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The map label for this gene is purK [H]

Identifier: 88657693

GI number: 88657693

Start: 1054606

End: 1055691

Strand: Direct

Name: purK [H]

Synonym: ECH_1034

Alternate gene names: 88657693

Gene position: 1054606-1055691 (Clockwise)

Preceding gene: 88658188

Following gene: 88657748

Centisome position: 89.66

GC content: 31.49

Gene sequence:

>1086_bases
ATGCTACACGATTCATACATCGGCCCTGGATCAACTATTGGGATTATAGGTGGAGGACAGTTAGGAAAAATGATATCCAT
TGCTGCAGCAAACTTAGGATATAAAACACATTTATTAACTAATAACCCGGATGATCCATCAGTCTACATTACTAACAGTG
CAACTATATCACACAATTATCAAAATACAGAATCATTGCTTGAGTTTGCATCTAATGTCGACATTGCAACCTTAGAATTT
GAGAACATTCCTACTACTACTATAGATATATTATCACAAAAAATCAAAGTTTATCCAGGAAAAGAAGCTTTATACATTTC
CCAAAATAGAATAAGAGAAAAACAGCACATAAGAAATTTAGGAATCAAAACTTCAGATTTTAGAGTAATTGATAACTACA
ACAGTTTAGTCAAAAATACTATAGAACTAGGATATCCCACCTTACTTAAGACCACAGAATTAGGCTACGATGGAAAAGGC
CAGTATATAATAAAACAACAAGATGATTTAAGTGCTTTATCAACTCTCAATTGGGACCAATCATATATTTTAGAAAAATT
TGTCAAAATTTATAAAGAAATATCTGTTATAATAACAAAAAGCATCAGTGGTTCTATAGAATTTTTTCCAACTGCGGAAA
ACTGTCATACTGATGGTATTTTAACCACATCATCAGTACCAGCCCTAATCTCTCAAGAGATAAATGTACAAGCACAAAAA
ATTGCTTTACAAATTGCAGAATCTATTAATTTAGTAGGTTTATTAGCAGTGGAATTTTTCATAACAGATACACAAGAACT
TATAGTTAATGAAATAGCTCCCCGCCCTCACAACTCTGGACATTGGAGCTTAGATGCTTGTAACATCAGCCAATTTGAAC
AATTAATAAGAGCAATATGTGGATTACCTTTAAAGCCTGTAAAATTACTTTTTCCATGTATTATGAATAACATATTAGGA
GATAATATACACAACTATTATAAACATGAAACCAAGGTTAATGAAAACTTATACATATACGGCAAGAAAAAGGCCACTAA
AAACAGAAAAATGGGCCACATTAACACATTAAAATTCAACCAGTAA

Upstream 100 bases:

>100_bases
CAGTGTTACAAAAATTACACACCTTATTATTTAATTGACTTGTTTTTTTGAGGCAGATTATTATTAACTTTGAAAATACA
ACAACAGTAAATTGATCACT

Downstream 100 bases:

>100_bases
CCGGAGCAACTATCATTAGAAAGTTTAGTTTCACAATACAAATATTCACGAATTTATAAATGAAATAATAAACTACACAG
TACACCAAAAAAAGCAACCA

Product: phosphoribosylaminoimidazole carboxylase, ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MLHDSYIGPGSTIGIIGGGQLGKMISIAAANLGYKTHLLTNNPDDPSVYITNSATISHNYQNTESLLEFASNVDIATLEF
ENIPTTTIDILSQKIKVYPGKEALYISQNRIREKQHIRNLGIKTSDFRVIDNYNSLVKNTIELGYPTLLKTTELGYDGKG
QYIIKQQDDLSALSTLNWDQSYILEKFVKIYKEISVIITKSISGSIEFFPTAENCHTDGILTTSSVPALISQEINVQAQK
IALQIAESINLVGLLAVEFFITDTQELIVNEIAPRPHNSGHWSLDACNISQFEQLIRAICGLPLKPVKLLFPCIMNNILG
DNIHNYYKHETKVNENLYIYGKKKATKNRKMGHINTLKFNQ

Sequences:

>Translated_361_residues
MLHDSYIGPGSTIGIIGGGQLGKMISIAAANLGYKTHLLTNNPDDPSVYITNSATISHNYQNTESLLEFASNVDIATLEF
ENIPTTTIDILSQKIKVYPGKEALYISQNRIREKQHIRNLGIKTSDFRVIDNYNSLVKNTIELGYPTLLKTTELGYDGKG
QYIIKQQDDLSALSTLNWDQSYILEKFVKIYKEISVIITKSISGSIEFFPTAENCHTDGILTTSSVPALISQEINVQAQK
IALQIAESINLVGLLAVEFFITDTQELIVNEIAPRPHNSGHWSLDACNISQFEQLIRAICGLPLKPVKLLFPCIMNNILG
DNIHNYYKHETKVNENLYIYGKKKATKNRKMGHINTLKFNQ
>Mature_361_residues
MLHDSYIGPGSTIGIIGGGQLGKMISIAAANLGYKTHLLTNNPDDPSVYITNSATISHNYQNTESLLEFASNVDIATLEF
ENIPTTTIDILSQKIKVYPGKEALYISQNRIREKQHIRNLGIKTSDFRVIDNYNSLVKNTIELGYPTLLKTTELGYDGKG
QYIIKQQDDLSALSTLNWDQSYILEKFVKIYKEISVIITKSISGSIEFFPTAENCHTDGILTTSSVPALISQEINVQAQK
IALQIAESINLVGLLAVEFFITDTQELIVNEIAPRPHNSGHWSLDACNISQFEQLIRAICGLPLKPVKLLFPCIMNNILG
DNIHNYYKHETKVNENLYIYGKKKATKNRKMGHINTLKFNQ

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=343, Percent_Identity=27.1137026239067, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788155, Length=367, Percent_Identity=24.2506811989101, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324702, Length=363, Percent_Identity=33.3333333333333, Blast_Score=201, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40395; Mature: 40395

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHDSYIGPGSTIGIIGGGQLGKMISIAAANLGYKTHLLTNNPDDPSVYITNSATISHNY
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCEEEECCC
QNTESLLEFASNVDIATLEFENIPTTTIDILSQKIKVYPGKEALYISQNRIREKQHIRNL
CCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHHHC
GIKTSDFRVIDNYNSLVKNTIELGYPTLLKTTELGYDGKGQYIIKQQDDLSALSTLNWDQ
CCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHCCCCH
SYILEKFVKIYKEISVIITKSISGSIEFFPTAENCHTDGILTTSSVPALISQEINVQAQK
HHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHCCCHHHH
IALQIAESINLVGLLAVEFFITDTQELIVNEIAPRPHNSGHWSLDACNISQFEQLIRAIC
HHHHHHHHCCHHHEEEEHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHH
GLPLKPVKLLFPCIMNNILGDNIHNYYKHETKVNENLYIYGKKKATKNRKMGHINTLKFN
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEEC
Q
C
>Mature Secondary Structure
MLHDSYIGPGSTIGIIGGGQLGKMISIAAANLGYKTHLLTNNPDDPSVYITNSATISHNY
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCEEEECCC
QNTESLLEFASNVDIATLEFENIPTTTIDILSQKIKVYPGKEALYISQNRIREKQHIRNL
CCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHHHC
GIKTSDFRVIDNYNSLVKNTIELGYPTLLKTTELGYDGKGQYIIKQQDDLSALSTLNWDQ
CCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHCCCCH
SYILEKFVKIYKEISVIITKSISGSIEFFPTAENCHTDGILTTSSVPALISQEINVQAQK
HHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHCCCHHHH
IALQIAESINLVGLLAVEFFITDTQELIVNEIAPRPHNSGHWSLDACNISQFEQLIRAIC
HHHHHHHHCCHHHEEEEHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHH
GLPLKPVKLLFPCIMNNILGDNIHNYYKHETKVNENLYIYGKKKATKNRKMGHINTLKFN
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEEC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]