Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 88658370

GI number: 88658370

Start: 877676

End: 878308

Strand: Reverse

Name: nth [H]

Synonym: ECH_0857

Alternate gene names: 88658370

Gene position: 878308-877676 (Counterclockwise)

Preceding gene: 88657796

Following gene: 88657996

Centisome position: 74.67

GC content: 29.07

Gene sequence:

>633_bases
ATGAATCGAAGGAATATAAATTTACTCTTTACTAAATTTAAGGAGCATAATCCTCACCCCAGAATAGAACTGAAGTATAC
TAATAGTTTTACTTTACTAATAGCAATAGTTTTATCTGCACGTACTACTGATGTTAGTGTAAATAAGATTACAGATAAAC
TATTTGAAGTTGCTGATACTCCAAGAAAAATGTTAGATCTTGGAGAAAAAGGACTAAAAAATTATATAAATACAATAGGA
TTATATAATTCTAAATCTAAGAATATTATTGCACTTAGCGGAATTATTATAAATCAACACAATAGTAATGTACCTTTAGA
TTTTAATACTCTTGTTGCATTACCGGGTGTAGGTAGAAAAAGTGCTAATGTTTTTTTAAATACATGGTTAAATTTACCAA
CTGTTGCTGTTGATACTCATGTGTTTAGAGTAAGTAATAGGATAGGATTAGTTAAGGAAAGTAATGTATTAAAGACAGAG
GACGCGTTGTTAAATGTAATACCAAAAAAGTGGTTATTATATGCGCATCATTGGTTAGTATTACATGGTAGGTATGTGTG
TAAGTCAAGAAAGCCATTGTGTAGTCAGTGTGTTGTTCAAGATTTATGTGAATATGAAAGTAAAAGTTTATGA

Upstream 100 bases:

>100_bases
AGACTGAGAAACGTGAAAAAGTTGTTTCAGTAGCAAAGATAATTGATGCTAAGGATAATTCTGATATTGATGGTAATATA
TAAGTTGTGTATGCAGATTA

Downstream 100 bases:

>100_bases
ACTTGCTAGAGTGTGATGTTAAGTGTATTACTTGGTTAATATTGCATGATAGACATGTGTGTAAGTCAAGAAAGCCATTG
TGTAGTCAGTGTGTTGTTCA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNRRNINLLFTKFKEHNPHPRIELKYTNSFTLLIAIVLSARTTDVSVNKITDKLFEVADTPRKMLDLGEKGLKNYINTIG
LYNSKSKNIIALSGIIINQHNSNVPLDFNTLVALPGVGRKSANVFLNTWLNLPTVAVDTHVFRVSNRIGLVKESNVLKTE
DALLNVIPKKWLLYAHHWLVLHGRYVCKSRKPLCSQCVVQDLCEYESKSL

Sequences:

>Translated_210_residues
MNRRNINLLFTKFKEHNPHPRIELKYTNSFTLLIAIVLSARTTDVSVNKITDKLFEVADTPRKMLDLGEKGLKNYINTIG
LYNSKSKNIIALSGIIINQHNSNVPLDFNTLVALPGVGRKSANVFLNTWLNLPTVAVDTHVFRVSNRIGLVKESNVLKTE
DALLNVIPKKWLLYAHHWLVLHGRYVCKSRKPLCSQCVVQDLCEYESKSL
>Mature_210_residues
MNRRNINLLFTKFKEHNPHPRIELKYTNSFTLLIAIVLSARTTDVSVNKITDKLFEVADTPRKMLDLGEKGLKNYINTIG
LYNSKSKNIIALSGIIINQHNSNVPLDFNTLVALPGVGRKSANVFLNTWLNLPTVAVDTHVFRVSNRIGLVKESNVLKTE
DALLNVIPKKWLLYAHHWLVLHGRYVCKSRKPLCSQCVVQDLCEYESKSL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=29.9435028248588, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=54.3269230769231, Blast_Score=244, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=30.7262569832402, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=194, Percent_Identity=25.2577319587629, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550361, Length=183, Percent_Identity=30.6010928961749, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23866; Mature: 23866

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRNINLLFTKFKEHNPHPRIELKYTNSFTLLIAIVLSARTTDVSVNKITDKLFEVADT
CCCCCEEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
PRKMLDLGEKGLKNYINTIGLYNSKSKNIIALSGIIINQHNSNVPLDFNTLVALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHEEECCCCCCC
SANVFLNTWLNLPTVAVDTHVFRVSNRIGLVKESNVLKTEDALLNVIPKKWLLYAHHWLV
CCCEEEEECCCCCEEEEHHEEEEECCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHEEE
LHGRYVCKSRKPLCSQCVVQDLCEYESKSL
EECHHEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRRNINLLFTKFKEHNPHPRIELKYTNSFTLLIAIVLSARTTDVSVNKITDKLFEVADT
CCCCCEEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
PRKMLDLGEKGLKNYINTIGLYNSKSKNIIALSGIIINQHNSNVPLDFNTLVALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHEEECCCCCCC
SANVFLNTWLNLPTVAVDTHVFRVSNRIGLVKESNVLKTEDALLNVIPKKWLLYAHHWLV
CCCEEEEECCCCCEEEEHHEEEEECCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHEEE
LHGRYVCKSRKPLCSQCVVQDLCEYESKSL
EECHHEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]