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The map label for this gene is rnhA [H]

Identifier: 88607880

GI number: 88607880

Start: 114020

End: 114472

Strand: Direct

Name: rnhA [H]

Synonym: APH_0109

Alternate gene names: 88607880

Gene position: 114020-114472 (Clockwise)

Preceding gene: 88607343

Following gene: 88606992

Centisome position: 7.75

GC content: 46.8

Gene sequence:

>453_bases
ATGGATAGTGGGCAGGTTGTCATATACACTGATGGAGCTTGTTCTGGTAATCCTGGCCCTGGCGGCTGGGGTGCGGTCTT
TCTTTTTTCGGGTGGTGAGGAGCGTAGGATATCAGGTGGTTGTAGCGATACAACAAACAACAGGATGGAGCTTTCTGCAG
TGATAGAAGCTTTAGGTGCTTTAGATAGGCAATGTAACGTTTGTGTGCGTACGGATAGTGTCTATGTGAAGAATGGTATT
ACTACGTGGATACACAAGTGGAAGTCAAATGGCTGGCAAACTGCGAGTAAGTCTGCTGTAAAAAATGTAGATTTGTGGAT
GCAGCTTGAGGCACTGACGCTGAAGCATAATGTGACGTGGGAATGGGTTAAGGCTCATGCAGGTAACAGGTATAATGTTG
AGGCTGATGCACTGGCAAGAGCTGAAGTAGAGCGCCAAGTTATGAAGCGTTGA

Upstream 100 bases:

>100_bases
TGAATAGCCTCTCTACGCAAGGCTGTAGGTACCAACACGAATGTCATTGTATTATGCAGGGTATTGGAATACAATCAAAA
ACGGTGGATTTGTGGTACTT

Downstream 100 bases:

>100_bases
GGGGGAACTTTGCCAGCGTTGAGGTGTTGATTAGATTGATGAATTCTATAGCGTGGATTTGCATAAAGTTTTTGTTTATA
GGTGCAGTACTGCTGTTTGG

Product: ribonuclease H1

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MDSGQVVIYTDGACSGNPGPGGWGAVFLFSGGEERRISGGCSDTTNNRMELSAVIEALGALDRQCNVCVRTDSVYVKNGI
TTWIHKWKSNGWQTASKSAVKNVDLWMQLEALTLKHNVTWEWVKAHAGNRYNVEADALARAEVERQVMKR

Sequences:

>Translated_150_residues
MDSGQVVIYTDGACSGNPGPGGWGAVFLFSGGEERRISGGCSDTTNNRMELSAVIEALGALDRQCNVCVRTDSVYVKNGI
TTWIHKWKSNGWQTASKSAVKNVDLWMQLEALTLKHNVTWEWVKAHAGNRYNVEADALARAEVERQVMKR
>Mature_150_residues
MDSGQVVIYTDGACSGNPGPGGWGAVFLFSGGEERRISGGCSDTTNNRMELSAVIEALGALDRQCNVCVRTDSVYVKNGI
TTWIHKWKSNGWQTASKSAVKNVDLWMQLEALTLKHNVTWEWVKAHAGNRYNVEADALARAEVERQVMKR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=144, Percent_Identity=37.5, Blast_Score=78, Evalue=4e-15,
Organism=Escherichia coli, GI1786408, Length=137, Percent_Identity=56.9343065693431, Blast_Score=162, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI115532542, Length=139, Percent_Identity=38.8489208633094, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17505482, Length=134, Percent_Identity=35.0746268656716, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=36.734693877551, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17137476, Length=147, Percent_Identity=36.734693877551, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16532; Mature: 16532

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGQVVIYTDGACSGNPGPGGWGAVFLFSGGEERRISGGCSDTTNNRMELSAVIEALGA
CCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHH
LDRQCNVCVRTDSVYVKNGITTWIHKWKSNGWQTASKSAVKNVDLWMQLEALTLKHNVTW
CCCCCCEEEECCCEEEECCHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEEEEEEECCCCE
EWVKAHAGNRYNVEADALARAEVERQVMKR
EEEEECCCCEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSGQVVIYTDGACSGNPGPGGWGAVFLFSGGEERRISGGCSDTTNNRMELSAVIEALGA
CCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHH
LDRQCNVCVRTDSVYVKNGITTWIHKWKSNGWQTASKSAVKNVDLWMQLEALTLKHNVTW
CCCCCCEEEECCCEEEECCHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEEEEEEECCCCE
EWVKAHAGNRYNVEADALARAEVERQVMKR
EEEEECCCCEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA