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The map label for this gene is yjfH [C]

Identifier: 88607795

GI number: 88607795

Start: 272556

End: 273332

Strand: Reverse

Name: yjfH [C]

Synonym: APH_0275

Alternate gene names: 88607795

Gene position: 273332-272556 (Counterclockwise)

Preceding gene: 88607182

Following gene: 88607047

Centisome position: 18.58

GC content: 39.77

Gene sequence:

>777_bases
ATGAATCGCATTAAAGACAACAGATATCTATGGATTTATGGAAAACATGCTTGTCTTTCTGCCCTAAAAAATCCTAATAG
GGTGTGCATTGAATTACTGATTACTCATAAGTTCATAGATAAAAATGATCCTGTGATTAAGTTAGCTAAAAATAGGAAGA
TAGGAATACGCGATGTAGAAATCGCAAAGATTGATTCTTTAGTGGGAAGTAAAGCCATACATCAAGGCATGTTGCTAAAA
GTGCTCCCGCTATTTGGTCGCAACGCCAGCTCTTTAATTCGCATAGAAGATATAGTTGCACAGACAGAGGCTGCTCCTAC
CTCTACTATACTCGTTCTTGATGAAGTAACTGATGTACATAACGTAGGCGCAGTATTGCGGACAGCAGCTTGCTTTAATG
TAGATGCTGTTGTGTTAACAGAGCATAATTCTCCTTCTGAAAGCTGTGGCATGGCAAAAGCTTCTAGTGGAGCCATAGAC
ATCGTTCCCGTAGTTTGTATTGGCAATCTAGTAAAGACACTGGAATACCTAAAACAAAAAAATTACTGGTGTTACGGGTT
TGATGCTTCAGGAGGGACATGCTTACATCAAGTAGAATTTAGTGAGCGAAGAGTGGTGATTTTAGGTGCAGAAGGCAAGG
GAATGCGCCACCTTACTAAAAAACACTGCGATCACTTGGTTAAAATACCTATGTCAGAGAAGATGGAGAGTCTCAATGTC
TCAAATGCAGCTGCAATAGCGATGTATTCTGTGTTTTTGGGAGACAAGAGATCTTAA

Upstream 100 bases:

>100_bases
AAAACCACTCAAGTTTCCCGAGTTGCCTTAAAAAAATTTAGCCCTACAATACATCTGCATAAATTAGCAAAAATTACCGG
CTTTACAACAATAAAAAACT

Downstream 100 bases:

>100_bases
ATAGCCTTTGCTGGATCACTTACTCGCAGTCAGTAAAGTAAGTTGTCTTGCTTAATTCCTTTAATAATTAAGAATGTCTT
ACTCTGCTCTTCCAGGTGCT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNRIKDNRYLWIYGKHACLSALKNPNRVCIELLITHKFIDKNDPVIKLAKNRKIGIRDVEIAKIDSLVGSKAIHQGMLLK
VLPLFGRNASSLIRIEDIVAQTEAAPTSTILVLDEVTDVHNVGAVLRTAACFNVDAVVLTEHNSPSESCGMAKASSGAID
IVPVVCIGNLVKTLEYLKQKNYWCYGFDASGGTCLHQVEFSERRVVILGAEGKGMRHLTKKHCDHLVKIPMSEKMESLNV
SNAAAIAMYSVFLGDKRS

Sequences:

>Translated_258_residues
MNRIKDNRYLWIYGKHACLSALKNPNRVCIELLITHKFIDKNDPVIKLAKNRKIGIRDVEIAKIDSLVGSKAIHQGMLLK
VLPLFGRNASSLIRIEDIVAQTEAAPTSTILVLDEVTDVHNVGAVLRTAACFNVDAVVLTEHNSPSESCGMAKASSGAID
IVPVVCIGNLVKTLEYLKQKNYWCYGFDASGGTCLHQVEFSERRVVILGAEGKGMRHLTKKHCDHLVKIPMSEKMESLNV
SNAAAIAMYSVFLGDKRS
>Mature_258_residues
MNRIKDNRYLWIYGKHACLSALKNPNRVCIELLITHKFIDKNDPVIKLAKNRKIGIRDVEIAKIDSLVGSKAIHQGMLLK
VLPLFGRNASSLIRIEDIVAQTEAAPTSTILVLDEVTDVHNVGAVLRTAACFNVDAVVLTEHNSPSESCGMAKASSGAID
IVPVVCIGNLVKTLEYLKQKNYWCYGFDASGGTCLHQVEFSERRVVILGAEGKGMRHLTKKHCDHLVKIPMSEKMESLNV
SNAAAIAMYSVFLGDKRS

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=266, Percent_Identity=27.8195488721804, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1790623, Length=239, Percent_Identity=33.0543933054393, Blast_Score=126, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324775, Length=284, Percent_Identity=23.5915492957746, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28401; Mature: 28401

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIKDNRYLWIYGKHACLSALKNPNRVCIELLITHKFIDKNDPVIKLAKNRKIGIRDVE
CCCCCCCCEEEEEECHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEH
IAKIDSLVGSKAIHQGMLLKVLPLFGRNASSLIRIEDIVAQTEAAPTSTILVLDEVTDVH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHCCCCCCEEEEEEECHHHHH
NVGAVLRTAACFNVDAVVLTEHNSPSESCGMAKASSGAIDIVPVVCIGNLVKTLEYLKQK
HHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHC
NYWCYGFDASGGTCLHQVEFSERRVVILGAEGKGMRHLTKKHCDHLVKIPMSEKMESLNV
CCEEEEEECCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHEEECCHHHHHHHCCC
SNAAAIAMYSVFLGDKRS
CCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNRIKDNRYLWIYGKHACLSALKNPNRVCIELLITHKFIDKNDPVIKLAKNRKIGIRDVE
CCCCCCCCEEEEEECHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEH
IAKIDSLVGSKAIHQGMLLKVLPLFGRNASSLIRIEDIVAQTEAAPTSTILVLDEVTDVH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHCCCCCCEEEEEEECHHHHH
NVGAVLRTAACFNVDAVVLTEHNSPSESCGMAKASSGAIDIVPVVCIGNLVKTLEYLKQK
HHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHC
NYWCYGFDASGGTCLHQVEFSERRVVILGAEGKGMRHLTKKHCDHLVKIPMSEKMESLNV
CCEEEEEECCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHEEECCHHHHHHHCCC
SNAAAIAMYSVFLGDKRS
CCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]