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The map label for this gene is lpdA-2 [H]

Identifier: 88658423

GI number: 88658423

Start: 1016418

End: 1017824

Strand: Direct

Name: lpdA-2 [H]

Synonym: ECH_0992

Alternate gene names: 88658423

Gene position: 1016418-1017824 (Clockwise)

Preceding gene: 88657799

Following gene: 88657676

Centisome position: 86.41

GC content: 32.27

Gene sequence:

>1407_bases
ATGACTAAACATGAATTTCTTATAATTGGAAGCGGTCCAGGAGGATATATAGCAGCTATAAGAGCTGCACAACTAGGATA
CGATGTTGCAATAATTGAAAAAGAAAATACATTAGGTGGAATCTGTTTAAATTGGGGATGTATCCCAACAAAATCACTAC
TACAATCTGCATCAGTTTACCATAACATCAAGAAAGCAGGTATTTTTGGGATTACAGTACAAGACATAAAATTTGATTTT
AATAAGATAATAGAGCGTTCACGTGATGTTGTTACAAAACTATCAAACGGCATTAGTGGATTAATGAAAAAAAATAACAT
AAAAGTTTACTATGGTACTGCAAAACTACTAGGTAATAGCACAGTAGAAATTACAGATCACTCAAACAAAATAATTAATG
TTACATCAACTCACATCATTCTTGCAACAGGATCTAAAGCCAGAAATATACCAGGTATAGATTTTGATAATAAAATAGTT
TGGAATGCGAAAAATGCTATGACACCAGATAAATTCCCTAAATCTTTATTGATCATAGGTAGCGGAGCCATAGGGATAGA
ATTTGCGAGCTTTTATAATACTTTTGGTACTGAAGTTACAATAGTAGAATTAAAGGACAATATACTTCCACTTGAAGATC
ATGAAATATCCGAATGTATGCATAATATTTTAAGTAATAAAGGAATCAAAATTCATACAAAAAGTTCAGTAACAAAATTA
GAAAAATTCAACAATTACGCTAAAATTCAGATCTCAGATACAATACATTTACAAGTAGATAAAATTATATTAGCAGCAGG
AGTACAACCCAACTCTAATGATATTGGTCTTGAAAACACACAAATTAAAACAGATGCTGCAGGATTCATTATTACTGACC
AGTACTGCTGTACAAATGAACTAGGAGTATATGCTATAGGAGATGTTGCTGGAGCTCCATGCTTAGCTCATAAAGCAAGC
CATGAAGCAGTTCTATGTGTAGAAAATATTGCTGAAAAAGAAAACAAAACAACAAATAAAACACATCCTATAAATAAAAA
TAATATACCTAGTTGCATATTCTCAATACCACAAATAGCTAGTGTTGGGTTAACTGAAAATCAAGCAAGAACTCAAGGTT
ATGATATAAAAGTTGGAAAATTTAATGCAAACTGCAATGGAAAAGCAGTAGCTATAGATGAAACGGAAGGTTTTGTTAAA
GTTATCATAGATAAATCTACTGGAGAGTTATTAGGTGCACATATGATAGGAGCAGAAGTTACAGAAATGATTCATGGATA
TGTCACCGGAAAACAAATAGAAGCTACTGATCAGGACATAATGTCATCTATCTTCCCTCATCCAACACTATCAGAAATGA
TACATGAGGCAGTACTATCATCAAACAACGGTTCACTTAACAGCTAG

Upstream 100 bases:

>100_bases
TCATTAATAGATACAACCTCGTAACCTTTCAAAATGTTAAACTTGCAAATATCTTAACTCCCTACTATCATTACTTACTC
TTATCTAAAAAAACAAAAGC

Downstream 100 bases:

>100_bases
TTTATGTTTTCTTTAATACTCGATTACCGTATTATTTATTACAAGTACGTCAAGCTAAGTTGTTTTACTTAATAAAAATA
CATATTTTTCCCTGCAATTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTKHEFLIIGSGPGGYIAAIRAAQLGYDVAIIEKENTLGGICLNWGCIPTKSLLQSASVYHNIKKAGIFGITVQDIKFDF
NKIIERSRDVVTKLSNGISGLMKKNNIKVYYGTAKLLGNSTVEITDHSNKIINVTSTHIILATGSKARNIPGIDFDNKIV
WNAKNAMTPDKFPKSLLIIGSGAIGIEFASFYNTFGTEVTIVELKDNILPLEDHEISECMHNILSNKGIKIHTKSSVTKL
EKFNNYAKIQISDTIHLQVDKIILAAGVQPNSNDIGLENTQIKTDAAGFIITDQYCCTNELGVYAIGDVAGAPCLAHKAS
HEAVLCVENIAEKENKTTNKTHPINKNNIPSCIFSIPQIASVGLTENQARTQGYDIKVGKFNANCNGKAVAIDETEGFVK
VIIDKSTGELLGAHMIGAEVTEMIHGYVTGKQIEATDQDIMSSIFPHPTLSEMIHEAVLSSNNGSLNS

Sequences:

>Translated_468_residues
MTKHEFLIIGSGPGGYIAAIRAAQLGYDVAIIEKENTLGGICLNWGCIPTKSLLQSASVYHNIKKAGIFGITVQDIKFDF
NKIIERSRDVVTKLSNGISGLMKKNNIKVYYGTAKLLGNSTVEITDHSNKIINVTSTHIILATGSKARNIPGIDFDNKIV
WNAKNAMTPDKFPKSLLIIGSGAIGIEFASFYNTFGTEVTIVELKDNILPLEDHEISECMHNILSNKGIKIHTKSSVTKL
EKFNNYAKIQISDTIHLQVDKIILAAGVQPNSNDIGLENTQIKTDAAGFIITDQYCCTNELGVYAIGDVAGAPCLAHKAS
HEAVLCVENIAEKENKTTNKTHPINKNNIPSCIFSIPQIASVGLTENQARTQGYDIKVGKFNANCNGKAVAIDETEGFVK
VIIDKSTGELLGAHMIGAEVTEMIHGYVTGKQIEATDQDIMSSIFPHPTLSEMIHEAVLSSNNGSLNS
>Mature_467_residues
TKHEFLIIGSGPGGYIAAIRAAQLGYDVAIIEKENTLGGICLNWGCIPTKSLLQSASVYHNIKKAGIFGITVQDIKFDFN
KIIERSRDVVTKLSNGISGLMKKNNIKVYYGTAKLLGNSTVEITDHSNKIINVTSTHIILATGSKARNIPGIDFDNKIVW
NAKNAMTPDKFPKSLLIIGSGAIGIEFASFYNTFGTEVTIVELKDNILPLEDHEISECMHNILSNKGIKIHTKSSVTKLE
KFNNYAKIQISDTIHLQVDKIILAAGVQPNSNDIGLENTQIKTDAAGFIITDQYCCTNELGVYAIGDVAGAPCLAHKASH
EAVLCVENIAEKENKTTNKTHPINKNNIPSCIFSIPQIASVGLTENQARTQGYDIKVGKFNANCNGKAVAIDETEGFVKV
IIDKSTGELLGAHMIGAEVTEMIHGYVTGKQIEATDQDIMSSIFPHPTLSEMIHEAVLSSNNGSLNS

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=35.3319057815846, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI50301238, Length=471, Percent_Identity=28.6624203821656, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI22035672, Length=472, Percent_Identity=24.1525423728814, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=21.1538461538462, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=21.1538461538462, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=21.1538461538462, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=21.1538461538462, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=21.1538461538462, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI291045266, Length=456, Percent_Identity=21.9298245614035, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI291045268, Length=284, Percent_Identity=23.5915492957746, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786307, Length=455, Percent_Identity=31.2087912087912, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=25.9179265658747, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=28.9130434782609, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789915, Length=438, Percent_Identity=28.7671232876712, Blast_Score=178, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=33.974358974359, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=27.4633123689727, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=447, Percent_Identity=26.3982102908277, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=447, Percent_Identity=26.3982102908277, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=481, Percent_Identity=21.4137214137214, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=35.0730688935282, Blast_Score=262, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6325240, Length=484, Percent_Identity=28.9256198347107, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=473, Percent_Identity=27.6955602536998, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=33.6886993603412, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=21.6494845360825, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=21.6494845360825, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640551, Length=485, Percent_Identity=21.6494845360825, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17737741, Length=490, Percent_Identity=20.8163265306122, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50674; Mature: 50542

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHEFLIIGSGPGGYIAAIRAAQLGYDVAIIEKENTLGGICLNWGCIPTKSLLQSASVY
CCCCEEEEEECCCCCEEEEEEHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
HNIKKAGIFGITVQDIKFDFNKIIERSRDVVTKLSNGISGLMKKNNIKVYYGTAKLLGNS
HHHHHCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEHEECCCC
TVEITDHSNKIINVTSTHIILATGSKARNIPGIDFDNKIVWNAKNAMTPDKFPKSLLIIG
EEEEEECCCEEEEEEEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEE
SGAIGIEFASFYNTFGTEVTIVELKDNILPLEDHEISECMHNILSNKGIKIHTKSSVTKL
CCCCEEEHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHH
EKFNNYAKIQISDTIHLQVDKIILAAGVQPNSNDIGLENTQIKTDAAGFIITDQYCCTNE
HHCCCEEEEEECCEEEEEEEEEEEEECCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCC
LGVYAIGDVAGAPCLAHKASHEAVLCVENIAEKENKTTNKTHPINKNNIPSCIFSIPQIA
CCEEEECCCCCCCHHHHCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHH
SVGLTENQARTQGYDIKVGKFNANCNGKAVAIDETEGFVKVIIDKSTGELLGAHMIGAEV
HCCCCCCCHHCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHH
TEMIHGYVTGKQIEATDQDIMSSIFPHPTLSEMIHEAVLSSNNGSLNS
HHHHHHHCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TKHEFLIIGSGPGGYIAAIRAAQLGYDVAIIEKENTLGGICLNWGCIPTKSLLQSASVY
CCCEEEEEECCCCCEEEEEEHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
HNIKKAGIFGITVQDIKFDFNKIIERSRDVVTKLSNGISGLMKKNNIKVYYGTAKLLGNS
HHHHHCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEHEECCCC
TVEITDHSNKIINVTSTHIILATGSKARNIPGIDFDNKIVWNAKNAMTPDKFPKSLLIIG
EEEEEECCCEEEEEEEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEE
SGAIGIEFASFYNTFGTEVTIVELKDNILPLEDHEISECMHNILSNKGIKIHTKSSVTKL
CCCCEEEHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHH
EKFNNYAKIQISDTIHLQVDKIILAAGVQPNSNDIGLENTQIKTDAAGFIITDQYCCTNE
HHCCCEEEEEECCEEEEEEEEEEEEECCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCC
LGVYAIGDVAGAPCLAHKASHEAVLCVENIAEKENKTTNKTHPINKNNIPSCIFSIPQIA
CCEEEECCCCCCCHHHHCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHH
SVGLTENQARTQGYDIKVGKFNANCNGKAVAIDETEGFVKVIIDKSTGELLGAHMIGAEV
HCCCCCCCHHCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHH
TEMIHGYVTGKQIEATDQDIMSSIFPHPTLSEMIHEAVLSSNNGSLNS
HHHHHHHCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]