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The map label for this gene is dus [H]

Identifier: 88657985

GI number: 88657985

Start: 764498

End: 765451

Strand: Direct

Name: dus [H]

Synonym: ECH_0758

Alternate gene names: 88657985

Gene position: 764498-765451 (Clockwise)

Preceding gene: 88658405

Following gene: 88658478

Centisome position: 64.99

GC content: 32.18

Gene sequence:

>954_bases
ATGGACATTAGCCAATTTATATCAGATTATCCAGTAATATTAGCACCAATGTCTGGTGTAACTGACTTTCCATTTCGTTA
CCTTGTAAAGAGCCTTGGGGCTGGATTATTAGTATCAGAAATGATAGCAAGCCGTGCAATGATTATACAAACTAAACAAA
GTTTACAAAAATCACAAATTCAAATTAACACTGCAGTACAACTTGCCGGATGTGATCCAACAATAATGGCAGACGCTGCT
AAACTCAATGAAGATATGGGAGCACAAATCATAGATATAAATTTTGGATGTCCTGTAAAAAAAGTAGTCAACAGTTACGC
TGGTTCAGCATTAATGAAAGATGAAACTACAGCTGCAAAAATTCTAGAATCTGTAGTAAAAGCAGTAAAAATTCCAGTAA
CACTAAAAATGCGCACTGGATGGGACAACAATAACTTAAATTCACCTAAATTAGCTAAGATTGCTGAAGAAACTGGAATA
AAAATGATAACAATACATGGCAGAACAAGAACACAAATGTTTACTGGGAAGGCAGATTGGAAATTTATCAGAAATATTAA
AAAATGTGTAAAAATTCCTGTCATAGTTAACGGAGATATAAAAAACTTAAATGATATAAAAACATCACTAGAAGAATCAC
AAGCAGATGGAATCATGATAGGAAGAGGAGCATACGGCAAGCCATGGTTTATCAATCAAGCTTTACACTTCATAAAATCA
GGCACAATTTTACCTGATCCTTCACCATCAGAAAAATTAAAAATTATCACAAATCATTATGATCATATGATAGAATACTA
TGGAAAAGATACTGGAGTAAAAATATCAAAAAAACACATAGGATGGTATAGTTCTTCTCTAGAGAATTCATCCGAGTTTA
GATCTCAAGTTAACTGTTCTAATGATTACAACTTTATCAAAGACAAAATAACAGATTTTTTCTCACAAAATTAA

Upstream 100 bases:

>100_bases
AACCAATAACAAAAGTAGATCCTTTCTATAAAATTATATATTATATTGATAGTGTTTTTTACTTACATCAAAATATAATA
AACTCAAAATATTAAAACAT

Downstream 100 bases:

>100_bases
TGTTGTACTGAAAACGCTTGTATACAAAATAACAGTAGATAATTACCACTTAATGTATACAAATGGCAACCAACAACTAA
TAACATATTACAAATTATTT

Product: putative tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MDISQFISDYPVILAPMSGVTDFPFRYLVKSLGAGLLVSEMIASRAMIIQTKQSLQKSQIQINTAVQLAGCDPTIMADAA
KLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDETTAAKILESVVKAVKIPVTLKMRTGWDNNNLNSPKLAKIAEETGI
KMITIHGRTRTQMFTGKADWKFIRNIKKCVKIPVIVNGDIKNLNDIKTSLEESQADGIMIGRGAYGKPWFINQALHFIKS
GTILPDPSPSEKLKIITNHYDHMIEYYGKDTGVKISKKHIGWYSSSLENSSEFRSQVNCSNDYNFIKDKITDFFSQN

Sequences:

>Translated_317_residues
MDISQFISDYPVILAPMSGVTDFPFRYLVKSLGAGLLVSEMIASRAMIIQTKQSLQKSQIQINTAVQLAGCDPTIMADAA
KLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDETTAAKILESVVKAVKIPVTLKMRTGWDNNNLNSPKLAKIAEETGI
KMITIHGRTRTQMFTGKADWKFIRNIKKCVKIPVIVNGDIKNLNDIKTSLEESQADGIMIGRGAYGKPWFINQALHFIKS
GTILPDPSPSEKLKIITNHYDHMIEYYGKDTGVKISKKHIGWYSSSLENSSEFRSQVNCSNDYNFIKDKITDFFSQN
>Mature_317_residues
MDISQFISDYPVILAPMSGVTDFPFRYLVKSLGAGLLVSEMIASRAMIIQTKQSLQKSQIQINTAVQLAGCDPTIMADAA
KLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDETTAAKILESVVKAVKIPVTLKMRTGWDNNNLNSPKLAKIAEETGI
KMITIHGRTRTQMFTGKADWKFIRNIKKCVKIPVIVNGDIKNLNDIKTSLEESQADGIMIGRGAYGKPWFINQALHFIKS
GTILPDPSPSEKLKIITNHYDHMIEYYGKDTGVKISKKHIGWYSSSLENSSEFRSQVNCSNDYNFIKDKITDFFSQN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=287, Percent_Identity=31.3588850174216, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI239788462, Length=287, Percent_Identity=31.0104529616725, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI40807366, Length=223, Percent_Identity=26.0089686098655, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=29.2792792792793, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI8923374, Length=252, Percent_Identity=28.968253968254, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1789660, Length=319, Percent_Identity=40.4388714733542, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=31.1688311688312, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693211, Length=228, Percent_Identity=30.2631578947368, Blast_Score=98, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=278, Percent_Identity=29.136690647482, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17507177, Length=229, Percent_Identity=27.0742358078603, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=220, Percent_Identity=26.8181818181818, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17510279, Length=236, Percent_Identity=29.2372881355932, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=288, Percent_Identity=27.0833333333333, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=29.8701298701299, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=26.7241379310345, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585320, Length=288, Percent_Identity=29.8611111111111, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24580595, Length=223, Percent_Identity=31.390134529148, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19920448, Length=223, Percent_Identity=31.390134529148, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45549423, Length=240, Percent_Identity=29.1666666666667, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19921524, Length=236, Percent_Identity=26.271186440678, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35255; Mature: 35255

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISQFISDYPVILAPMSGVTDFPFRYLVKSLGAGLLVSEMIASRAMIIQTKQSLQKSQI
CCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHE
QINTAVQLAGCDPTIMADAAKLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDETTAAK
EEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCHHCCCHHHHH
ILESVVKAVKIPVTLKMRTGWDNNNLNSPKLAKIAEETGIKMITIHGRTRTQMFTGKADW
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCCEEEEEECCCHH
KFIRNIKKCVKIPVIVNGDIKNLNDIKTSLEESQADGIMIGRGAYGKPWFINQALHFIKS
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHC
GTILPDPSPSEKLKIITNHYDHMIEYYGKDTGVKISKKHIGWYSSSLENSSEFRSQVNCS
CCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEHHHCCCHHHHCCCHHHHHHHCCCC
NDYNFIKDKITDFFSQN
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDISQFISDYPVILAPMSGVTDFPFRYLVKSLGAGLLVSEMIASRAMIIQTKQSLQKSQI
CCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHE
QINTAVQLAGCDPTIMADAAKLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDETTAAK
EEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCHHCCCHHHHH
ILESVVKAVKIPVTLKMRTGWDNNNLNSPKLAKIAEETGIKMITIHGRTRTQMFTGKADW
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCCEEEEEECCCHH
KFIRNIKKCVKIPVIVNGDIKNLNDIKTSLEESQADGIMIGRGAYGKPWFINQALHFIKS
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHC
GTILPDPSPSEKLKIITNHYDHMIEYYGKDTGVKISKKHIGWYSSSLENSSEFRSQVNCS
CCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEHHHCCCHHHHCCCHHHHHHHCCCC
NDYNFIKDKITDFFSQN
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA