Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 88607600

GI number: 88607600

Start: 346747

End: 348723

Strand: Direct

Name: priA [H]

Synonym: APH_0356

Alternate gene names: 88607600

Gene position: 346747-348723 (Clockwise)

Preceding gene: 88607709

Following gene: 88607125

Centisome position: 23.57

GC content: 42.64

Gene sequence:

>1977_bases
ATGATTGCTGAGGTGCTACTTCCTATACCATTGGATAGGCCATTTTACTATTTGGTCCCAGACGAAATTCAAATTTCAGT
AGGTGATTATGTGCGTGTACCCTTTGGCAATAGGGTTGCGTTAGGGTTGGTTACGGATCTTAAAAATAGCATCGAATCTG
ATTTAGAGTTGAAGTACATCAAAGATAAACTTATTTTGCCCTCCATGGCTCCGTCGTTTATCAAATTTATTCAGTGGGTT
AGCAATTACAACATTGTACCCATAGGTATGGTATTGAAGATGGTATTTGCAGGTATGCCTAGGGGTAAATTTATGCCGCT
AGGTGGTGATTTAGCGCAGAGTACGAGCGTTAATGATGTGAATATAGAAGCGGGAAAATTGCCGCAGCCATCTGAAGACC
AAAGTTATGCGTGTAACTATATAGTAGAGCGTTCTACAGGGTTTTCAGTCACTGTATTGGATGGGAAGACAGGTGCTGGG
AAAACAGAAGTTTATTGTACTGCTGCGGAGAAGCTCTTACAGGAATGTGCAGATGCTCAGGTACTGGTACTCCTTCCTGA
AATTGTTTTAGCAACGCAGCTCATGAAGCGGATTTACAGCTATTTTTCCACTTGTAATCCAGTGGAGTGGCATTCTGAAT
TGACGGTAAAAAGGCGTAGGGAGAATTGGCTTGCAGTGACGCGTGGTACTACTTCTATAGTTGTGGGGGCGAGATCTGCG
TTATTTTTGCCCTTTAAAAATCTGAAGATGATTATTGTCGATGAAGAGCACGAGAGCTCATACAAACAGGATAGCGGCAT
GATATACAATGCGCGAGACATGGCCATTGTATTGGGAAAGCACGTTGATATTCCAGTAGTGTTGTGTACAGCGACTCCAT
CACTGGAAACCATGAATAACATACAGCAAAAGAAGTACAATCATGTAGTATTGAAGAGAAGGTTCGGAGAAGCGGTGATG
CCAGATATAATAGTGGCGGATATGCGCAAAGACGAACTAAAAAAGGCATGGATGTCTACATGCTTATACGAAAAGATATG
TGAAACACTTGCAAAACAGCAGCAAGTAATGTTGTTCTTGAACCGACGGGGATATGCAAGGCTTGTACTATGCAAACAAT
GCGGACACAAAGTAAACTGTCCAAACTGTTGTACGTGGCTTACGGAGCACAGAGTTTTAAGGGCAATGCTATGCCACTAC
TGTGCGTATAGTTGCGAGATACCAAGAGAGTGTCCATCGTGTCAAGAGTATAACACCATGAGTCCATATGGCGTGGGTAT
AGAAAGGATACTAGAGGGTATACAAGAACTCATTCCTACGGCGCGCTCTGTGGTCCTAAGCAGTGATATAAGTGCCAAAC
AGGCGAATAGTATCGTTGAGCAAGTACTGCAAGGAGAAGTCGATATTATTATTGGTACGCAGATTATAGCAAAGGGGCAT
AACTTTCCTAAATTGACGTTGGTAGGTGTTATAGATGCGGACTTAGGGTTGGGAAACAGTGATCTCAGGGCAACAGAAAA
GACGTATCAGCTGTTGCATCAGGTTTCGGGCCGTTCTGGGAGATATAAAGAAAAGGGGCAGGTTGTACTACAGACGTATG
ATCCAGAGAGTGCGGTTATAAAATCGTTGCTGTCCAGTAATCGAGATGATTTTTATAGTGTAGAGCTAAAAACAAGAAAA
GTAGCTGAAATGCCTCCGTACATGAGGTTAGTATCAATAATAATCTCAGGGAGAGAAGAAATAGGTGTGATTGCTGTGGC
TAATGAGATTGTCGCGAGCATGGCTGGTAAAATAACAGTTTGGGGACCGGCTCCAGCGCCTCTTAGCTTATTAAACAACA
TGCACAGGTATAGGGTGCTTTTGAAAGTTACCAGTATTTCAGCGATTAAGAGCTTACTGTCAGAGTGCAGAGAGAAATAC
AAAAAGTTGCGTAATGTACGTGTCGTTATCGATGTGGACCCCATGAACTTTATTTAG

Upstream 100 bases:

>100_bases
CCTATGTGTTATAGTAAGCGCTGGTGTTTTTGCGGTAAGTTTTCTTAGGGAGTTCCGGGGGTATGGCCAGCGAAGTTTGT
AGAGAGGTAGGTAGAGCATT

Downstream 100 bases:

>100_bases
ATAGGTTTGCTGTGGGATATTTTTTAGAAGATTTGTATAGTGTAGGAGTGGGATATAGCATGCGGTGTAGCTCCCGCTTT
TAGAATTAGTGAGTTTATAT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MIAEVLLPIPLDRPFYYLVPDEIQISVGDYVRVPFGNRVALGLVTDLKNSIESDLELKYIKDKLILPSMAPSFIKFIQWV
SNYNIVPIGMVLKMVFAGMPRGKFMPLGGDLAQSTSVNDVNIEAGKLPQPSEDQSYACNYIVERSTGFSVTVLDGKTGAG
KTEVYCTAAEKLLQECADAQVLVLLPEIVLATQLMKRIYSYFSTCNPVEWHSELTVKRRRENWLAVTRGTTSIVVGARSA
LFLPFKNLKMIIVDEEHESSYKQDSGMIYNARDMAIVLGKHVDIPVVLCTATPSLETMNNIQQKKYNHVVLKRRFGEAVM
PDIIVADMRKDELKKAWMSTCLYEKICETLAKQQQVMLFLNRRGYARLVLCKQCGHKVNCPNCCTWLTEHRVLRAMLCHY
CAYSCEIPRECPSCQEYNTMSPYGVGIERILEGIQELIPTARSVVLSSDISAKQANSIVEQVLQGEVDIIIGTQIIAKGH
NFPKLTLVGVIDADLGLGNSDLRATEKTYQLLHQVSGRSGRYKEKGQVVLQTYDPESAVIKSLLSSNRDDFYSVELKTRK
VAEMPPYMRLVSIIISGREEIGVIAVANEIVASMAGKITVWGPAPAPLSLLNNMHRYRVLLKVTSISAIKSLLSECREKY
KKLRNVRVVIDVDPMNFI

Sequences:

>Translated_658_residues
MIAEVLLPIPLDRPFYYLVPDEIQISVGDYVRVPFGNRVALGLVTDLKNSIESDLELKYIKDKLILPSMAPSFIKFIQWV
SNYNIVPIGMVLKMVFAGMPRGKFMPLGGDLAQSTSVNDVNIEAGKLPQPSEDQSYACNYIVERSTGFSVTVLDGKTGAG
KTEVYCTAAEKLLQECADAQVLVLLPEIVLATQLMKRIYSYFSTCNPVEWHSELTVKRRRENWLAVTRGTTSIVVGARSA
LFLPFKNLKMIIVDEEHESSYKQDSGMIYNARDMAIVLGKHVDIPVVLCTATPSLETMNNIQQKKYNHVVLKRRFGEAVM
PDIIVADMRKDELKKAWMSTCLYEKICETLAKQQQVMLFLNRRGYARLVLCKQCGHKVNCPNCCTWLTEHRVLRAMLCHY
CAYSCEIPRECPSCQEYNTMSPYGVGIERILEGIQELIPTARSVVLSSDISAKQANSIVEQVLQGEVDIIIGTQIIAKGH
NFPKLTLVGVIDADLGLGNSDLRATEKTYQLLHQVSGRSGRYKEKGQVVLQTYDPESAVIKSLLSSNRDDFYSVELKTRK
VAEMPPYMRLVSIIISGREEIGVIAVANEIVASMAGKITVWGPAPAPLSLLNNMHRYRVLLKVTSISAIKSLLSECREKY
KKLRNVRVVIDVDPMNFI
>Mature_658_residues
MIAEVLLPIPLDRPFYYLVPDEIQISVGDYVRVPFGNRVALGLVTDLKNSIESDLELKYIKDKLILPSMAPSFIKFIQWV
SNYNIVPIGMVLKMVFAGMPRGKFMPLGGDLAQSTSVNDVNIEAGKLPQPSEDQSYACNYIVERSTGFSVTVLDGKTGAG
KTEVYCTAAEKLLQECADAQVLVLLPEIVLATQLMKRIYSYFSTCNPVEWHSELTVKRRRENWLAVTRGTTSIVVGARSA
LFLPFKNLKMIIVDEEHESSYKQDSGMIYNARDMAIVLGKHVDIPVVLCTATPSLETMNNIQQKKYNHVVLKRRFGEAVM
PDIIVADMRKDELKKAWMSTCLYEKICETLAKQQQVMLFLNRRGYARLVLCKQCGHKVNCPNCCTWLTEHRVLRAMLCHY
CAYSCEIPRECPSCQEYNTMSPYGVGIERILEGIQELIPTARSVVLSSDISAKQANSIVEQVLQGEVDIIIGTQIIAKGH
NFPKLTLVGVIDADLGLGNSDLRATEKTYQLLHQVSGRSGRYKEKGQVVLQTYDPESAVIKSLLSSNRDDFYSVELKTRK
VAEMPPYMRLVSIIISGREEIGVIAVANEIVASMAGKITVWGPAPAPLSLLNNMHRYRVLLKVTSISAIKSLLSECREKY
KKLRNVRVVIDVDPMNFI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=537, Percent_Identity=35.9404096834264, Blast_Score=335, Evalue=7e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 73740; Mature: 73740

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAEVLLPIPLDRPFYYLVPDEIQISVGDYVRVPFGNRVALGLVTDLKNSIESDLELKYI
CCCCEEECCCCCCCEEEECCCEEEEEECCEEECCCCCEEEEHHHHHHHHHHHCCCEEEEH
KDKLILPSMAPSFIKFIQWVSNYNIVPIGMVLKMVFAGMPRGKFMPLGGDLAQSTSVNDV
HHHHCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCEECCCCCHHCCCCCCEE
NIEAGKLPQPSEDQSYACNYIVERSTGFSVTVLDGKTGAGKTEVYCTAAEKLLQECADAQ
EECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHCCCE
VLVLLPEIVLATQLMKRIYSYFSTCNPVEWHSELTVKRRRENWLAVTRGTTSIVVGARSA
EEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCE
LFLPFKNLKMIIVDEEHESSYKQDSGMIYNARDMAIVLGKHVDIPVVLCTATPSLETMNN
EEEECCCCEEEEEECCHHHCCCCCCCCEEECCCEEEEECCCCCCCEEEEECCCCHHHHHH
IQQKKYNHVVLKRRFGEAVMPDIIVADMRKDELKKAWMSTCLYEKICETLAKQQQVMLFL
HHHHHHHHHHHHHHCCCHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
NRRGYARLVLCKQCGHKVNCPNCCTWLTEHRVLRAMLCHYCAYSCEIPRECPSCQEYNTM
CCCCCEEEHEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SPYGVGIERILEGIQELIPTARSVVLSSDISAKQANSIVEQVLQGEVDIIIGTQIIAKGH
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECEEEECCC
NFPKLTLVGVIDADLGLGNSDLRATEKTYQLLHQVSGRSGRYKEKGQVVLQTYDPESAVI
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHH
KSLLSSNRDDFYSVELKTRKVAEMPPYMRLVSIIISGREEIGVIAVANEIVASMAGKITV
HHHHHCCCCCEEEEEEEHHHHHCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCEEE
WGPAPAPLSLLNNMHRYRVLLKVTSISAIKSLLSECREKYKKLRNVRVVIDVDPMNFI
ECCCCCHHHHHCCHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
>Mature Secondary Structure
MIAEVLLPIPLDRPFYYLVPDEIQISVGDYVRVPFGNRVALGLVTDLKNSIESDLELKYI
CCCCEEECCCCCCCEEEECCCEEEEEECCEEECCCCCEEEEHHHHHHHHHHHCCCEEEEH
KDKLILPSMAPSFIKFIQWVSNYNIVPIGMVLKMVFAGMPRGKFMPLGGDLAQSTSVNDV
HHHHCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCEECCCCCHHCCCCCCEE
NIEAGKLPQPSEDQSYACNYIVERSTGFSVTVLDGKTGAGKTEVYCTAAEKLLQECADAQ
EECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHCCCE
VLVLLPEIVLATQLMKRIYSYFSTCNPVEWHSELTVKRRRENWLAVTRGTTSIVVGARSA
EEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCE
LFLPFKNLKMIIVDEEHESSYKQDSGMIYNARDMAIVLGKHVDIPVVLCTATPSLETMNN
EEEECCCCEEEEEECCHHHCCCCCCCCEEECCCEEEEECCCCCCCEEEEECCCCHHHHHH
IQQKKYNHVVLKRRFGEAVMPDIIVADMRKDELKKAWMSTCLYEKICETLAKQQQVMLFL
HHHHHHHHHHHHHHCCCHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
NRRGYARLVLCKQCGHKVNCPNCCTWLTEHRVLRAMLCHYCAYSCEIPRECPSCQEYNTM
CCCCCEEEHEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SPYGVGIERILEGIQELIPTARSVVLSSDISAKQANSIVEQVLQGEVDIIIGTQIIAKGH
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECEEEECCC
NFPKLTLVGVIDADLGLGNSDLRATEKTYQLLHQVSGRSGRYKEKGQVVLQTYDPESAVI
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHH
KSLLSSNRDDFYSVELKTRKVAEMPPYMRLVSIIISGREEIGVIAVANEIVASMAGKITV
HHHHHCCCCCEEEEEEEHHHHHCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCEEE
WGPAPAPLSLLNNMHRYRVLLKVTSISAIKSLLSECREKYKKLRNVRVVIDVDPMNFI
ECCCCCHHHHHCCHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]