Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 88607833

GI number: 88607833

Start: 321322

End: 321897

Strand: Direct

Name: priA [H]

Synonym: APH_0337

Alternate gene names: 88607833

Gene position: 321322-321897 (Clockwise)

Preceding gene: 88607340

Following gene: 88607311

Centisome position: 21.84

GC content: 42.88

Gene sequence:

>576_bases
ATGATATACAATGCGCGAGACATGGCCATTGTATTGGGAAAGCACGTTGATATTCCAGTAGTGTTGTGTACAGCGACTCC
ATCACTGGAAACCATGAATAACATACAGCAAAAGAAGTACAATCATGTAGTATTGAAGAGAAGGTTCGGAGAAGCGGTGA
TGCCAGATATAATAGTGGCGGATATGCGCAAAGACGAACTAAAAAAGGCATGGATGTCTACATGCTTATACGAAAAGATA
TGTGAAACACTTGCAAAACAGCAGCAAGTAATGTTGTTCTTGAACCGACGGGGATATGCAAGGCTTGTACTATGCAAACA
ATGCGGACACAAAGTAAACTGTCCAAACTGTTGTACGTGGCTTACGGAGCACAGAGTTTTAGGGGCAATGCTATGCCACT
ACTGTGCGTATAGTTGCGAGATACCAAGAGAGTGTCCATCGTGCCAAGAGTATAACACCATGAGCCCATATGGTGTGGGT
ATGGAAAGGATACTAGAGGGTATACAAGAACTCATCGATGCAGAACATTTCACTATTCTGAGCAGTGATATAGGTATACC
GGCGAATTCACAATGA

Upstream 100 bases:

>100_bases
GTACTACAAATGGAGCGCGGGAGAAAGAATAGGCACCTTGTCGTTTATCTATATCTTTTTTACTTAGTTTTGCTAGACCT
CAGGAAGGCGTAATGGCGGC

Downstream 100 bases:

>100_bases
TGAGAAACTTAGCCACGATCCCAAAGATATGTCACTCTCTCTTCCAAATTAACCCACACCATAGAAAACACAGATAGTGT
ATCTAGTTAGCGATTGCGCT

Product: primosomal protein N truncation

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MIYNARDMAIVLGKHVDIPVVLCTATPSLETMNNIQQKKYNHVVLKRRFGEAVMPDIIVADMRKDELKKAWMSTCLYEKI
CETLAKQQQVMLFLNRRGYARLVLCKQCGHKVNCPNCCTWLTEHRVLGAMLCHYCAYSCEIPRECPSCQEYNTMSPYGVG
MERILEGIQELIDAEHFTILSSDIGIPANSQ

Sequences:

>Translated_191_residues
MIYNARDMAIVLGKHVDIPVVLCTATPSLETMNNIQQKKYNHVVLKRRFGEAVMPDIIVADMRKDELKKAWMSTCLYEKI
CETLAKQQQVMLFLNRRGYARLVLCKQCGHKVNCPNCCTWLTEHRVLGAMLCHYCAYSCEIPRECPSCQEYNTMSPYGVG
MERILEGIQELIDAEHFTILSSDIGIPANSQ
>Mature_191_residues
MIYNARDMAIVLGKHVDIPVVLCTATPSLETMNNIQQKKYNHVVLKRRFGEAVMPDIIVADMRKDELKKAWMSTCLYEKI
CETLAKQQQVMLFLNRRGYARLVLCKQCGHKVNCPNCCTWLTEHRVLGAMLCHYCAYSCEIPRECPSCQEYNTMSPYGVG
MERILEGIQELIDAEHFTILSSDIGIPANSQ

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=169, Percent_Identity=37.2781065088757, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 21796; Mature: 21796

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
12.0 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
12.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYNARDMAIVLGKHVDIPVVLCTATPSLETMNNIQQKKYNHVVLKRRFGEAVMPDIIVA
CCCCCCHHHHEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHH
DMRKDELKKAWMSTCLYEKICETLAKQQQVMLFLNRRGYARLVLCKQCGHKVNCPNCCTW
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHH
LTEHRVLGAMLCHYCAYSCEIPRECPSCQEYNTMSPYGVGMERILEGIQELIDAEHFTIL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEE
SSDIGIPANSQ
ECCCCCCCCCC
>Mature Secondary Structure
MIYNARDMAIVLGKHVDIPVVLCTATPSLETMNNIQQKKYNHVVLKRRFGEAVMPDIIVA
CCCCCCHHHHEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHH
DMRKDELKKAWMSTCLYEKICETLAKQQQVMLFLNRRGYARLVLCKQCGHKVNCPNCCTW
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHH
LTEHRVLGAMLCHYCAYSCEIPRECPSCQEYNTMSPYGVGMERILEGIQELIDAEHFTIL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEE
SSDIGIPANSQ
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]