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The map label for this gene is nusA [H]

Identifier: 88607683

GI number: 88607683

Start: 466398

End: 467942

Strand: Direct

Name: nusA [H]

Synonym: APH_0445

Alternate gene names: 88607683

Gene position: 466398-467942 (Clockwise)

Preceding gene: 88607009

Following gene: 88607319

Centisome position: 31.7

GC content: 44.79

Gene sequence:

>1545_bases
ATGGTGAATTTGTATAATTTTGATAATCTTGAGCTCATAAGAGTTGCCAGGGATGTTGCAGAGCAAAGAGGTCTGGATCT
TGGTGTAATAGTAGAAGCTATAGAGCAAGCGCTTGAGGTTGTGAGTCATGTTAAGTATGGGGATAGTAAGATTAAGGTCT
CCATTGATAGGAAGACGGGGGTGATATCTGTAGCTCGGCAGGTTCTGGTAGTGAAGGATGACTTCACTTTTGATAGTGAA
GAGTGTGGTATAGCGGCTGAAGAGGTTAATAAGTATAAGCTTGTGAGAATCTCTGATGCGTTGGCTGACGGGGAGCAGGT
TGAGGTTGGTGATATACTGTTGGAGCCGTTGCCGCCGATTGATATTGATTATAATTCTGCGAAGCTCGCAAAGCAAAAGG
TTGCGCAGCTAATAATGATGCAGGAAAGGAAGAGGCAATACGAGGAGTTCAAGGATCGAGTTGGTGATCTTGTATATGGG
GTTGTGAAGCGTGTTGAGTACAACAACATTATTGTAGATCTAAACGGGAGCGAGGGGTATCTTCCTGTATACAATACTAT
TAGGGGGGAAGTATTTCGTCCTAATGCTAGGGTCAAGGCGCATGTAGAGGATGTGCGTAGGGAAGCTACGGGGCCTCAGA
TTTTTCTTTCGAGGGTTAGTAAGAGGTTTATGGAGCTTCTGTTTAAGCAGGAGATTTCAGAAGTATATGATGGTATCGTT
ACTATAAAGGCCTTGGCTAGGGATGCTGGTTCTAGGTCTAAGGTTGCGGTTTATTCGTCTGATAAGAATGTGGATCCAGT
TGGGGCGTGTATTGGTGCTCGTGGAGTTAGGATACAGAATATAGTTGCGGAGCTCAACGGAGAGAAGATAGATGTAATTC
CGTATTCTCCGGATCTTGCCAAGTTTGTGGTTAGTGCGATTGCGCCTGCGGAAGTGGTGAAGGTGATTATCGACGAAGAT
GTAGAAAAGATAGAGCTGGTGGTACCTGAGAGCCAAGTGAGTTTGGCTATAGGTAGATATGGGCAAAATATAAGGTTGGC
TTCAGAGCTGGTTGGCTGGAGTATTGATGTAATTGGGGATGAGACTGAATCGACTAGGAAGGCTAAGGAGTTGAGTAGTG
GTGCCAAGGTCTTTGTAGAGGATTTGGATGTTGAGGAAATTATAGGGCAGCTTCTTGTCAGTGAGGGTTTTTCTAGTATT
GAAGATCTAGATAGAGCTGAGGTTGGCGATATTGCGGCTGTTGATGGCTTCAATGAAGAGATAGCGCGAGAGTTGAAGAC
GAGGGCGGCTGAGTGTCTGGCGCGCAAGCGTAAGGAAGCTATTGAGATGCTTGAAAGCATGTCGGTGAGTGAAGAGGTTA
TGCAACTTCCGTTCTTGCAGGTAGAAGATATCGTAAAGTTAAGTGAGAATGGCGTGAGGAGCGTAGAAGATATAGCTTCT
CTTTGTACTGATGAATTTTATGATATTGTTCCCAGGGCTAAGTTAAGTAAAGAGCAAGTGGATTCCGTCATTCTGGAATC
ACGCAAACGAGTTGGGTGGGTTTAG

Upstream 100 bases:

>100_bases
ATTGGTATCGTAAGTAGAATCCCCGTGGCAGACTAGTATGGAGCACTGTCGCTAATGGCATGGTGTGTGATAGGTGTTTG
CTACTCGTGCAAGGGGTTTT

Downstream 100 bases:

>100_bases
TTTTCCAGGTACTGCAGATGAGTGATGTTGAAAAGGTTGATGGGGAGTGTAGTGCTTCGGGTAAACGGGAGCGTTTGACG
CTTAAGCTTGGTGATAAGCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MVNLYNFDNLELIRVARDVAEQRGLDLGVIVEAIEQALEVVSHVKYGDSKIKVSIDRKTGVISVARQVLVVKDDFTFDSE
ECGIAAEEVNKYKLVRISDALADGEQVEVGDILLEPLPPIDIDYNSAKLAKQKVAQLIMMQERKRQYEEFKDRVGDLVYG
VVKRVEYNNIIVDLNGSEGYLPVYNTIRGEVFRPNARVKAHVEDVRREATGPQIFLSRVSKRFMELLFKQEISEVYDGIV
TIKALARDAGSRSKVAVYSSDKNVDPVGACIGARGVRIQNIVAELNGEKIDVIPYSPDLAKFVVSAIAPAEVVKVIIDED
VEKIELVVPESQVSLAIGRYGQNIRLASELVGWSIDVIGDETESTRKAKELSSGAKVFVEDLDVEEIIGQLLVSEGFSSI
EDLDRAEVGDIAAVDGFNEEIARELKTRAAECLARKRKEAIEMLESMSVSEEVMQLPFLQVEDIVKLSENGVRSVEDIAS
LCTDEFYDIVPRAKLSKEQVDSVILESRKRVGWV

Sequences:

>Translated_514_residues
MVNLYNFDNLELIRVARDVAEQRGLDLGVIVEAIEQALEVVSHVKYGDSKIKVSIDRKTGVISVARQVLVVKDDFTFDSE
ECGIAAEEVNKYKLVRISDALADGEQVEVGDILLEPLPPIDIDYNSAKLAKQKVAQLIMMQERKRQYEEFKDRVGDLVYG
VVKRVEYNNIIVDLNGSEGYLPVYNTIRGEVFRPNARVKAHVEDVRREATGPQIFLSRVSKRFMELLFKQEISEVYDGIV
TIKALARDAGSRSKVAVYSSDKNVDPVGACIGARGVRIQNIVAELNGEKIDVIPYSPDLAKFVVSAIAPAEVVKVIIDED
VEKIELVVPESQVSLAIGRYGQNIRLASELVGWSIDVIGDETESTRKAKELSSGAKVFVEDLDVEEIIGQLLVSEGFSSI
EDLDRAEVGDIAAVDGFNEEIARELKTRAAECLARKRKEAIEMLESMSVSEEVMQLPFLQVEDIVKLSENGVRSVEDIAS
LCTDEFYDIVPRAKLSKEQVDSVILESRKRVGWV
>Mature_514_residues
MVNLYNFDNLELIRVARDVAEQRGLDLGVIVEAIEQALEVVSHVKYGDSKIKVSIDRKTGVISVARQVLVVKDDFTFDSE
ECGIAAEEVNKYKLVRISDALADGEQVEVGDILLEPLPPIDIDYNSAKLAKQKVAQLIMMQERKRQYEEFKDRVGDLVYG
VVKRVEYNNIIVDLNGSEGYLPVYNTIRGEVFRPNARVKAHVEDVRREATGPQIFLSRVSKRFMELLFKQEISEVYDGIV
TIKALARDAGSRSKVAVYSSDKNVDPVGACIGARGVRIQNIVAELNGEKIDVIPYSPDLAKFVVSAIAPAEVVKVIIDED
VEKIELVVPESQVSLAIGRYGQNIRLASELVGWSIDVIGDETESTRKAKELSSGAKVFVEDLDVEEIIGQLLVSEGFSSI
EDLDRAEVGDIAAVDGFNEEIARELKTRAAECLARKRKEAIEMLESMSVSEEVMQLPFLQVEDIVKLSENGVRSVEDIAS
LCTDEFYDIVPRAKLSKEQVDSVILESRKRVGWV

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=507, Percent_Identity=32.5443786982249, Blast_Score=268, Evalue=6e-73,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 57199; Mature: 57199

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLYNFDNLELIRVARDVAEQRGLDLGVIVEAIEQALEVVSHVKYGDSKIKVSIDRKTG
CCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
VISVARQVLVVKDDFTFDSEECGIAAEEVNKYKLVRISDALADGEQVEVGDILLEPLPPI
HHHHHHHHEEEECCCCCCCHHCCCCHHHCCCEEEEEEHHHHCCCCCEEEHHHHCCCCCCC
DIDYNSAKLAKQKVAQLIMMQERKRQYEEFKDRVGDLVYGVVKRVEYNNIIVDLNGSEGY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCC
LPVYNTIRGEVFRPNARVKAHVEDVRREATGPQIFLSRVSKRFMELLFKQEISEVYDGIV
EEHHHCCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIKALARDAGSRSKVAVYSSDKNVDPVGACIGARGVRIQNIVAELNGEKIDVIPYSPDLA
HHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCHHH
KFVVSAIAPAEVVKVIIDEDVEKIELVVPESQVSLAIGRYGQNIRLASELVGWSIDVIGD
HHHHHHHCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCEEHHHHHHCCEEEEECC
ETESTRKAKELSSGAKVFVEDLDVEEIIGQLLVSEGFSSIEDLDRAEVGDIAAVDGFNEE
CCHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCEEECCCCCHH
IARELKTRAAECLARKRKEAIEMLESMSVSEEVMQLPFLQVEDIVKLSENGVRSVEDIAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHH
LCTDEFYDIVPRAKLSKEQVDSVILESRKRVGWV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVNLYNFDNLELIRVARDVAEQRGLDLGVIVEAIEQALEVVSHVKYGDSKIKVSIDRKTG
CCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
VISVARQVLVVKDDFTFDSEECGIAAEEVNKYKLVRISDALADGEQVEVGDILLEPLPPI
HHHHHHHHEEEECCCCCCCHHCCCCHHHCCCEEEEEEHHHHCCCCCEEEHHHHCCCCCCC
DIDYNSAKLAKQKVAQLIMMQERKRQYEEFKDRVGDLVYGVVKRVEYNNIIVDLNGSEGY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCC
LPVYNTIRGEVFRPNARVKAHVEDVRREATGPQIFLSRVSKRFMELLFKQEISEVYDGIV
EEHHHCCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIKALARDAGSRSKVAVYSSDKNVDPVGACIGARGVRIQNIVAELNGEKIDVIPYSPDLA
HHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCHHH
KFVVSAIAPAEVVKVIIDEDVEKIELVVPESQVSLAIGRYGQNIRLASELVGWSIDVIGD
HHHHHHHCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCEEHHHHHHCCEEEEECC
ETESTRKAKELSSGAKVFVEDLDVEEIIGQLLVSEGFSSIEDLDRAEVGDIAAVDGFNEE
CCHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCEEECCCCCHH
IARELKTRAAECLARKRKEAIEMLESMSVSEEVMQLPFLQVEDIVKLSENGVRSVEDIAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHH
LCTDEFYDIVPRAKLSKEQVDSVILESRKRVGWV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]