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The map label for this gene is mltB [H]

Identifier: 89053649

GI number: 89053649

Start: 1121833

End: 1122654

Strand: Direct

Name: mltB [H]

Synonym: Jann_1158

Alternate gene names: 89053649

Gene position: 1121833-1122654 (Clockwise)

Preceding gene: 89053646

Following gene: 89053654

Centisome position: 25.98

GC content: 60.83

Gene sequence:

>822_bases
ATGCACACACGCCTGACCCGCGCCCTTCGCTGCGCCATAGTGACGTCCCTCTTGCTCGGCGCACCTATGGCGGCCGTGGC
TGCCACCTGCGGCAATGACGCCTCCGGTTTCGCCCGCTGGCAGCAGGAATTCGCAGCGGAAGCCGCCGCCGCAGGTGTCG
GTCAAGCCGGGTTGCAAGCTCTGGCCGCCACGACTTATGCCACGCGCACGATCTCGGCAGACCGCAATCAGCGCTCCTTC
AGTTATTCCCTCGAAGAGTTCATGCGAATTCGTGGTGCCGATGCGATTGTGCGGCAAGGTCGGCAGCGGATCGCTGCAAA
CCCTGAATTTTACGCCTCGCTCGAGCGTGCTTACGGCGTACCCGCGGCAGTGATCGTCGCTATTCACGGAATGGAAACCG
GTTTTGGCAATTTCATGGGCGATGCAAACGTCGTCTCGGCCATTGCAACGCTTGCCTATGACTGCCGCCGCTCGGCCTTT
TTCAGCGAACATGCGTTGGCCGCGCTCATTCTCGTGGATCGCGGCGCAATCAGCCCCAATTCAATCGGCGCGATGCATGG
TGAACTCGGGCATACGCAATTCTTGCCGGGCAACGTTCTGCGCTACGGCGTAGATGGCAATGGCGACGGGCGCATAGATC
TGAGTAATTTTACCGATGCCATGGCGTCGACCGCGAACTTTCTGCGCCAGAACGGCTGGCGGCAAGGCCGAGGCTACCAG
GAAGGCCAACGCAATTTTAGGGCAATCCAAGCGTGGAATGCGGCGCCGGTCTATCAGCGCGCCATTGCGATCATGGCGAG
CCGGATAGAAGGCCAGTCCTAA

Upstream 100 bases:

>100_bases
CTTTGCATTCGCGAAACGATATTTTGCCAACGCGCGCGTCTCTCATGCTAGACTTTTCGCAATTCGGCTAAAGTCATCCC
GTCGCCTTCGGAGATAAATC

Downstream 100 bases:

>100_bases
CCACGGACAGGACATGAAGAGGCCAGTGCATCCGCCAATTTAGGCCCAAACCGGACCTTCATCATGTTGGCGAATGCTAA
AATGCGGCTTCCCCAAAGCA

Product: hypothetical protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MHTRLTRALRCAIVTSLLLGAPMAAVAATCGNDASGFARWQQEFAAEAAAAGVGQAGLQALAATTYATRTISADRNQRSF
SYSLEEFMRIRGADAIVRQGRQRIAANPEFYASLERAYGVPAAVIVAIHGMETGFGNFMGDANVVSAIATLAYDCRRSAF
FSEHALAALILVDRGAISPNSIGAMHGELGHTQFLPGNVLRYGVDGNGDGRIDLSNFTDAMASTANFLRQNGWRQGRGYQ
EGQRNFRAIQAWNAAPVYQRAIAIMASRIEGQS

Sequences:

>Translated_273_residues
MHTRLTRALRCAIVTSLLLGAPMAAVAATCGNDASGFARWQQEFAAEAAAAGVGQAGLQALAATTYATRTISADRNQRSF
SYSLEEFMRIRGADAIVRQGRQRIAANPEFYASLERAYGVPAAVIVAIHGMETGFGNFMGDANVVSAIATLAYDCRRSAF
FSEHALAALILVDRGAISPNSIGAMHGELGHTQFLPGNVLRYGVDGNGDGRIDLSNFTDAMASTANFLRQNGWRQGRGYQ
EGQRNFRAIQAWNAAPVYQRAIAIMASRIEGQS
>Mature_273_residues
MHTRLTRALRCAIVTSLLLGAPMAAVAATCGNDASGFARWQQEFAAEAAAAGVGQAGLQALAATTYATRTISADRNQRSF
SYSLEEFMRIRGADAIVRQGRQRIAANPEFYASLERAYGVPAAVIVAIHGMETGFGNFMGDANVVSAIATLAYDCRRSAF
FSEHALAALILVDRGAISPNSIGAMHGELGHTQFLPGNVLRYGVDGNGDGRIDLSNFTDAMASTANFLRQNGWRQGRGYQ
EGQRNFRAIQAWNAAPVYQRAIAIMASRIEGQS

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=123, Percent_Identity=37.3983739837398, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 29242; Mature: 29242

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTRLTRALRCAIVTSLLLGAPMAAVAATCGNDASGFARWQQEFAAEAAAAGVGQAGLQA
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
LAATTYATRTISADRNQRSFSYSLEEFMRIRGADAIVRQGRQRIAANPEFYASLERAYGV
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PAAVIVAIHGMETGFGNFMGDANVVSAIATLAYDCRRSAFFSEHALAALILVDRGAISPN
CHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
SIGAMHGELGHTQFLPGNVLRYGVDGNGDGRIDLSNFTDAMASTANFLRQNGWRQGRGYQ
CCHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCHH
EGQRNFRAIQAWNAAPVYQRAIAIMASRIEGQS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHTRLTRALRCAIVTSLLLGAPMAAVAATCGNDASGFARWQQEFAAEAAAAGVGQAGLQA
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
LAATTYATRTISADRNQRSFSYSLEEFMRIRGADAIVRQGRQRIAANPEFYASLERAYGV
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PAAVIVAIHGMETGFGNFMGDANVVSAIATLAYDCRRSAFFSEHALAALILVDRGAISPN
CHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
SIGAMHGELGHTQFLPGNVLRYGVDGNGDGRIDLSNFTDAMASTANFLRQNGWRQGRGYQ
CCHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCHH
EGQRNFRAIQAWNAAPVYQRAIAIMASRIEGQS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]