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The map label for this gene is tsaA [H]

Identifier: 88658172

GI number: 88658172

Start: 744918

End: 745535

Strand: Direct

Name: tsaA [H]

Synonym: ECH_0734

Alternate gene names: 88658172

Gene position: 744918-745535 (Clockwise)

Preceding gene: 88657679

Following gene: 88658008

Centisome position: 63.33

GC content: 33.66

Gene sequence:

>618_bases
ATGAATCTCATAACAAAACAAGCTATTGACTTTACAGCAAAAGCCGTTATGCCAGATGACAAAATATGTGAATTTAATTT
GAAAGAATATGTCAGTGGCAAATATGCTATTTTGATCTTCTACCCTAGAGATTTTACGTTCGTATGCCCTACAGAAATTA
TATCTCTACACAATAGAATACCTCAATTTATAGAAAAAAACACTGAAATTATTGGTATTAGTACAGACTCAGAGTTTTGT
CATAGCCATTGGCGTAACATTAGCATAAACAAAGGTGGAATTGATAAAATTAGTTTCCCACTAATCGCAGATGACTCACA
TTTTATCTCTAATAGCTATGGGGTATTGCATAACAATAGTATTGCATTAAGGGCAACCTTTGTCATCGATGATAATTTCA
TAGTACGCCATCAATCGATAAATGACCTTCCTATAGGTAGAAACGTAGATGAGCTTTTAAGAATAATAGATGCAATAAAA
CATCATCAGGTTCATGGAGAAGTATGCCCAGCTGGATGGATGAAAGGAGATCCAGCAATGGAAGCATCACAACATGGTAT
TGAAGATTACTTAAAATCACACATAGATCACCTTGAAAAAAGTATTATGGATGAATAA

Upstream 100 bases:

>100_bases
TTATATGACAATTTATATCATAAATTATAATTACAAAACTATTAAATTGTAGTATTAAGTACTATAACGATTGTTGCTAT
ATAATTAACTAGGTAATTTT

Downstream 100 bases:

>100_bases
ATGACACAAACATATAATACAAAAGTTTTAATTATAGGATCTGGAGCAGCAGGTTGTACTGCTGCTATATATGCTGCTCG
TGCAAACTTAAAACCTATAT

Product: anti-oxidant AhpCTSA family protein

Products: NA

Alternate protein names: 26 kDa antigen; Thioredoxin reductase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MNLITKQAIDFTAKAVMPDDKICEFNLKEYVSGKYAILIFYPRDFTFVCPTEIISLHNRIPQFIEKNTEIIGISTDSEFC
HSHWRNISINKGGIDKISFPLIADDSHFISNSYGVLHNNSIALRATFVIDDNFIVRHQSINDLPIGRNVDELLRIIDAIK
HHQVHGEVCPAGWMKGDPAMEASQHGIEDYLKSHIDHLEKSIMDE

Sequences:

>Translated_205_residues
MNLITKQAIDFTAKAVMPDDKICEFNLKEYVSGKYAILIFYPRDFTFVCPTEIISLHNRIPQFIEKNTEIIGISTDSEFC
HSHWRNISINKGGIDKISFPLIADDSHFISNSYGVLHNNSIALRATFVIDDNFIVRHQSINDLPIGRNVDELLRIIDAIK
HHQVHGEVCPAGWMKGDPAMEASQHGIEDYLKSHIDHLEKSIMDE
>Mature_205_residues
MNLITKQAIDFTAKAVMPDDKICEFNLKEYVSGKYAILIFYPRDFTFVCPTEIISLHNRIPQFIEKNTEIIGISTDSEFC
HSHWRNISINKGGIDKISFPLIADDSHFISNSYGVLHNNSIALRATFVIDDNFIVRHQSINDLPIGRNVDELLRIIDAIK
HHQVHGEVCPAGWMKGDPAMEASQHGIEDYLKSHIDHLEKSIMDE

Specific function: Directly Reduces Organic Hydroperoxides In Its Reduced Dithiol Form. [C]

COG id: COG0450

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Homo sapiens, GI4505591, Length=188, Percent_Identity=43.0851063829787, Blast_Score=175, Evalue=2e-44,
Organism=Homo sapiens, GI32455266, Length=188, Percent_Identity=43.0851063829787, Blast_Score=175, Evalue=2e-44,
Organism=Homo sapiens, GI32455264, Length=188, Percent_Identity=43.0851063829787, Blast_Score=175, Evalue=2e-44,
Organism=Homo sapiens, GI32189392, Length=192, Percent_Identity=43.75, Blast_Score=171, Evalue=3e-43,
Organism=Homo sapiens, GI5453549, Length=183, Percent_Identity=45.9016393442623, Blast_Score=164, Evalue=4e-41,
Organism=Homo sapiens, GI5802974, Length=190, Percent_Identity=39.4736842105263, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI32483377, Length=189, Percent_Identity=39.6825396825397, Blast_Score=157, Evalue=5e-39,
Organism=Homo sapiens, GI33188454, Length=81, Percent_Identity=50.6172839506173, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4758638, Length=162, Percent_Identity=26.5432098765432, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1786822, Length=155, Percent_Identity=32.9032258064516, Blast_Score=101, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193204376, Length=193, Percent_Identity=43.5233160621762, Blast_Score=166, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI32565831, Length=193, Percent_Identity=43.5233160621762, Blast_Score=166, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17554494, Length=160, Percent_Identity=41.875, Blast_Score=144, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25153706, Length=163, Percent_Identity=25.1533742331288, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323613, Length=191, Percent_Identity=40.3141361256544, Blast_Score=152, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320661, Length=191, Percent_Identity=37.696335078534, Blast_Score=141, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6319407, Length=168, Percent_Identity=26.7857142857143, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24656348, Length=175, Percent_Identity=46.2857142857143, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17864676, Length=175, Percent_Identity=46.2857142857143, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17738015, Length=171, Percent_Identity=48.5380116959064, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17157991, Length=194, Percent_Identity=40.7216494845361, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24641739, Length=194, Percent_Identity=40.7216494845361, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21357347, Length=193, Percent_Identity=39.3782383419689, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24581278, Length=178, Percent_Identity=30.3370786516854, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI17975518, Length=163, Percent_Identity=30.0613496932515, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24652436, Length=163, Percent_Identity=29.4478527607362, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652434, Length=163, Percent_Identity=29.4478527607362, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2250 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 6040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1580 Molecules/Cell In: Stationary-Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000866
- InterPro:   IPR019479
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF10417 1-cysPrx_C; PF00578 AhpC-TSA [H]

EC number: =1.11.1.15 [H]

Molecular weight: Translated: 23362; Mature: 23362

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLITKQAIDFTAKAVMPDDKICEFNLKEYVSGKYAILIFYPRDFTFVCPTEIISLHNRI
CCCCHHHHHHHHHHCCCCCCCEEECCHHHHCCCCEEEEEEECCCCEEECHHHHHHHHHHH
PQFIEKNTEIIGISTDSEFCHSHWRNISINKGGIDKISFPLIADDSHFISNSYGVLHNNS
HHHHHCCCEEEEEECCHHHHHHHCCEEEECCCCCCEEECEEEECCCCCCCCCCCEEECCC
IALRATFVIDDNFIVRHQSINDLPIGRNVDELLRIIDAIKHHQVHGEVCPAGWMKGDPAM
EEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EASQHGIEDYLKSHIDHLEKSIMDE
HHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLITKQAIDFTAKAVMPDDKICEFNLKEYVSGKYAILIFYPRDFTFVCPTEIISLHNRI
CCCCHHHHHHHHHHCCCCCCCEEECCHHHHCCCCEEEEEEECCCCEEECHHHHHHHHHHH
PQFIEKNTEIIGISTDSEFCHSHWRNISINKGGIDKISFPLIADDSHFISNSYGVLHNNS
HHHHHCCCEEEEEECCHHHHHHHCCEEEECCCCCCEEECEEEECCCCCCCCCCCEEECCC
IALRATFVIDDNFIVRHQSINDLPIGRNVDELLRIIDAIKHHQVHGEVCPAGWMKGDPAM
EEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EASQHGIEDYLKSHIDHLEKSIMDE
HHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682; 1987145 [H]