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The map label for this gene is hisS

Identifier: 88607924

GI number: 88607924

Start: 128190

End: 129452

Strand: Direct

Name: hisS

Synonym: APH_0120

Alternate gene names: 88607924

Gene position: 128190-129452 (Clockwise)

Preceding gene: 88607223

Following gene: 88607395

Centisome position: 8.71

GC content: 44.18

Gene sequence:

>1263_bases
ATGAAAGTAGGTGGATTTCAACCGGTGCGAGGTACAAAGGACTTACTTTCTGAAGAATATTACAAATTATTCCATATACA
AAATGTTGCTCAAGAAATAGGAGAGCGCTTTGGTTTTATGCCTGTGCAAACCCCTATATTTGAATTCCAAGAGGTTTTTT
GCAAAACTCTGGGGGACTCTACAGACGTTATCGGCAAGGAAATGTATACTTTTGCGGATCGTGGTGGGGATGTACTTGCG
TTGCGTCCGGAATTTACTGCTGCAATCGTGCGGTTATTGTTGAGCGAAAAAATGCAGCCACCGGCACGACTATTCACGGC
AGGCCCAGTGTTTCGCTATGAGCGACCGCAGAAATGTCGGCAGAGACAGTTTCACCAAATAAATTATGAATGCTTTGGCG
CAGGTGGAAGCCAAGCTGACGCTGAAATTATCTCTCTAGCATATTGTGTTCTGGAAGTGTTTGGGTTGCACTGTGATGTC
ACGCTAGAAATAAATTCCCTGGGAAGTAGCGAGTGCATGAACGCCTACAGGGCTAGCTTACTTAGTTTTTTTGAGAAGTA
TCGCTCTGAGCTTTCTGAGGATAGCAGGAGAAGGTTGCAGACTAATCCTCTGAGAATTCTAGATTCGAAAGATGCTACTG
ATAAAGAAATACTCAGTACTGCGCCTTCAATCGAGGATTTTTACGATGCTGAGACTAGAGCTTCCTTTGAAGGATTGAAG
GACCATCTCACGAATTTGGGTATACCATATGCAGTGAATCGAAGATTGGTGAGGGGGTTGGACTACTATACCGGCACCGT
TTTTGAGTATAAAACATCGAGCCTGGGGGCACAGGATGCGATAATTGCTGGTGGTAGATACGACAATCTTGTAGCAGCTA
TGGGCGGTGAAAATGTACCCGCAATAGGATTTGCTGGTGGGGTGGAAAGACTTGCGGCATTGATGAGCTATTCTAGGACA
CGAAAGTTCTGTGTATTTATTTTACCGATTTCTGAGGAAGTCGTAAGTCATGCAATGCGTATCACCTATGAGATTAGACG
GACTTTATCCGGCGTCCAAGTGATCTGCGACATAGTGACCAGATTAAAAACGGGCATCAAACGGGCAGATAGACAGAAAG
CTGATATTGCACTGATTCTAGGAGATGAAGAGGTAAACCGTAACGCAGTATCATGCAAGAACATGCTGACAGGAAAACAA
GAAGAAATCAGCATAAGCAACATTACGGAATATCTTAAAGCGATGATGGCAGAGAGACAGTAG

Upstream 100 bases:

>100_bases
TTTGCAGCATTGTACCAATTTGGTAGATAATTTTGCAAAAAAAATGTACACATTTGCTGTCTTCGGGACACCATAAAAAC
AAGTTTAGGGATAGGGGAAT

Downstream 100 bases:

>100_bases
TTCACCATGTACGAACCAGAAAAAGGCCACGGGTATGGTCTTTGGGGAACGCGCGCTATGTGTTGAAGCTACGCCAAAGA
TTTGAGTATGTTCTATAAAA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKVGGFQPVRGTKDLLSEEYYKLFHIQNVAQEIGERFGFMPVQTPIFEFQEVFCKTLGDSTDVIGKEMYTFADRGGDVLA
LRPEFTAAIVRLLLSEKMQPPARLFTAGPVFRYERPQKCRQRQFHQINYECFGAGGSQADAEIISLAYCVLEVFGLHCDV
TLEINSLGSSECMNAYRASLLSFFEKYRSELSEDSRRRLQTNPLRILDSKDATDKEILSTAPSIEDFYDAETRASFEGLK
DHLTNLGIPYAVNRRLVRGLDYYTGTVFEYKTSSLGAQDAIIAGGRYDNLVAAMGGENVPAIGFAGGVERLAALMSYSRT
RKFCVFILPISEEVVSHAMRITYEIRRTLSGVQVICDIVTRLKTGIKRADRQKADIALILGDEEVNRNAVSCKNMLTGKQ
EEISISNITEYLKAMMAERQ

Sequences:

>Translated_420_residues
MKVGGFQPVRGTKDLLSEEYYKLFHIQNVAQEIGERFGFMPVQTPIFEFQEVFCKTLGDSTDVIGKEMYTFADRGGDVLA
LRPEFTAAIVRLLLSEKMQPPARLFTAGPVFRYERPQKCRQRQFHQINYECFGAGGSQADAEIISLAYCVLEVFGLHCDV
TLEINSLGSSECMNAYRASLLSFFEKYRSELSEDSRRRLQTNPLRILDSKDATDKEILSTAPSIEDFYDAETRASFEGLK
DHLTNLGIPYAVNRRLVRGLDYYTGTVFEYKTSSLGAQDAIIAGGRYDNLVAAMGGENVPAIGFAGGVERLAALMSYSRT
RKFCVFILPISEEVVSHAMRITYEIRRTLSGVQVICDIVTRLKTGIKRADRQKADIALILGDEEVNRNAVSCKNMLTGKQ
EEISISNITEYLKAMMAERQ
>Mature_420_residues
MKVGGFQPVRGTKDLLSEEYYKLFHIQNVAQEIGERFGFMPVQTPIFEFQEVFCKTLGDSTDVIGKEMYTFADRGGDVLA
LRPEFTAAIVRLLLSEKMQPPARLFTAGPVFRYERPQKCRQRQFHQINYECFGAGGSQADAEIISLAYCVLEVFGLHCDV
TLEINSLGSSECMNAYRASLLSFFEKYRSELSEDSRRRLQTNPLRILDSKDATDKEILSTAPSIEDFYDAETRASFEGLK
DHLTNLGIPYAVNRRLVRGLDYYTGTVFEYKTSSLGAQDAIIAGGRYDNLVAAMGGENVPAIGFAGGVERLAALMSYSRT
RKFCVFILPISEEVVSHAMRITYEIRRTLSGVQVICDIVTRLKTGIKRADRQKADIALILGDEEVNRNAVSCKNMLTGKQ
EEISISNITEYLKAMMAERQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=420, Percent_Identity=39.2857142857143, Blast_Score=310, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI71993693, Length=459, Percent_Identity=23.3115468409586, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71993686, Length=459, Percent_Identity=23.3115468409586, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_ANAPZ (Q2GLK4)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504747.1
- ProteinModelPortal:   Q2GLK4
- SMR:   Q2GLK4
- STRING:   Q2GLK4
- GeneID:   3930760
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0120
- NMPDR:   fig|212042.5.peg.117
- TIGR:   APH_0120
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q2GLK4
- ProtClustDB:   PRK00037
- BioCyc:   APHA212042:APH_0120-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47160; Mature: 47160

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGGFQPVRGTKDLLSEEYYKLFHIQNVAQEIGERFGFMPVQTPIFEFQEVFCKTLGDS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
TDVIGKEMYTFADRGGDVLALRPEFTAAIVRLLLSEKMQPPARLFTAGPVFRYERPQKCR
HHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHH
QRQFHQINYECFGAGGSQADAEIISLAYCVLEVFGLHCDVTLEINSLGSSECMNAYRASL
HHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
LSFFEKYRSELSEDSRRRLQTNPLRILDSKDATDKEILSTAPSIEDFYDAETRASFEGLK
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCHHHHHCHHHHHHHHHHH
DHLTNLGIPYAVNRRLVRGLDYYTGTVFEYKTSSLGAQDAIIAGGRYDNLVAAMGGENVP
HHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCHHHHHEECCCCCCC
AIGFAGGVERLAALMSYSRTRKFCVFILPISEEVVSHAMRITYEIRRTLSGVQVICDIVT
CCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLKTGIKRADRQKADIALILGDEEVNRNAVSCKNMLTGKQEEISISNITEYLKAMMAERQ
HHHHHHHHHCCCCCCEEEEECCCHHCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVGGFQPVRGTKDLLSEEYYKLFHIQNVAQEIGERFGFMPVQTPIFEFQEVFCKTLGDS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
TDVIGKEMYTFADRGGDVLALRPEFTAAIVRLLLSEKMQPPARLFTAGPVFRYERPQKCR
HHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHH
QRQFHQINYECFGAGGSQADAEIISLAYCVLEVFGLHCDVTLEINSLGSSECMNAYRASL
HHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
LSFFEKYRSELSEDSRRRLQTNPLRILDSKDATDKEILSTAPSIEDFYDAETRASFEGLK
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCHHHHHCHHHHHHHHHHH
DHLTNLGIPYAVNRRLVRGLDYYTGTVFEYKTSSLGAQDAIIAGGRYDNLVAAMGGENVP
HHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCHHHHHEECCCCCCC
AIGFAGGVERLAALMSYSRTRKFCVFILPISEEVVSHAMRITYEIRRTLSGVQVICDIVT
CCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLKTGIKRADRQKADIALILGDEEVNRNAVSCKNMLTGKQEEISISNITEYLKAMMAERQ
HHHHHHHHHCCCCCCEEEEECCCHHCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA