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The map label for this gene is rpiB [H]

Identifier: 88607843

GI number: 88607843

Start: 666581

End: 667012

Strand: Reverse

Name: rpiB [H]

Synonym: APH_0642

Alternate gene names: 88607843

Gene position: 667012-666581 (Counterclockwise)

Preceding gene: 88607230

Following gene: 88607813

Centisome position: 45.34

GC content: 42.59

Gene sequence:

>432_bases
GTGGTAAAAAGGGTTTTTCTCTCCTCTGATCATGCTGGCGTAGAACTCAGATTGTTTCTTAGCGCGTATCTAAGAGACTT
AGGTTGCGAAGTTTTTGACTGCGGTTGCGATCCTAAAGAGCATTCCGTAGACTACCCAGATTACGTACATGACGTTGTAA
GAGAAGTATCAGATACGAGTTTTGGAGTTTTGATATGCGGAACGGGTATAGGCATGAGTATAGCAGCCAACCGTCATAAA
AATATTAGAGCCGCTCTTTGTTCTTCTACCATGCTTGCAAAGCTTTCTAGAGAACACAATGATGCGAATGTTCTGTGTTT
TGGCTCTCGTTATATAGATCCTGATACTGCACAGTCCGTATTATACACGTTCATGACAACGGCGTTCTTAGGGGGAAGAC
ATGCCGTAAGAGTACAAAAACTTGGTGAATGA

Upstream 100 bases:

>100_bases
CTATAAGCTATTTTGGCTTTATATTATTTGAACGCTCCATCAGGAGTTGTAATATGTGTTTTTCAGTAAGGTTATATTAT
CAATAGATAATAAAGTGTTA

Downstream 100 bases:

>100_bases
TACGTATGAAAAAACTGCGTTATTAGAAGATCTTTCGTGTGTTAATCAAGGTCTGCATGGTGTTGCATATCACGCAGCTT
TCCTTTTTCATCAGACCTTA

Product: ribose 5-phosphate isomerase B

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MVKRVFLSSDHAGVELRLFLSAYLRDLGCEVFDCGCDPKEHSVDYPDYVHDVVREVSDTSFGVLICGTGIGMSIAANRHK
NIRAALCSSTMLAKLSREHNDANVLCFGSRYIDPDTAQSVLYTFMTTAFLGGRHAVRVQKLGE

Sequences:

>Translated_143_residues
MVKRVFLSSDHAGVELRLFLSAYLRDLGCEVFDCGCDPKEHSVDYPDYVHDVVREVSDTSFGVLICGTGIGMSIAANRHK
NIRAALCSSTMLAKLSREHNDANVLCFGSRYIDPDTAQSVLYTFMTTAFLGGRHAVRVQKLGE
>Mature_143_residues
MVKRVFLSSDHAGVELRLFLSAYLRDLGCEVFDCGCDPKEHSVDYPDYVHDVVREVSDTSFGVLICGTGIGMSIAANRHK
NIRAALCSSTMLAKLSREHNDANVLCFGSRYIDPDTAQSVLYTFMTTAFLGGRHAVRVQKLGE

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=142, Percent_Identity=40.8450704225352, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 15778; Mature: 15778

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKRVFLSSDHAGVELRLFLSAYLRDLGCEVFDCGCDPKEHSVDYPDYVHDVVREVSDTS
CCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCC
FGVLICGTGIGMSIAANRHKNIRAALCSSTMLAKLSREHNDANVLCFGSRYIDPDTAQSV
CEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHH
LYTFMTTAFLGGRHAVRVQKLGE
HHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MVKRVFLSSDHAGVELRLFLSAYLRDLGCEVFDCGCDPKEHSVDYPDYVHDVVREVSDTS
CCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCC
FGVLICGTGIGMSIAANRHKNIRAALCSSTMLAKLSREHNDANVLCFGSRYIDPDTAQSV
CEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHH
LYTFMTTAFLGGRHAVRVQKLGE
HHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]