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The map label for this gene is def [H]

Identifier: 88657592

GI number: 88657592

Start: 959364

End: 959948

Strand: Reverse

Name: def [H]

Synonym: ECH_0939

Alternate gene names: 88657592

Gene position: 959948-959364 (Counterclockwise)

Preceding gene: 88657632

Following gene: 88658649

Centisome position: 81.61

GC content: 29.06

Gene sequence:

>585_bases
ATGAATATCTTATCTGTTGATAATGTTCAAGATTTACAAAATTTGCATGCTATATCTCACCCTATTGAAAAGATAGATCA
AGAAATTATAGCGTTAGCTAATGATATGATGAAGGTTATGGAACATAGTAAGACTGTAGGGTTATCTGCAGTTCAACTTG
GTAATCATAGTAGAATGTTTATCATAAATATGTTTAGTGGTTTATTTGATATTGCACAGGATATAAAAGTTTTATCTGGA
CATCATTCATTACATGGTAAAAATATGATATGTATAAATCCTGAAGTTCTCAGCTTCTCTGCTGAAACTGTTGATTTATT
TGAAGGATGTTCATCTGCTAAATCCTATGGATTGATTAATATTACAAGACCAAAACATATGGATTTGAGGTATACAGATT
TGTTAGGGAATGAATGTATAGTGAGGGTCTATGGTTGGCTTTCTAGATGTATACAACATGAACTTGATCACTTAAATGGA
ATATTATTAGCTAATGTTGTAGATAATATAAAAAATAATTGTGTACATAGTATTTCTCAGGAAGATCATAGTGTAGTACA
TATATTACTTGTAAATAAAAAATGA

Upstream 100 bases:

>100_bases
ACTTATATAACTAATAAATGTGTATAACAACTGTTAAGATAATGAAATATATAGAATTATACTGTAAATTGCTTATACTA
AAAGTTGAGAGGAATATAAT

Downstream 100 bases:

>100_bases
TGTTCTTATTTATAGGAAATTTATGTAAGTAGTGCTATATTCTCACAGAAAACAGTGAATTATTTAAAAATGCATTATAC
ATATGATAACGGTGCTAAAT

Product: putative polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MNILSVDNVQDLQNLHAISHPIEKIDQEIIALANDMMKVMEHSKTVGLSAVQLGNHSRMFIINMFSGLFDIAQDIKVLSG
HHSLHGKNMICINPEVLSFSAETVDLFEGCSSAKSYGLINITRPKHMDLRYTDLLGNECIVRVYGWLSRCIQHELDHLNG
ILLANVVDNIKNNCVHSISQEDHSVVHILLVNKK

Sequences:

>Translated_194_residues
MNILSVDNVQDLQNLHAISHPIEKIDQEIIALANDMMKVMEHSKTVGLSAVQLGNHSRMFIINMFSGLFDIAQDIKVLSG
HHSLHGKNMICINPEVLSFSAETVDLFEGCSSAKSYGLINITRPKHMDLRYTDLLGNECIVRVYGWLSRCIQHELDHLNG
ILLANVVDNIKNNCVHSISQEDHSVVHILLVNKK
>Mature_194_residues
MNILSVDNVQDLQNLHAISHPIEKIDQEIIALANDMMKVMEHSKTVGLSAVQLGNHSRMFIINMFSGLFDIAQDIKVLSG
HHSLHGKNMICINPEVLSFSAETVDLFEGCSSAKSYGLINITRPKHMDLRYTDLLGNECIVRVYGWLSRCIQHELDHLNG
ILLANVVDNIKNNCVHSISQEDHSVVHILLVNKK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=177, Percent_Identity=27.683615819209, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21761; Mature: 21761

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILSVDNVQDLQNLHAISHPIEKIDQEIIALANDMMKVMEHSKTVGLSAVQLGNHSRMF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEE
IINMFSGLFDIAQDIKVLSGHHSLHGKNMICINPEVLSFSAETVDLFEGCSSAKSYGLIN
EHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHEECCHHHHHHHHHHHHHCCCCEEE
ITRPKHMDLRYTDLLGNECIVRVYGWLSRCIQHELDHLNGILLANVVDNIKNNCVHSISQ
ECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
EDHSVVHILLVNKK
CCCCEEEEEEEECC
>Mature Secondary Structure
MNILSVDNVQDLQNLHAISHPIEKIDQEIIALANDMMKVMEHSKTVGLSAVQLGNHSRMF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEE
IINMFSGLFDIAQDIKVLSGHHSLHGKNMICINPEVLSFSAETVDLFEGCSSAKSYGLIN
EHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHEECCHHHHHHHHHHHHHCCCCEEE
ITRPKHMDLRYTDLLGNECIVRVYGWLSRCIQHELDHLNGILLANVVDNIKNNCVHSISQ
ECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
EDHSVVHILLVNKK
CCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA