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The map label for this gene is caiC [H]

Identifier: 89053825

GI number: 89053825

Start: 1299472

End: 1301088

Strand: Direct

Name: caiC [H]

Synonym: Jann_1334

Alternate gene names: 89053825

Gene position: 1299472-1301088 (Clockwise)

Preceding gene: 89053824

Following gene: 89053826

Centisome position: 30.09

GC content: 62.28

Gene sequence:

>1617_bases
ATGACCAAGCCGCGAACCCAAACGTCCGTCTTTGACGCTTTCGAGGCCACCGCTATTGCGCATCCCGCGCGGGCCTTCCT
CAATGTCTTGCCCGCCACCGCTGCGATTTTTGACATCCCGGAAGGGGAAATCACGTATGGTGCAACGCTTTCTGACGTGA
GCGACATGGCGCAAGCGTTTGAAAAGGCCGGATACGCGGCAGGGATGCGGGTCGCCCTGCTTCTCGAAAACCGCCCCACG
TTCTTCATCATCTGGCTGGCGCTCAACAAAATTGGCGCGTCTGTCGTTCCGATCAACCCGGACCTGCGCGCGACGGAACT
GGAATACCTGATCGGCCATTCCGAGCCTGCCCTGATCGTCGCCATCCCGTCGCGCCACGCGGACCTGATTGCAGCGGCCA
AAGCGGCAGGTATCACGCTGGATGTGGTGGCACCTGGAGCGGCTTTGCCAGCGCCACGCAGCAACGCAATCGTCGCAAGG
GCGTTTGACGGAGACGCGCGGGAGGCGGCGATCCTTTACACCTCCGGTACGACGGGGAAGCCCAAGGGCTGCGTTCTGGC
GAACGCGTATTTCCTGCTGGCCGGGCGTTGGTATGCGGATCTTGGCGGCATCGCCACGCTCCGCGATGAAGGGGAGCGGA
TGATTACACCGCTGCCTATTTTTCACATGAACGCAATGGCTTATTCCTTCATGGCGATGGTGACCGTCGGCGGGTGTCTG
ACGGTGCTCGACCGCTTCCACCCACGCAGTTGGTGGGCTGATGTGAAGGCGTCGGGGGCGACGTGCCTGCATTACCTGGG
TGTGGTGCCAACGATGTTGATGGGGTTTGAGCCGTCGGGCGCGGACCGCGATCATGCTGTTCGGTTCGGTTTTGGCGCAG
GCGTGGACCCCAAATTGCAGGCAGAATTCGAGGCGCGCTTTGGCTTTCCCTTGGTCGAGGCTTGGGCGATGACCGAGACG
GGGGCGGGGGCGGTGATTGCCTCGGCCAAGGAGGATCGCCTGGTGGGGCAGGCCAGCATCGGCAAGCCGGACGCGGACGT
CGAATGCCGGCTGATTGATGACGCAGGCAATGATGCGCGACAGGGAGAGTTGCTGGTGCGGCGGGCGGGCGCTGACCCGC
GCTACGGTTTCTTTTCCGAGTATTACAAGAACCAGCAGGCGACGGATGAGGCCTGGGCTGACGGCTGGTTCCACACCGGC
GACATCGTGCGCAAAGACGCGGCCGGGACCTACTCCTTCGTGGATCGCAAGAAGAACGTCATCCGGAGGTCCGGCGAGAA
CATCGCGGCGGTTGAGGTGGAATCTATCCTTATGCGGCATCCGCGCGTCACCGCGGTGGGCGTGGCCCCGGTTCCTGATC
CGATCCGAGGCGACGAAGTCTTCGCCTGCATGACGGTGGACAATCCAAGCCCCGAATTGGCGCTGGAGATTACGGAATGG
TGCCTGGCGCAGATGGCCTATTACAAAGCGCCGGGCTACATCGCCTTTGTGGATGGGCTGCCATTGACCCCAACGCAGAA
GATCCAGCGGACCGTCCTAAAAGACCTTGCAGGTCGCCTCACGACGGACCCGCAAACCGTAGTGACCGCGCACCTCAAGA
AACGGCAGGCGAATTGA

Upstream 100 bases:

>100_bases
CTGGATCAAACGGCTGCGGATAGCGGCGCTGGAAGACGAACACGGCGACGCGCTGGACGGGGCGCTTGCAACCGTGCGCT
CGTTTGCTTGAGGTAGACCT

Downstream 100 bases:

>100_bases
TGACCCGAAGACAGGACTATTCGGGTATCGCAATCACCGCGCCGTTTTCAATGGCCTACCAGCGGTATTCGATTGATACG
GCCCATTGGTGGATCGCCAA

Product: AMP-dependent synthetase and ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTKPRTQTSVFDAFEATAIAHPARAFLNVLPATAAIFDIPEGEITYGATLSDVSDMAQAFEKAGYAAGMRVALLLENRPT
FFIIWLALNKIGASVVPINPDLRATELEYLIGHSEPALIVAIPSRHADLIAAAKAAGITLDVVAPGAALPAPRSNAIVAR
AFDGDAREAAILYTSGTTGKPKGCVLANAYFLLAGRWYADLGGIATLRDEGERMITPLPIFHMNAMAYSFMAMVTVGGCL
TVLDRFHPRSWWADVKASGATCLHYLGVVPTMLMGFEPSGADRDHAVRFGFGAGVDPKLQAEFEARFGFPLVEAWAMTET
GAGAVIASAKEDRLVGQASIGKPDADVECRLIDDAGNDARQGELLVRRAGADPRYGFFSEYYKNQQATDEAWADGWFHTG
DIVRKDAAGTYSFVDRKKNVIRRSGENIAAVEVESILMRHPRVTAVGVAPVPDPIRGDEVFACMTVDNPSPELALEITEW
CLAQMAYYKAPGYIAFVDGLPLTPTQKIQRTVLKDLAGRLTTDPQTVVTAHLKKRQAN

Sequences:

>Translated_538_residues
MTKPRTQTSVFDAFEATAIAHPARAFLNVLPATAAIFDIPEGEITYGATLSDVSDMAQAFEKAGYAAGMRVALLLENRPT
FFIIWLALNKIGASVVPINPDLRATELEYLIGHSEPALIVAIPSRHADLIAAAKAAGITLDVVAPGAALPAPRSNAIVAR
AFDGDAREAAILYTSGTTGKPKGCVLANAYFLLAGRWYADLGGIATLRDEGERMITPLPIFHMNAMAYSFMAMVTVGGCL
TVLDRFHPRSWWADVKASGATCLHYLGVVPTMLMGFEPSGADRDHAVRFGFGAGVDPKLQAEFEARFGFPLVEAWAMTET
GAGAVIASAKEDRLVGQASIGKPDADVECRLIDDAGNDARQGELLVRRAGADPRYGFFSEYYKNQQATDEAWADGWFHTG
DIVRKDAAGTYSFVDRKKNVIRRSGENIAAVEVESILMRHPRVTAVGVAPVPDPIRGDEVFACMTVDNPSPELALEITEW
CLAQMAYYKAPGYIAFVDGLPLTPTQKIQRTVLKDLAGRLTTDPQTVVTAHLKKRQAN
>Mature_537_residues
TKPRTQTSVFDAFEATAIAHPARAFLNVLPATAAIFDIPEGEITYGATLSDVSDMAQAFEKAGYAAGMRVALLLENRPTF
FIIWLALNKIGASVVPINPDLRATELEYLIGHSEPALIVAIPSRHADLIAAAKAAGITLDVVAPGAALPAPRSNAIVARA
FDGDAREAAILYTSGTTGKPKGCVLANAYFLLAGRWYADLGGIATLRDEGERMITPLPIFHMNAMAYSFMAMVTVGGCLT
VLDRFHPRSWWADVKASGATCLHYLGVVPTMLMGFEPSGADRDHAVRFGFGAGVDPKLQAEFEARFGFPLVEAWAMTETG
AGAVIASAKEDRLVGQASIGKPDADVECRLIDDAGNDARQGELLVRRAGADPRYGFFSEYYKNQQATDEAWADGWFHTGD
IVRKDAAGTYSFVDRKKNVIRRSGENIAAVEVESILMRHPRVTAVGVAPVPDPIRGDEVFACMTVDNPSPELALEITEWC
LAQMAYYKAPGYIAFVDGLPLTPTQKIQRTVLKDLAGRLTTDPQTVVTAHLKKRQAN

Specific function: Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=562, Percent_Identity=25.0889679715303, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI187761345, Length=493, Percent_Identity=26.369168356998, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI187761343, Length=493, Percent_Identity=26.369168356998, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI62865631, Length=546, Percent_Identity=24.9084249084249, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI13325055, Length=546, Percent_Identity=24.9084249084249, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI122937307, Length=490, Percent_Identity=26.9387755102041, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI157311624, Length=490, Percent_Identity=25.1020408163265, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157311622, Length=490, Percent_Identity=25.1020408163265, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI38505220, Length=485, Percent_Identity=26.5979381443299, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI38524616, Length=542, Percent_Identity=27.3062730627306, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI40807357, Length=545, Percent_Identity=24.7706422018349, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI58082049, Length=490, Percent_Identity=24.8979591836735, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI13325057, Length=545, Percent_Identity=24.2201834862385, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI13236579, Length=488, Percent_Identity=25.2049180327869, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI42544132, Length=480, Percent_Identity=24.375, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI227499619, Length=471, Percent_Identity=24.416135881104, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI115511026, Length=489, Percent_Identity=24.9488752556237, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI253970489, Length=173, Percent_Identity=27.7456647398844, Blast_Score=66, Evalue=8e-11,
Organism=Homo sapiens, GI8923896, Length=173, Percent_Identity=27.7456647398844, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI221142682, Length=489, Percent_Identity=30.4703476482618, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI145693145, Length=521, Percent_Identity=30.1343570057582, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788107, Length=569, Percent_Identity=26.3620386643234, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1790505, Length=373, Percent_Identity=24.6648793565684, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786810, Length=508, Percent_Identity=25.3937007874016, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788595, Length=516, Percent_Identity=24.6124031007752, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=526, Percent_Identity=25.8555133079848, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17551278, Length=546, Percent_Identity=24.7252747252747, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=500, Percent_Identity=24.6, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=550, Percent_Identity=23.6363636363636, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=550, Percent_Identity=23.6363636363636, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=550, Percent_Identity=23.6363636363636, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115534440, Length=469, Percent_Identity=23.454157782516, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=363, Percent_Identity=25.8953168044077, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=500, Percent_Identity=23.4, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=501, Percent_Identity=24.9500998003992, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=23.1638418079096, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17510401, Length=330, Percent_Identity=28.1818181818182, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=371, Percent_Identity=23.7196765498652, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=529, Percent_Identity=25.1417769376182, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6319515, Length=492, Percent_Identity=22.9674796747967, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324893, Length=336, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28573631, Length=528, Percent_Identity=25.7575757575758, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI20130353, Length=528, Percent_Identity=25.7575757575758, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45550496, Length=570, Percent_Identity=26.140350877193, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI45552801, Length=517, Percent_Identity=27.4661508704062, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24583465, Length=555, Percent_Identity=24.8648648648649, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583463, Length=555, Percent_Identity=24.8648648648649, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281364798, Length=555, Percent_Identity=24.8648648648649, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24581924, Length=524, Percent_Identity=24.4274809160305, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18859661, Length=479, Percent_Identity=27.1398747390397, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21355181, Length=521, Percent_Identity=21.8809980806142, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24667959, Length=365, Percent_Identity=24.6575342465753, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62472339, Length=365, Percent_Identity=24.6575342465753, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24667955, Length=365, Percent_Identity=24.6575342465753, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648260, Length=366, Percent_Identity=23.7704918032787, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 58020; Mature: 57889

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPRTQTSVFDAFEATAIAHPARAFLNVLPATAAIFDIPEGEITYGATLSDVSDMAQAF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEECCCHHHHHHHHHHH
EKAGYAAGMRVALLLENRPTFFIIWLALNKIGASVVPINPDLRATELEYLIGHSEPALIV
HHCCCCCCCEEEEEEECCCCEEEEEEEHHHCCCEEEECCCCCCHHHHHHEECCCCCEEEE
AIPSRHADLIAAAKAAGITLDVVAPGAALPAPRSNAIVARAFDGDAREAAILYTSGTTGK
EECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCC
PKGCVLANAYFLLAGRWYADLGGIATLRDEGERMITPLPIFHMNAMAYSFMAMVTVGGCL
CCCEEEEEEEEEEECCHHHHCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
TVLDRFHPRSWWADVKASGATCLHYLGVVPTMLMGFEPSGADRDHAVRFGFGAGVDPKLQ
HHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEE
AEFEARFGFPLVEAWAMTETGAGAVIASAKEDRLVGQASIGKPDADVECRLIDDAGNDAR
EEEHHHCCCHHHHHHEECCCCCCEEEECCCCCCEEEECCCCCCCCCEEEEEEECCCCCCC
QGELLVRRAGADPRYGFFSEYYKNQQATDEAWADGWFHTGDIVRKDAAGTYSFVDRKKNV
CCCEEEEECCCCCCCCHHHHHHCCCCCCHHHHHCCCEECCCHHCCCCCCCHHHHHHHHHH
IRRSGENIAAVEVESILMRHPRVTAVGVAPVPDPIRGDEVFACMTVDNPSPELALEITEW
HHHCCCCEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEHHHHHH
CLAQMAYYKAPGYIAFVDGLPLTPTQKIQRTVLKDLAGRLTTDPQTVVTAHLKKRQAN
HHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKPRTQTSVFDAFEATAIAHPARAFLNVLPATAAIFDIPEGEITYGATLSDVSDMAQAF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEECCCHHHHHHHHHHH
EKAGYAAGMRVALLLENRPTFFIIWLALNKIGASVVPINPDLRATELEYLIGHSEPALIV
HHCCCCCCCEEEEEEECCCCEEEEEEEHHHCCCEEEECCCCCCHHHHHHEECCCCCEEEE
AIPSRHADLIAAAKAAGITLDVVAPGAALPAPRSNAIVARAFDGDAREAAILYTSGTTGK
EECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCC
PKGCVLANAYFLLAGRWYADLGGIATLRDEGERMITPLPIFHMNAMAYSFMAMVTVGGCL
CCCEEEEEEEEEEECCHHHHCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
TVLDRFHPRSWWADVKASGATCLHYLGVVPTMLMGFEPSGADRDHAVRFGFGAGVDPKLQ
HHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEE
AEFEARFGFPLVEAWAMTETGAGAVIASAKEDRLVGQASIGKPDADVECRLIDDAGNDAR
EEEHHHCCCHHHHHHEECCCCCCEEEECCCCCCEEEECCCCCCCCCEEEEEEECCCCCCC
QGELLVRRAGADPRYGFFSEYYKNQQATDEAWADGWFHTGDIVRKDAAGTYSFVDRKKNV
CCCEEEEECCCCCCCCHHHHHHCCCCCCHHHHHCCCEECCCHHCCCCCCCHHHHHHHHHH
IRRSGENIAAVEVESILMRHPRVTAVGVAPVPDPIRGDEVFACMTVDNPSPELALEITEW
HHHCCCCEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEHHHHHH
CLAQMAYYKAPGYIAFVDGLPLTPTQKIQRTVLKDLAGRLTTDPQTVVTAHLKKRQAN
HHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA