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The map label for this gene is sucB [H]

Identifier: 89053323

GI number: 89053323

Start: 788078

End: 789601

Strand: Direct

Name: sucB [H]

Synonym: Jann_0832

Alternate gene names: 89053323

Gene position: 788078-789601 (Clockwise)

Preceding gene: 89053322

Following gene: 89053324

Centisome position: 18.25

GC content: 64.5

Gene sequence:

>1524_bases
ATGTCTGTAGAAGTTCGAGTGCCCACCCTGGGTGAATCCGTGACCGAGGCGACCGTGGCGACGTGGTTCAAGAAGCCTGG
CGATGCCGTGGCCGTCGACGAGATGCTGTGCGAGTTGGAGACTGACAAGGTGACGGTGGAAGTGCCGTCGCCCGCCGCAG
GCACCTTGGGCGAGATCGTCGCGGCAGAGGGCGAGACGGTTGGCGTGGATGCGTTGCTGGCCACGCTGAGCGAGGGCGAT
GCGGGGTCGGACGCGGCCCCCAAGGCCAAGGACGCTGCGGATGAGGGGACGTCCGGTGCGCCGCGCGAAGAGGCGAGCGG
CGATGCGGTGGACGTCATGGTGCCGACATTGGGCGAGTCCGTGACCGAAGCGACGGTGTCGACCTGGTTCAAGAAGGTGG
GCGATACCGTCGCGCAGGATGAGATGTTGTGTGAGTTGGAGACGGACAAGGTCTCCGTCGAAGTGCCCGCACCTGCGGCG
GGGGTGTTGAGCGAGATCCTGGCAGAGGAAGGGTCCACCGTGGAGGCGTCGGCCAAGTTGGCGGTGATCGGGGGGGCTGT
CGCCTCCGGCTCCGATGGTGGCTCCTCGGGAGCTGCCACTCCCTCCTCGCAGGGGTCCGGGGACAAGGATGTGTCCAACG
CGCCGTCGGCGGAGAAGTTGATGGCGGACAAGGGGCTGTCGGCGGATCAGGTGACCGGGACGGGTCGTGACGGGCGGATC
ATGAAGGAAGATGTGATGAAGGCGGCAGCGGCCCCGGCGCCTGCGGCGACCGCGCCCGCCCCGCCTGCCCAAACGCCCCG
CGCACCAGTGGCCGCGAATGACGAGGCGCGGGAGGAGCGGGTGAAGATGACCCGCCTGCGCCAGACCATCGCGCGACGGC
TGAAGGATGCCCAGAACAACGCGGCGATCCTGACGACGTATAATGAGGTCGACATGACCGAGGTCATGGCCTTGCGGACC
GAATACAAGGACCTGTTCCTGAAGAAGCATGGCGTGAAGCTCGGCTTCATGTCTTTCTTCACCAAGGCCTGCGTGCATGC
CCTGCGCGAGGTGCCGGAAGTCAACGCGGAGATCGATGGCACGGATATCGTCTACAAGAACTTCGTCCACATGGGCATTG
CAGCAGGAACACCCCAGGGCCTTGTGGTGCCGGTCATCCGTGACGTGGATCAGATGGGCTTTGCCGATATCGAGAAGGCG
ATTGGTGAGAAGGGCGCGAAGGCCCGCGACGGCAAGCTGTCGATGGCCGAGATGCAGGGGGGCACGTTCACGATCTCCAA
CGGCGGGGTCTATGGCTCGCTGATGTCCTCGCCCATTCTGAACCCGCCGCAATCGGGCATTCTGGGGATGCACAAGATCC
AGGACCGTCCGATGGCAATCGGCGGAGAGGTCGTGATCCGCCCGATGATGTATCTGGCCCTGTCTTATGATCACCGGATC
GTGGACGGCAAAGGGGCGGTGACGTTTCTGGTCCGCGTGAAAGAGGCGTTGGAGGATCCCCGACGTTTGCTGATGGACCT
GTAA

Upstream 100 bases:

>100_bases
CACAGCCTCGGCCTCGCCCGCGACGGGCCTGGCCTCCGCCCACAAATCCCAACAAGCCGCACTCGTTGACAGTGCACTGA
CCATCGAAGGGTAAGAGAAA

Downstream 100 bases:

>100_bases
TTGGGCGCCGCCGCCATCGCCTACGCAGAGGCGATTGCGCGGCGGCGGGCGTTCCGGCTTCCGGGCTATACGACGCTGGC
GGAATGCGGGTTTGATGGGG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSVEVRVPTLGESVTEATVATWFKKPGDAVAVDEMLCELETDKVTVEVPSPAAGTLGEIVAAEGETVGVDALLATLSEGD
AGSDAAPKAKDAADEGTSGAPREEASGDAVDVMVPTLGESVTEATVSTWFKKVGDTVAQDEMLCELETDKVSVEVPAPAA
GVLSEILAEEGSTVEASAKLAVIGGAVASGSDGGSSGAATPSSQGSGDKDVSNAPSAEKLMADKGLSADQVTGTGRDGRI
MKEDVMKAAAAPAPAATAPAPPAQTPRAPVAANDEAREERVKMTRLRQTIARRLKDAQNNAAILTTYNEVDMTEVMALRT
EYKDLFLKKHGVKLGFMSFFTKACVHALREVPEVNAEIDGTDIVYKNFVHMGIAAGTPQGLVVPVIRDVDQMGFADIEKA
IGEKGAKARDGKLSMAEMQGGTFTISNGGVYGSLMSSPILNPPQSGILGMHKIQDRPMAIGGEVVIRPMMYLALSYDHRI
VDGKGAVTFLVRVKEALEDPRRLLMDL

Sequences:

>Translated_507_residues
MSVEVRVPTLGESVTEATVATWFKKPGDAVAVDEMLCELETDKVTVEVPSPAAGTLGEIVAAEGETVGVDALLATLSEGD
AGSDAAPKAKDAADEGTSGAPREEASGDAVDVMVPTLGESVTEATVSTWFKKVGDTVAQDEMLCELETDKVSVEVPAPAA
GVLSEILAEEGSTVEASAKLAVIGGAVASGSDGGSSGAATPSSQGSGDKDVSNAPSAEKLMADKGLSADQVTGTGRDGRI
MKEDVMKAAAAPAPAATAPAPPAQTPRAPVAANDEAREERVKMTRLRQTIARRLKDAQNNAAILTTYNEVDMTEVMALRT
EYKDLFLKKHGVKLGFMSFFTKACVHALREVPEVNAEIDGTDIVYKNFVHMGIAAGTPQGLVVPVIRDVDQMGFADIEKA
IGEKGAKARDGKLSMAEMQGGTFTISNGGVYGSLMSSPILNPPQSGILGMHKIQDRPMAIGGEVVIRPMMYLALSYDHRI
VDGKGAVTFLVRVKEALEDPRRLLMDL
>Mature_506_residues
SVEVRVPTLGESVTEATVATWFKKPGDAVAVDEMLCELETDKVTVEVPSPAAGTLGEIVAAEGETVGVDALLATLSEGDA
GSDAAPKAKDAADEGTSGAPREEASGDAVDVMVPTLGESVTEATVSTWFKKVGDTVAQDEMLCELETDKVSVEVPAPAAG
VLSEILAEEGSTVEASAKLAVIGGAVASGSDGGSSGAATPSSQGSGDKDVSNAPSAEKLMADKGLSADQVTGTGRDGRIM
KEDVMKAAAAPAPAATAPAPPAQTPRAPVAANDEAREERVKMTRLRQTIARRLKDAQNNAAILTTYNEVDMTEVMALRTE
YKDLFLKKHGVKLGFMSFFTKACVHALREVPEVNAEIDGTDIVYKNFVHMGIAAGTPQGLVVPVIRDVDQMGFADIEKAI
GEKGAKARDGKLSMAEMQGGTFTISNGGVYGSLMSSPILNPPQSGILGMHKIQDRPMAIGGEVVIRPMMYLALSYDHRIV
DGKGAVTFLVRVKEALEDPRRLLMDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=250, Percent_Identity=58.4, Blast_Score=308, Evalue=7e-84,
Organism=Homo sapiens, GI31711992, Length=560, Percent_Identity=28.75, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=28.5046728971963, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI203098816, Length=485, Percent_Identity=28.8659793814433, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=28.2608695652174, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.1614906832298, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786946, Length=406, Percent_Identity=50.9852216748768, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1786305, Length=533, Percent_Identity=32.4577861163227, Blast_Score=220, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI25146366, Length=410, Percent_Identity=44.1463414634146, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=31.9727891156463, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=435, Percent_Identity=28.2758620689655, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=30.9446254071661, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=413, Percent_Identity=42.3728813559322, Blast_Score=328, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=25.8849557522124, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24645909, Length=245, Percent_Identity=56.734693877551, Blast_Score=291, Evalue=8e-79,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=29.6912114014252, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 52916; Mature: 52784

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEVRVPTLGESVTEATVATWFKKPGDAVAVDEMLCELETDKVTVEVPSPAAGTLGEIV
CCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
AAEGETVGVDALLATLSEGDAGSDAAPKAKDAADEGTSGAPREEASGDAVDVMVPTLGES
HCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
VTEATVSTWFKKVGDTVAQDEMLCELETDKVSVEVPAPAAGVLSEILAEEGSTVEASAKL
HHHHHHHHHHHHHCCHHHHCHHEEEEECCEEEEECCCCHHHHHHHHHHCCCCEEECCCEE
AVIGGAVASGSDGGSSGAATPSSQGSGDKDVSNAPSAEKLMADKGLSADQVTGTGRDGRI
EEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCH
MKEDVMKAAAAPAPAATAPAPPAQTPRAPVAANDEAREERVKMTRLRQTIARRLKDAQNN
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AAILTTYNEVDMTEVMALRTEYKDLFLKKHGVKLGFMSFFTKACVHALREVPEVNAEIDG
EEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC
TDIVYKNFVHMGIAAGTPQGLVVPVIRDVDQMGFADIEKAIGEKGAKARDGKLSMAEMQG
CHHHHHHHHHHHEECCCCCCEEEHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEHEEECC
GTFTISNGGVYGSLMSSPILNPPQSGILGMHKIQDRPMAIGGEVVIRPMMYLALSYDHRI
CEEEEECCCEEHHHHCCCCCCCCCCCCCCHHHHCCCCCEECCHHHHHHHHHHHHCCCCEE
VDGKGAVTFLVRVKEALEDPRRLLMDL
ECCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVEVRVPTLGESVTEATVATWFKKPGDAVAVDEMLCELETDKVTVEVPSPAAGTLGEIV
CEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
AAEGETVGVDALLATLSEGDAGSDAAPKAKDAADEGTSGAPREEASGDAVDVMVPTLGES
HCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
VTEATVSTWFKKVGDTVAQDEMLCELETDKVSVEVPAPAAGVLSEILAEEGSTVEASAKL
HHHHHHHHHHHHHCCHHHHCHHEEEEECCEEEEECCCCHHHHHHHHHHCCCCEEECCCEE
AVIGGAVASGSDGGSSGAATPSSQGSGDKDVSNAPSAEKLMADKGLSADQVTGTGRDGRI
EEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCH
MKEDVMKAAAAPAPAATAPAPPAQTPRAPVAANDEAREERVKMTRLRQTIARRLKDAQNN
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AAILTTYNEVDMTEVMALRTEYKDLFLKKHGVKLGFMSFFTKACVHALREVPEVNAEIDG
EEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC
TDIVYKNFVHMGIAAGTPQGLVVPVIRDVDQMGFADIEKAIGEKGAKARDGKLSMAEMQG
CHHHHHHHHHHHEECCCCCCEEEHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEHEEECC
GTFTISNGGVYGSLMSSPILNPPQSGILGMHKIQDRPMAIGGEVVIRPMMYLALSYDHRI
CEEEEECCCEEHHHHCCCCCCCCCCCCCCHHHHCCCCCEECCHHHHHHHHHHHHCCCCEE
VDGKGAVTFLVRVKEALEDPRRLLMDL
ECCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]