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The map label for this gene is argG [H]

Identifier: 88658029

GI number: 88658029

Start: 689784

End: 690965

Strand: Direct

Name: argG [H]

Synonym: ECH_0680

Alternate gene names: 88658029

Gene position: 689784-690965 (Clockwise)

Preceding gene: 88657769

Following gene: 88657741

Centisome position: 58.64

GC content: 32.57

Gene sequence:

>1182_bases
ATGAAAAAAATAGTATTAGCTTATTCAGGGGGATTAGATACCTCAGTTATATTAAAATGGCTCCAAGAAAATTATAATTG
TGAAGTAGTAGTATTTACTGCAGATTTAGGTCAAAATGATGATATGTCTGCAATAAGACAAAAGGCAGCTGCATCAAATG
TAAAAGAAATTTTTATAGAAGACTTAAAAGAAGAATTTGTAAAAGATTTCGTATTCCCCATGTTTAGAGCAAATACTGTA
TATGAAGGATATTACTTATTGGGAACATCTATAGCAAGGCCATTAATAGCTAAACGACAAATTGAAATAGCTCACCTTAC
AGGAGCAGATGCTGTAGCACATGGAGCAACTGGAAAAGGAAATGACCAGATTAGATTTGAATTTGGATATTATTCATGCG
ATCCAAATATAAAGGTAATAGCACCTTGGCGTCAGTGGGAATTGACTTCAAGAAATAGTTTAATAGAATATGCAAAAAAG
CATGGTATTGATGTTCCTTTAGATAAAGCAAGTGAACCACCATATTCTATAGATGCAAACCTATTACATACCTCATACGA
AGGCAAATCTTTAGAAGATCCATATATTGAACCTGATTATACAATGCTCTCCAAATCAGTAATTCCAGAATTAGCTAGTG
ATACTCCAGAATATATAGAAATTTCCTTTAAAAACGGAGATCCACATGCAATAAATAATGTACCCCTTTCACCTGCAAAT
CTATTACAACAATTGAATATAATAGGTGGTAGACATGGTATAGGAATTATTGACATAGTAGAAAATCGCTATATAGGCAT
AAAATCAAGAGGAGTATACGAAACTCCTGGAGGAACAATTCTTTTACACGCACACCGTGCTATAGAAAGTATAACTTTAG
ACAGAGAAGCAGCACACCTTAAGGATGAAATAATGCCAAGATATGCTAAACTAATATATAATGGATATTGGTGGACTACT
GAAAGAAAGATGTTACAAGCACTTATTGATAAATCACAAGACAAAGTAAATGGAGTAGTACGCTTAAAATTATACAAAGG
ATCTGTTATGGTTGTAGGACGAACATCAGAAAATAGCTTATATTCACATAACCTAGCAAGCTTTGACTCATCAGGAAATT
ATAATCATACAGACGCAGAAGGATTTATTAAAATAAATTCTCTAAGATTAAGAAATAGCTAA

Upstream 100 bases:

>100_bases
ATCTTATATTATTGCACTATTACTAATCAATCAGTAATTAAGTTGTCACCCCAATCAAAATACAATAAAATCTAGTCTTT
AATTAATATTTCCATTATCT

Downstream 100 bases:

>100_bases
CATTTTACTTTAATATTGTACCTTTAAAATAACAATATTCATCTTAAGTAGCATTAAGTCCAAATCAAAGTATTATCACT
AATATATTTACCCTAAGATT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADLGQNDDMSAIRQKAAASNVKEIFIEDLKEEFVKDFVFPMFRANTV
YEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKGNDQIRFEFGYYSCDPNIKVIAPWRQWELTSRNSLIEYAKK
HGIDVPLDKASEPPYSIDANLLHTSYEGKSLEDPYIEPDYTMLSKSVIPELASDTPEYIEISFKNGDPHAINNVPLSPAN
LLQQLNIIGGRHGIGIIDIVENRYIGIKSRGVYETPGGTILLHAHRAIESITLDREAAHLKDEIMPRYAKLIYNGYWWTT
ERKMLQALIDKSQDKVNGVVRLKLYKGSVMVVGRTSENSLYSHNLASFDSSGNYNHTDAEGFIKINSLRLRNS

Sequences:

>Translated_393_residues
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADLGQNDDMSAIRQKAAASNVKEIFIEDLKEEFVKDFVFPMFRANTV
YEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKGNDQIRFEFGYYSCDPNIKVIAPWRQWELTSRNSLIEYAKK
HGIDVPLDKASEPPYSIDANLLHTSYEGKSLEDPYIEPDYTMLSKSVIPELASDTPEYIEISFKNGDPHAINNVPLSPAN
LLQQLNIIGGRHGIGIIDIVENRYIGIKSRGVYETPGGTILLHAHRAIESITLDREAAHLKDEIMPRYAKLIYNGYWWTT
ERKMLQALIDKSQDKVNGVVRLKLYKGSVMVVGRTSENSLYSHNLASFDSSGNYNHTDAEGFIKINSLRLRNS
>Mature_393_residues
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADLGQNDDMSAIRQKAAASNVKEIFIEDLKEEFVKDFVFPMFRANTV
YEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKGNDQIRFEFGYYSCDPNIKVIAPWRQWELTSRNSLIEYAKK
HGIDVPLDKASEPPYSIDANLLHTSYEGKSLEDPYIEPDYTMLSKSVIPELASDTPEYIEISFKNGDPHAINNVPLSPAN
LLQQLNIIGGRHGIGIIDIVENRYIGIKSRGVYETPGGTILLHAHRAIESITLDREAAHLKDEIMPRYAKLIYNGYWWTT
ERKMLQALIDKSQDKVNGVVRLKLYKGSVMVVGRTSENSLYSHNLASFDSSGNYNHTDAEGFIKINSLRLRNS

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=397, Percent_Identity=48.1108312342569, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI16950633, Length=397, Percent_Identity=48.1108312342569, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1789563, Length=410, Percent_Identity=27.0731707317073, Blast_Score=128, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324514, Length=405, Percent_Identity=42.7160493827161, Blast_Score=318, Evalue=1e-87,
Organism=Drosophila melanogaster, GI21358151, Length=402, Percent_Identity=43.0348258706468, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44169; Mature: 44169

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADLGQNDDMSAIRQKAAASNVKEIFIE
CCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
DLKEEFVKDFVFPMFRANTVYEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKG
HHHHHHHHHHHHHHHHCCEEECCEEEECHHHHHHHHHHCEEEEEEECCCHHHHCCCCCCC
NDQIRFEFGYYSCDPNIKVIAPWRQWELTSRNSLIEYAKKHGIDVPLDKASEPPYSIDAN
CCEEEEEEEEEEECCCCEEEECCHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
LLHTSYEGKSLEDPYIEPDYTMLSKSVIPELASDTPEYIEISFKNGDPHAINNVPLSPAN
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHH
LLQQLNIIGGRHGIGIIDIVENRYIGIKSRGVYETPGGTILLHAHRAIESITLDREAAHL
HHHHHHHCCCCCCCEEEEEECCCEEEEECCCCEECCCCEEEEEEHHHHHHHCCCHHHHHH
KDEIMPRYAKLIYNGYWWTTERKMLQALIDKSQDKVNGVVRLKLYKGSVMVVGRTSENSL
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEEEECCCCCC
YSHNLASFDSSGNYNHTDAEGFIKINSLRLRNS
HHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADLGQNDDMSAIRQKAAASNVKEIFIE
CCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
DLKEEFVKDFVFPMFRANTVYEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKG
HHHHHHHHHHHHHHHHCCEEECCEEEECHHHHHHHHHHCEEEEEEECCCHHHHCCCCCCC
NDQIRFEFGYYSCDPNIKVIAPWRQWELTSRNSLIEYAKKHGIDVPLDKASEPPYSIDAN
CCEEEEEEEEEEECCCCEEEECCHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
LLHTSYEGKSLEDPYIEPDYTMLSKSVIPELASDTPEYIEISFKNGDPHAINNVPLSPAN
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHH
LLQQLNIIGGRHGIGIIDIVENRYIGIKSRGVYETPGGTILLHAHRAIESITLDREAAHL
HHHHHHHCCCCCCCEEEEEECCCEEEEECCCCEECCCCEEEEEEHHHHHHHCCCHHHHHH
KDEIMPRYAKLIYNGYWWTTERKMLQALIDKSQDKVNGVVRLKLYKGSVMVVGRTSENSL
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEEEECCCCCC
YSHNLASFDSSGNYNHTDAEGFIKINSLRLRNS
HHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA