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The map label for this gene is puuA [H]

Identifier: 88657811

GI number: 88657811

Start: 1007888

End: 1008685

Strand: Direct

Name: puuA [H]

Synonym: ECH_0983

Alternate gene names: 88657811

Gene position: 1007888-1008685 (Clockwise)

Preceding gene: 88658445

Following gene: 88658448

Centisome position: 85.69

GC content: 29.57

Gene sequence:

>798_bases
ATGTTAAACTATACACTAGACTATCTCAGTACCAAACTTAACATTATTCCCTTAATAGGGATAGAATTAGAATTTTACTT
TGATAATATAGACCCAAATAATATCAGCCCATTAATTAGCAACATTCAAGACAAAATATCTTCTCTTAACTGTAATATAA
CAAAAGAACAAGATAATCTACAATATGAAATACAAACATCAACAACTACTAACATTCCTAATTTTATTATTGAGCTTGAT
CTTATAAAAGAAATTTTAGAAAACAACACAAAACACTTTGGAGGGAGCATTAATTTCTCTGCAAAACCATATTTAGATAA
ACCAGGAAGTGCATTTCACATACATATAAATTTACTTGATTTTCATAATAATAATTTGTTTACTAGCCAAAATAACAAAA
TGAGTGACCATCTATCCTACAGCATAGGGGGATTATGCTCTTTAATGAAAAAACATATGATTTTTTTTGCTCCAAATAAC
AACTCCTACTTAAGATATATATATGCTGACATAGACACCCCAACAACTATAAGTTGGGGAGGAAATAATAGGAGTACTTC
TATCCGTATACCAAGTACCAGTACAGACCCAACAAAATGCAGAATAGAACACAGAGTACCTGGAGCAGACTGTAACTATA
AACAAGCTATAACATCAGTTCTGCAAGGAATAATTTATGGAATAGAGAAAAAAATACAGCCACCACAAAAAATTTATGGT
ATATCCTCAGACATACAATATAATCTTGAAAAATTACCATTGAGTTTAAACGAATCAATAAAATATAACCTAAAGTAG

Upstream 100 bases:

>100_bases
TTTAGGTCTCACATTGTTTATCATAACATGGCTTCTTAATTTACTAGCACTACACATTACAAAAAAAGCAACAAAATCTA
TTAAAATTCACTAATTCCAT

Downstream 100 bases:

>100_bases
AGAACTTACAATAAATTGTTATTACAAATAGACTATAAATTAATTTTACCAAAAATAGTTTATGTAATAGAGAAAAAACA
CCACAAAAAATTTACAGAAT

Product: glutamine synthetase domain-containing protein

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLNYTLDYLSTKLNIIPLIGIELEFYFDNIDPNNISPLISNIQDKISSLNCNITKEQDNLQYEIQTSTTTNIPNFIIELD
LIKEILENNTKHFGGSINFSAKPYLDKPGSAFHIHINLLDFHNNNLFTSQNNKMSDHLSYSIGGLCSLMKKHMIFFAPNN
NSYLRYIYADIDTPTTISWGGNNRSTSIRIPSTSTDPTKCRIEHRVPGADCNYKQAITSVLQGIIYGIEKKIQPPQKIYG
ISSDIQYNLEKLPLSLNESIKYNLK

Sequences:

>Translated_265_residues
MLNYTLDYLSTKLNIIPLIGIELEFYFDNIDPNNISPLISNIQDKISSLNCNITKEQDNLQYEIQTSTTTNIPNFIIELD
LIKEILENNTKHFGGSINFSAKPYLDKPGSAFHIHINLLDFHNNNLFTSQNNKMSDHLSYSIGGLCSLMKKHMIFFAPNN
NSYLRYIYADIDTPTTISWGGNNRSTSIRIPSTSTDPTKCRIEHRVPGADCNYKQAITSVLQGIIYGIEKKIQPPQKIYG
ISSDIQYNLEKLPLSLNESIKYNLK
>Mature_265_residues
MLNYTLDYLSTKLNIIPLIGIELEFYFDNIDPNNISPLISNIQDKISSLNCNITKEQDNLQYEIQTSTTTNIPNFIIELD
LIKEILENNTKHFGGSINFSAKPYLDKPGSAFHIHINLLDFHNNNLFTSQNNKMSDHLSYSIGGLCSLMKKHMIFFAPNN
NSYLRYIYADIDTPTTISWGGNNRSTSIRIPSTSTDPTKCRIEHRVPGADCNYKQAITSVLQGIIYGIEKKIQPPQKIYG
ISSDIQYNLEKLPLSLNESIKYNLK

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081870, Length=262, Percent_Identity=25.9541984732824, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790301, Length=188, Percent_Identity=28.7234042553192, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 30149; Mature: 30149

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNYTLDYLSTKLNIIPLIGIELEFYFDNIDPNNISPLISNIQDKISSLNCNITKEQDNL
CCEEEEHHHHCCEEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCE
QYEIQTSTTTNIPNFIIELDLIKEILENNTKHFGGSINFSAKPYLDKPGSAFHIHINLLD
EEEEEECCCCCCCHHEEEHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCEEEEEEEEEE
FHNNNLFTSQNNKMSDHLSYSIGGLCSLMKKHMIFFAPNNNSYLRYIYADIDTPTTISWG
EECCEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEECCCCEEEEEC
GNNRSTSIRIPSTSTDPTKCRIEHRVPGADCNYKQAITSVLQGIIYGIEKKIQPPQKIYG
CCCCCEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCC
ISSDIQYNLEKLPLSLNESIKYNLK
CCCCCEECHHHCCEECCCCEEEECC
>Mature Secondary Structure
MLNYTLDYLSTKLNIIPLIGIELEFYFDNIDPNNISPLISNIQDKISSLNCNITKEQDNL
CCEEEEHHHHCCEEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCE
QYEIQTSTTTNIPNFIIELDLIKEILENNTKHFGGSINFSAKPYLDKPGSAFHIHINLLD
EEEEEECCCCCCCHHEEEHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCEEEEEEEEEE
FHNNNLFTSQNNKMSDHLSYSIGGLCSLMKKHMIFFAPNNNSYLRYIYADIDTPTTISWG
EECCEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEECCCCEEEEEC
GNNRSTSIRIPSTSTDPTKCRIEHRVPGADCNYKQAITSVLQGIIYGIEKKIQPPQKIYG
CCCCCEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCC
ISSDIQYNLEKLPLSLNESIKYNLK
CCCCCEECHHHCCEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]