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The map label for this gene is pdxH

Identifier: 88657588

GI number: 88657588

Start: 953473

End: 954072

Strand: Reverse

Name: pdxH

Synonym: ECH_0931

Alternate gene names: 88657588

Gene position: 954072-953473 (Counterclockwise)

Preceding gene: 88658265

Following gene: 88657779

Centisome position: 81.11

GC content: 33.0

Gene sequence:

>600_bases
ATGATTAAGAAAGATCCTATTGAATTGTTTGATTTATGGTATAATGAGGTATTAGCAGTTTCTTTACAAGATAAAAAAGA
TCCTACAGCAATGGTGCTTGCTACATGTAGTAAAGATTTGAAACCTTCAGCAAGGGTTGTTCTGTTAAAAAAGTATAGTG
ATCAAGGTTTTGTATTTTTTACCAATATGAATAGTCGGAAAGGAAAAGAAATGGCAGAAAATCCTTCTGTTGCTCTTGTT
TTTGATTGGAGTCGTATTTCAAAGCAGGTTAGAATTGAAGGGAGAATAAAAATGCTACCTTGTAATGATGCAGATGAGTA
TTATGCATCTAGACCTCGTGGTAGTCAGATAGGTGCTTGGTGTTCTAAGCAGTCAAGTGTTTTAGAAAATAGGGAAGATT
TTGTTGAGTTAATAAAAGAAATGACAATAAAATTCCATGAAAAACCAATACCTAGACCAGATTATTGGGTTGGAATAGTA
GTGGTTCCAATGTTAATGGAATTTTGGCAGGAAGGTTTAAATAGGATTCACACTAGATATCAATATACTAGGGATAGTAA
TAATATGGATAAATGGAATGTTGTATCGCTGTATCCATAA

Upstream 100 bases:

>100_bases
AATATCTTAGATCATTAAATAAGTAAGATAACCTTATTATGTATTAAATAATTAAGTAGAGTGTGAAAATGTCTGTGTTG
GTTGATGTTAAAGGTAGAGT

Downstream 100 bases:

>100_bases
CTGTAGGGTTAATGATGAATATGTTTACAGTAAAACTTAAGTGAAATTTTTATAGTGAATAGAATTTGTTTGAATAATTA
ATACATCTTGGATAATGTTC

Product: pyridoxamine 5'-phosphate oxidase

Products: NA

Alternate protein names: PNP/PMP oxidase; PNPOx; Pyridoxal 5'-phosphate synthase

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIKKDPIELFDLWYNEVLAVSLQDKKDPTAMVLATCSKDLKPSARVVLLKKYSDQGFVFFTNMNSRKGKEMAENPSVALV
FDWSRISKQVRIEGRIKMLPCNDADEYYASRPRGSQIGAWCSKQSSVLENREDFVELIKEMTIKFHEKPIPRPDYWVGIV
VVPMLMEFWQEGLNRIHTRYQYTRDSNNMDKWNVVSLYP

Sequences:

>Translated_199_residues
MIKKDPIELFDLWYNEVLAVSLQDKKDPTAMVLATCSKDLKPSARVVLLKKYSDQGFVFFTNMNSRKGKEMAENPSVALV
FDWSRISKQVRIEGRIKMLPCNDADEYYASRPRGSQIGAWCSKQSSVLENREDFVELIKEMTIKFHEKPIPRPDYWVGIV
VVPMLMEFWQEGLNRIHTRYQYTRDSNNMDKWNVVSLYP
>Mature_199_residues
MIKKDPIELFDLWYNEVLAVSLQDKKDPTAMVLATCSKDLKPSARVVLLKKYSDQGFVFFTNMNSRKGKEMAENPSVALV
FDWSRISKQVRIEGRIKMLPCNDADEYYASRPRGSQIGAWCSKQSSVLENREDFVELIKEMTIKFHEKPIPRPDYWVGIV
VVPMLMEFWQEGLNRIHTRYQYTRDSNNMDKWNVVSLYP

Specific function: Catalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP)

COG id: COG0259

COG function: function code H; Pyridoxamine-phosphate oxidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyridoxamine 5'-phosphate oxidase family

Homologues:

Organism=Homo sapiens, GI8922498, Length=186, Percent_Identity=45.1612903225806, Blast_Score=169, Evalue=1e-42,
Organism=Escherichia coli, GI1787926, Length=195, Percent_Identity=40, Blast_Score=157, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17553712, Length=198, Percent_Identity=39.3939393939394, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319509, Length=204, Percent_Identity=38.7254901960784, Blast_Score=154, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45551845, Length=187, Percent_Identity=37.9679144385027, Blast_Score=123, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24644901, Length=187, Percent_Identity=37.9679144385027, Blast_Score=123, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24644903, Length=172, Percent_Identity=26.1627906976744, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXH_EHRCR (Q2GFR2)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507721.1
- ProteinModelPortal:   Q2GFR2
- SMR:   Q2GFR2
- STRING:   Q2GFR2
- GeneID:   3927709
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0931
- TIGR:   ECH_0931
- eggNOG:   COG0259
- HOGENOM:   HBG327559
- OMA:   FTFFTNY
- PhylomeDB:   Q2GFR2
- ProtClustDB:   CLSK749254
- BioCyc:   ECHA205920:ECH_0931-MONOMER
- HAMAP:   MF_01629
- InterPro:   IPR000659
- InterPro:   IPR019740
- InterPro:   IPR019576
- InterPro:   IPR011576
- InterPro:   IPR012349
- InterPro:   IPR009002
- Gene3D:   G3DSA:2.30.110.10
- PANTHER:   PTHR10851
- PIRSF:   PIRSF000190
- TIGRFAMs:   TIGR00558

Pfam domain/function: PF10590 PNPOx_C; PF01243 Pyridox_oxidase; SSF50475 FMN_binding

EC number: =1.4.3.5

Molecular weight: Translated: 23296; Mature: 23296

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS01064 PYRIDOX_OXIDASE

Important sites: BINDING 45-45 BINDING 48-48 BINDING 50-50 BINDING 67-67 BINDING 107-107 BINDING 111-111 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKDPIELFDLWYNEVLAVSLQDKKDPTAMVLATCSKDLKPSARVVLLKKYSDQGFVFF
CCCCCHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCEEEE
TNMNSRKGKEMAENPSVALVFDWSRISKQVRIEGRIKMLPCNDADEYYASRPRGSQIGAW
ECCCCCCCCHHHCCCCEEEEEEHHHHCCEEEECCEEEEEECCCCHHHHHCCCCCCCHHHH
CSKQSSVLENREDFVELIKEMTIKFHEKPIPRPDYWVGIVVVPMLMEFWQEGLNRIHTRY
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QYTRDSNNMDKWNVVSLYP
EEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MIKKDPIELFDLWYNEVLAVSLQDKKDPTAMVLATCSKDLKPSARVVLLKKYSDQGFVFF
CCCCCHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCEEEE
TNMNSRKGKEMAENPSVALVFDWSRISKQVRIEGRIKMLPCNDADEYYASRPRGSQIGAW
ECCCCCCCCHHHCCCCEEEEEEHHHHCCEEEECCEEEEEECCCCHHHHHCCCCCCCHHHH
CSKQSSVLENREDFVELIKEMTIKFHEKPIPRPDYWVGIVVVPMLMEFWQEGLNRIHTRY
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QYTRDSNNMDKWNVVSLYP
EEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA