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The map label for this gene is thiC

Identifier: 88607916

GI number: 88607916

Start: 626150

End: 627823

Strand: Reverse

Name: thiC

Synonym: APH_0600

Alternate gene names: 88607916

Gene position: 627823-626150 (Counterclockwise)

Preceding gene: 88606907

Following gene: 88607186

Centisome position: 42.67

GC content: 42.11

Gene sequence:

>1674_bases
ATGCACATGAATATTTCTGAAATTTACCCATCATCTAATAAAATATACATTAATTGCACTCTATATCCGGATGTTAAGGT
CGGAATGCGTCAAATAAATATTAGAAATTCTTCGCAGCGTTTCCTTCTCTACGATACTGCTGGTCCTTATACTGATTGCG
ATATTAAGGTTAATCTGACTGAAGGAATATCTAGCATAAGGCGAGATTGGATAGCTCGCAGGAATGATACCTATCCTATA
CGTAAGGCTAAGACTAATTCTGATGCTCGAGGTGTAAAGGCTCTTTCTGGAGATGATAGAGTAATATTGAAAGGAATAGC
TGGAGGTGCTCCAGTGACTCAACTCTTTTATGCTAAAAAAGGTATCATAACACCCGAGATGGAGTATGTAGCCGTGCGGG
AGAACGCTTTGCTTGAACAGGCTGAAGCTATCCCTGGTCTGTTATTGCCTACTAAGACACGTAAAATAACACCTGAGTTT
GTGCGTGAAGAGGTGGCTTGCGGAAGGGCTGTAATCCCATCAAATATCAATCATCCGGAATCTGAGCCTATGATTATAGG
TAGAAACTTCCTTGTGAAAATAAACGCTAATATAGGAAATTCTGCCGTACTTTCAAATATCGAAGATGAAGTGAAAAAGA
TGGTTTTAGCAGTTACGTATGGAGCTGATACAGTCATGGATCTGTCTACTGGTCATGATATTCACAACATACGAGAATTG
ATAGTGCGCAACAGCCCTGTGCCTATAGGTACTGTGCCTATATATCAAGCTCTAAACAAAGTTAATGGCATAGTTGGAGA
TTTAAGTTTTGAAGTATTCAAAGAAACGATAATAGAGCAGGCCGAGCAGGGGGTGGATTACTTCACCATACACGCGGGTG
TGTTGAAGAGCTACATTGAGCATACCCGTTCACGCACTACAGGAATTGTATCGCGTGGTGGTGCAATAATGGCACAGTGG
TGCTTGCTACACAACAAAGAGAACTTCTTGTACGAGAATTTTGAAGAGATTTGTGAAATAATGCGTAGTTACGATGTAAC
ATTCTCTTTGGGGGATGGTCTTAGACCTGGCTCAATTAATGACGCCAATGACGTAGCGCAGTTTTTAGAGCTTCGCACTT
TGGGAGAATTGGTAAAAATAGCACGTGCGCATGATTGTCAAGTTATAGTAGAAGGTCCTGGCCATGTGCCTATGCATTTG
ATTGAAGAAAATGTAAAAAAGCAACTTGATTTTTGTGATGAGGCGCCATTTTATACACTAGGCCCTCTGACTACGGATAT
AGCTCCTGGCTATGATCACATAACCAGTGCCATTGGTGCTGCTATGATAGGTTGGTATGGAACTGCGATGTTGTGCTACG
TAACACCTAAAGAGCATCTCGGATTACCCAATGCCGAAGACGTAAAAGCGGGTGTTATATCTTATAAAATCGCGGCTCAC
GCTGCTGACCTGGCGAAAGGTAATCCGGCCTCGTTTGCCCGTGATTATGCTCTCAGCCAAGCAAGATATGACTTTAGGTG
GGAGGACCAGTTTAACTTGTCTATAGATCCCGCAACTGCTAAAAAGCTGCACGATGAATCTTTACCTGGAAAGGGCGGAA
AAACAGCGCATTTTTGTTCCATGTGTGGGCCAAAATTTTGTTCCATGAAACTTAGCAAGGCTCTCAATGATTGA

Upstream 100 bases:

>100_bases
ACATCTTCTGACATAATATGTTTATAAAGAAGAGATTTTTATTTAAGATTGTATAATATATTCCATAAATGTATGCTAAC
CGGGAATATTTTTCATCCTA

Downstream 100 bases:

>100_bases
CGTATTATTTCCGTTAATAGCATTTTTTGTCCATACGCTACAGCGCGCGTTTACAGCTTGAAATGGAACTTTTCGCAGTA
TATTCCCAAGGAAATGAGTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLTEGISSIRRDWIARRNDTYPI
RKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKKGIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEF
VREEVACGRAVIPSNINHPESEPMIIGRNFLVKINANIGNSAVLSNIEDEVKKMVLAVTYGADTVMDLSTGHDIHNIREL
IVRNSPVPIGTVPIYQALNKVNGIVGDLSFEVFKETIIEQAEQGVDYFTIHAGVLKSYIEHTRSRTTGIVSRGGAIMAQW
CLLHNKENFLYENFEEICEIMRSYDVTFSLGDGLRPGSINDANDVAQFLELRTLGELVKIARAHDCQVIVEGPGHVPMHL
IEENVKKQLDFCDEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNAEDVKAGVISYKIAAH
AADLAKGNPASFARDYALSQARYDFRWEDQFNLSIDPATAKKLHDESLPGKGGKTAHFCSMCGPKFCSMKLSKALND

Sequences:

>Translated_557_residues
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLTEGISSIRRDWIARRNDTYPI
RKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKKGIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEF
VREEVACGRAVIPSNINHPESEPMIIGRNFLVKINANIGNSAVLSNIEDEVKKMVLAVTYGADTVMDLSTGHDIHNIREL
IVRNSPVPIGTVPIYQALNKVNGIVGDLSFEVFKETIIEQAEQGVDYFTIHAGVLKSYIEHTRSRTTGIVSRGGAIMAQW
CLLHNKENFLYENFEEICEIMRSYDVTFSLGDGLRPGSINDANDVAQFLELRTLGELVKIARAHDCQVIVEGPGHVPMHL
IEENVKKQLDFCDEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNAEDVKAGVISYKIAAH
AADLAKGNPASFARDYALSQARYDFRWEDQFNLSIDPATAKKLHDESLPGKGGKTAHFCSMCGPKFCSMKLSKALND
>Mature_557_residues
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLTEGISSIRRDWIARRNDTYPI
RKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKKGIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEF
VREEVACGRAVIPSNINHPESEPMIIGRNFLVKINANIGNSAVLSNIEDEVKKMVLAVTYGADTVMDLSTGHDIHNIREL
IVRNSPVPIGTVPIYQALNKVNGIVGDLSFEVFKETIIEQAEQGVDYFTIHAGVLKSYIEHTRSRTTGIVSRGGAIMAQW
CLLHNKENFLYENFEEICEIMRSYDVTFSLGDGLRPGSINDANDVAQFLELRTLGELVKIARAHDCQVIVEGPGHVPMHL
IEENVKKQLDFCDEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNAEDVKAGVISYKIAAH
AADLAKGNPASFARDYALSQARYDFRWEDQFNLSIDPATAKKLHDESLPGKGGKTAHFCSMCGPKFCSMKLSKALND

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=575, Percent_Identity=60.5217391304348, Blast_Score=716, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_ANAPZ (Q2GKB1)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505190.1
- STRING:   Q2GKB1
- GeneID:   3931309
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0600
- NMPDR:   fig|212042.5.peg.589
- TIGR:   APH_0600
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q2GKB1
- ProtClustDB:   PRK09284
- BioCyc:   APHA212042:APH_0600-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 61562; Mature: 61562

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: BINDING 197-197 BINDING 226-226 BINDING 255-255 BINDING 291-291 BINDING 391-391 BINDING 418-418

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLT
CCCCHHHHCCCCCEEEEEEEECCCHHCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEHH
EGISSIRRDWIARRNDTYPIRKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKK
HHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHC
GIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEFVREEVACGRAVIPSNINHPE
CCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCC
SEPMIIGRNFLVKINANIGNSAVLSNIEDEVKKMVLAVTYGADTVMDLSTGHDIHNIREL
CCCEEEECEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCCHHHHHHH
IVRNSPVPIGTVPIYQALNKVNGIVGDLSFEVFKETIIEQAEQGVDYFTIHAGVLKSYIE
HHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
HTRSRTTGIVSRGGAIMAQWCLLHNKENFLYENFEEICEIMRSYDVTFSLGDGLRPGSIN
HHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCHHHHHHHHHHCCCEEEECCCCCCCCCC
DANDVAQFLELRTLGELVKIARAHDCQVIVEGPGHVPMHLIEENVKKQLDFCDEAPFYTL
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEE
GPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNAEDVKAGVISYKIAAH
CCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHH
AADLAKGNPASFARDYALSQARYDFRWEDQFNLSIDPATAKKLHDESLPGKGGKTAHFCS
HHHHCCCCCHHHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHHCCCCCCCCCCHHHHH
MCGPKFCSMKLSKALND
HCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLT
CCCCHHHHCCCCCEEEEEEEECCCHHCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEHH
EGISSIRRDWIARRNDTYPIRKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKK
HHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHC
GIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEFVREEVACGRAVIPSNINHPE
CCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCC
SEPMIIGRNFLVKINANIGNSAVLSNIEDEVKKMVLAVTYGADTVMDLSTGHDIHNIREL
CCCEEEECEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCCHHHHHHH
IVRNSPVPIGTVPIYQALNKVNGIVGDLSFEVFKETIIEQAEQGVDYFTIHAGVLKSYIE
HHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
HTRSRTTGIVSRGGAIMAQWCLLHNKENFLYENFEEICEIMRSYDVTFSLGDGLRPGSIN
HHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCHHHHHHHHHHCCCEEEECCCCCCCCCC
DANDVAQFLELRTLGELVKIARAHDCQVIVEGPGHVPMHLIEENVKKQLDFCDEAPFYTL
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEE
GPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNAEDVKAGVISYKIAAH
CCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHH
AADLAKGNPASFARDYALSQARYDFRWEDQFNLSIDPATAKKLHDESLPGKGGKTAHFCS
HHHHCCCCCHHHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHHCCCCCCCCCCHHHHH
MCGPKFCSMKLSKALND
HCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA