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The map label for this gene is thiE [H]

Identifier: 88657902

GI number: 88657902

Start: 918668

End: 919402

Strand: Reverse

Name: thiE [H]

Synonym: ECH_0893

Alternate gene names: 88657902

Gene position: 919402-918668 (Counterclockwise)

Preceding gene: 88658617

Following gene: 88657974

Centisome position: 78.16

GC content: 31.43

Gene sequence:

>735_bases
TTGCAAAAGGATGCTTTAATTATAGCAAGAGCTTCTGCTAATCAATCTGTTGAAGATGCTAGTTTTTTAGATGAAAGGTA
TTTCCCTATTATTACGCTTAATAAAGATTATTATTTTGATAAAAATTTTCATCCTATGCTTGATCCTGTAGGTTTTTACC
AAATAGTGCCAGATCTATTTTGGTTGAAGTATGTAATTAATTTAGGGGTTAAAGTAGTACAGCTGAGAATTAAAAATGAA
CCAATAGAAGAAGTAGAGTATAAAATAAAGGAAGGTGTACATATTGCAAATCAAAATGGTGTAAAATTGTTTGTGAATGA
TTATTGGCAGTTTGCAGTAAAGTATAAAGCTTATGGAGTGCATTTAGGACAGGAGGACTTGAGGAGTGCAAACTTTAATG
AAATATATAATGCAGGATTGAGACTAGGTATTAGTACCCATTGTTATCATGAATTAGCAATAGCGAAATATATACGTCCT
TCATATATTGCTTTTGGCCCTATTTTTCCTACTACATTAAAAAACATGAATTTTATGCCACAGGGTACAGATTTACTTAA
TTATTGGGTTAAGAATTTACCTTATAAAATTGTGGCTATTGGTGGCATTAATTTGTCAAATGTGGATTCAGTAATTGGAA
CTGGTGTTGACGGCATAGCTGTTATATCTGCTGTGTTAAATAGTGAATATCCTGATAAAGTGGTACATGAGTTTATACAA
AAATGCCAAATCTAG

Upstream 100 bases:

>100_bases
ATGGTTTTTATATAACGGAGTGTCATGTAATGGTAAAGAAAATTTCTTGTCTGCATATAAAGCATCTGTGTTAGTAAGTA
AGTGTTTAAATGCTGCTGAC

Downstream 100 bases:

>100_bases
CTCGCAGTTATAAGTAATTTGGTTATAACGGTCTTATTATTGAACGTGTACTCGGTTAAGTTTGTTGTTCTGTCTTGTGT
TTTATAGTAGATGTATCTTT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MQKDALIIARASANQSVEDASFLDERYFPIITLNKDYYFDKNFHPMLDPVGFYQIVPDLFWLKYVINLGVKVVQLRIKNE
PIEEVEYKIKEGVHIANQNGVKLFVNDYWQFAVKYKAYGVHLGQEDLRSANFNEIYNAGLRLGISTHCYHELAIAKYIRP
SYIAFGPIFPTTLKNMNFMPQGTDLLNYWVKNLPYKIVAIGGINLSNVDSVIGTGVDGIAVISAVLNSEYPDKVVHEFIQ
KCQI

Sequences:

>Translated_244_residues
MQKDALIIARASANQSVEDASFLDERYFPIITLNKDYYFDKNFHPMLDPVGFYQIVPDLFWLKYVINLGVKVVQLRIKNE
PIEEVEYKIKEGVHIANQNGVKLFVNDYWQFAVKYKAYGVHLGQEDLRSANFNEIYNAGLRLGISTHCYHELAIAKYIRP
SYIAFGPIFPTTLKNMNFMPQGTDLLNYWVKNLPYKIVAIGGINLSNVDSVIGTGVDGIAVISAVLNSEYPDKVVHEFIQ
KCQI
>Mature_244_residues
MQKDALIIARASANQSVEDASFLDERYFPIITLNKDYYFDKNFHPMLDPVGFYQIVPDLFWLKYVINLGVKVVQLRIKNE
PIEEVEYKIKEGVHIANQNGVKLFVNDYWQFAVKYKAYGVHLGQEDLRSANFNEIYNAGLRLGISTHCYHELAIAKYIRP
SYIAFGPIFPTTLKNMNFMPQGTDLLNYWVKNLPYKIVAIGGINLSNVDSVIGTGVDGIAVISAVLNSEYPDKVVHEFIQ
KCQI

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=191, Percent_Identity=44.5026178010471, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 27850; Mature: 27850

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKDALIIARASANQSVEDASFLDERYFPIITLNKDYYFDKNFHPMLDPVGFYQIVPDLF
CCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHH
WLKYVINLGVKVVQLRIKNEPIEEVEYKIKEGVHIANQNGVKLFVNDYWQFAVKYKAYGV
HHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCEEEECCCCEEEEEEHHHHHHEEEEEEEE
HLGQEDLRSANFNEIYNAGLRLGISTHCYHELAIAKYIRPSYIAFGPIFPTTLKNMNFMP
ECCHHHHHCCCHHHHHHHHHEECCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHCCCCCC
QGTDLLNYWVKNLPYKIVAIGGINLSNVDSVIGTGVDGIAVISAVLNSEYPDKVVHEFIQ
CCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
KCQI
HHCC
>Mature Secondary Structure
MQKDALIIARASANQSVEDASFLDERYFPIITLNKDYYFDKNFHPMLDPVGFYQIVPDLF
CCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHH
WLKYVINLGVKVVQLRIKNEPIEEVEYKIKEGVHIANQNGVKLFVNDYWQFAVKYKAYGV
HHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCEEEECCCCEEEEEEHHHHHHEEEEEEEE
HLGQEDLRSANFNEIYNAGLRLGISTHCYHELAIAKYIRPSYIAFGPIFPTTLKNMNFMP
ECCHHHHHCCCHHHHHHHHHEECCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHCCCCCC
QGTDLLNYWVKNLPYKIVAIGGINLSNVDSVIGTGVDGIAVISAVLNSEYPDKVVHEFIQ
CCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
KCQI
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]