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The map label for this gene is gshB [H]

Identifier: 88607889

GI number: 88607889

Start: 192161

End: 193078

Strand: Direct

Name: gshB [H]

Synonym: APH_0193

Alternate gene names: 88607889

Gene position: 192161-193078 (Clockwise)

Preceding gene: 88606696

Following gene: 88607138

Centisome position: 13.06

GC content: 43.79

Gene sequence:

>918_bases
TTGTTAAAGGTTGCATTTCAGATGGATGCCGATGTAGTTGTCGGCAAAGATCTAACTGTGCAATTTATACAAGAGAGCAA
GCGTAGGGGGCACGATGTGTTTTTCTACCAGCCGCGTGATCTCTCATTCTCGAGAGGTGAGGTTGTTGCTACAGCCTACT
CTGTGGATATAGGAGAGAACTCTTTGCACCTGCGAGACAGGGTGCGGTTGTCGCTGGAGGGGTTGGATATACTGTTTATA
AGGCAGAACCCACCGTTTGATATGCGCTATATTACTAATACATATCTGTTGGAAAGATTAGCTGGGGTACTTTTTGTAAA
TGACCCAAAAGCTATCAGGGACTTTCCTGAGAAATTGCTGCCACTATCGTTTCCTGAATTCATACCGCCTACGCTGATTA
CCGAAAGTGTTCAGGAGGCATGTGAGTTTTACCGGGAATATGGTGATGTAGTAATCAAGCCCTTGTATAGCTTTGGGGGA
GATGGAGTAATCAGGATATGTGGTAATGTCGATATTGCGGTAATATTTGACGTTATGGTGGCCAGGTACGGTACACAGGT
TGTGATACAGCAAATTATACCATCTGTAGAAGATGATAAGAGGGTTGTATTGCTGAATGGCGAGCCTATAGGTGCGATAA
AGCGTAGAGTGATTTCTTCCGGGGAGATAAGGACTAATCTGAGAGTAGGTGCTGTTCCAGAAATGACAGAGTTGTCTGAG
CGTGATAGAGAAATATGTCGCGTGGTAGGAGATAAGTTAACAGCAGCAGGGATTATATTTGCAGGAATAGATATTCTTGG
AGGGTACCTGCTAGAGGTAAATGTAACCTCTCCCTGTGGAATATTAGAGATAAATCAGGTATATGGAACAACCCTTGAAA
AGGAATGTTGGGACCAATTTGAGGGTATGTTGGCTTGA

Upstream 100 bases:

>100_bases
TAATGCCGTGGTGGCGATAGCAAAGCGTGTCCGTGGGGTTAAGCAAGTTATAAGCTACGTGAGATTGAAGTACAGCAGAT
TGCGTCACTAGGGGTCGGTG

Downstream 100 bases:

>100_bases
AGGCGGTTCAATGCTGATAATGTAGACATTGTCTACGGGGGTCGCCTGATGAGGGATGAGCACGGGTATATATCGGCTGA
CGTGTCTTTACGTACGTTTT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MLKVAFQMDADVVVGKDLTVQFIQESKRRGHDVFFYQPRDLSFSRGEVVATAYSVDIGENSLHLRDRVRLSLEGLDILFI
RQNPPFDMRYITNTYLLERLAGVLFVNDPKAIRDFPEKLLPLSFPEFIPPTLITESVQEACEFYREYGDVVIKPLYSFGG
DGVIRICGNVDIAVIFDVMVARYGTQVVIQQIIPSVEDDKRVVLLNGEPIGAIKRRVISSGEIRTNLRVGAVPEMTELSE
RDREICRVVGDKLTAAGIIFAGIDILGGYLLEVNVTSPCGILEINQVYGTTLEKECWDQFEGMLA

Sequences:

>Translated_305_residues
MLKVAFQMDADVVVGKDLTVQFIQESKRRGHDVFFYQPRDLSFSRGEVVATAYSVDIGENSLHLRDRVRLSLEGLDILFI
RQNPPFDMRYITNTYLLERLAGVLFVNDPKAIRDFPEKLLPLSFPEFIPPTLITESVQEACEFYREYGDVVIKPLYSFGG
DGVIRICGNVDIAVIFDVMVARYGTQVVIQQIIPSVEDDKRVVLLNGEPIGAIKRRVISSGEIRTNLRVGAVPEMTELSE
RDREICRVVGDKLTAAGIIFAGIDILGGYLLEVNVTSPCGILEINQVYGTTLEKECWDQFEGMLA
>Mature_305_residues
MLKVAFQMDADVVVGKDLTVQFIQESKRRGHDVFFYQPRDLSFSRGEVVATAYSVDIGENSLHLRDRVRLSLEGLDILFI
RQNPPFDMRYITNTYLLERLAGVLFVNDPKAIRDFPEKLLPLSFPEFIPPTLITESVQEACEFYREYGDVVIKPLYSFGG
DGVIRICGNVDIAVIFDVMVARYGTQVVIQQIIPSVEDDKRVVLLNGEPIGAIKRRVISSGEIRTNLRVGAVPEMTELSE
RDREICRVVGDKLTAAGIIFAGIDILGGYLLEVNVTSPCGILEINQVYGTTLEKECWDQFEGMLA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=313, Percent_Identity=33.2268370607029, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34246; Mature: 34246

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVAFQMDADVVVGKDLTVQFIQESKRRGHDVFFYQPRDLSFSRGEVVATAYSVDIGEN
CEEEEEECCCCEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEEECCCC
SLHLRDRVRLSLEGLDILFIRQNPPFDMRYITNTYLLERLAGVLFVNDPKAIRDFPEKLL
CEEEHHHEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHC
PLSFPEFIPPTLITESVQEACEFYREYGDVVIKPLYSFGGDGVIRICGNVDIAVIFDVMV
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCEEEEECCCCHHHHHHHHH
ARYGTQVVIQQIIPSVEDDKRVVLLNGEPIGAIKRRVISSGEIRTNLRVGAVPEMTELSE
HHHCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEECEEECCCCCHHHHHH
RDREICRVVGDKLTAAGIIFAGIDILGGYLLEVNVTSPCGILEINQVYGTTLEKECWDQF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHHCCCHHHHHHHHH
EGMLA
HCCCC
>Mature Secondary Structure
MLKVAFQMDADVVVGKDLTVQFIQESKRRGHDVFFYQPRDLSFSRGEVVATAYSVDIGEN
CEEEEEECCCCEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEEECCCC
SLHLRDRVRLSLEGLDILFIRQNPPFDMRYITNTYLLERLAGVLFVNDPKAIRDFPEKLL
CEEEHHHEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHC
PLSFPEFIPPTLITESVQEACEFYREYGDVVIKPLYSFGGDGVIRICGNVDIAVIFDVMV
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCEEEEECCCCHHHHHHHHH
ARYGTQVVIQQIIPSVEDDKRVVLLNGEPIGAIKRRVISSGEIRTNLRVGAVPEMTELSE
HHHCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEECEEECCCCCHHHHHH
RDREICRVVGDKLTAAGIIFAGIDILGGYLLEVNVTSPCGILEINQVYGTTLEKECWDQF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHHCCCHHHHHHHHH
EGMLA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1540152 [H]