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The map label for this gene is pleC [H]

Identifier: 88658417

GI number: 88658417

Start: 909209

End: 910621

Strand: Reverse

Name: pleC [H]

Synonym: ECH_0885

Alternate gene names: 88658417

Gene position: 910621-909209 (Counterclockwise)

Preceding gene: 88658057

Following gene: 88658331

Centisome position: 77.42

GC content: 29.37

Gene sequence:

>1413_bases
ATGAAATATTTTCGAATTTTATTGTTAATGTTTTTAGTAATGACTTTGTGCTTTGCAGTTGGTTATACGCATATATATTT
GAGGGATGATTATCTGTATGAAGTTTCAAAAGAAATCAATGTCAATATTAAAGCAATACTTGTTAATTCTGTGATCAATA
AATATGCTCAATTATTAGCAAAGGCTCCAAGTCATGTATCAACAAATACGTACTATATGAATTTGTTGATAAAGTTACGT
GCTGAATTTCTCAAAGCTTTAGGTGGGGTTAAAGATTTTCATGTTATTTTATATAATGTAGATGGAAATGTAATATTTTC
TAATAGAAGTAGTGAAGATATTGCAGATCAGGACCTGCTGTTACGTTCTGAAGATATGAATTCATTATTAAGAGGAAAAT
ATGTAGACCATAGAGTAAGTAATGGAGCAACTACTGTGTCTATATTTCCTATATTTTCATTAGATGGTCAAGATAGTTCT
CCTTTAATGTTTTTAAAGATAATTAAAAATTCAGAAAATTTATCCAGTTTTATTGTAAATTTCTATACTGTGTTTTTAAT
GATAGTCATATTAGTCATTATTATATTTATTGTTGTAACTTGGTGTATATATTATAAAAATGCAAAAATGCTAAGTAAGC
AGTATAACGCAAATCTAAAATTAAAAGAAGCAAAGGAAGTAGCAGAACAAGAGAATGTAAGTAAATCACAATTTCTTGCA
AATGTTAGTCATGAGTTACGTACTCCATTGAACTCTATTATTGGGTTTTCGGAAATGATGAAGGCTGAGTCCATGGGGGC
TATAGAGAATGTTCATTATAAGGAGTATATAAATGATATTCACAATGCTGGTGTCCATCTATTAAGCTTAATTAATGATA
TTTTAGATTTCTCTAAGGCTGAGGCTGATAAATTAGTTGTAGAATTTGTAAAATTTGATTTAGGAAAAGTTATTTCTTCC
TGCTTTAATATGATACTTCCAAGAGCTGAGGAAGCAAAAGTAAGACTTGAACAAGTATTACCATCTAATCAAATTATAAT
GGTTGCAGATCCTAAAAGAATGAAACAAGTTATCATCAACTTATTATCCAATTCAATAAAATTTACTCCTGAAAATGGAT
GTGTAAAATTAGTGGTAGAATATGATCTCGAAAATAGCATGATTGTTATTGAAGTTATTGATAATGGTATAGGTATAGCA
CAGCAAGATTTGTATAAGGTTATGTCTGTTTTTGGGCAGGTAGATAGTAGACATGCTCGTAAATATGAAGGTACAGGGTT
AGGTTTACCTTTAAGTAAGAAATTAGTTGAACTGATGGATGGGGTATTTAAAATAAAGAGTGAACCTAATTTAGGTACTA
TAGTAACCTTAATGTTCCCGTATACTAATACCCTTGAAGAAAAAGGCTTCTAA

Upstream 100 bases:

>100_bases
GTGTGTAACTGTAACACGATATTGTTATATGTCAACTAAGAAAAAGTTTATAAATAGCACCATATACATATTATCATGCT
ATGGGTGATTGGGTATATAT

Downstream 100 bases:

>100_bases
TAGTTTGTGGTTCTATTTATAAAAGGGTGAATTATTTATTAGTATGCATGTGTTTTATAAGTCTATTTTTAATTGTAGTA
AGTAGCTTGAATTGATAAAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKYFRILLLMFLVMTLCFAVGYTHIYLRDDYLYEVSKEINVNIKAILVNSVINKYAQLLAKAPSHVSTNTYYMNLLIKLR
AEFLKALGGVKDFHVILYNVDGNVIFSNRSSEDIADQDLLLRSEDMNSLLRGKYVDHRVSNGATTVSIFPIFSLDGQDSS
PLMFLKIIKNSENLSSFIVNFYTVFLMIVILVIIIFIVVTWCIYYKNAKMLSKQYNANLKLKEAKEVAEQENVSKSQFLA
NVSHELRTPLNSIIGFSEMMKAESMGAIENVHYKEYINDIHNAGVHLLSLINDILDFSKAEADKLVVEFVKFDLGKVISS
CFNMILPRAEEAKVRLEQVLPSNQIIMVADPKRMKQVIINLLSNSIKFTPENGCVKLVVEYDLENSMIVIEVIDNGIGIA
QQDLYKVMSVFGQVDSRHARKYEGTGLGLPLSKKLVELMDGVFKIKSEPNLGTIVTLMFPYTNTLEEKGF

Sequences:

>Translated_470_residues
MKYFRILLLMFLVMTLCFAVGYTHIYLRDDYLYEVSKEINVNIKAILVNSVINKYAQLLAKAPSHVSTNTYYMNLLIKLR
AEFLKALGGVKDFHVILYNVDGNVIFSNRSSEDIADQDLLLRSEDMNSLLRGKYVDHRVSNGATTVSIFPIFSLDGQDSS
PLMFLKIIKNSENLSSFIVNFYTVFLMIVILVIIIFIVVTWCIYYKNAKMLSKQYNANLKLKEAKEVAEQENVSKSQFLA
NVSHELRTPLNSIIGFSEMMKAESMGAIENVHYKEYINDIHNAGVHLLSLINDILDFSKAEADKLVVEFVKFDLGKVISS
CFNMILPRAEEAKVRLEQVLPSNQIIMVADPKRMKQVIINLLSNSIKFTPENGCVKLVVEYDLENSMIVIEVIDNGIGIA
QQDLYKVMSVFGQVDSRHARKYEGTGLGLPLSKKLVELMDGVFKIKSEPNLGTIVTLMFPYTNTLEEKGF
>Mature_470_residues
MKYFRILLLMFLVMTLCFAVGYTHIYLRDDYLYEVSKEINVNIKAILVNSVINKYAQLLAKAPSHVSTNTYYMNLLIKLR
AEFLKALGGVKDFHVILYNVDGNVIFSNRSSEDIADQDLLLRSEDMNSLLRGKYVDHRVSNGATTVSIFPIFSLDGQDSS
PLMFLKIIKNSENLSSFIVNFYTVFLMIVILVIIIFIVVTWCIYYKNAKMLSKQYNANLKLKEAKEVAEQENVSKSQFLA
NVSHELRTPLNSIIGFSEMMKAESMGAIENVHYKEYINDIHNAGVHLLSLINDILDFSKAEADKLVVEFVKFDLGKVISS
CFNMILPRAEEAKVRLEQVLPSNQIIMVADPKRMKQVIINLLSNSIKFTPENGCVKLVVEYDLENSMIVIEVIDNGIGIA
QQDLYKVMSVFGQVDSRHARKYEGTGLGLPLSKKLVELMDGVFKIKSEPNLGTIVTLMFPYTNTLEEKGF

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=243, Percent_Identity=37.8600823045267, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=37.344398340249, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI48994928, Length=250, Percent_Identity=34.4, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1788713, Length=245, Percent_Identity=31.4285714285714, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081816, Length=243, Percent_Identity=32.5102880658436, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786783, Length=228, Percent_Identity=25.8771929824561, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788549, Length=237, Percent_Identity=24.4725738396624, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790861, Length=430, Percent_Identity=23.953488372093, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786600, Length=243, Percent_Identity=25.5144032921811, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790346, Length=394, Percent_Identity=22.3350253807107, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=22.8915662650602, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082128, Length=209, Percent_Identity=25.3588516746411, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1790436, Length=281, Percent_Identity=24.5551601423488, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788393, Length=243, Percent_Identity=23.4567901234568, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788279, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=160, Percent_Identity=36.875, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53308; Mature: 53308

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYFRILLLMFLVMTLCFAVGYTHIYLRDDYLYEVSKEINVNIKAILVNSVINKYAQLLA
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
KAPSHVSTNTYYMNLLIKLRAEFLKALGGVKDFHVILYNVDGNVIFSNRSSEDIADQDLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEECCCCCCCCCCHHHH
LRSEDMNSLLRGKYVDHRVSNGATTVSIFPIFSLDGQDSSPLMFLKIIKNSENLSSFIVN
HHHHHHHHHHHCCHHHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHH
FYTVFLMIVILVIIIFIVVTWCIYYKNAKMLSKQYNANLKLKEAKEVAEQENVSKSQFLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEHHHHHHHHHHCCCHHHHHH
NVSHELRTPLNSIIGFSEMMKAESMGAIENVHYKEYINDIHNAGVHLLSLINDILDFSKA
HHHHHHHCCHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EADKLVVEFVKFDLGKVISSCFNMILPRAEEAKVRLEQVLPSNQIIMVADPKRMKQVIIN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
LLSNSIKFTPENGCVKLVVEYDLENSMIVIEVIDNGIGIAQQDLYKVMSVFGQVDSRHAR
HHHCCCEECCCCCEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KYEGTGLGLPLSKKLVELMDGVFKIKSEPNLGTIVTLMFPYTNTLEEKGF
HCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCHHCCCC
>Mature Secondary Structure
MKYFRILLLMFLVMTLCFAVGYTHIYLRDDYLYEVSKEINVNIKAILVNSVINKYAQLLA
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
KAPSHVSTNTYYMNLLIKLRAEFLKALGGVKDFHVILYNVDGNVIFSNRSSEDIADQDLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEECCCCCCCCCCHHHH
LRSEDMNSLLRGKYVDHRVSNGATTVSIFPIFSLDGQDSSPLMFLKIIKNSENLSSFIVN
HHHHHHHHHHHCCHHHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHH
FYTVFLMIVILVIIIFIVVTWCIYYKNAKMLSKQYNANLKLKEAKEVAEQENVSKSQFLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEHHHHHHHHHHCCCHHHHHH
NVSHELRTPLNSIIGFSEMMKAESMGAIENVHYKEYINDIHNAGVHLLSLINDILDFSKA
HHHHHHHCCHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EADKLVVEFVKFDLGKVISSCFNMILPRAEEAKVRLEQVLPSNQIIMVADPKRMKQVIIN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
LLSNSIKFTPENGCVKLVVEYDLENSMIVIEVIDNGIGIAQQDLYKVMSVFGQVDSRHAR
HHHCCCEECCCCCEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KYEGTGLGLPLSKKLVELMDGVFKIKSEPNLGTIVTLMFPYTNTLEEKGF
HCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]