGATCCGATCTCCGCACAACTTATCGGGCGCTCCACCGCCATGGCGCAGCTGCGCGCGCAA
TTGAAGGCGGTGGCCGAGACGGAAACCGATGTGTTGATCGAAGGGGCCACCGGCACGGGCAAGGAGGTGGCGGCGCGCGC
GCTCCACGCGGCCTCGGCCCGGGCGGCCAGGCCCTTTGTCGCGATCAATTGCGCCGCCCTGCCCGAGACGCTGATGGAAA
GTGAGCTGTTTGGCCACGCTCCAGGTGCCTTTGAAGGCGCCACACGGGAGCGTTACGGCAAGTTCCAGCACGCCCGCGGT
GGCACCCTGTTTCTGGATCAGATCGACAGCATCCCCCTGCCCCTGCAAGGCAAATTGCTGACCGCCCTGCAGGACCGCGC
GATCACCCCCCTGGGCACGAATGAAGCCGTTGCCCTTGATCTGCGCGTCATCGCGGCCTCCAGACGCGATCTGGCGCAAG
CGGCGGCAGAGGGCGCGTTTCGGGATGATCTGCTCTACCGCCTCAACGTTGTGACCCTGCGGATGCCGCCGCTGGCGGAG
CGGCGCGAAGACATTCCCGGCCTCTTCCAGATCCTGCTGGCCAATGCGTCCGCCCGGCACAACCGTCCGGTGCCTGCGGT
CTCGCCCGATATGCTGGCAGATCTCGCCTCACGCGACTGGCCCGGCAATATCCGCGAGCTGCGCAATGAGGCCGAGCGCG
CGATCCTGGGGCTGGGACATGGCGGCGCGGCGTCGGAGACGGGACGGCTTGCCGATCAGATGGCCGCCCATGAACGCAGC
CTGATTGCCGCTACACTCAGCGCCCAGAACGGCTCGATCAAGGCGACATATGAGGCGCTGGGGATCAGCCGCAAAGCCCT
CTACGAGAAGATGCAGAAACACGGGCTTGCGCGCGACGACTTTCGTCCCGCCGACCGCGCGTAA

Upstream 100 bases:

>100_bases
CGGCCGAGAACCACGCCGGGGGCGGCGCGATCTTCCGCGTCACGCTGGAGGCCGCCACGCCTCAGGCCCCGGGTCTTGAA
GCACGGGAGCTGGCCGCCGA

Downstream 100 bases:

>100_bases
AACCCTTGCAAGGGTTTTACCAGAGCCTTGCAAGGCTCTGGACCATTGTCTTGCAAGACAATGGTTCCCGACCGATGGGT
CAATTTCCACCCACGACGGC

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTQTPVLFVDDEPDLRIAGEQTLSLADLPVIACARAEAALDHISPAFPGVLVTDIRMPGMDGTALMAEALRRDPDLPVIL
VTGHGDVDLAVHSMRLGAYDFIEKPYAPDRLVQVIRRALDKRRLTMEVRALKAQAADGSADPISAQLIGRSTAMAQLRAQ
LKAVAETETDVLIEGATGTGKEVAARALHAASARAARPFVAINCAALPETLMESELFGHAPGAFEGATRERYGKFQHARG
GTLFLDQIDSIPLPLQGKLLTALQDRAITPLGTNEAVALDLRVIAASRRDLAQAAAEGAFRDDLLYRLNVVTLRMPPLAE
RREDIPGLFQILLANASARHNRPVPAVSPDMLADLASRDWPGNIRELRNEAERAILGLGHGGAASETGRLADQMAAHERS
LIAATLSAQNGSIKATYEALGISRKALYEKMQKHGLARDDFRPADRA

Sequences:

>Translated_447_residues
MTQTPVLFVDDEPDLRIAGEQTLSLADLPVIACARAEAALDHISPAFPGVLVTDIRMPGMDGTALMAEALRRDPDLPVIL
VTGHGDVDLAVHSMRLGAYDFIEKPYAPDRLVQVIRRALDKRRLTMEVRALKAQAADGSADPISAQLIGRSTAMAQLRAQ
LKAVAETETDVLIEGATGTGKEVAARALHAASARAARPFVAINCAALPETLMESELFGHAPGAFEGATRERYGKFQHARG
GTLFLDQIDSIPLPLQGKLLTALQDRAITPLGTNEAVALDLRVIAASRRDLAQAAAEGAFRDDLLYRLNVVTLRMPPLAE
RREDIPGLFQILLANASARHNRPVPAVSPDMLADLASRDWPGNIRELRNEAERAILGLGHGGAASETGRLADQMAAHERS
LIAATLSAQNGSIKATYEALGISRKALYEKMQKHGLARDDFRPADRA
>Mature_446_residues
TQTPVLFVDDEPDLRIAGEQTLSLADLPVIACARAEAALDHISPAFPGVLVTDIRMPGMDGTALMAEALRRDPDLPVILV
TGHGDVDLAVHSMRLGAYDFIEKPYAPDRLVQVIRRALDKRRLTMEVRALKAQAADGSADPISAQLIGRSTAMAQLRAQL
KAVAETETDVLIEGATGTGKEVAARALHAASARAARPFVAINCAALPETLMESELFGHAPGAFEGATRERYGKFQHARGG
TLFLDQIDSIPLPLQGKLLTALQDRAITPLGTNEAVALDLRVIAASRRDLAQAAAEGAFRDDLLYRLNVVTLRMPPLAER
REDIPGLFQILLANASARHNRPVPAVSPDMLADLASRDWPGNIRELRNEAERAILGLGHGGAASETGRLADQMAAHERSL
IAATLSAQNGSIKATYEALGISRKALYEKMQKHGLARDDFRPADRA

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=453, Percent_Identity=38.4105960264901, Blast_Score=301, Evalue=8e-83,
Organism=Escherichia coli, GI1788905, Length=459, Percent_Identity=38.562091503268, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1790437, Length=413, Percent_Identity=38.7409200968523, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1790299, Length=369, Percent_Identity=40.1084010840108, Blast_Score=270, Evalue=9e-74,
Organism=Escherichia coli, GI87082117, Length=320, Percent_Identity=42.5, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1789233, Length=331, Percent_Identity=38.9728096676737, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1789087, Length=335, Percent_Identity=40.2985074626866, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87082152, Length=248, Percent_Identity=45.1612903225806, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786524, Length=351, Percent_Identity=36.4672364672365, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=34.6625766871166, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=38.0952380952381, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI1789828, Length=251, Percent_Identity=34.2629482071713, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=30.976430976431, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48056; Mature: 47924

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTPVLFVDDEPDLRIAGEQTLSLADLPVIACARAEAALDHISPAFPGVLVTDIRMPGM
CCCCCEEEECCCCCEEECCCCCEEHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
DGTALMAEALRRDPDLPVILVTGHGDVDLAVHSMRLGAYDFIEKPYAPDRLVQVIRRALD
CHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEHHHCCHHHHHHCCCCHHHHHHHHHHHHH
KRRLTMEVRALKAQAADGSADPISAQLIGRSTAMAQLRAQLKAVAETETDVLIEGATGTG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
KEVAARALHAASARAARPFVAINCAALPETLMESELFGHAPGAFEGATRERYGKFQHARG
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHCCCC
GTLFLDQIDSIPLPLQGKLLTALQDRAITPLGTNEAVALDLRVIAASRRDLAQAAAEGAF
CEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHCCCH
RDDLLYRLNVVTLRMPPLAERREDIPGLFQILLANASARHNRPVPAVSPDMLADLASRDW
HHHHHEEEEEEEEECCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCC
PGNIRELRNEAERAILGLGHGGAASETGRLADQMAAHERSLIAATLSAQNGSIKATYEAL
CCHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEHHHH
GISRKALYEKMQKHGLARDDFRPADRA
CCCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQTPVLFVDDEPDLRIAGEQTLSLADLPVIACARAEAALDHISPAFPGVLVTDIRMPGM
CCCCEEEECCCCCEEECCCCCEEHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
DGTALMAEALRRDPDLPVILVTGHGDVDLAVHSMRLGAYDFIEKPYAPDRLVQVIRRALD
CHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEHHHCCHHHHHHCCCCHHHHHHHHHHHHH
KRRLTMEVRALKAQAADGSADPISAQLIGRSTAMAQLRAQLKAVAETETDVLIEGATGTG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
KEVAARALHAASARAARPFVAINCAALPETLMESELFGHAPGAFEGATRERYGKFQHARG
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHCCCC
GTLFLDQIDSIPLPLQGKLLTALQDRAITPLGTNEAVALDLRVIAASRRDLAQAAAEGAF
CEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHCCCH
RDDLLYRLNVVTLRMPPLAERREDIPGLFQILLANASARHNRPVPAVSPDMLADLASRDW
HHHHHEEEEEEEEECCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCC
PGNIRELRNEAERAILGLGHGGAASETGRLADQMAAHERSLIAATLSAQNGSIKATYEAL
CCHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEHHHH
GISRKALYEKMQKHGLARDDFRPADRA
CCCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]