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The map label for this gene is proS

Identifier: 88657672

GI number: 88657672

Start: 749166

End: 750440

Strand: Direct

Name: proS

Synonym: ECH_0740

Alternate gene names: 88657672

Gene position: 749166-750440 (Clockwise)

Preceding gene: 88658438

Following gene: 88658154

Centisome position: 63.69

GC content: 32.08

Gene sequence:

>1275_bases
ATGCGTTTATCAGATTACTATGTACCTACATTAAAAGAAACATCTGCTGATATATCAGTAATATCACATAAATATTCTAT
ACGTGCTGGTCTTATCAAGCAAATTGCCTCCGGCATATACACTTGGCTTCCCTTAGGGTTAAAGGTACTAAAAAATATTG
AAAACATAGTCAGAGAAGAAATGAATAAATCAGGGTCTTTAGAAATATTGATGCCACTGATACAACCAGCAAGCTTATGG
AAAGAATCAGGAAGATACGATGACTATGGATCTGAAATGTTACGCATTACAGATAGAAATCAACGAGAAATGCTTTTTGG
TCCAACCCATGAAGAAGTAATCACTGATATTCTAAGGACAACACCAGTAAGTCATAAAGATCTACCACTAATTCTATACC
AAATACAATGGAAATTTCGCGATGAATTACGGCCAAGATATGGTATAATGAGATGTAGAGAATTCTTAATGAAAGATGCA
TATAGTTTTGATAAAGATTTCAGTGGTGCTATTTCATCTTATAACTTAATGTTCAAAACTTACATCAAAATTTTTCAAAA
GTTAGGCTTAACTCCAATAGCAGTTAAAGCAGATTCAGGACCCATAGGAGGAAATTTAAGTCATGAATTTCATGTATTAG
CAAATTCTGGAGAAAGCACCTTATACTATGACCAAGACATTATTGAATTAATGAATAGTGAGAGTATTGATGTTGAAAAA
ATAAAAAATACTTACACTGCAGCAGATGACATGCATGATCCTCAGGCTTGCCCTATTTCATCAGATAAAGTAAAAATAAG
TAAAGGAATAGAGATAGGTCATATCTTTCATCTAGGAGATAAATATTCAAAACCTATGAATGCTAATTTTTGCGATAGCA
ATAATAATAAGCTCCTACAAATGGGATGTTATGGCATAGGAGTATCAAGGCTAGTAGCAGCAATAATTGAAGTATTTCAT
GATAATAAAGGCATTATTTGGCCAGAAACAGTAGCCCCATTTAAATTTTCCTTAGTAAACTTATATACATCAAATGATAA
ATGTAAGAAAGTTGCAGAAAATCTACACATGCAGTTATATGATGACGTTCTATATGATGACACAGATGATAGTCCTGGTA
TTAAGTTAGCAAGAACAGATCTGCTAGGTATGCCATGGCAAGTTATAATTGGTAAATCAACAGTAGAACAAGACCTTATT
GAAGTAAGGAATAGATTAACAAAAGATAAAGTTTTAATTTCCACAGAACAATTCTTAAATAAATTAAAAAAATGA

Upstream 100 bases:

>100_bases
TTACAAAACAATATTATTTACGTAATAATCGTTTATCACAATTGACTATTGAATATATATATGATATCTGTGCTGCAACT
TGTATTAATTAATATTTTTA

Downstream 100 bases:

>100_bases
TACTAGGGATTGGTACAGACATTGTTTATGTTCCTCGTATATCAAATTTATGGAAAAAATTTGGTACTAAATTTCTTAAA
CGGGTATTTAGTGAAAAAGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MRLSDYYVPTLKETSADISVISHKYSIRAGLIKQIASGIYTWLPLGLKVLKNIENIVREEMNKSGSLEILMPLIQPASLW
KESGRYDDYGSEMLRITDRNQREMLFGPTHEEVITDILRTTPVSHKDLPLILYQIQWKFRDELRPRYGIMRCREFLMKDA
YSFDKDFSGAISSYNLMFKTYIKIFQKLGLTPIAVKADSGPIGGNLSHEFHVLANSGESTLYYDQDIIELMNSESIDVEK
IKNTYTAADDMHDPQACPISSDKVKISKGIEIGHIFHLGDKYSKPMNANFCDSNNNKLLQMGCYGIGVSRLVAAIIEVFH
DNKGIIWPETVAPFKFSLVNLYTSNDKCKKVAENLHMQLYDDVLYDDTDDSPGIKLARTDLLGMPWQVIIGKSTVEQDLI
EVRNRLTKDKVLISTEQFLNKLKK

Sequences:

>Translated_424_residues
MRLSDYYVPTLKETSADISVISHKYSIRAGLIKQIASGIYTWLPLGLKVLKNIENIVREEMNKSGSLEILMPLIQPASLW
KESGRYDDYGSEMLRITDRNQREMLFGPTHEEVITDILRTTPVSHKDLPLILYQIQWKFRDELRPRYGIMRCREFLMKDA
YSFDKDFSGAISSYNLMFKTYIKIFQKLGLTPIAVKADSGPIGGNLSHEFHVLANSGESTLYYDQDIIELMNSESIDVEK
IKNTYTAADDMHDPQACPISSDKVKISKGIEIGHIFHLGDKYSKPMNANFCDSNNNKLLQMGCYGIGVSRLVAAIIEVFH
DNKGIIWPETVAPFKFSLVNLYTSNDKCKKVAENLHMQLYDDVLYDDTDDSPGIKLARTDLLGMPWQVIIGKSTVEQDLI
EVRNRLTKDKVLISTEQFLNKLKK
>Mature_424_residues
MRLSDYYVPTLKETSADISVISHKYSIRAGLIKQIASGIYTWLPLGLKVLKNIENIVREEMNKSGSLEILMPLIQPASLW
KESGRYDDYGSEMLRITDRNQREMLFGPTHEEVITDILRTTPVSHKDLPLILYQIQWKFRDELRPRYGIMRCREFLMKDA
YSFDKDFSGAISSYNLMFKTYIKIFQKLGLTPIAVKADSGPIGGNLSHEFHVLANSGESTLYYDQDIIELMNSESIDVEK
IKNTYTAADDMHDPQACPISSDKVKISKGIEIGHIFHLGDKYSKPMNANFCDSNNNKLLQMGCYGIGVSRLVAAIIEVFH
DNKGIIWPETVAPFKFSLVNLYTSNDKCKKVAENLHMQLYDDVLYDDTDDSPGIKLARTDLLGMPWQVIIGKSTVEQDLI
EVRNRLTKDKVLISTEQFLNKLKK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=428, Percent_Identity=35.7476635514019, Blast_Score=265, Evalue=7e-71,
Organism=Escherichia coli, GI1786392, Length=223, Percent_Identity=57.847533632287, Blast_Score=286, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI115532348, Length=395, Percent_Identity=33.4177215189873, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI193203271, Length=86, Percent_Identity=36.046511627907, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=193, Percent_Identity=40.4145077720207, Blast_Score=163, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24656200, Length=390, Percent_Identity=34.3589743589744, Blast_Score=248, Evalue=6e-66,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_EHRCR (Q2GG92)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507541.1
- ProteinModelPortal:   Q2GG92
- SMR:   Q2GG92
- STRING:   Q2GG92
- GeneID:   3927018
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0740
- TIGR:   ECH_0740
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q2GG92
- ProtClustDB:   PRK12325
- BioCyc:   ECHA205920:ECH_0740-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 48374; Mature: 48374

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSDYYVPTLKETSADISVISHKYSIRAGLIKQIASGIYTWLPLGLKVLKNIENIVREE
CCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKSGSLEILMPLIQPASLWKESGRYDDYGSEMLRITDRNQREMLFGPTHEEVITDILRT
HCCCCCCEEHHHHHCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEECCCHHHHHHHHHHH
TPVSHKDLPLILYQIQWKFRDELRPRYGIMRCREFLMKDAYSFDKDFSGAISSYNLMFKT
CCCCCCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YIKIFQKLGLTPIAVKADSGPIGGNLSHEFHVLANSGESTLYYDQDIIELMNSESIDVEK
HHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCEEEECHHHHHHHCCCCCCHHH
IKNTYTAADDMHDPQACPISSDKVKISKGIEIGHIFHLGDKYSKPMNANFCDSNNNKLLQ
HHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCHHCCCCCCCCCCCCCCEEEE
MGCYGIGVSRLVAAIIEVFHDNKGIIWPETVAPFKFSLVNLYTSNDKCKKVAENLHMQLY
ECCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHH
DDVLYDDTDDSPGIKLARTDLLGMPWQVIIGKSTVEQDLIEVRNRLTKDKVLISTEQFLN
HHHHCCCCCCCCCCEEEEHHHCCCCEEEEECCHHHHHHHHHHHHHHCHHHEEEEHHHHHH
KLKK
HHCC
>Mature Secondary Structure
MRLSDYYVPTLKETSADISVISHKYSIRAGLIKQIASGIYTWLPLGLKVLKNIENIVREE
CCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKSGSLEILMPLIQPASLWKESGRYDDYGSEMLRITDRNQREMLFGPTHEEVITDILRT
HCCCCCCEEHHHHHCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEECCCHHHHHHHHHHH
TPVSHKDLPLILYQIQWKFRDELRPRYGIMRCREFLMKDAYSFDKDFSGAISSYNLMFKT
CCCCCCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YIKIFQKLGLTPIAVKADSGPIGGNLSHEFHVLANSGESTLYYDQDIIELMNSESIDVEK
HHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCEEEECHHHHHHHCCCCCCHHH
IKNTYTAADDMHDPQACPISSDKVKISKGIEIGHIFHLGDKYSKPMNANFCDSNNNKLLQ
HHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCHHCCCCCCCCCCCCCCEEEE
MGCYGIGVSRLVAAIIEVFHDNKGIIWPETVAPFKFSLVNLYTSNDKCKKVAENLHMQLY
ECCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHH
DDVLYDDTDDSPGIKLARTDLLGMPWQVIIGKSTVEQDLIEVRNRLTKDKVLISTEQFLN
HHHHCCCCCCCCCCEEEEHHHCCCCEEEEECCHHHHHHHHHHHHHHCHHHEEEEHHHHHH
KLKK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA