Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 89053469

GI number: 89053469

Start: 933550

End: 934818

Strand: Direct

Name: tilS [H]

Synonym: Jann_0978

Alternate gene names: 89053469

Gene position: 933550-934818 (Clockwise)

Preceding gene: 89053468

Following gene: 89053470

Centisome position: 21.62

GC content: 63.2

Gene sequence:

>1269_bases
ATGACGCGCGATCTGGTCCAGCGTTTCGCTGACCAAATGGGTCGGTTGTTGGGCCCGAACTTCCCATCAGACATCGCGCT
GGCCGTCAGCGGTGGCGGCGACAGCATGGCGATGCTGACCCTGGCCCATGGGTGGGCGCGGGTCTATGGCGTTGGCCTTC
ACGTCGTCACAGTCGATCACGGTCTGCGCAGTGCAAGCCGCCAGGAGGCCGAGATGGTGGCGAAAGAATGTGCAGTCTTG
GGACACCCCCACACGACCCTAAAGTGGCAGTGGGACAATCAGGGAAATCTACAAGACGCGGCGCGGCGCGCGCGGCTGTC
GTTGATTGGCGCATGGCGCGGTCAGATCGCGCACGTTCTGTTTGCCCATACACGAGACGATCAGGCCGAAACGATGTTGA
TGCGGCTCTTGCGCGGATCGGGGGTAGACGGCCTGTCGGCTATGGCTGAGACCCGCGATATGGACGGGTGGCAGGTCATC
CGCCCCCTTCTGTCAGAGACCCGCGCGGATCTGCGTCACTATGCCGACGTGCTGCGTGTGCCATACGTCGATGATCCCTC
CAACGAAGACGAAACCTTCGACCGTGTGCGTTTGCGCGGCACGATCAAGGCGTTGGGTATGGACACCAGAGCCTTGGCCA
ACACGGCCGCACGCATGGGCCGCGCGCGCACAGCGCTTGAGGCGCGGGCTGTGGACGTGGCACGCGCCTGCGTGACGGAG
GACCGTTGGGAATGGATGCCCACCGGTGACTTGCTGATTGACCGTGACCGCTTCGCTGAGGTCGAACCGGATACGCAATT
GCGCGTGTTGGCTGGCGCATTGCAGTGGGTTACGACGGCTGACTACCGTCCCCGGGCCGCGCCTCTGGAAGATCTGTTGG
ATCGCGCCCTGTCCGGCGGCGGCGGGACGCTTCACGGGGCGCGAACAACGATCCGCGGCGCCCATATTCGTGTCAGTCGG
GAATTTGATGCGATTGAGGGAACGGAGGTGGCATTGGGATCTGAGGCCGTGATCTGGGACACGCGCTGGCAGGTTTTTTC
GACGGACGTCCCAAGCGGCCGGAGGGTGCGAGCCCTCGGCCCGGACGGTTGGCAGCAGGCAACCGACAAGCCAGATCACC
CGCCGCCCCATGATGCGGCAATCTGTTTGCCTGCATTGTTTGAGGGCGATCGTTTGGTCGCATGGCATCCGGCACACGGT
TCCCCAGGATTTCAGGTCAAATTCAAGCCGCCACGTGGGCATTTCACCGCATTCTTGGTTTCGCGTTGA

Upstream 100 bases:

>100_bases
CTGACCCTGTCAGAGGTTGGCGTCCGCTATCCGGGCAGTGCCTCGGTCGCGGACGCGCAGGCGGAAATGGGGCGGTTGGG
CTGCCAGTGAGCGCAGGCCT

Downstream 100 bases:

>100_bases
ACTCAAGGCTGCGACGCTTATCTTAGTATCAACGCGCGGGTCGGACCTGCGCCCTTCCCGTTTGAGGAGACGTGCCTTGG
GCAACGCGAGAAATATCGCA

Product: tRNA(Ile)-lysidine synthetase-like protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTRDLVQRFADQMGRLLGPNFPSDIALAVSGGGDSMAMLTLAHGWARVYGVGLHVVTVDHGLRSASRQEAEMVAKECAVL
GHPHTTLKWQWDNQGNLQDAARRARLSLIGAWRGQIAHVLFAHTRDDQAETMLMRLLRGSGVDGLSAMAETRDMDGWQVI
RPLLSETRADLRHYADVLRVPYVDDPSNEDETFDRVRLRGTIKALGMDTRALANTAARMGRARTALEARAVDVARACVTE
DRWEWMPTGDLLIDRDRFAEVEPDTQLRVLAGALQWVTTADYRPRAAPLEDLLDRALSGGGGTLHGARTTIRGAHIRVSR
EFDAIEGTEVALGSEAVIWDTRWQVFSTDVPSGRRVRALGPDGWQQATDKPDHPPPHDAAICLPALFEGDRLVAWHPAHG
SPGFQVKFKPPRGHFTAFLVSR

Sequences:

>Translated_422_residues
MTRDLVQRFADQMGRLLGPNFPSDIALAVSGGGDSMAMLTLAHGWARVYGVGLHVVTVDHGLRSASRQEAEMVAKECAVL
GHPHTTLKWQWDNQGNLQDAARRARLSLIGAWRGQIAHVLFAHTRDDQAETMLMRLLRGSGVDGLSAMAETRDMDGWQVI
RPLLSETRADLRHYADVLRVPYVDDPSNEDETFDRVRLRGTIKALGMDTRALANTAARMGRARTALEARAVDVARACVTE
DRWEWMPTGDLLIDRDRFAEVEPDTQLRVLAGALQWVTTADYRPRAAPLEDLLDRALSGGGGTLHGARTTIRGAHIRVSR
EFDAIEGTEVALGSEAVIWDTRWQVFSTDVPSGRRVRALGPDGWQQATDKPDHPPPHDAAICLPALFEGDRLVAWHPAHG
SPGFQVKFKPPRGHFTAFLVSR
>Mature_421_residues
TRDLVQRFADQMGRLLGPNFPSDIALAVSGGGDSMAMLTLAHGWARVYGVGLHVVTVDHGLRSASRQEAEMVAKECAVLG
HPHTTLKWQWDNQGNLQDAARRARLSLIGAWRGQIAHVLFAHTRDDQAETMLMRLLRGSGVDGLSAMAETRDMDGWQVIR
PLLSETRADLRHYADVLRVPYVDDPSNEDETFDRVRLRGTIKALGMDTRALANTAARMGRARTALEARAVDVARACVTED
RWEWMPTGDLLIDRDRFAEVEPDTQLRVLAGALQWVTTADYRPRAAPLEDLLDRALSGGGGTLHGARTTIRGAHIRVSRE
FDAIEGTEVALGSEAVIWDTRWQVFSTDVPSGRRVRALGPDGWQQATDKPDHPPPHDAAICLPALFEGDRLVAWHPAHGS
PGFQVKFKPPRGHFTAFLVSR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=178, Percent_Identity=34.2696629213483, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 46626; Mature: 46495

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDLVQRFADQMGRLLGPNFPSDIALAVSGGGDSMAMLTLAHGWARVYGVGLHVVTVDH
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHEEEEEEEEEECC
GLRSASRQEAEMVAKECAVLGHPHTTLKWQWDNQGNLQDAARRARLSLIGAWRGQIAHVL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FAHTRDDQAETMLMRLLRGSGVDGLSAMAETRDMDGWQVIRPLLSETRADLRHYADVLRV
EEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PYVDDPSNEDETFDRVRLRGTIKALGMDTRALANTAARMGRARTALEARAVDVARACVTE
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DRWEWMPTGDLLIDRDRFAEVEPDTQLRVLAGALQWVTTADYRPRAAPLEDLLDRALSGG
CCCCCCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
GGTLHGARTTIRGAHIRVSREFDAIEGTEVALGSEAVIWDTRWQVFSTDVPSGRRVRALG
CCEECCCCEEEEEEEEEEECCCCCCCCCEEEECCCEEEEECCEEEEECCCCCCCEEEEEC
PDGWQQATDKPDHPPPHDAAICLPALFEGDRLVAWHPAHGSPGFQVKFKPPRGHFTAFLV
CCCHHHCCCCCCCCCCCCHHEEEHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEEEEEE
SR
EC
>Mature Secondary Structure 
TRDLVQRFADQMGRLLGPNFPSDIALAVSGGGDSMAMLTLAHGWARVYGVGLHVVTVDH
CHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHEEEEEEEEEECC
GLRSASRQEAEMVAKECAVLGHPHTTLKWQWDNQGNLQDAARRARLSLIGAWRGQIAHVL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FAHTRDDQAETMLMRLLRGSGVDGLSAMAETRDMDGWQVIRPLLSETRADLRHYADVLRV
EEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PYVDDPSNEDETFDRVRLRGTIKALGMDTRALANTAARMGRARTALEARAVDVARACVTE
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DRWEWMPTGDLLIDRDRFAEVEPDTQLRVLAGALQWVTTADYRPRAAPLEDLLDRALSGG
CCCCCCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
GGTLHGARTTIRGAHIRVSREFDAIEGTEVALGSEAVIWDTRWQVFSTDVPSGRRVRALG
CCEECCCCEEEEEEEEEEECCCCCCCCCEEEECCCEEEEECCEEEEECCCCCCCEEEEEC
PDGWQQATDKPDHPPPHDAAICLPALFEGDRLVAWHPAHGSPGFQVKFKPPRGHFTAFLV
CCCHHHCCCCCCCCCCCCHHEEEHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEEEEEE
SR
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA