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The map label for this gene is rho [H]

Identifier: 88607694

GI number: 88607694

Start: 70587

End: 71945

Strand: Direct

Name: rho [H]

Synonym: APH_0064

Alternate gene names: 88607694

Gene position: 70587-71945 (Clockwise)

Preceding gene: 88607688

Following gene: 88606830

Centisome position: 4.8

GC content: 44.37

Gene sequence:

>1359_bases
GTGTGTGGCAAGGTTAGGAGAAGGAAAAAGGCTGTTCCTAGTGCTGTAGTAGCAGAAGAGGCGACAGGGAGTGGCAGCGA
AGAAGGAAGAATCCTGAACCTATGTGAGCTGAAGAGAAAAAGCACAGGGGAGCTACTAGCAATAGCGGAGGAGCTAGGTG
TAGTAAGCAACGGCAGGATGCTGAAGCAAGAGATAATATTCCAGCTGATGAAGAGAGTAATCAGCGAAGGAGGAGTGGCA
ATAGGTGGTGGTGTAGTAGAAACATTGCCGGATGGATTTGGATTTTTGAGATCAGCAGAAGCAAATTACGCGGCAAGTAG
CGATGACATCTACATATCGGCTGGTCAGATAAAGAAATTTAACTTGAGAACGGGTGACATAGTAAGTGGTGAGATACGTG
CACCTGGAGATAAGGAAAGGTATTTTACCTTAGTCAAGGCGTACAGCATAAACTACACGGAGATAGGTAAGCTACAGAGG
TATGTACACTTTGACGATCTGATTCCAGTATACCCAGAAGATAGAATATTGCTCGAGTGCAATAAGGGTGCTGGGAACGA
GAAGAAAGATATAAGCATGCGTGCTATAGACATAATAGCGCCACTAGGAAAGGGGCAACGTGCGCTAATAGTAGCACCTC
CAAGGGTTGGTAAGACTGTAATCTTGCAGCAAATCGCGCACTCTATAGCTGTAAACCACCCCAACATGGAACTGATAGTG
CTTTTGATAGGTGAAAGGCCAGAAGAAGTCACGGATATGTTGAGGTCAGTAAAGGGAGAAGTTGTAAGTTCTACATTTGA
TGAGCCTGCGTACCGGCACGTACAGTTGGCAGAGATAGTGATAGAAAGAGCTAAGAGGATGGTAGAGCATAAGAAGGAGG
TGGTAATCCTTTTAGACTCTATAACAAGATTAGCTAGGGCATATAATGAGGTTATGCCATCATCAGGAAAGGTGCTTACA
GGTGGGGTAGATTCTAATGCGCTACAGAGGCCTAAGAGGTTTTTTGGAGCAGCTAGGAATATAGAGAATGGGGGATCGCT
CACGATAATAGCGACTGCTCTAATAGAGACGGGATCGAAAATGGATGAAGTGATTTTCGAGGAGTTCAAGGGTACAGGAA
ACTGCGAGATAATACTTGATCGGAAGATAGCAGATAAGAGAATCTACCCAGCGATAGATATATCTAAGTCTGGAACAAGA
AAGGAAGACATGCTGATAGAATCAGCGCTTCTGAAGAAGGTATGGTTACTGAGAAGGTTGTTATCAGCCATGGGTCCAGT
TGAGGCTATGGAGTTTCTGAGAGACAAGCTTAGCATGGCTAAGGATAACAACGACTTTTTTGAAATGATGAATAGCTAA

Upstream 100 bases:

>100_bases
CTTTAGAAGTAAGCATACTTTCGCAGTGCAGGTTTGTTTATATGCTGGGTTCTATCCAGGATCTGGCTTCTTCTGATTGT
GAATTTTTGAAGGTCGTCTT

Downstream 100 bases:

>100_bases
CGGTTATCTGTACGGGTACGATTAGGCGTAACGATGGCTGCAGCATCGTTTGGTGTCTACACCTAGGAAAGACTGGGTAT
GGTTAACTATCCAGAGGTTC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho 1 [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MCGKVRRRKKAVPSAVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRMLKQEIIFQLMKRVISEGGVA
IGGGVVETLPDGFGFLRSAEANYAASSDDIYISAGQIKKFNLRTGDIVSGEIRAPGDKERYFTLVKAYSINYTEIGKLQR
YVHFDDLIPVYPEDRILLECNKGAGNEKKDISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIV
LLIGERPEEVTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDSITRLARAYNEVMPSSGKVLT
GGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKIADKRIYPAIDISKSGTR
KEDMLIESALLKKVWLLRRLLSAMGPVEAMEFLRDKLSMAKDNNDFFEMMNS

Sequences:

>Translated_452_residues
MCGKVRRRKKAVPSAVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRMLKQEIIFQLMKRVISEGGVA
IGGGVVETLPDGFGFLRSAEANYAASSDDIYISAGQIKKFNLRTGDIVSGEIRAPGDKERYFTLVKAYSINYTEIGKLQR
YVHFDDLIPVYPEDRILLECNKGAGNEKKDISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIV
LLIGERPEEVTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDSITRLARAYNEVMPSSGKVLT
GGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKIADKRIYPAIDISKSGTR
KEDMLIESALLKKVWLLRRLLSAMGPVEAMEFLRDKLSMAKDNNDFFEMMNS
>Mature_452_residues
MCGKVRRRKKAVPSAVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRMLKQEIIFQLMKRVISEGGVA
IGGGVVETLPDGFGFLRSAEANYAASSDDIYISAGQIKKFNLRTGDIVSGEIRAPGDKERYFTLVKAYSINYTEIGKLQR
YVHFDDLIPVYPEDRILLECNKGAGNEKKDISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIV
LLIGERPEEVTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDSITRLARAYNEVMPSSGKVLT
GGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKIADKRIYPAIDISKSGTR
KEDMLIESALLKKVWLLRRLLSAMGPVEAMEFLRDKLSMAKDNNDFFEMMNS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=59.6658711217184, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1788251, Length=244, Percent_Identity=28.6885245901639, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 49971; Mature: 49971

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGKVRRRKKAVPSAVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRM
CCCHHHHHHHCCCHHHHHHCCCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHCCCCCCHH
LKQEIIFQLMKRVISEGGVAIGGGVVETLPDGFGFLRSAEANYAASSDDIYISAGQIKKF
HHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCHHHHHCCCCCCCCCCCCEEEECCCEEEE
NLRTGDIVSGEIRAPGDKERYFTLVKAYSINYTEIGKLQRYVHFDDLIPVYPEDRILLEC
ECCCCCEEECCCCCCCCHHHHEEEEHEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
NKGAGNEKKDISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIV
CCCCCCCCCCCHHHHEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEE
LLIGERPEEVTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDS
EEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHH
ITRLARAYNEVMPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSK
HHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCH
MDEVIFEEFKGTGNCEIILDRKIADKRIYPAIDISKSGTRKEDMLIESALLKKVWLLRRL
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LSAMGPVEAMEFLRDKLSMAKDNNDFFEMMNS
HHHHCHHHHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MCGKVRRRKKAVPSAVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRM
CCCHHHHHHHCCCHHHHHHCCCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHCCCCCCHH
LKQEIIFQLMKRVISEGGVAIGGGVVETLPDGFGFLRSAEANYAASSDDIYISAGQIKKF
HHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCHHHHHCCCCCCCCCCCCEEEECCCEEEE
NLRTGDIVSGEIRAPGDKERYFTLVKAYSINYTEIGKLQRYVHFDDLIPVYPEDRILLEC
ECCCCCEEECCCCCCCCHHHHEEEEHEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
NKGAGNEKKDISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIV
CCCCCCCCCCCHHHHEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEE
LLIGERPEEVTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDS
EEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHH
ITRLARAYNEVMPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSK
HHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCH
MDEVIFEEFKGTGNCEIILDRKIADKRIYPAIDISKSGTRKEDMLIESALLKKVWLLRRL
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LSAMGPVEAMEFLRDKLSMAKDNNDFFEMMNS
HHHHCHHHHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA