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The map label for this gene is gatA

Identifier: 88608215

GI number: 88608215

Start: 217455

End: 218903

Strand: Direct

Name: gatA

Synonym: NSE_0256

Alternate gene names: 88608215

Gene position: 217455-218903 (Clockwise)

Preceding gene: 88607981

Following gene: 88608343

Centisome position: 25.31

GC content: 43.2

Gene sequence:

>1449_bases
GTGAACGATCTAATTAAGTTGAGTGTTGCTAAGATGCATCTTGCATTGGCGCGTCGGGAAATTTCTTCTCTAGAACTAGT
TGAGGCTCATCTATTGCAGATAAGAGAGCGAAATGCCCACACCAATGCGTTGATACTTGTAACAGAGGAAGAAGCTAGAC
AAAGAGCGAAGCTTGCGGATGAGCGCCTCAAAAGAGGGGAGAGTGTAAATACACTTACCGGCATACCTTGCGTCATTAAG
GACATGTATTGCACTAAAGGTGTCAGGACCACTGCTGCATCGCGTGCTTTGAGAAATTTTGTGCCGACGTATGAGTCTAC
GGTAACAAGTAAGCTGATTGATGCTGGTGCAGTGATGCTTGGCAAAGCCAACATGGATGAATTTGCTATGGGTTCGTCTA
ATACTTCTAGTTTCTTTGGTCCAGTAAAGAATCCACTTAAGGGAAAGAAAGGAGTTGATCTTGTTCCTGGTGGTTCGTCT
GGTGGTTCTGCTGCAGCAGTTGCGGACTATTATTCCCCATTCTCTCTTGGCAGTGATACAGGTGGTTCTGTGCGGCAGCC
TGCTTCTTTTTGTGGTCTTGTTGGTCTTAGACCGACTTATGGGCGCTGTTCACGTTGGGGAATGATTCCACTCGCAAATT
CTTTGGACCAGGCCGGTATACTTAGTAGAAATGTTGAGGACAATGCAGCAGTTTTTGAGGTGATTTCTGGTTATGATCGT
AAAGATTCTACATGTGCTAAACTGGAGCCGTTCAAATATAATAAAAACCCAGATATACGTCATATAAAGGTTGGCATACC
CCAGGAGTGTAAGGTTAGTGGTCTGAGCTCACATATAGTGAAATTATGGGAAAGTACAGCTAATATATTGAGGCAACTTG
GGGCAGAAATAATTGATGTCTCGCTTCCATGTGTGGAGTATTCCTTGATGGTGTATTACATCATATGTTCCGCTGAAGCG
TCCTCCCAGTTGGCGCGCTATGATGGTATACGCTACTCTCCTGATCCTTCAATTAGTGCATCTACTATTTCTGAATTATA
CGAGAAATATCGTTCGGTTTCTTTTGGTAGGGAGGTAAAACGCAGGCTGTTTATGGGTACACATGTACTCTCTTCTTCTG
GCTACGCCGATTACTATGCAGCAGCGAAGGAACTGCAAGAGGAGATTGTCAATGAATATTCTGCAGTGTTTGAAAAGGTC
GATGTAATACTCAATCCAACTGCACCGAATGATGCATTTCCAATTGACGGAAAATTAGAACCATTGGAGATGTACATGAA
TGATATTTTCACTGTTCCAACAAGTGTTGCCAAGCTACCATGTATTTCAATTCCTGTAGGGCTGTCCGAAGATGATATGC
CATTGGGTATGCATCTTACGGCAAATTATTTTGCTGAAGAGTTACTCTTGAACGTTGCTCTAGCTTTGCAAAATGCGCTT
TACAAGTAA

Upstream 100 bases:

>100_bases
TGTTTTAATGGTCTAGCTTCTCCTTTTATGGGGTCAACTTGTTTGCAATGCATGTTCTATGGTGGTAAACGTTTTTCCGT
TTTCTAAGGTGTGAGTAGTG

Downstream 100 bases:

>100_bases
GTTGTGCAATGAGGGAATTTAATATTTGGGTGCTATCTTTTATTTTTTGCAGTCAAAAGTTATAATACTGGTCGGTTTGG
GTGCTGTGTTTATGGCGAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNDLIKLSVAKMHLALARREISSLELVEAHLLQIRERNAHTNALILVTEEEARQRAKLADERLKRGESVNTLTGIPCVIK
DMYCTKGVRTTAASRALRNFVPTYESTVTSKLIDAGAVMLGKANMDEFAMGSSNTSSFFGPVKNPLKGKKGVDLVPGGSS
GGSAAAVADYYSPFSLGSDTGGSVRQPASFCGLVGLRPTYGRCSRWGMIPLANSLDQAGILSRNVEDNAAVFEVISGYDR
KDSTCAKLEPFKYNKNPDIRHIKVGIPQECKVSGLSSHIVKLWESTANILRQLGAEIIDVSLPCVEYSLMVYYIICSAEA
SSQLARYDGIRYSPDPSISASTISELYEKYRSVSFGREVKRRLFMGTHVLSSSGYADYYAAAKELQEEIVNEYSAVFEKV
DVILNPTAPNDAFPIDGKLEPLEMYMNDIFTVPTSVAKLPCISIPVGLSEDDMPLGMHLTANYFAEELLLNVALALQNAL
YK

Sequences:

>Translated_482_residues
MNDLIKLSVAKMHLALARREISSLELVEAHLLQIRERNAHTNALILVTEEEARQRAKLADERLKRGESVNTLTGIPCVIK
DMYCTKGVRTTAASRALRNFVPTYESTVTSKLIDAGAVMLGKANMDEFAMGSSNTSSFFGPVKNPLKGKKGVDLVPGGSS
GGSAAAVADYYSPFSLGSDTGGSVRQPASFCGLVGLRPTYGRCSRWGMIPLANSLDQAGILSRNVEDNAAVFEVISGYDR
KDSTCAKLEPFKYNKNPDIRHIKVGIPQECKVSGLSSHIVKLWESTANILRQLGAEIIDVSLPCVEYSLMVYYIICSAEA
SSQLARYDGIRYSPDPSISASTISELYEKYRSVSFGREVKRRLFMGTHVLSSSGYADYYAAAKELQEEIVNEYSAVFEKV
DVILNPTAPNDAFPIDGKLEPLEMYMNDIFTVPTSVAKLPCISIPVGLSEDDMPLGMHLTANYFAEELLLNVALALQNAL
YK
>Mature_482_residues
MNDLIKLSVAKMHLALARREISSLELVEAHLLQIRERNAHTNALILVTEEEARQRAKLADERLKRGESVNTLTGIPCVIK
DMYCTKGVRTTAASRALRNFVPTYESTVTSKLIDAGAVMLGKANMDEFAMGSSNTSSFFGPVKNPLKGKKGVDLVPGGSS
GGSAAAVADYYSPFSLGSDTGGSVRQPASFCGLVGLRPTYGRCSRWGMIPLANSLDQAGILSRNVEDNAAVFEVISGYDR
KDSTCAKLEPFKYNKNPDIRHIKVGIPQECKVSGLSSHIVKLWESTANILRQLGAEIIDVSLPCVEYSLMVYYIICSAEA
SSQLARYDGIRYSPDPSISASTISELYEKYRSVSFGREVKRRLFMGTHVLSSSGYADYYAAAKELQEEIVNEYSAVFEKV
DVILNPTAPNDAFPIDGKLEPLEMYMNDIFTVPTSVAKLPCISIPVGLSEDDMPLGMHLTANYFAEELLLNVALALQNAL
YK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=38.508064516129, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI195972892, Length=502, Percent_Identity=24.9003984063745, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17543272, Length=446, Percent_Identity=36.9955156950673, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=512, Percent_Identity=23.4375, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=303, Percent_Identity=27.0627062706271, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=314, Percent_Identity=26.1146496815287, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=260, Percent_Identity=26.5384615384615, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=260, Percent_Identity=26.5384615384615, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=421, Percent_Identity=36.5795724465558, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=468, Percent_Identity=29.2735042735043, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=203, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=38.9795918367347, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24652985, Length=489, Percent_Identity=24.1308793456033, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922090, Length=489, Percent_Identity=24.1308793456033, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652981, Length=489, Percent_Identity=24.1308793456033, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652983, Length=489, Percent_Identity=24.1308793456033, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644968, Length=509, Percent_Identity=24.5579567779961, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=25.9259259259259, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648435, Length=500, Percent_Identity=24.8, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648437, Length=500, Percent_Identity=24.8, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550774, Length=204, Percent_Identity=31.3725490196078, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356731, Length=515, Percent_Identity=23.3009708737864, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648441, Length=430, Percent_Identity=24.6511627906977, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648439, Length=430, Percent_Identity=24.6511627906977, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_NEOSM (Q2GEE7)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506149.1
- ProteinModelPortal:   Q2GEE7
- SMR:   Q2GEE7
- STRING:   Q2GEE7
- GeneID:   3932049
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0256
- TIGR:   NSE_0256
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q2GEE7
- ProtClustDB:   PRK00012
- BioCyc:   NSEN222891:NSE_0256-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52694; Mature: 52694

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00571 AMIDASES; PS00094 C5_MTASE_1

Important sites: ACT_SITE 80-80 ACT_SITE 159-159 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLIKLSVAKMHLALARREISSLELVEAHLLQIRERNAHTNALILVTEEEARQRAKLAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHH
ERLKRGESVNTLTGIPCVIKDMYCTKGVRTTAASRALRNFVPTYESTVTSKLIDAGAVML
HHHHCCCCCCHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEE
GKANMDEFAMGSSNTSSFFGPVKNPLKGKKGVDLVPGGSSGGSAAAVADYYSPFSLGSDT
ECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHCCCCCCCCCC
GGSVRQPASFCGLVGLRPTYGRCSRWGMIPLANSLDQAGILSRNVEDNAAVFEVISGYDR
CCCCCCCHHHHHCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCC
KDSTCAKLEPFKYNKNPDIRHIKVGIPQECKVSGLSSHIVKLWESTANILRQLGAEIIDV
CCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHCHHHEEC
SLPCVEYSLMVYYIICSAEASSQLARYDGIRYSPDPSISASTISELYEKYRSVSFGREVK
CCCHHHHHHHHHHHEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RRLFMGTHVLSSSGYADYYAAAKELQEEIVNEYSAVFEKVDVILNPTAPNDAFPIDGKLE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCC
PLEMYMNDIFTVPTSVAKLPCISIPVGLSEDDMPLGMHLTANYFAEELLLNVALALQNAL
HHHHHHHHHHHCCHHHHHCCEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
YK
CC
>Mature Secondary Structure
MNDLIKLSVAKMHLALARREISSLELVEAHLLQIRERNAHTNALILVTEEEARQRAKLAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHH
ERLKRGESVNTLTGIPCVIKDMYCTKGVRTTAASRALRNFVPTYESTVTSKLIDAGAVML
HHHHCCCCCCHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEE
GKANMDEFAMGSSNTSSFFGPVKNPLKGKKGVDLVPGGSSGGSAAAVADYYSPFSLGSDT
ECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHCCCCCCCCCC
GGSVRQPASFCGLVGLRPTYGRCSRWGMIPLANSLDQAGILSRNVEDNAAVFEVISGYDR
CCCCCCCHHHHHCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCC
KDSTCAKLEPFKYNKNPDIRHIKVGIPQECKVSGLSSHIVKLWESTANILRQLGAEIIDV
CCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHCHHHEEC
SLPCVEYSLMVYYIICSAEASSQLARYDGIRYSPDPSISASTISELYEKYRSVSFGREVK
CCCHHHHHHHHHHHEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RRLFMGTHVLSSSGYADYYAAAKELQEEIVNEYSAVFEKVDVILNPTAPNDAFPIDGKLE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCC
PLEMYMNDIFTVPTSVAKLPCISIPVGLSEDDMPLGMHLTANYFAEELLLNVALALQNAL
HHHHHHHHHHHCCHHHHHCCEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
YK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA