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The map label for this gene is priA [H]

Identifier: 89053524

GI number: 89053524

Start: 984955

End: 987153

Strand: Direct

Name: priA [H]

Synonym: Jann_1033

Alternate gene names: 89053524

Gene position: 984955-987153 (Clockwise)

Preceding gene: 89053519

Following gene: 89053525

Centisome position: 22.81

GC content: 65.48

Gene sequence:

>2199_bases
ATGGACCCCATGATTGAGCATTTTGATGAAGGCGATTTGGTTGGGGTTCTGACGGCAGAGCCCCTGGACCGCGTGCTTGA
TTACAAAGCGCCCGCAGGGGGGTGCTGGTTAGGCGCGTTTGTGGAAGTGCCGCTGGGTCCGCGCAAAGTTCTGGGCGTGG
TCTGGGGGCCGGGGCGGGGCGATTGGGACATTGCCAAGGTTCGGTCTGTCAACCGGGTGCTGGACGTGGCCCCGATGCGG
GCCGAGATGCGGAAGTTTCTGGAAAAAGCGGGGGCTTACACGCTGACGCCCATGTCGCTGATGCTGCGTCTGGCCACGCG
GGTGCCGGGCCTGAGCGATCCGCCGTCGATGCGCAAGGTCTACCGTCTGGGGCAGGGCACGCCGGACCGGGTGACGCCCG
CGCGCACCCGGGTGATCGAGGCGTTGGTGGAATATGGTGGCCTCGCCTTCACGTTGAAGGAGTTGAGCGAACTGGCCGGC
GTCACATCGACGGTCGTGAAGGGTCTTGCCAAGCAGGGCGTGGTGGAAGAGGCGGACACGCCCCGCGATGCCCCCTACCT
GACGCTCGACCCCGGCCTGCCCGGCAAGGAATTGACCGAGGATCAGGAGATTGCCGCCGATCGTTTGCAGGAGGGATCGC
GCGCCCGGCGCTATGGGGCGGTTCTGCTGAAAGGTGTGACCGGGTCCGGCAAGACGGAAGTGTATCTGGAGGCGGTCGCG
GAATGCCTGCGTCAGGGGCGTCAGGCGCTTGTACTGTTGCCGGAAATCGCGCTGACCTCTGAATTCCTGACCCGCGTGAA
GGCACGCTTCGGGGCCGATCCGGCGGAGTGGCATTCCGGCGTCACCATGACCGAACGCCGCCGCTGCTGGCGCATGGTCG
GGCAGGGGGAGGCACAGTTGGTCGTCGGCGCGCGATCCGCGCTGTTTCTGCCCTACCGGGACCTTGGGTTGATCGTCGTG
GATGAGGAACACGACACTTCCTACAAGCAGGACGAAGGGGCCAGCTACAACGCTCGCGATATGGCCGTTCTGCGCGCGTC
CCTGAACGAGGCGCAGGTGGTTTTGGCGTCTGCCACGCCGTCGTTGGAGACGTGGTCCAACGCGCAGGCGGGCAAATACA
CGCGCCTTGATCTGGTGTCCCGGTTCGGGCCCCAGGATCTGCCGGATATGGCATCCATCGACATGCGGTTGGAAGATCTG
CCGGGGGGCTCCTGGCTGTCACCTTCGCTGACATCCGCCGTAGGGGCGCGGTTGCAGGCGGGTGAACAGGCATTGTTGTT
CCTGAACCGTCGTGGCTACGCGCCCGTTACCCTGTGTCGCGCCTGCGGAGAGATGGTGGGCTGCGACCATTGTGACGCGC
GCATGGTGGAACATCGGTTCCAGAAACGCCTGATCTGCCACCAATGCGGCGAGACCAAACCCCTGCCGGAGGTCTGCCCC
TCCTGCGGGGCGGAGGGCAAGATGGCCGCGGTCGGCCCGGGTGTGGAGCGGATCGCGGAAGAAGCCGCCGCCGCCTTTCC
CGATGCGAAATTGGCGATCCTGTCGTCCGACCTGTTCGGCTCGGCCCGGGCGTTGAAAGCGCAGATTGAGGAACTGGCGA
ACGGCGATACCGACCTGATCATCGGCACGCAATTGGTGGCCAAAGGGCACAACTTCCCGCGCCTGACGCTGGTGGGTGTC
GTGGATGCGGATTTGGGCCTGCAAGGATCAGATTTGCGCGCGGCAGAGCGGACGTTCCAGCTGATGCGGCAGGTGGCGGG
GCGGGCCGGTCGTGCCGACAAGCCGGGCCAAGCGCTGTTGCAGACGTTCCAGCCCGAACATCCGGTGATCCGCGCAATCC
TGTCGGGCGATGAAGAGGGGTTCTGGCGTACGGAAGCAGCGGAGCGGGAGGCGGCTGGCATGCCGCCCTATGGTCGTCTC
GCGGGGATAATTATCTCCAGCACGGAAGCCGCCGCTGCCTTCGACCTCGGCACGCATCTGGCCCGCAACGATGGCCCGAT
CCGTGAGGCGGGCGGTATCGTCTATGGCCCGGCCCCAGCCCCCATCGCCCGTGTGCGCGGCAGGCACCGGGTGCGACTTC
TGGTCAAGGCCCCAAAGGGTGTCGCGCTGCAACCGGCCCTAAGCCGTTGGACCCACGGGCTAAAACTGCCCGGCCATCTG
CGACTTGCCATAGATATCGACCCGCAGAACTTCTTCTGA

Upstream 100 bases:

>100_bases
TCATCTGGCTGGTTCCTTTCAGCGGAAGATCATGGTGATTTGGCGCAGGCATATCGCATGTTAGCGCTAACGGAAACCCC
CAGTTTGCCGAGCCATTTGA

Downstream 100 bases:

>100_bases
AAACGGTTCCCCCCCGCCGTCGCGCACGGTAGAAGTTTGCGAAATCGTAAACGGACGCTTTGCCCATGACCGCTCCCATC
CGCCCGCAACCCGGCATTCT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MDPMIEHFDEGDLVGVLTAEPLDRVLDYKAPAGGCWLGAFVEVPLGPRKVLGVVWGPGRGDWDIAKVRSVNRVLDVAPMR
AEMRKFLEKAGAYTLTPMSLMLRLATRVPGLSDPPSMRKVYRLGQGTPDRVTPARTRVIEALVEYGGLAFTLKELSELAG
VTSTVVKGLAKQGVVEEADTPRDAPYLTLDPGLPGKELTEDQEIAADRLQEGSRARRYGAVLLKGVTGSGKTEVYLEAVA
ECLRQGRQALVLLPEIALTSEFLTRVKARFGADPAEWHSGVTMTERRRCWRMVGQGEAQLVVGARSALFLPYRDLGLIVV
DEEHDTSYKQDEGASYNARDMAVLRASLNEAQVVLASATPSLETWSNAQAGKYTRLDLVSRFGPQDLPDMASIDMRLEDL
PGGSWLSPSLTSAVGARLQAGEQALLFLNRRGYAPVTLCRACGEMVGCDHCDARMVEHRFQKRLICHQCGETKPLPEVCP
SCGAEGKMAAVGPGVERIAEEAAAAFPDAKLAILSSDLFGSARALKAQIEELANGDTDLIIGTQLVAKGHNFPRLTLVGV
VDADLGLQGSDLRAAERTFQLMRQVAGRAGRADKPGQALLQTFQPEHPVIRAILSGDEEGFWRTEAAEREAAGMPPYGRL
AGIIISSTEAAAAFDLGTHLARNDGPIREAGGIVYGPAPAPIARVRGRHRVRLLVKAPKGVALQPALSRWTHGLKLPGHL
RLAIDIDPQNFF

Sequences:

>Translated_732_residues
MDPMIEHFDEGDLVGVLTAEPLDRVLDYKAPAGGCWLGAFVEVPLGPRKVLGVVWGPGRGDWDIAKVRSVNRVLDVAPMR
AEMRKFLEKAGAYTLTPMSLMLRLATRVPGLSDPPSMRKVYRLGQGTPDRVTPARTRVIEALVEYGGLAFTLKELSELAG
VTSTVVKGLAKQGVVEEADTPRDAPYLTLDPGLPGKELTEDQEIAADRLQEGSRARRYGAVLLKGVTGSGKTEVYLEAVA
ECLRQGRQALVLLPEIALTSEFLTRVKARFGADPAEWHSGVTMTERRRCWRMVGQGEAQLVVGARSALFLPYRDLGLIVV
DEEHDTSYKQDEGASYNARDMAVLRASLNEAQVVLASATPSLETWSNAQAGKYTRLDLVSRFGPQDLPDMASIDMRLEDL
PGGSWLSPSLTSAVGARLQAGEQALLFLNRRGYAPVTLCRACGEMVGCDHCDARMVEHRFQKRLICHQCGETKPLPEVCP
SCGAEGKMAAVGPGVERIAEEAAAAFPDAKLAILSSDLFGSARALKAQIEELANGDTDLIIGTQLVAKGHNFPRLTLVGV
VDADLGLQGSDLRAAERTFQLMRQVAGRAGRADKPGQALLQTFQPEHPVIRAILSGDEEGFWRTEAAEREAAGMPPYGRL
AGIIISSTEAAAAFDLGTHLARNDGPIREAGGIVYGPAPAPIARVRGRHRVRLLVKAPKGVALQPALSRWTHGLKLPGHL
RLAIDIDPQNFF
>Mature_732_residues
MDPMIEHFDEGDLVGVLTAEPLDRVLDYKAPAGGCWLGAFVEVPLGPRKVLGVVWGPGRGDWDIAKVRSVNRVLDVAPMR
AEMRKFLEKAGAYTLTPMSLMLRLATRVPGLSDPPSMRKVYRLGQGTPDRVTPARTRVIEALVEYGGLAFTLKELSELAG
VTSTVVKGLAKQGVVEEADTPRDAPYLTLDPGLPGKELTEDQEIAADRLQEGSRARRYGAVLLKGVTGSGKTEVYLEAVA
ECLRQGRQALVLLPEIALTSEFLTRVKARFGADPAEWHSGVTMTERRRCWRMVGQGEAQLVVGARSALFLPYRDLGLIVV
DEEHDTSYKQDEGASYNARDMAVLRASLNEAQVVLASATPSLETWSNAQAGKYTRLDLVSRFGPQDLPDMASIDMRLEDL
PGGSWLSPSLTSAVGARLQAGEQALLFLNRRGYAPVTLCRACGEMVGCDHCDARMVEHRFQKRLICHQCGETKPLPEVCP
SCGAEGKMAAVGPGVERIAEEAAAAFPDAKLAILSSDLFGSARALKAQIEELANGDTDLIIGTQLVAKGHNFPRLTLVGV
VDADLGLQGSDLRAAERTFQLMRQVAGRAGRADKPGQALLQTFQPEHPVIRAILSGDEEGFWRTEAAEREAAGMPPYGRL
AGIIISSTEAAAAFDLGTHLARNDGPIREAGGIVYGPAPAPIARVRGRHRVRLLVKAPKGVALQPALSRWTHGLKLPGHL
RLAIDIDPQNFF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=705, Percent_Identity=35.3191489361702, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 79109; Mature: 79109

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPMIEHFDEGDLVGVLTAEPLDRVLDYKAPAGGCWLGAFVEVPLGPRKVLGVVWGPGRG
CCCHHHHCCCCCEEEEEECCHHHHHHCCCCCCCCEEEEHEEECCCCCCCEEEEEECCCCC
DWDIAKVRSVNRVLDVAPMRAEMRKFLEKAGAYTLTPMSLMLRLATRVPGLSDPPSMRKV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCCHHHHHH
YRLGQGTPDRVTPARTRVIEALVEYGGLAFTLKELSELAGVTSTVVKGLAKQGVVEEADT
HHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHCCCCCCCCC
PRDAPYLTLDPGLPGKELTEDQEIAADRLQEGSRARRYGAVLLKGVTGSGKTEVYLEAVA
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHCEEEEEECCCCCCHHHHHHHHH
ECLRQGRQALVLLPEIALTSEFLTRVKARFGADPAEWHSGVTMTERRRCWRMVGQGEAQL
HHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCEEE
VVGARSALFLPYRDLGLIVVDEEHDTSYKQDEGASYNARDMAVLRASLNEAQVVLASATP
EEECCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
SLETWSNAQAGKYTRLDLVSRFGPQDLPDMASIDMRLEDLPGGSWLSPSLTSAVGARLQA
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCEEECCCCCCCCCCHHHHHHHHHHHC
GEQALLFLNRRGYAPVTLCRACGEMVGCDHCDARMVEHRFQKRLICHQCGETKPLPEVCP
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
SCGAEGKMAAVGPGVERIAEEAAAAFPDAKLAILSSDLFGSARALKAQIEELANGDTDLI
CCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEE
IGTQLVAKGHNFPRLTLVGVVDADLGLQGSDLRAAERTFQLMRQVAGRAGRADKPGQALL
EEHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QTFQPEHPVIRAILSGDEEGFWRTEAAEREAAGMPPYGRLAGIIISSTEAAAAFDLGTHL
HHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHEEEEEECCCHHHHHHHHHHH
ARNDGPIREAGGIVYGPAPAPIARVRGRHRVRLLVKAPKGVALQPALSRWTHGLKLPGHL
HCCCCCHHHCCCEEECCCCCCHHHHCCCCEEEEEEECCCCCEECHHHHHHHCCCCCCCEE
RLAIDIDPQNFF
EEEEEECCCCCC
>Mature Secondary Structure
MDPMIEHFDEGDLVGVLTAEPLDRVLDYKAPAGGCWLGAFVEVPLGPRKVLGVVWGPGRG
CCCHHHHCCCCCEEEEEECCHHHHHHCCCCCCCCEEEEHEEECCCCCCCEEEEEECCCCC
DWDIAKVRSVNRVLDVAPMRAEMRKFLEKAGAYTLTPMSLMLRLATRVPGLSDPPSMRKV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCCHHHHHH
YRLGQGTPDRVTPARTRVIEALVEYGGLAFTLKELSELAGVTSTVVKGLAKQGVVEEADT
HHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHCCCCCCCCC
PRDAPYLTLDPGLPGKELTEDQEIAADRLQEGSRARRYGAVLLKGVTGSGKTEVYLEAVA
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHCEEEEEECCCCCCHHHHHHHHH
ECLRQGRQALVLLPEIALTSEFLTRVKARFGADPAEWHSGVTMTERRRCWRMVGQGEAQL
HHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCEEE
VVGARSALFLPYRDLGLIVVDEEHDTSYKQDEGASYNARDMAVLRASLNEAQVVLASATP
EEECCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
SLETWSNAQAGKYTRLDLVSRFGPQDLPDMASIDMRLEDLPGGSWLSPSLTSAVGARLQA
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCEEECCCCCCCCCCHHHHHHHHHHHC
GEQALLFLNRRGYAPVTLCRACGEMVGCDHCDARMVEHRFQKRLICHQCGETKPLPEVCP
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
SCGAEGKMAAVGPGVERIAEEAAAAFPDAKLAILSSDLFGSARALKAQIEELANGDTDLI
CCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEE
IGTQLVAKGHNFPRLTLVGVVDADLGLQGSDLRAAERTFQLMRQVAGRAGRADKPGQALL
EEHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QTFQPEHPVIRAILSGDEEGFWRTEAAEREAAGMPPYGRLAGIIISSTEAAAAFDLGTHL
HHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHEEEEEECCCHHHHHHHHHHH
ARNDGPIREAGGIVYGPAPAPIARVRGRHRVRLLVKAPKGVALQPALSRWTHGLKLPGHL
HCCCCCHHHCCCEEECCCCCCHHHHCCCCEEEEEEECCCCCEECHHHHHHHCCCCCCCEE
RLAIDIDPQNFF
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]