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The map label for this gene is miaB

Identifier: 89053188

GI number: 89053188

Start: 669900

End: 671288

Strand: Direct

Name: miaB

Synonym: Jann_0697

Alternate gene names: 89053188

Gene position: 669900-671288 (Clockwise)

Preceding gene: 89053186

Following gene: 89053189

Centisome position: 15.51

GC content: 62.49

Gene sequence:

>1389_bases
TTGATTGAAGTAAGCGCGGTCGCAGGCTACACCCCATCCGTTGAGATTTCCCTGACCCCGCGCGAGGCGCCCCAGATGAC
CAAAAAGCTCTTTGTGAAGACCTATGGCTGTCAGATGAACGTCTACGACAGTGAGCGTATGGCCGAGGCCTTGGGCGGCG
AGGGATACGAGCAGGTGAACACGCCCGAAGGGGCGGATATGATCCTGCTCAACACCTGCCATATCCGTGAAAAGGCTGCG
GAAAAGATGTATTCTGAACTGGGGCGGTTGCGGCCCTTGCGCGATGCCAACCCGGATTTGAAGATCGGCGTGGCGGGCTG
TGTGGCGCAGGCGGAGGGTGAGGAAATCATGCGCCGTCAGCCGCTTGTGGACCTTGTTGTGGGGCCTCAGACGTACCACC
GCCTGCCGAAGATGATGGAGGCGGTGAACGCCGGTGAAAAGGCGCTCGACACGGATTTCCCTGAGGAAGACAAGTTCCTG
AACCTGCCCAAGGCGCGCGCCACGCGCGGCCCCACCGCGTTCCTGACGGTGCAGGAGGGCTGCGATAAATTCTGCGCCTT
CTGTGTGGTGCCCTATACGCGCGGCGCGGAGGTCTCCCGGTCCGCTGAGCGGTTGATGGCCGAAGCCCGCGATCTGGTGG
ACCGGGGTGTCCGAGAGATCACGCTGCTGGGGCAGAATGTGAATGCCTATCACGGGGCGGGGGATGGCGGGACCTGGGGG
CTTGCACGCCTGATCCGCGAGATGGCGAAGATCGACGGGCTAGACCGTATCCGCTTCACGACCTCCCACCCCAACGATAT
GGAAGACGACCTGATCGCGGCCCATGGCGATTGCCCGGAACTGATGCCGTATCTCCACCTTCCCGTGCAGGCCGGATCAG
ACAAAATCCTCAAGGCGATGAACCGCAAGCACACGGCGGCGGACTACATTCGTCTGATCGACCGCATTCGGGATGCCCGC
CCGGATCTGCACCTGTCGGGTGATTTCATCGTCGGCTTCCCCGGCGAGACGGAGGAGGATTTTCAGGCGACCCTCGATCT
GGTGGAAACGGTCGGCTACGGCACGGCCTATTCGTTCAAATACTCCGCCCGGCCCGGGACCCCTGCGGCGGAACGGACGG
GTCAGGTGAGCGAGGCCGAGGCCAGTGACCGTTTGCAGCGCCTGCAAGCGCTGCTGACCCAACAGCAACGCGCGGCGCAA
GATGCAATGGTCGGGCGGCGCGTGAAGGTGCTGTTTGAGAAGCCGGGCCGCAACCCGGGACAGATGATCGGGAAGTCCGA
GTACCTGCATTCGGTGCACGTGGATGGGCCGGACACTCTGCGCGGTCAGATTGCCGAGGTCGAGATCGCTGAAAGTAAAA
CGAACTCTCTCACCGGTCGGCTGGTGTAA

Upstream 100 bases:

>100_bases
CTTGAAGCTGGACAGGGTCTCAAAATTCATCGGGTCGTCTGCTCGGGTATCCGTTTTGCGATACGATGCCTTTGTGGGCG
TGGTTTTGCAATTGGCGGGA

Downstream 100 bases:

>100_bases
GGCGACGTCTCGGGCGGGCGGTGGCCTGCCTTGCGGGCCGAGCGATCCGGCGACAGATCAGGGTGGATCGGCGTAATGTC
GCTGATTTGGCAACAAAAAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MIEVSAVAGYTPSVEISLTPREAPQMTKKLFVKTYGCQMNVYDSERMAEALGGEGYEQVNTPEGADMILLNTCHIREKAA
EKMYSELGRLRPLRDANPDLKIGVAGCVAQAEGEEIMRRQPLVDLVVGPQTYHRLPKMMEAVNAGEKALDTDFPEEDKFL
NLPKARATRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRSAERLMAEARDLVDRGVREITLLGQNVNAYHGAGDGGTWG
LARLIREMAKIDGLDRIRFTTSHPNDMEDDLIAAHGDCPELMPYLHLPVQAGSDKILKAMNRKHTAADYIRLIDRIRDAR
PDLHLSGDFIVGFPGETEEDFQATLDLVETVGYGTAYSFKYSARPGTPAAERTGQVSEAEASDRLQRLQALLTQQQRAAQ
DAMVGRRVKVLFEKPGRNPGQMIGKSEYLHSVHVDGPDTLRGQIAEVEIAESKTNSLTGRLV

Sequences:

>Translated_462_residues
MIEVSAVAGYTPSVEISLTPREAPQMTKKLFVKTYGCQMNVYDSERMAEALGGEGYEQVNTPEGADMILLNTCHIREKAA
EKMYSELGRLRPLRDANPDLKIGVAGCVAQAEGEEIMRRQPLVDLVVGPQTYHRLPKMMEAVNAGEKALDTDFPEEDKFL
NLPKARATRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRSAERLMAEARDLVDRGVREITLLGQNVNAYHGAGDGGTWG
LARLIREMAKIDGLDRIRFTTSHPNDMEDDLIAAHGDCPELMPYLHLPVQAGSDKILKAMNRKHTAADYIRLIDRIRDAR
PDLHLSGDFIVGFPGETEEDFQATLDLVETVGYGTAYSFKYSARPGTPAAERTGQVSEAEASDRLQRLQALLTQQQRAAQ
DAMVGRRVKVLFEKPGRNPGQMIGKSEYLHSVHVDGPDTLRGQIAEVEIAESKTNSLTGRLV
>Mature_462_residues
MIEVSAVAGYTPSVEISLTPREAPQMTKKLFVKTYGCQMNVYDSERMAEALGGEGYEQVNTPEGADMILLNTCHIREKAA
EKMYSELGRLRPLRDANPDLKIGVAGCVAQAEGEEIMRRQPLVDLVVGPQTYHRLPKMMEAVNAGEKALDTDFPEEDKFL
NLPKARATRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRSAERLMAEARDLVDRGVREITLLGQNVNAYHGAGDGGTWG
LARLIREMAKIDGLDRIRFTTSHPNDMEDDLIAAHGDCPELMPYLHLPVQAGSDKILKAMNRKHTAADYIRLIDRIRDAR
PDLHLSGDFIVGFPGETEEDFQATLDLVETVGYGTAYSFKYSARPGTPAAERTGQVSEAEASDRLQRLQALLTQQQRAAQ
DAMVGRRVKVLFEKPGRNPGQMIGKSEYLHSVHVDGPDTLRGQIAEVEIAESKTNSLTGRLV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=432, Percent_Identity=33.3333333333333, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI28872782, Length=414, Percent_Identity=33.8164251207729, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI93277076, Length=450, Percent_Identity=28, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=50.1133786848073, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=30.5309734513274, Blast_Score=185, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17553146, Length=484, Percent_Identity=31.404958677686, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=368, Percent_Identity=26.9021739130435, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=32.6315789473684, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI19922432, Length=470, Percent_Identity=26.8085106382979, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_JANSC (Q28UJ8)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508639.1
- ProteinModelPortal:   Q28UJ8
- STRING:   Q28UJ8
- GeneID:   3933140
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0697
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- PhylomeDB:   Q28UJ8
- ProtClustDB:   PRK14325
- BioCyc:   JSP290400:JANN_0697-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50967; Mature: 50967

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVSAVAGYTPSVEISLTPREAPQMTKKLFVKTYGCQMNVYDSERMAEALGGEGYEQVN
CEEECEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCCCCC
TPEGADMILLNTCHIREKAAEKMYSELGRLRPLRDANPDLKIGVAGCVAQAEGEEIMRRQ
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHCCHHHHHHHC
PLVDLVVGPQTYHRLPKMMEAVNAGEKALDTDFPEEDKFLNLPKARATRGPTAFLTVQEG
CCCEEEECCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEECCCHHHCCCCCEEEEEHHHH
CDKFCAFCVVPYTRGAEVSRSAERLMAEARDLVDRGVREITLLGQNVNAYHGAGDGGTWG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHH
LARLIREMAKIDGLDRIRFTTSHPNDMEDDLIAAHGDCPELMPYLHLPVQAGSDKILKAM
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHCCCCCCCCHHHHHHH
NRKHTAADYIRLIDRIRDARPDLHLSGDFIVGFPGETEEDFQATLDLVETVGYGTAYSFK
HCCCHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEE
YSARPGTPAAERTGQVSEAEASDRLQRLQALLTQQQRAAQDAMVGRRVKVLFEKPGRNPG
ECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHH
QMIGKSEYLHSVHVDGPDTLRGQIAEVEIAESKTNSLTGRLV
HHCCCHHHHEEEECCCCHHHCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MIEVSAVAGYTPSVEISLTPREAPQMTKKLFVKTYGCQMNVYDSERMAEALGGEGYEQVN
CEEECEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCCCCC
TPEGADMILLNTCHIREKAAEKMYSELGRLRPLRDANPDLKIGVAGCVAQAEGEEIMRRQ
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHCCHHHHHHHC
PLVDLVVGPQTYHRLPKMMEAVNAGEKALDTDFPEEDKFLNLPKARATRGPTAFLTVQEG
CCCEEEECCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEECCCHHHCCCCCEEEEEHHHH
CDKFCAFCVVPYTRGAEVSRSAERLMAEARDLVDRGVREITLLGQNVNAYHGAGDGGTWG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHH
LARLIREMAKIDGLDRIRFTTSHPNDMEDDLIAAHGDCPELMPYLHLPVQAGSDKILKAM
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHCCCCCCCCHHHHHHH
NRKHTAADYIRLIDRIRDARPDLHLSGDFIVGFPGETEEDFQATLDLVETVGYGTAYSFK
HCCCHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEE
YSARPGTPAAERTGQVSEAEASDRLQRLQALLTQQQRAAQDAMVGRRVKVLFEKPGRNPG
ECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHH
QMIGKSEYLHSVHVDGPDTLRGQIAEVEIAESKTNSLTGRLV
HHCCCHHHHEEEECCCCHHHCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA