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The map label for this gene is gcvA [H]

Identifier: 89053570

GI number: 89053570

Start: 1041335

End: 1042228

Strand: Direct

Name: gcvA [H]

Synonym: Jann_1079

Alternate gene names: 89053570

Gene position: 1041335-1042228 (Clockwise)

Preceding gene: 89053563

Following gene: 89053572

Centisome position: 24.12

GC content: 62.3

Gene sequence:

>894_bases
GTGAAACTCGCGCGCCGTCTTCCCTCTCTGAATGCACTCCGGGCCCTGGAAGCTGTGCGGGAAACCGGATCTGTCTCTGC
CGCTGGGCGTCGGCTGAGTGTCTCCCACTCGGCTGTCAGCCATCAGATCAAGCAATTGGAGGAGTGGATCGGTCGCCCCA
TCACGGTGCGGCGAGGCCGGAGTGTGGCGTTGACGGAGGCCGGAGAAAGCCTTGCGCGCGTGGTCCACGATTCGTTCGAT
GCGATCCGCCACGAACTGGACCTCCTGCCCCTTCGGTTTGCCCGCGCCGTCTCGATTTCAGCGCTTCCCGTCATCGCAGA
GGAAATCCTGCTCCCCAATCTTGAAGCGTTCCGAAGCGCGTTCCCCACCATAAGTTTGCATGTGTCGCTGGCGCAAACGG
ACCGACCAAAAACACCTGCACCGGATGTGGAAATCCTGTTTCGCAAGCAAGACCGCCTGCTGCCGGATGAAGGGGTTTTG
TTTCCGGGGGACGCGGTTCCGGTCTGCACGCCCGCGCTGCTTGCGGCGGCGGGCAATGACCCCGCAAGAGCCGTTCGAAA
TGGCCCGCTTCTGACCGATGAGGATTCCCGGATGTGGACAAGGTGGCTCCCGCAGGCGGGCGACGAGTGGTCTCCGAGCG
AGTCGACCCAGACGATCTATCTTGAAGGCTCGTTCCTGCTGCAAAAGGCCGCCGAGCACGGGATCGGCATTGCCATTTGT
CGCAGGGCAACCATCGCCCGCGCGCTGCAAACCGGTGTGCTCGTGCTGCTGTCGGACATCGCGATTGATCACGACTGGGC
CTACACGCTGAGAGTTGCGACGGGTCGCGAGATTGAACCCGAGGTCCAGATTGTCACGGCCTGGCTGAACAGCCTTACAC
CGCTCAGGCAATAG

Upstream 100 bases:

>100_bases
ACCCTTTGATTGGTGCGACCCCCAACAATAGGGTGCGAAACCCTCAAGAAAAAAGAACAAAATTGTTGCAATCTGTCTGA
ATTTCTCACAGGTCGGATGG

Downstream 100 bases:

>100_bases
CTGTGTCACCGGTTTGGTGCATTGCACGCAAAGATGTGCCGCCGGTTGTCGCCATAGGAAAAGGCGGCCCGTGGTGGGGC
CGCCTGTCCGCGTTAACAGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKLARRLPSLNALRALEAVRETGSVSAAGRRLSVSHSAVSHQIKQLEEWIGRPITVRRGRSVALTEAGESLARVVHDSFD
AIRHELDLLPLRFARAVSISALPVIAEEILLPNLEAFRSAFPTISLHVSLAQTDRPKTPAPDVEILFRKQDRLLPDEGVL
FPGDAVPVCTPALLAAAGNDPARAVRNGPLLTDEDSRMWTRWLPQAGDEWSPSESTQTIYLEGSFLLQKAAEHGIGIAIC
RRATIARALQTGVLVLLSDIAIDHDWAYTLRVATGREIEPEVQIVTAWLNSLTPLRQ

Sequences:

>Translated_297_residues
MKLARRLPSLNALRALEAVRETGSVSAAGRRLSVSHSAVSHQIKQLEEWIGRPITVRRGRSVALTEAGESLARVVHDSFD
AIRHELDLLPLRFARAVSISALPVIAEEILLPNLEAFRSAFPTISLHVSLAQTDRPKTPAPDVEILFRKQDRLLPDEGVL
FPGDAVPVCTPALLAAAGNDPARAVRNGPLLTDEDSRMWTRWLPQAGDEWSPSESTQTIYLEGSFLLQKAAEHGIGIAIC
RRATIARALQTGVLVLLSDIAIDHDWAYTLRVATGREIEPEVQIVTAWLNSLTPLRQ
>Mature_297_residues
MKLARRLPSLNALRALEAVRETGSVSAAGRRLSVSHSAVSHQIKQLEEWIGRPITVRRGRSVALTEAGESLARVVHDSFD
AIRHELDLLPLRFARAVSISALPVIAEEILLPNLEAFRSAFPTISLHVSLAQTDRPKTPAPDVEILFRKQDRLLPDEGVL
FPGDAVPVCTPALLAAAGNDPARAVRNGPLLTDEDSRMWTRWLPQAGDEWSPSESTQTIYLEGSFLLQKAAEHGIGIAIC
RRATIARALQTGVLVLLSDIAIDHDWAYTLRVATGREIEPEVQIVTAWLNSLTPLRQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=273, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=26.2798634812287, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=27.4576271186441, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32593; Mature: 32593

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLARRLPSLNALRALEAVRETGSVSAAGRRLSVSHSAVSHQIKQLEEWIGRPITVRRGR
CCHHHHCCCHHHHHHHHHHHHCCCCHHCCCEEECHHHHHHHHHHHHHHHHCCCEEEECCC
SVALTEAGESLARVVHDSFDAIRHELDLLPLRFARAVSISALPVIAEEILLPNLEAFRSA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FPTISLHVSLAQTDRPKTPAPDVEILFRKQDRLLPDEGVLFPGDAVPVCTPALLAAAGND
CCEEEEEEEEECCCCCCCCCCCHHEEEEHHCCCCCCCCEECCCCCCCHHHHHHHHHCCCC
PARAVRNGPLLTDEDSRMWTRWLPQAGDEWSPSESTQTIYLEGSFLLQKAAEHGIGIAIC
HHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHH
RRATIARALQTGVLVLLSDIAIDHDWAYTLRVATGREIEPEVQIVTAWLNSLTPLRQ
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLARRLPSLNALRALEAVRETGSVSAAGRRLSVSHSAVSHQIKQLEEWIGRPITVRRGR
CCHHHHCCCHHHHHHHHHHHHCCCCHHCCCEEECHHHHHHHHHHHHHHHHCCCEEEECCC
SVALTEAGESLARVVHDSFDAIRHELDLLPLRFARAVSISALPVIAEEILLPNLEAFRSA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FPTISLHVSLAQTDRPKTPAPDVEILFRKQDRLLPDEGVLFPGDAVPVCTPALLAAAGND
CCEEEEEEEEECCCCCCCCCCCHHEEEEHHCCCCCCCCEECCCCCCCHHHHHHHHHCCCC
PARAVRNGPLLTDEDSRMWTRWLPQAGDEWSPSESTQTIYLEGSFLLQKAAEHGIGIAIC
HHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHH
RRATIARALQTGVLVLLSDIAIDHDWAYTLRVATGREIEPEVQIVTAWLNSLTPLRQ
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]